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Acot12 (NM_028790) Mouse Tagged ORF Clone
CAT＃: MR224284
- TrueORF®
Acot12 (Myc-DDK-tagged) - Mouse acyl-CoA thioesterase 12 (Acot12)
ORF Plasmid: tGFP
"NM_028790" in other vectors (4)
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CNY 4,144.00
CNY 4,940.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1300004O04Rik; 4930449F15Rik; AV027244; Cach; mCACH-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224284 representing NM_028790
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCGATGGTGGCGCCGGGTGAGGTGCTCATGAGCCAGGCCATCCAGCCGGCTCATGCCGACTCCC GCGGCGAGCTGAGCGCAGGGCAGCTGCTCAAGTGGATGGACACCACCGCTTGCCTGGCGGCCGAAAAGCA TGCTGGGATTTCCTGTGTCACAGCCTCCATGGATGACATTCTGTTTGAGGACACAGCGAGAATTGGACAA ATTATTACCATCAGAGCAAAAGTGACTAGGGCGTTCAGCACAAGCATGGAGATCAGTATCAAGGTCATAG TCCAGGACAAGTTCACGGGCATCCAGAAGCTCCTCTGCGTGGCTTTCTCTACGTTTGTAGCTAAACCAGT TGGCAAAGAAAAGGTTCACTTAAAACCTGTCCTGCTTCAAACAGAGCAAGAACAAGTGGAGCACAATCTG GCTTCAGAGAGAAGGAAAGTCCGACTGCAGCATGAGAACACCTTCAACAACATTATGAAGGAGAGCAGCA GGTTCAGCGATTCCATTTGTAATGAAGAAGAAGGAACGGCCACCACCATGGGCACCTCTGTCCAGAGCAT CGAGCTTGTCCTTCCGCCCCACGCAAACCATCACGGAAACACATTTGGTGGCCAGATCATGGCATGGATG GAGACAGTCGCCACCATTTCTGCAAGCCGCCTGTGTCATGGGCATCCCTTTCTGAAGTCTGTGGATATGT TTAAATTCCGGGGACCGTCCACAGTTGGAGACCGCCTTGTCTTCAGTGCCATAGTCAACAACACCTTTCA GAACAGTGTGGAAGTCGGAGTGCGTGTTGAGGCCTTTGACTGTCAGGAGTGGGCCGAGGGCCAAGGCCGC CACATCAACAGCGCTTTTCTCATTTACAATGCTGTTGATGACCAGGAGAAACTCATCACCTTTCCCAGAA TCCAACCCATTTCAAAGGACGATTTTCGCCGTTACCAGGGAGCCATCGCACGGAGGAGAATTCGCCTAGG CAGGAAATATGTTATTTCCCACAAGAAAGAAGTTCCACTCAGTGCACAGTGGGATATAAGCAAAAAGGGA TCCCTAAGTAACACCAATGTGGAAGCTCTCAAAAATCTGGCATCCAAAAGCGGTTGGGAGATTACCACCA CCTTGGAGAAGATAAAAATATATACCCTGGAGGAGCAAGATGCCATATCTGTTAAGGTTGAAAAGCTTGT GGGCAGTCCAGCCCACATAGCTTATCATCTCTTGTCTGACCTCACAAAGCGACCTTTATGGGACCCCCAT TACATATCTTGTGAAGTTATAGACCAGGTGAGCGAGGACGATCAGATATATTACATCACTTGCTCGGTGG TAAATGGAGACAAACCCAAGGACTTTGTGGTGCTTGTGTCACGAAGAAAGCCCCTCAAAGACAACAACAC CTACACCGTGGCACTAAGGTCAGTTGTGCTGCCGTCTGTCCCGTCATCTCCACAGTACATCAGAAGTGAG GTCATTTGTGCTGGGTTTCTCATCCAGGCTGTCGACAGCAATTCGTGCACCGTAACGTACCTGAACCAGA TGTCAGACAGCATCCTCCCTTACTTTGCTGGCAATATTGGTGGCTGGTCAAAATCCATTGAGGAAGCTGC AGCCTCTTGTATAAAATTCATAGAGAATGCTACTCCTGATGGACTTAAAAGTGTGTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224284 representing NM_028790
Red=Cloning site Green=Tags(s) MESMVAPGEVLMSQAIQPAHADSRGELSAGQLLKWMDTTACLAAEKHAGISCVTASMDDILFEDTARIGQ IITIRAKVTRAFSTSMEISIKVIVQDKFTGIQKLLCVAFSTFVAKPVGKEKVHLKPVLLQTEQEQVEHNL ASERRKVRLQHENTFNNIMKESSRFSDSICNEEEGTATTMGTSVQSIELVLPPHANHHGNTFGGQIMAWM ETVATISASRLCHGHPFLKSVDMFKFRGPSTVGDRLVFSAIVNNTFQNSVEVGVRVEAFDCQEWAEGQGR HINSAFLIYNAVDDQEKLITFPRIQPISKDDFRRYQGAIARRRIRLGRKYVISHKKEVPLSAQWDISKKG SLSNTNVEALKNLASKSGWEITTTLEKIKIYTLEEQDAISVKVEKLVGSPAHIAYHLLSDLTKRPLWDPH YISCEVIDQVSEDDQIYYITCSVVNGDKPKDFVVLVSRRKPLKDNNTYTVALRSVVLPSVPSSPQYIRSE VICAGFLIQAVDSNSCTVTYLNQMSDSILPYFAGNIGGWSKSIEEAAASCIKFIENATPDGLKSVL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_028790 |
| ORF Size | 1668 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_028790.3, NP_083066.1 |
| RefSeq Size | 3049 bp |
| RefSeq ORF | 1671 bp |
| Locus ID | 74156 |
| UniProt ID | Q9DBK0 |
| MW | 61.8 kDa |
| Gene Summary | Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Acyl-coenzyme A thioesterase 12/ACOT12 preferentially hydrolyzes acetyl-CoA.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218984 | Acot12 (untagged) - Mouse acyl-CoA thioesterase 12 (Acot12), (10ug) |
CNY 7,130.00 |
|
| MG224284 | Acot12 (tGFP-tagged) - Mouse acyl-CoA thioesterase 12 (Acot12), (10ug) |
CNY 5,420.00 |
|
| MR224284L3 | Lenti ORF clone of Acot12 (Myc-DDK-tagged) - Mouse acyl-CoA thioesterase 12 (Acot12) |
CNY 6,840.00 |
|
| MR224284L4 | Lenti ORF clone of Acot12 (mGFP-tagged) - Mouse acyl-CoA thioesterase 12 (Acot12) |
CNY 6,840.00 |
