Mia3 (NM_177389) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	9130229H14Rik; A930039G15Rik; AU022327; B230399H06Rik; C80126; Gm1525; Tango; TANGO1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR223502 representing NM_177389, codon optimized. Due to the complexity of NM_177389, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCGCCCCGGGGCTCTTGTTCTGGCTGTTCGTCCTGGGAGCCCTGTGGTGGGTTCCGGGTCAGA GCGACCTGAGTCACGGCAGGCGGTTTTCAGATCTCAAAGTGTGTGGGGACGAAGAGTGCTCTATGCTGAT GTACAGGGGCAAGGCTCTGGAAGATTTCACCGGCCCTGATTGCAGGTTTGTGAACTTTAAAAAAGGCGAT GACGTGTACGTGTACTATAAGCTCGCTGGCGGAAGTCTGGAACTGTGGGCTGGAAGCGTGGAACACTCTT TCGGTTACTTCCCTAAGGACCTCATCAAAGTGCTGCATAAATACACAGAAGAGGAGCTGCATATCCCGGC GGACGAAACCGATTTCGTGTGCTTCGAGGGCGGGCGGGATGACTTCAATAGCTACAATGTTGAGGAGCTT CTTGGGAGCCTTGAACTGGAGGATAGCGTACCCGAGGAATCTAAGAAGGCCGAGGAAGTTTCCCAACACA GAGAGAAATCCCCTGAAGAGAGCCGAGGCCGCGAACTGGATCCAGTGCCAGAGCCGGAGGCCTTCCGAGC TGATTCAGAGGACGGCGAAGGCGCGTTTAGTGAGTCTACAGAGGGACTGCAGGGGCAGCCCTCCGCCCAG GAGAGCCATCCTCATACCTCTGGGCCTGCAGCCAATGCACAGGGGGTGCAGTCAAGTTTGGACACCTTTG AAGAGATCCTGCACGATAAGCTCAAAGTGCCCGGGTCAGAAAGTCGCACAGGCAACTCAAGTCCCGCTAG TGTGGAGCGCGAGAAAACAGATGCCTACAAGGTTCTGAAGACCGAAATGAGCCTCGACCTCAAGACCAAG TTTGGTTCTACTGCCGACGCTCTGGTAAGTGACGATGAGGCAACACGACTGGTCACAAGTTTGGAGGATG GCTTCGACGAGGCCCTCGACGCCGAATACTATCCCATGGAAGAGGAAGAGGAGGTGGAAGAGGACGCTGA CTCCAGCGATGAGCTGCCCCTTTTGACCTTCAGTGATAAGGATGAAAAGGTGCCTGGAAAGCCCATGATC GAGAAATACCTGACAGACAAAGATCCGAACCTGTCCGAAGAAGACAAAGTAGAACCCCCCACCTGGGGAG ATGCCTTTTTTTCTATCGTGACTGGGGGCGAGGGAAAGCCAGGGGTGGTGGATCTCGAGAGATCAATAGA GGAAGAAGAAGATGTTAGCGTGTCATCTAGTCATCAGCGCAAGCCACAGCCCGCAGCTGGCTATACCGAT TCTGAGGATGAAGGCGATGATCTGTTCGTCGAAGAACCCAAGACTAATGATGTTAAGGATAGTGAAACCG ATCCCGAGCTGGTTATCACCGGAGAGGAAAAGGATATTCAGGAGAGCAGGAAGGGCCTGGTCCAACCAGA AAGCCAGTCTGAAGATGCTAAATCAGAGACTGCCTCCGCCTATAGGCTTCAGGGTAGCAAGCTCAACCCG CTGAGCGCTGCTGAGAAGGGCCGGGATTTCACTCTGAAAGCCGTTTTTGAGAAGAAGGAAAATGGCCTGA AGGAGAGTGTGATCCACATTTCAAAGGAAACCTTGCACGAGGACAAGACAAGGGAGATCCAGCGGGATTC CCTCGAGAGCGAACTTGTACACAGGGCTCTCGGCAGTTCAGTGACAGAGAATAATAAGCCAAAAAGCTTG GGGGTCGCCCCACTGCTGGGCAACAATAAGCCAGACGCTAGCAAGGACTCCACAGAGGTTCCAGATGGGT CCGTCAGCGGTCCAAAAGCAGGCCAGCAGGAAGGCTTCCTGGAGCCTGGTCTGAAAACGCAGCATCAACC CAGGTTCTCCCCCCCTGAAGAAACTGGGCCTTCTAGGGAGCTGGGCGGAAAGGTGCCCATATCCGGTCGC AACCTTAGTTGGCAGCAGGAGCAGGACGTGGCTGCAGTAGTTGGGAAGCACGCGAACGAAAAGACTGGTT