Synm (NM_201639) Mouse Tagged ORF Clone
CAT#: MR224051
- TrueORF®
Synm (Myc-DDK-tagged) - Mouse synemin, intermediate filament protein (Synm), transcript variant 1
ORF Plasmid: tGFP
"NM_201639" in other vectors (4)
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CNY 15,580.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 4930412K21Rik; AI852401; Dmn; E130104F11; Syn; Synemin |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR224051 representing NM_201639
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTCCTGGCGGCTGCAGACGGGCTCTGAAAAGGCGGAGCTGCAGGAGCTCAACGCCCGGCTTTACG ACTACGTGTGCCGGGTGCGAGAGCTGGAGCGCGAGAACCTGCTACTGGAGGAGGAGTTGCGCAGCCGGCT GAGCCGGGAGGACCGCTGGGCTGAGGATCAGGCACTCTACGCCGAGGAGGCGCGCAGCCTACGACAGCAG CTAGATGAACTGAACTGGTCCACAGCCCTGGCCGAGGGCGAGCGCGACGCGCTGCGGAGGGAACTGCTGG AGCTGCAGCGGGAGGGTGTGGAAGCCGGCACTGCCCGCAGCCGCCTGGATGCCGAGCTGGGAGCGCAACG GCGGGAGCTAGAGGAGGCTCTGGGCGCGCGCGCCGCCCTCGAGGCGCTGCTGGGCCGGCTGGAAACTGAG CGCCGCGACTTGGACGCTGCCCACGAACGCCAAGTGCGAGATCTACGAGCCCGCGCTGCCAGCCTCACCA TGCACTTCCGAGCCCGCGCCACCAGCCCCGCCGCGCCCCCGCCGCGCCTGCGGGACGTGCACGACAGCTA CGCCCTGCTGGTGGCCGAGTCGTGGAGGGAGAGCGTGCAGCTGTACGAGGACGAGGTGCGCGAGCTGGAG CAGGCGCTGCGCCGCGGCCAAGAGAGCCGGCTCCAGGCGGAGGACGAGGCGCGGCTGTGCGCGCAGGAGG CAGACGCGCTGCGGAACCAGGCGCTGGAGTTGGAGCAGCTGCGCGCGAGGCTGGAGGATGAGCTGCTGCG GATGCGCGAGGAGTACGGGATGCAGGCGGAGGAGCGGCAGAGAGTGATTGACAGCCTGGAGGATGAGAAG GAAGCCCTTACCTTGGCCATGGCTGACCGGCTGCGGGATTACCAGGAGCTCCTGCAGGTGAAGACTGGAC TCAGCTTGGAGGTAGCCACCTACAGGGCCTTGTTGGAAGGAGAAAGCAATCCGGAGATATTGATCTGGAC TGAGAACATTGAAAACGTGCCACAAGAGCCCAGAAACACATCCTATCGCTACACCAACTCAGTATTGCAG AGGAAAAATGAGAAAAATCTCTTTCCAAGGCGAAAAACACCTTGGGCAGCTGTAAATCACAGCTCGGCCT CGTATTCTAACTGGCCAGGGCACCTTGACTCACAGACAACCACAGCTGTAGGAAGTGCTGCCAGAAGGGG TCTCCTGACCTCCCGCCATTCTTCCTCAGCTACAACCTCAGGGCAGCAAAAGCCCCTGGAGAAAACCATC AGCAGCCGAGCCAATTTAAGACCGGTCACGCCAACCCACGGCTTTCTAAGAAACACTGATGCTCAAATGA AAACACTCCCCCATAGATCCAAAGTCGAAGGCACGGGGGACACCCACGCACGGCGGGCTACAGAGTCTGT TATTACCAGAGAGTCATACAGAGGTCACCAGGGCCATGTGGCAGCAGGTGCCGTGAGCAGCACTCCGTCA AATGAGAGGACTGTCATTCTGGGAAAGAAATTAGAAGCGCAAGCCACTAAAGAACAAGAAAGGGACAGAT