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Rock1 (NM_009071) Mouse Tagged ORF Clone
CAT＃: MR224143
- TrueORF®
Rock1 (Myc-DDK-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 1 (Rock1)
ORF Plasmid: tGFP
"NM_009071" in other vectors (4)
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CNY 9,264.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110055K06Rik; Rock-I |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224143 representing NM_009071
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGACTGGGGACAGTTTTGAGACTCGGTTTGAAAAAATCGACAACCTGCTGCGGGATCCCAAATCGG AAGTGAATTCAGATTGTTTGCTGGATGGATTGGATGCCCTGGTTTATGATTTGGATTTTCCTGCCTTAAG AAAAAACAAAAATATTGACAACTTTCTAAGCAGATATAAAGACACAATAAATAAAATTAGAGATTTACGG ATGAAAGCAGAAGATTATGAAGTGGTAAAGGTAATCGGCAGAGGTGCATTTGGAGAAGTTCAATTGGTGA GGCATAAATCCACTAGGAAGGTTTATGCTATGAAGCTGCTCAGCAAATTTGAAATGATTAAGAGATCGGA TTCTGCTTTTTTCTGGGAAGAAAGGGACATCATGGCGTTTGCCAATAGTCCTTGGGTTGTTCAGCTTTTT TATGCATTCCAAGATGACCGGTATCTCTACATGGTGATGGAGTACATGCCTGGCGGAGACCTTGTCAACC TGATGAGCAACTATGACGTGCCTGAAAAATGGGCAAGATTCTATACTGCAGAAGTAGTTCTTGCATTGGA TGCAATCCATTCCATGGGTTTTATTCATAGAGATGTAAAGCCTGATAACATGCTGCTGGATAAGTCTGGA CATTTGAAGTTAGCAGATTTTGGTACTTGTATGAAGATGAATAAGGAAGGTATGGTACGATGTGACACAG CAGTTGGAACACCTGATTATATCTCACCCGAAGTATTAAAATCCCAAGGCGGTGATGGCTATTATGGACG AGAGTGTGACTGGTGGTCAGTTGGGGTATTTCTATATGAAATGCTCGTAGGTGACACACCTTTCTATGCA GATTCTTTGGTTGGGACTTACAGTAAAATTATGAACCATAAAAATTCACTTACTTTCCCTGATGATAATG ACATATCAAAAGAGGCAAAAAATCTCATTTGTGCCTTCCTTACAGACAGAGAAGTGAGATTGGGCAGAAA TGGTGTAGAGGAAATCAAACGTCATCTCTTCTTCAAAAATGATCAGTGGGCTTGGGAAACGCTCCGAGAC ACTGTAGCACCAGTTGTGCCTGATTTAAGTAGTGACATTGATACTAGTAATTTTGATGACTTAGAAGAAG ATAAAGGAGATGAAGAAACATTTCCTATTCCAAAAGCTTTTGTTGGCAATCAGCTACCTTTTGTAGGATT TACATATTATAGCAATCGTAGATACTTACCTTCAGCAAATGCCAGTGAGAACAGATCTAGCTCCAATGTG GATAAAAGCTTGCAGGAAAGTTTACAGAAAACAATCTACAAGCTTGAAGAGCAACTGCATAATGAAATGC AATTAAAAGATGAAATGGAGCAGAAATGCAGAACTTCAAACTTAAAACTAGACAAGATAATGAAAGAATT GGATGAAGAGGGGAATCAAAGAAGAAATCTAGAATCTGCAGTGTCTCAAATTGAGAAAGAAAAGATGTTG CTACAACATAGAATTAATGAGTACCAAAGAAAAGTGGAACAGGAAAATGAGAAGAGAAGAAATATAGAAA ATGAAGTTTCTACACTAAAGGACCAGTTAGAAGACTTACGGAAAGCAAGTCAGACCTCACAGCTTGCTAA TGAGAAGCTGACACAGTTACAGAAGCAGTTAGAAGAAGCGAATGACTTACTTAGGACAGAATCAGACACA GCTGTAAGATTAAGGAAGAGTCACACAGAGATGAGCAAGTCAATTAGTCAGCTAGAGTCCCTGAACAGAG AATTACAGGAAAGAAACAGAATTTTAGAGAATTCCAAGTCACAAGCAGACAAGGATTACTACCAGCTGCA