TCCCAGAGGAGGAGAGCCGCGAAGACGGCACCGATGCTGAACAGGCACGAGCTATCCGCAGACCGCAAGA GGCCGAATCCCCAGAAGTTCTTAGCGTCCAGCCGGGTAGACCTGACGAGGAGGAAGAGGAAGAAGAAGGT GACAATTATCCGCCTGAAGGACTGATGGAGGATGAAAATGCAGTAAGCGCACAGCAGTCAAGAGAAAATT CACCTTCCGCAAGAGACGGGAGGTCAGATATGAACAGTCAGGTCTTTGAAAAGGTGATCCTTGGGACACT CAACCTGAACACCGAGAAAACGAAGCAACCCGCCAACATGATACTTGAGACTGGGCAGGAAAGCGAAACC ACAAGTGAGGAGGCAGGGGATGTCGGAAAGGAGAGCGGGCACTCCGTGGTTGTGGATAGCGAAGAGAGCC ATCTCGCTGATATGAGAGCTCAAAGGCCCTCTCAGGTCCACGGGCTGCGGGATGAGACTGCGGCCCAGAC TCCGGGATCTGGGGAAGCAGTCCTCAGCAAAAATCCTAATGACCTCCAGAAGGATAATCCTGAGGAGGAA CTCGTGAATACACTTGGGCTTGAGGATCCAGGAGTAGGTGAGATAAGCGAGGGAGAACCAGAAGATACCA AGGAATTTGGCGTGAGTGAATCCCAGGGGACTGATGCCGAGGACCTCCGCGATGATCCCAGCCGGCAGGC CACCCCAGAGATCCCCGATATTGTACTGAAAAGTATTCGCGAAGATCTGCCAATCATCAACTCCTTCTTT AAGGATGATCAGCAGAGCCTGCACCGCTTTTTGAAATACTTTGACGTAAGGGAACTTGAAGGTCTGCTCG AAGATATGTCAATTCGGCTTCGCAGTGCTCATCAAAACTCCCTTCCTTACAATATGGAAAAAGTGCTGGA CAAGGTGTTCCGCGCAAGTGAAAGTAGAATACTTTCCATGGCAGAGAAGATGCTCGATACTGGGGTGGCC AAAAATCGGGATCTGGGGTCAAAAGAAAGTAGCCCTCTCGAGGAGGCAGAAGTCTTGGATGATATCCAGG ACCTTATTTACTTTGTTAGGTACCAGTACAGCGGTGTGGAAACCGCCCCCCTCGTAACCCCTCCTCCCCC GGAGGAAGGATGGGCACGACCTGGAGAGGAACGACAACCACCTCAGCAGGACAGTCTTCCCCAAGAGAAT ACAGGCGATTTGTCTGTCCAACCACCTGAGGAGCCAGAGCTGTCCGATCAGCCCGTGACCTCTGTCCAGC CTCCAGAAGAGCCTGAGCTGTCTGATCAGCCTGTTACCTCTGTGCAACCACCCGAGGAGCCTGAGCTGTC AGACCAGCCTGTGACAAGCGTCCAGCCCCCCGAGGAACCCGAACTCTCCGACCAGCCCGTAACAGGGTAC ACGTCTACATCCGAAGTGTCTCAAAAGCCCGATACTAAGAAGGACATAGACCTTGGTCCTGTGATGGAAG GTGGACCAGTCGGCGCGGGTGACGTGCAGAAGCAGCTGGAGACGATTGCCGAAGAGCCTGCAGCAGTTCC ACCCCTCGAGAGCGCATTCGGGTCACTGTATGCTTTTATCCTCTACCTGAGCAAGATGCTGCTCGCAACA TTGCCCGACAACGTTCAGCCGGGCCCTGATTTTTATGGGCTCCCGTGGCAGCCAGTGATTATTACAGCAG TGCTTGGCATTGTGTCTTTTGCAATATTCAGCTGGCGCACTATCCTGGTAGTGAAATCAAGAGTGTACCA GGTGACTGAGAAGCAAATCTCAGAAAAGCTTGAGAATATCAAGAAGGAGAATGCAGAGCTGATGCAGAAG CTTTCTTCTTACGAGCAGAAAATCAAAGAGTCAAAGAAGTATGTCCAGGAGACTAAGAAACAGAACATGA TTCTGAGTGACGAGGCCGTCAAGTACAAGGATAAGATCAAGATACTTGAAGAAACCAACGTGTCTCTGGG TGACAAGGCGAAGTCTCTGAGGCTTCAGCTGGAATCCGAGCGGGAACAGAATGTCAAGAATCAGGATCTC ATCCTCGAGAATAAGAAAAGTATCGAGAAGTTGAAGGATGTCATCTCCATGAACGCTAGCGAACTTAGCG AAGTGCAGGTCGCCCTGAACGAAGCCAAACTTAGTGAGGAGAACGTTAAGTCCGAATGCCACCGGGTCCA AGAGGAAAATGCGCGGCTGAAGAAAAAAAAAGAGCAGCTGCAGCAGCAGGTGGAGGAGTGGAGTAAGAGC CATGCCGAGCTCACGGGACAGATTAAGTCTTTCGAAAAAAGCCAGGAAGATTTGGAGATTGCTCTCACAC