United States
Germany
Japan
United Kingdom
China
Rock1 (NM_009071) Mouse Untagged Clone
CAT＃: MC224322
Rock1 (untagged) - Mouse Rho-associated coiled-coil containing protein kinase 1 (Rock1), (10ug)
CNY 17,390.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1110055K06Rik; Rock-I |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224322 representing NM_009071
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGACTGGGGACAGTTTTGAGACTCGGTTTGAAAAAATCGACAACCTGCTGCGGGATCCCAAATCGG AAGTGAATTCAGATTGTTTGCTGGATGGATTGGATGCCCTGGTTTATGATTTGGATTTTCCTGCCTTAAG AAAAAACAAAAATATTGACAACTTTCTAAGCAGATATAAAGACACAATAAATAAAATTAGAGATTTACGG ATGAAAGCAGAAGATTATGAAGTGGTAAAGGTAATCGGCAGAGGTGCATTTGGAGAAGTTCAATTGGTGA GGCATAAATCCACTAGGAAGGTTTATGCTATGAAGCTGCTCAGCAAATTTGAAATGATTAAGAGATCGGA TTCTGCTTTTTTCTGGGAAGAAAGGGACATCATGGCGTTTGCCAATAGTCCTTGGGTTGTTCAGCTTTTT TATGCATTCCAAGATGACCGGTATCTCTACATGGTGATGGAGTACATGCCTGGCGGAGACCTTGTCAACC TGATGAGCAACTATGACGTGCCTGAAAAATGGGCAAGATTCTATACTGCAGAAGTAGTTCTTGCATTGGA TGCAATCCATTCCATGGGTTTTATTCATAGAGATGTAAAGCCTGATAACATGCTGCTGGATAAGTCTGGA CATTTGAAGTTAGCAGATTTTGGTACTTGTATGAAGATGAATAAGGAAGGTATGGTACGATGTGACACAG CAGTTGGAACACCTGATTATATCTCACCCGAAGTATTAAAATCCCAAGGCGGTGATGGCTATTATGGACG AGAGTGTGACTGGTGGTCAGTTGGGGTATTTCTATATGAAATGCTCGTAGGTGACACACCTTTCTATGCA GATTCTTTGGTTGGGACTTACAGTAAAATTATGAACCATAAAAATTCACTTACTTTCCCTGATGATAATG ACATATCAAAAGAGGCAAAAAATCTCATTTGTGCCTTCCTTACAGACAGAGAAGTGAGATTGGGCAGAAA TGGTGTAGAGGAAATCAAACGTCATCTCTTCTTCAAAAATGATCAGTGGGCTTGGGAAACGCTCCGAGAC ACTGTAGCACCAGTTGTGCCTGATTTAAGTAGTGACATTGATACTAGTAATTTTGATGACTTAGAAGAAG ATAAAGGAGATGAAGAAACATTTCCTATTCCAAAAGCTTTTGTTGGCAATCAGCTACCTTTTGTAGGATT TACATATTATAGCAATCGTAGATACTTACCTTCAGCAAATGCCAGTGAGAACAGATCTAGCTCCAATGTG GATAAAAGCTTGCAGGAAAGTTTACAGAAAACAATCTACAAGCTTGAAGAGCAACTGCATAATGAAATGC AATTAAAAGATGAAATGGAGCAGAAATGCAGAACTTCAAACTTAAAACTAGACAAGATAATGAAAGAATT GGATGAAGAGGGGAATCAAAGAAGAAATCTAGAATCTGCAGTGTCTCAAATTGAGAAAGAAAAGATGTTG CTACAACATAGAATTAATGAGTACCAAAGAAAAGTGGAACAGGAAAATGAGAAGAGAAGAAATATAGAAA ATGAAGTTTCTACACTAAAGGACCAGTTAGAAGACTTACGGAAAGCAAGTCAGACCTCACAGCTTGCTAA TGAGAAGCTGACACAGTTACAGAAGCAGTTAGAAGAAGCGAATGACTTACTTAGGACAGAATCAGACACA GCTGTAAGATTAAGGAAGAGTCACACAGAGATGAGCAAGTCAATTAGTCAGCTAGAGTCCCTGAACAGAG AATTACAGGAAAGAAACAGAATTTTAGAGAATTCCAAGTCACAAGCAGACAAGGATTACTACCAGCTGCA AGCTGTATTGGAAGCTGAGCGAAGAGACAGAGGGCATGATTCTGAGATGATTGGAGACCTTCAAGCACGA ATTACATCTTTACAAGAAGAAGTGAAACATCTCAAACATAATCTTGAAAGAGTGGAAGGAGAAAGAAAAG AGGCTCAAGACATGCTTAATCATTCAGAAAAGGAAAAGAATAATTTGGAGATAGATTTGAATTACAAGCT CAAGTCTATACAACAGCGGTTAGAACAAGAAGTAAATGAACATAAAGTCACCAAAGCACGCCTAACTGAC AAGCACCAATCTATCGAAGAGGCAAAGTCTGTTGCCATGTGTGAAATGGAAAAAAAGCTGAAGGAAGAGA GAGAAGCTCGAGAGAAGGCAGAAAATAGAGTTGTTGAGACTGAGAAACAATGTTCCATGCTAGATGTTGA CCTGAAACAGTCTCAGCAGAAGCTAGAACATTTGACCGAAAATAAAGAAAGGATGGAGGATGAAGTTAAG