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Grhl1 (NM_145890) Mouse Tagged ORF Clone
CAT＃: MR223939
- TrueORF®
Grhl1 (Myc-DDK-tagged) - Mouse grainyhead-like 1 (Drosophila) (Grhl1), transcript variant 2
ORF Plasmid: tGFP
"NM_145890" in other vectors (4)
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CNY 5,320.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | LBP-32; MGR; Tcfcp2l2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR223939 representing NM_145890
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTCTCCTTGATGATGAACTGTGTGACCTAAACAGCATACCAAATGTGACGGAGCAGCCCCTCATTT CTGCTGGAGAAAACAGGGTGCAAGTGCTGAAAAACGTGCCCTTCAACATCGTCCTCCCCCATAGCAACCA GCTGGGCATTGATAAGAGAGGCCATCTGACAGCTCCCGATACAACAGTCACTGTCTCCATAGCAACCATG CCTACCCACTCCATCAAGACAGAAATCCAGCCGCACGGCTTTGCTGTGGGAATCCCACCAGCCGTGTACC ACTCTGAGCCCACCGAACGTGTGGTGGTTTTTGACCGGAGCCTCAGCACTGATCAGTTCAGCTCTGGCAC TCAGCCCCCCAATGCTCAGCGGAGGACTCCAGACTCCACCTTCTCCGAGACCTTCAAGGAGGGCGTTCAG GAGGTTTTCTTCCCCTCGGAACTCAGCCTTCGGATGCCGGGCATGAATTCAGAGGACTATGTCTTTGACA ATGTTTCTGGGAACAACTTTGAGTATACCCTGGAAGCCTCCAAGTCACTGCGGCAGAAGCAAGGGGACAG CACTATGACATACCTGAATAAAGGCCAGTTCTATCCTGTCACCTTAAAGGAAGGAAGCAGCAATGAAGGG ATTCACCACCCTATCAGCAAAGTTCGAAGTGTGATCATGGTGGTTTTTGCTGAAGACAAAAGCAGAGAAG ACCAGCTGAGACACTGGAAGTACTGGCACTCCCGTCAGCACACGGCCAAACAGAGGTGCATTGACATTGC TGACTACAAAGAAAGCTTCAACACTATCAGCAACATTGAGGAGATAGCTTATAACGCCATTTCCTTCACG TGGGACATCAATGATGAGGCAAAGGTCTTCATCTCTGTGAACTGCTTGAGCACAGATTTCTCTTCTCAGA AGGGTGTGAAGGGCTTGCCACTCAACATTCAAATCGACACATACAGCTATAACAACCGCAGCAACAAGCC GGTTCACCGGGCCTACTGCCAGATAAAGGTCTTCTGCGACAAGGGAGCTGAAAGGAAAATTCGGGATGAA GAACGAAAACAGAGCAAGAGAAAAGTGTCTGACGTTAAAGTGCAGCTGCTTCCCTCGCACAAACGGACAG ACATCACAGTGTTCAAGCCCTTCCTGGACCTCGACACTCAGCCTGTCCTCTTCATTCCGGACGTGCATTT TACCAACCTGCAGCGGGGCAGTCATGTTCTTTCCCTCCCCTCTGAAGAACTGGAAGGTGAAGGCTCTGTC TTGAAAAGAGGGCCATTCGGAACCGAAGATGACTTTGGAGTTCCTCCTCCTGCTAAGCTGACTCGGACAG AAGAACCCAAGAGAGTGCTGCTCTATGTCCGAAAGGAATCAGAAGAAGTCTTCGACGCCCTGATGCTCAA GACGCCGTCTTTGAAGGGCCTGATGGAGGCAATTTCAGACAAGTATGATGTCCCCCATGACAAGATTGGG AAAATATTTAAGAAGTGCAAAAAAGGGATCCTCGTGAACATGGACGACAACATTGTGAAGCACTACTCCA ATGAGGACACCTTCCAGCTGCAGATAGAGGAAGCCGGCGGCTCGTACAAGCTCACCCTGACAGAGATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223939 representing NM_145890
Red=Cloning site Green=Tags(s) MVLLDDELCDLNSIPNVTEQPLISAGENRVQVLKNVPFNIVLPHSNQLGIDKRGHLTAPDTTVTVSIATM PTHSIKTEIQPHGFAVGIPPAVYHSEPTERVVVFDRSLSTDQFSSGTQPPNAQRRTPDSTFSETFKEGVQ EVFFPSELSLRMPGMNSEDYVFDNVSGNNFEYTLEASKSLRQKQGDSTMTYLNKGQFYPVTLKEGSSNEG IHHPISKVRSVIMVVFAEDKSREDQLRHWKYWHSRQHTAKQRCIDIADYKESFNTISNIEEIAYNAISFT WDINDEAKVFISVNCLSTDFSSQKGVKGLPLNIQIDTYSYNNRSNKPVHRAYCQIKVFCDKGAERKIRDE ERKQSKRKVSDVKVQLLPSHKRTDITVFKPFLDLDTQPVLFIPDVHFTNLQRGSHVLSLPSEELEGEGSV LKRGPFGTEDDFGVPPPAKLTRTEEPKRVLLYVRKESEEVFDALMLKTPSLKGLMEAISDKYDVPHDKIG KIFKKCKKGILVNMDDNIVKHYSNEDTFQLQIEEAGGSYKLTLTEI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145890 |
| ORF Size | 1608 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145890.2, NP_665897.2 |
| RefSeq Size | 3100 bp |
| RefSeq ORF | 1611 bp |
| Locus ID | 195733 |
| MW | 61.2 kDa |
| Gene Summary | Transcription factor involved in epithelial development. Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' (PubMed:18288204, PubMed:21081122). Important regulator of DSG1 in the context of hair anchorage and epidermal differentiation, participates in the maintenance of the skin barrier (PubMed:18288204, PubMed:24586629). There is no genetic interaction with GRHL3 no genetic interaction with GRHL3, no functional cooperativity due to diverse target gene selectivity during epithelia development (PubMed:21081122).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217636 | Grhl1 (untagged) - Mouse grainyhead-like 1 (Drosophila) (Grhl1), transcript variant 2, (10ug) |
CNY 6,840.00 |
|
| MG223939 | Grhl1 (tGFP-tagged) - Mouse grainyhead-like 1 (Drosophila) (Grhl1) transcript variant 2, (10ug) |
CNY 5,890.00 |
|
| MR223939L3 | Lenti ORF clone of Grhl1 (Myc-DDK-tagged) - Mouse grainyhead-like 1 (Drosophila) (Grhl1), transcript variant 2 |
CNY 7,220.00 |
|
| MR223939L4 | Lenti ORF clone of Grhl1 (mGFP-tagged) - Mouse grainyhead-like 1 (Drosophila) (Grhl1), transcript variant 2 |
CNY 7,220.00 |