ATAAGGACGACAACATTAGCGCCTTGACCAATTGTATAACTCAGCTGAACCGCCTTGAGTGCGAGTTGGA GTCTGAGGACCCTGACAAGGGAGGTAATGAATCAGACGACCTCGCCAATGGTGAAACCGGCGGGGATAGA TCCGAAAAGATAAGGAACCGGATTAAGCAGATGATGGACGTTAGCAGGACCCAGACAGCCGTCTCCATCG TTGAAGAAGACCTCAAGCTGCTGCAGCTGAAGCTTAGGGCCAGCATGAGCACAAAATGTAACCTCGAGGA CCAGATTAAAAAGCTCGAAGACGACAGGTCATCTCTGCAGACAGCTAAAGCAGGTCTGGAAGACGAGTGC AAAACGCTTCGGCAGAAGGTAGAAATTCTGAATGAACTCTACCAGCAGAAAGAGATGGCGCTGCAGAAAA AGCTCTCTCAAGAAGAGTATGAAAGGCAAGATAGGGAGCAAAGGCTTACAGCCGCAGATGAGAAAGTGGT GCTGGCTGCCGAGGAAGTGAAGACCTACAAAAGGCGGATCGAGGAAATGGAGGAAGAACTCCAAAAGACA GAGCGAAGTTTTAAAAACCAGATCGCAGCTCACGAGAAGAAGGCCCACGACAATTGGCTCAAGGCAAGGG CTGCTGAGAGGGCAATGGCCGAGGAGAAGCGGGAAGCCGCAAATCTGCGACATAAACTGCTCGAAATGAC GCAGAAGATGGCTATGAGACAGGACGAGCCAGTTATCGTCAAGCCCATGCCTGGGCGACCAAACACACAA AACCCACCAAGGCGGGGCCTGCTCAGTCAGAACGGGTCTTTTGGCCCAAGCCCTGTGAGCGGCGGAGAAT GCTCACCGCCTCTTCCTGCAGAACCTCCTGGCAGACCACTTTCTGCGACCCTGTCCCGGAGAGACACCCC CAGAAGCGAGTTCGGGAGCCTCGATAGGCATCTGCCAAGACCTAGATGGCCTTCCGAAGCAAGTGGCAAG CATTCTGCCTCAGATCCCGGACCGGCCCCTGTGGTGAACTCATCATCCAGATCCTCCAGCCCTGCCAAAG CCGTCGATGAAGGCAAGGTAAACATGGCTCCCAAGGGCCCTCCACCATTTCCCGGAGTGCCTCTCATGGG GGGACCAGTGCCTCCCCCTATTCGCTACGGCCCACCCCCACAACTGTGTGGAGGGCCTTTTGGCCCTCGG CCTCTCCCCCCACCTTTTGTCCCGGGCATGCACCCCCCCCTGGGAGTTCGGGAATATGCTCCCGGGGTGC TTCCTGGCAAACGCGACCTGCCCCTGGACCCTAGAGAGTTCCTGCCAGGCCATACCCCGTTCCGGCCTCC AGGAAGCTTGGGTCCACGAGAGTTCTTCATACCCGGTACCAGGCTGCCTCCGCCGACACATGGCCCACAG GAATATCCTCCTCCGCCTCCAGCAGTCAGGGATAGTCTGCCATCCGGTCCTCGGGAGGAGGCCAAACCTG CTAGCCCATCTAGCGTGCAGGATAGGTCCCAGGCCTCTAAACCAACTCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR223502 representing NM_177389 Red=Cloning site Green=Tags(s) MAAAPGLLFWLFVLGALWWVPGQSDLSHGRRFSDLKVCGDEECSMLMYRGKALEDFTGPDCRFVNFKKGD DVYVYYKLAGGSLELWAGSVEHSFGYFPKDLIKVLHKYTEEELHIPADETDFVCFEGGRDDFNSYNVEEL LGSLELEDSVPEESKKAEEVSQHREKSPEESRGRELDPVPEPEAFRADSEDGEGAFSESTEGLQGQPSAQ ESHPHTSGPAANAQGVQSSLDTFEEILHDKLKVPGSESRTGNSSPASVEREKTDAYKVLKTEMSLDLKTK FGSTADALVSDDEATRLVTSLEDGFDEALDAEYYPMEEEEEVEEDADSSDELPLLTFSDKDEKVPGKPMI EKYLTDKDPNLSEEDKVEPPTWGDAFFSIVTGGEGKPGVVDLERSIEEEEDVSVSSSHQRKPQPAAGYTD SEDEGDDLFVEEPKTNDVKDSETDPELVITGEEKDIQESRKGLVQPESQSEDAKSETASAYRLQGSKLNP LSAAEKGRDFTLKAVFEKKENGLKESVIHISKETLHEDKTREIQRDSLESELVHRALGSSVTENNKPKSL GVAPLLGNNKPDASKDSTEVPDGSVSGPKAGQQEGFLEPGLKTQHQPRFSPPEETGPSRELGGKVPISGR NLSWQQEQDVAAVVGKHANEKTGFPEEESREDGTDAEQARAIRRPQEAESPEVLSVQPGRPDEEEEEEEG