CAGGGGTCATCAGAATAAAGCCAGAAGAGAAAATGTTTGATTCTAAAGAGAAGGCTTCAGAGGAAAGAAA CTTGAGGTGGGAAGAGCTAACCAAGTTAGATAGAGATGCCAGAAAGAGAGAAAGCCGGCATTTGAGGGAT GAGGCCAGAGAGAAGGAAGCACTGAAGGAGAGAAGTGTAAAAGAAAGAGAGGTGCCCATCAGTCTAGAAG TATCCCGGGGCAGCAGAGCAGAGGTGTCCACCATACACTTGCAGTCACCTGGAAGGAAGGACGTTAGCCA CAGTGGGGGAAGAGAGGCAGAGACCAAAGAGACAAGGTTCCGGCTGGATACCCAGGACACTGCCAGCTCT CTGCAAAGTGACTCAACGACAGAAACCATAGCCGAAAGCATTGTCACCACCATCCTTAAGCAGTTTACCC AGTCTCCAGGTGCCGAGGAGGAGGCCACTTCTTTCCCGGACACAAAGGTCACTTATGTGGACAGGAAAGA GTTTCCTGGTGATGGGAAAACCAAGACAGAAATCGTTGTGGAGTCGAAGCTGACAGATGTTGTTGATGTT TCTGATGAAGCAGGCCTGGACTACCTTCTAAGCAAGGATGTCAAGGAAGTAGGACTGAAGGGGAAGTCAA CGGAGACGATGATCGGAGAGATGATCAACCTGGGTCTGAAAGGCAGGGAGGGGAGAGCTAAAGTCGTAAA TGTGGAGATTGTGGAAGAGCCCATGAGCTACATAGGTGGTGGGAAAATAGACTTTTCTACCCCCTTTCAG GTAGAAGAAGTCGACGACGTGTCTCCCAGCCCCAAGGGCTTTGTGGAGGAAGAGGATGGTGAAGGAGAGA CCCACATGGCATTCTCAATGCGTCCACATCAGACCAAACAGCCCCAGGGGACCATCCCTCACGTGGAAGA AGTGACGGAGGCGGGCGACTCAGAGGGGGAGCAGAGCTATTTTGTGTCTACCCCAGATGAGTACCCCGGG GGGCATGACAGAGAAGATGATGGCTCAGTGTATGGGCAGATTCATATCGAGGAAGAGTCTACCATCCGGT ACTCATGGCAAGACGAAATTGCGCAGGGGACCTGGAGAAGGAAAATGAGAGGTGACGTGGGGGGAGAGAA GCCTGTGAAGGTCCTGGAGGTCCCAGCACTTTCCCTGGGTGGTGCTATTGGTTCTGCTCACTTGAAGGAA GAAGCCAGTGGTGAACTCCGTGCTGAACCCACAGTCATTGAGAAAGAGATCAAAATACCGCATGAATTCC ACACCTCCATCAAGGGTGTCTTCTCCAGTGAGCCCCGGCACCAGCTGGTGGAGGTCATCGGGCAGCTGGA GGAAACATTGCCCGAGCGCATGAAGGAGGAGCTGTCTGCCCTGACCAGACAGAGTCAAGGAGAGTCAGGG AGCGTTTCTGTGGACGTAAAGAAAGTCCAGAGCGCTGCTGGTGGTTCTGTGACTTTGATGGCTGAGGTCA ACCTCTCACAGACTGTAGATGCTGATCAGTTGGACCTGGAGCAGTTGAGCAGAGATGAAGCTGGGGAAAT AGAGAGGGCTGTGGAGTCTGTGGTCAGAGAGAGCTTGGCCAAGCGCTCCAGCCCAGTGCCTAGAAGCCCA GACAGGGAAGATGGAGAGGAGGTGCCTGCTGGTGGCATTCTCTTCAAGCGCTGGGCCACCAGGGAGCTGT ATAGCCCATCTGGTGAGAGGGATGATGCTGGCCAGGTCTCTCCCAGCTCAGATCAGCGTGTTACCCAGGG CCCAGTGTCAGCTACTGTGGAAGTGACCAGCCCAACGGGTTTTGTACAGTCTCATGTGCTGGAGGATGTG AGCCAGTCTGTAAGGCATGTTAAACTAGGTCCCACTGAAATGTGGAGGACAGAACAGGTGACCTTCGGAG GACCCACCGCACAGGTGGTGGAGGTAAGTGGAGACTTCAGCGAGGCAGTCAGCTCTGAGGGAGCCAGCCG CTCTGTGAGGCACATTACACTGGGTCCCCATCAAAGCCAAGTGTCCACAGAAGTCATTTTCCGAGGCTCT