AGCTGTATTGGAAGCTGAGCGAAGAGACAGAGGGCATGATTCTGAGATGATTGGAGACCTTCAAGCACGA ATTACATCTTTACAAGAAGAAGTGAAACATCTCAAACATAATCTTGAAAGAGTGGAAGGAGAAAGAAAAG AGGCTCAAGACATGCTTAATCATTCAGAAAAGGAAAAGAATAATTTGGAGATAGATTTGAATTACAAGCT CAAGTCTATACAACAGCGGTTAGAACAAGAAGTAAATGAACATAAAGTCACCAAAGCACGCCTAACTGAC AAGCACCAATCTATCGAAGAGGCAAAGTCTGTTGCCATGTGTGAAATGGAAAAAAAGCTGAAGGAAGAGA GAGAAGCTCGAGAGAAGGCAGAAAATAGAGTTGTTGAGACTGAGAAACAATGTTCCATGCTAGATGTTGA CCTGAAACAGTCTCAGCAGAAGCTAGAACATTTGACCGAAAATAAAGAAAGGATGGAGGATGAAGTTAAG AATCTCGCCCTGCAGCTGGAACAGGAATCAAATAAACGGCTCTTGTTACAGAATGAATTGAAGACTCAAG CATTTGAAGCAGACAATTTAAAAGGTTTAGAAAAACAGATGAAACAGGAAATAAATACTTTATTGGAAGC AAAGAGATTGTTAGAATTTGAGTTAGCTCAGCTTACAAAGCAATATAGGGGAAATGAAGGGCAAATGCGG GAGTTACAAGATCAACTTGAAGCTGAGCAGTATTTCTCTACACTTTATAAAACCCAAGTAAAGGAGCTTA AAGAAGAAATTGAAGAAAAAAACCGAGAAAATTTAAGGAAAATACAGGAACTGCAAAGTGAAAAAGAAAC TCTCTCTACTCAGTTGGATTTAGCAGAAACAAAAGCTGAGTCTGAGCAGCTGGCGCGAGGGATTCTGGAA GAACAGTATTTTGAGTTGACTCAGGAAAGCAAGAAAGCTGCTTCAAGAAATAGACAAGAGATTACAGATA AAGATCATACTGTTAGTCGGCTTGAAGAAACAAACAGTGTGCTAACCAAAGATATTGAAATGCTAAGAAA AGAAAATGAAGAGCTAAATGAAAGAATGAGGACAGCAGAAGAAGAATATAAATTGAAGAAGGAGGAAGAA ATAAATAATCTTAAGGCTGCTTTTGAAAAGAATATCAGTACCGAACGGACCCTTAAAACACAGGCTGTTA ACAAGTTGGCAGAAATAATGAATCGCAAAGATTTTAAAATTGATCGAAAGAAAGCTAATACACAAGATTT AAGAAAAAAAGAAAAGGAAAATAGAAAGCTACAACTGGAACTTAACCAAGAGAGAGAAAAATTTAACCAA ATGGTGGTGAAACACCAGAAGGAGCTGAATGACATGCAAGCGCAATTGGTAGAAGAATGTACACATAGAA ATGAACTTCAAATGCAGTTGGCTAGCAAAGAGAGTGATATTGAGCAGTTGCGGGCAAAACTTTTGGACCT TTCGGATTCAACTAGTGTTGCTAGTTTTCCTAGTGCAGATGAAACTGATGGAAACCTTCCAGAGTCAAGA ATTGAAGGTTGGCTTTCTGTACCAAATAGAGGAAATATCAAAAGATATGGCTGGAAGAAACAGTATGTTG TGGTAAGCAGCAAAAAGATCTTGTTCTATAATGATGAACAAGATAAGGAACAATCCAGTCCATCTATGGT GTTAGACATAGATAAATTGTTTCATGTCCGACCTGTAACCCAAGGAGATGTGTACAGAGCAGAAACTGAA GAAATTCCTAAAATATTCCAGATACTATATGCAAATGAAGGTGAATGTAGAAAAGATATAGAAGTGGAAC CGGTTCAACAAGGTGAAAAAACTAATTTCCAGAATCACAAAGGCCATGAATTCATTCCTACCCTCTACCA CTTTCCTGCCAACTGTGAGGCCTGTGCCAAACCTTTGTGGCATGTCTTTAAGCCACCACCTGCCCTAGAG TGTCGAAGATGCCATGTTAAGTGCCACAGAGACCATCTAGACAAGAAAGAGGACTTGATTTCCCCGTGCA AAGTAAGTTACGATGTAACATCAGCAAGAGATATGCTGCTGTTAGCATGTTCTCAGGATGAACAAAAAAA GTGGGTTACTCATCTAGTAAAGAAGATTCCTAAGAACCCACCATCTGGCTTTGTCCGTGCCTCTCCTCGA ACGCTGTCAACAAGATCCACTGCAAATCAGTCTTTCCGCAAAGTAGTCAAAAATACATCTGGAAAAACTA GT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224143 representing NM_009071