DNYPPEGLMEDENAVSAQQSRENSPSARDGRSDMNSQVFEKVILGTLNLNTEKTKQPANMILETGQESET TSEEAGDVGKESGHSVVVDSEESHLADMRAQRPSQVHGLRDETAAQTPGSGEAVLSKNPNDLQKDNPEEE LVNTLGLEDPGVGEISEGEPEDTKEFGVSESQGTDAEDLRDDPSRQATPEIPDIVLKSIREDLPIINSFF KDDQQSLHRFLKYFDVRELEGLLEDMSIRLRSAHQNSLPYNMEKVLDKVFRASESRILSMAEKMLDTGVA KNRDLGSKESSPLEEAEVLDDIQDLIYFVRYQYSGVETAPLVTPPPPEEGWARPGEERQPPQQDSLPQEN TGDLSVQPPEEPELSDQPVTSVQPPEEPELSDQPVTSVQPPEEPELSDQPVTSVQPPEEPELSDQPVTGY TSTSEVSQKPDTKKDIDLGPVMEGGPVGAGDVQKQLETIAEEPAAVPPLESAFGSLYAFILYLSKMLLAT LPDNVQPGPDFYGLPWQPVIITAVLGIVSFAIFSWRTILVVKSRVYQVTEKQISEKLENIKKENAELMQK LSSYEQKIKESKKYVQETKKQNMILSDEAVKYKDKIKILEETNVSLGDKAKSLRLQLESEREQNVKNQDL ILENKKSIEKLKDVISMNASELSEVQVALNEAKLSEENVKSECHRVQEENARLKKKKEQLQQQVEEWSKS HAELTGQIKSFEKSQEDLEIALTHKDDNISALTNCITQLNRLECELESEDPDKGGNESDDLANGETGGDR SEKIRNRIKQMMDVSRTQTAVSIVEEDLKLLQLKLRASMSTKCNLEDQIKKLEDDRSSLQTAKAGLEDEC KTLRQKVEILNELYQQKEMALQKKLSQEEYERQDREQRLTAADEKVVLAAEEVKTYKRRIEEMEEELQKT ERSFKNQIAAHEKKAHDNWLKARAAERAMAEEKREAANLRHKLLEMTQKMAMRQDEPVIVKPMPGRPNTQ NPPRRGLLSQNGSFGPSPVSGGECSPPLPAEPPGRPLSATLSRRDTPRSEFGSLDRHLPRPRWPSEASGK HSASDPGPAPVVNSSSRSSSPAKAVDEGKVNMAPKGPPPFPGVPLMGGPVPPPIRYGPPPQLCGGPFGPR PLPPPFVPGMHPPLGVREYAPGVLPGKRDLPLDPREFLPGHTPFRPPGSLGPREFFIPGTRLPPPTHGPQ EYPPPPPAVRDSLPSGPREEAKPASPSSVQDRSQASKPTP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_177389
ORF Size	5790 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_177389.3, NP_796363.2
RefSeq Size	6933 bp
RefSeq ORF	5793 bp
Locus ID	338366
UniProt ID	Q8BI84
MW	213.7 kDa
Gene Summary	Plays a role in the transport of cargos that are too large to fit into COPII-coated vesicles and require specific mechanisms to be incorporated into membrane-bound carriers and exported from the endoplasmic reticulum. This protein is required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. It may participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. It is also specifically required for the secretion of lipoproteins by participating in their export from the endoplasmic reticulum. Required for correct assembly of COPII coat components at endoplasmic reticulum exit sites (ERES) and for the localization of SEC16A and membrane-bound ER-resident complexes consisting of MIA2 and PREB/SEC12 to ERES.[UniProtKB/Swiss-Prot Function]

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