GTGCCTACCTGGCAGGAAACAGGAGACACAGAAAAGCCTGGCCCAGTGGTACTGTCTGTGGGAGCTGACA TCTCAGGGAGCGGCAGGATGCCTGGCTCTGAGCGGTCTCACACTGAGAAAGAAATTCGTTTTCAGGGTCC TGTTTCTGGGACAGCCCAGGTTGGTGGTAACTTTGCAACTGAAGAGTCAGTGGGTAGCCAGACTTTTGTA AGGAGTCTCCAGTTAGGCCCCAAAGAAGGGTTCAGAGAGGAAATCCAGTTCATAGCTCCTATCCCAGACA AGGTAGGGTGGGGAGAAGAGGATTCTGAGCACACCAAAGTGTCGCTAGAGAGGGCTACATCCATCCAGCG TATTGACATTGTGCCCCAGAGGTACCTAGCTTCCAAGCAGATGGCCCCACAGACATTGGAATTCAGGGAC TCAGAAGACATGGTTATGGTGGAAGGTTCAGCAGGCACAATCCAGGCCACTCACAATTTCACCTCAGATA GAGAAATCCTCCAGAACAAAGAGAACACATTCCAGAGGGTCATTTCTGGGTCTCCTCCAGACAGTGTGGG CGACACGGGAGCAGAGGTGACAGCAAACGTCAGCAGGTCCTTTCGGCACATTCAAATAGGTCCTACAGAG GAAGAACCCTCTGAATACTTTGTCACCGGGAGACCCGTATCTAAAACATTTGTACTTGATGGTTCCGTGG CGTCCCCTGGGCTGGTTGGGGGAGCAGACGGTGGTAGCACACCGTGTCGGATTGCACTGGGGCCCAAAGA AACTTCATTTACTTTCCAGATGGATCTGAGCGACACGAGAGCGATCCGCAGCTGGACCCGAGACACAGGG TCGGAAGTGGAAGCTCACGGCGTGTCTCACCGTGGTGGCTGGAGAATTGCACACAGTAGGGATGAGCGAG TGGCCAGCACTGGCTCTGGGGCCTCCCCTGGGGATGCACACCAGGCCCCTGGAGAGAAAGGCACGGAGCA GGCTGGATTTGACAAGACGGTACAGTTGCAGAGAATGGTAGACCAAAGGTCGGTGGCCTCAGATGAGAAG AAAGTGGCTCTTCTTTATCTAGACAATGAAGAGGAGGAGGAGGAGGAGGGCGAAGGGTGGTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224051 representing NM_201639
Red=Cloning site Green=Tags(s) MLSWRLQTGSEKAELQELNARLYDYVCRVRELERENLLLEEELRSRLSREDRWAEDQALYAEEARSLRQQ LDELNWSTALAEGERDALRRELLELQREGVEAGTARSRLDAELGAQRRELEEALGARAALEALLGRLETE RRDLDAAHERQVRDLRARAASLTMHFRARATSPAAPPPRLRDVHDSYALLVAESWRESVQLYEDEVRELE QALRRGQESRLQAEDEARLCAQEADALRNQALELEQLRARLEDELLRMREEYGMQAEERQRVIDSLEDEK EALTLAMADRLRDYQELLQVKTGLSLEVATYRALLEGESNPEILIWTENIENVPQEPRNTSYRYTNSVLQ RKNEKNLFPRRKTPWAAVNHSSASYSNWPGHLDSQTTTAVGSAARRGLLTSRHSSSATTSGQQKPLEKTI SSRANLRPVTPTHGFLRNTDAQMKTLPHRSKVEGTGDTHARRATESVITRESYRGHQGHVAAGAVSSTPS NERTVILGKKLEAQATKEQERDRSGVIRIKPEEKMFDSKEKASEERNLRWEELTKLDRDARKRESRHLRD EAREKEALKERSVKEREVPISLEVSRGSRAEVSTIHLQSPGRKDVSHSGGREAETKETRFRLDTQDTASS LQSDSTTETIAESIVTTILKQFTQSPGAEEEATSFPDTKVTYVDRKEFPGDGKTKTEIVVESKLTDVVDV SDEAGLDYLLSKDVKEVGLKGKSTETMIGEMINLGLKGREGRAKVVNVEIVEEPMSYIGGGKIDFSTPFQ VEEVDDVSPSPKGFVEEEDGEGETHMAFSMRPHQTKQPQGTIPHVEEVTEAGDSEGEQSYFVSTPDEYPG