AATCTCGCCCTGCAGCTGGAACAGGAATCAAATAAACGGCTCTTGTTACAGAATGAATTGAAGACTCAAG CATTTGAAGCAGACAATTTAAAAGGTTTAGAAAAACAGATGAAACAGGAAATAAATACTTTATTGGAAGC AAAGAGATTGTTAGAATTTGAGTTAGCTCAGCTTACAAAGCAATATAGGGGAAATGAAGGGCAAATGCGG GAGTTACAAGATCAACTTGAAGCTGAGCAGTATTTCTCTACACTTTATAAAACCCAAGTAAAGGAGCTTA AAGAAGAAATTGAAGAAAAAAACCGAGAAAATTTAAGGAAAATACAGGAACTGCAAAGTGAAAAAGAAAC TCTCTCTACTCAGTTGGATTTAGCAGAAACAAAAGCTGAGTCTGAGCAGCTGGCGCGAGGGATTCTGGAA GAACAGTATTTTGAGTTGACTCAGGAAAGCAAGAAAGCTGCTTCAAGAAATAGACAAGAGATTACAGATA AAGATCATACTGTTAGTCGGCTTGAAGAAACAAACAGTGTGCTAACCAAAGATATTGAAATGCTAAGAAA AGAAAATGAAGAGCTAAATGAAAGAATGAGGACAGCAGAAGAAGAATATAAATTGAAGAAGGAGGAAGAA ATAAATAATCTTAAGGCTGCTTTTGAAAAGAATATCAGTACCGAACGGACCCTTAAAACACAGGCTGTTA ACAAGTTGGCAGAAATAATGAATCGCAAAGATTTTAAAATTGATCGAAAGAAAGCTAATACACAAGATTT AAGAAAAAAAGAAAAGGAAAATAGAAAGCTACAACTGGAACTTAACCAAGAGAGAGAAAAATTTAACCAA ATGGTGGTGAAACACCAGAAGGAGCTGAATGACATGCAAGCGCAATTGGTAGAAGAATGTACACATAGAA ATGAACTTCAAATGCAGTTGGCTAGCAAAGAGAGTGATATTGAGCAGTTGCGGGCAAAACTTTTGGACCT TTCGGATTCAACTAGTGTTGCTAGTTTTCCTAGTGCAGATGAAACTGATGGAAACCTTCCAGAGTCAAGA ATTGAAGGTTGGCTTTCTGTACCAAATAGAGGAAATATCAAAAGATATGGCTGGAAGAAACAGTATGTTG TGGTAAGCAGCAAAAAGATCTTGTTCTATAATGATGAACAAGATAAGGAACAATCCAGTCCATCTATGGT GTTAGACATAGATAAATTGTTTCATGTCCGACCTGTAACCCAAGGAGATGTGTACAGAGCAGAAACTGAA GAAATTCCTAAAATATTCCAGATACTATATGCAAATGAAGGTGAATGTAGAAAAGATATAGAAGTGGAAC CGGTTCAACAAGGTGAAAAAACTAATTTCCAGAATCACAAAGGCCATGAATTCATTCCTACCCTCTACCA CTTTCCTGCCAACTGTGAGGCCTGTGCCAAACCTTTGTGGCATGTCTTTAAGCCACCACCTGCCCTAGAG TGTCGAAGATGCCATGTTAAGTGCCACAGAGACCATCTAGACAAGAAAGAGGACTTGATTTCCCCGTGCA AAGTAAGTTACGATGTAACATCAGCAAGAGATATGCTGCTGTTAGCATGTTCTCAGGATGAACAAAAAAA GTGGGTTACTCATCTAGTAAAGAAGATTCCTAAGAACCCACCATCTGGCTTTGTCCGTGCCTCTCCTCGA ACGCTGTCAACAAGATCCACTGCAAATCAGTCTTTCCGCAAAGTAGTCAAAAATACATCTGGAAAAACTA GTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_009071 |
| Insert Size | 4065 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_009071.2, NP_033097.1 |
| RefSeq Size | 6187 bp |
| RefSeq ORF | 4065 bp |
| Locus ID | 19877 |
| UniProt ID | P70335 |
| Gene Summary | Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. Promotes keratinocyte terminal differentiation (By similarity). Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG224143 | Rock1 (tGFP-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 1 (Rock1), (10ug) |
CNY 11,880.00 |
|
| MR224143 | Rock1 (Myc-DDK-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 1 (Rock1) |
CNY 9,264.00 |
|
| MR224143L3 | Lenti ORF clone of Rock1 (Myc-DDK-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 1 (Rock1) |
CNY 12,730.00 |
|
| MR224143L4 | Lenti ORF clone of Rock1 (mGFP-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 1 (Rock1) |
CNY 12,730.00 |