Red=Cloning site Green=Tags(s) MSTGDSFETRFEKIDNLLRDPKSEVNSDCLLDGLDALVYDLDFPALRKNKNIDNFLSRYKDTINKIRDLR MKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLF YAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKSG HLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYA DSLVGTYSKIMNHKNSLTFPDDNDISKEAKNLICAFLTDREVRLGRNGVEEIKRHLFFKNDQWAWETLRD TVAPVVPDLSSDIDTSNFDDLEEDKGDEETFPIPKAFVGNQLPFVGFTYYSNRRYLPSANASENRSSSNV DKSLQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNLKLDKIMKELDEEGNQRRNLESAVSQIEKEKML LQHRINEYQRKVEQENEKRRNIENEVSTLKDQLEDLRKASQTSQLANEKLTQLQKQLEEANDLLRTESDT AVRLRKSHTEMSKSISQLESLNRELQERNRILENSKSQADKDYYQLQAVLEAERRDRGHDSEMIGDLQAR ITSLQEEVKHLKHNLERVEGERKEAQDMLNHSEKEKNNLEIDLNYKLKSIQQRLEQEVNEHKVTKARLTD KHQSIEEAKSVAMCEMEKKLKEEREAREKAENRVVETEKQCSMLDVDLKQSQQKLEHLTENKERMEDEVK NLALQLEQESNKRLLLQNELKTQAFEADNLKGLEKQMKQEINTLLEAKRLLEFELAQLTKQYRGNEGQMR ELQDQLEAEQYFSTLYKTQVKELKEEIEEKNRENLRKIQELQSEKETLSTQLDLAETKAESEQLARGILE EQYFELTQESKKAASRNRQEITDKDHTVSRLEETNSVLTKDIEMLRKENEELNERMRTAEEEYKLKKEEE INNLKAAFEKNISTERTLKTQAVNKLAEIMNRKDFKIDRKKANTQDLRKKEKENRKLQLELNQEREKFNQ MVVKHQKELNDMQAQLVEECTHRNELQMQLASKESDIEQLRAKLLDLSDSTSVASFPSADETDGNLPESR IEGWLSVPNRGNIKRYGWKKQYVVVSSKKILFYNDEQDKEQSSPSMVLDIDKLFHVRPVTQGDVYRAETE EIPKIFQILYANEGECRKDIEVEPVQQGEKTNFQNHKGHEFIPTLYHFPANCEACAKPLWHVFKPPPALE CRRCHVKCHRDHLDKKEDLISPCKVSYDVTSARDMLLLACSQDEQKKWVTHLVKKIPKNPPSGFVRASPR TLSTRSTANQSFRKVVKNTSGKTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009071 |
| ORF Size | 4062 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009071.2, NP_033097.1 |
| RefSeq Size | 6187 bp |
| RefSeq ORF | 4065 bp |
| Locus ID | 19877 |
| UniProt ID | P70335 |
| MW | 158.6 kDa |
| Gene Summary | Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. Promotes keratinocyte terminal differentiation (By similarity). Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224322 | Rock1 (untagged) - Mouse Rho-associated coiled-coil containing protein kinase 1 (Rock1), (10ug) |
CNY 17,390.00 |
|
| MG224143 | Rock1 (tGFP-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 1 (Rock1), (10ug) |
CNY 11,880.00 |
|
| MR224143L3 | Lenti ORF clone of Rock1 (Myc-DDK-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 1 (Rock1) |
CNY 12,730.00 |
|
| MR224143L4 | Lenti ORF clone of Rock1 (mGFP-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 1 (Rock1) |
CNY 12,730.00 |