GHDREDDGSVYGQIHIEEESTIRYSWQDEIAQGTWRRKMRGDVGGEKPVKVLEVPALSLGGAIGSAHLKE EASGELRAEPTVIEKEIKIPHEFHTSIKGVFSSEPRHQLVEVIGQLEETLPERMKEELSALTRQSQGESG SVSVDVKKVQSAAGGSVTLMAEVNLSQTVDADQLDLEQLSRDEAGEIERAVESVVRESLAKRSSPVPRSP DREDGEEVPAGGILFKRWATRELYSPSGERDDAGQVSPSSDQRVTQGPVSATVEVTSPTGFVQSHVLEDV SQSVRHVKLGPTEMWRTEQVTFGGPTAQVVEVSGDFSEAVSSEGASRSVRHITLGPHQSQVSTEVIFRGS VPTWQETGDTEKPGPVVLSVGADISGSGRMPGSERSHTEKEIRFQGPVSGTAQVGGNFATEESVGSQTFV RSLQLGPKEGFREEIQFIAPIPDKVGWGEEDSEHTKVSLERATSIQRIDIVPQRYLASKQMAPQTLEFRD SEDMVMVEGSAGTIQATHNFTSDREILQNKENTFQRVISGSPPDSVGDTGAEVTANVSRSFRHIQIGPTE EEPSEYFVTGRPVSKTFVLDGSVASPGLVGGADGGSTPCRIALGPKETSFTFQMDLSDTRAIRSWTRDTG SEVEAHGVSHRGGWRIAHSRDERVASTGSGASPGDAHQAPGEKGTEQAGFDKTVQLQRMVDQRSVASDEK KVALLYLDNEEEEEEEGEGWF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_201639 |
ORF Size | 4683 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_201639.2, NP_964001.2 |
RefSeq Size | 7866 bp |
RefSeq ORF | 4686 bp |
Locus ID | 233335 |
UniProt ID | Q70IV5 |
MW | 173.7 kDa |
Gene Summary | Type-VI intermediate filament (IF) which plays an important cytoskeletal role within the muscle cell cytoskeleton. It forms heteropolymeric IFs with desmin and/or vimentin, and via its interaction with cytoskeletal proteins alpha-dystrobrevin, dystrophin, talin-1, utrophin and vinculin, is able to link these heteropolymeric IFs to adherens-type junctions, such as to the costameres, neuromuscular junctions, and myotendinous junctions within striated muscle cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC224687 | Synm (untagged) - Mouse synemin, intermediate filament protein (Synm), transcript variant 1, (10ug) |
CNY 20,050.00 |
|
MG224051 | Synm (tGFP-tagged) - Mouse synemin intermediate filament protein (Synm) transcript variant 1, (10ug) |
CNY 17,100.00 |
|
MR224051L3 | Lenti ORF clone of Synm (Myc-DDK-tagged) - Mouse synemin, intermediate filament protein (Synm), transcript variant 1 |
CNY 17,480.00 |
|
MR224051L4 | Lenti ORF clone of Synm (mGFP-tagged) - Mouse synemin, intermediate filament protein (Synm), transcript variant 1 |
CNY 17,480.00 |