Acacb (NM_133904) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Acc2; Accb; AI597064; AW743042
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR223495 representing NM_133904 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCTTGCTTCTCTTTCTGACTTGCCTGGTTTTCTCCTGCCTGACCTTTTCCTGGCTAAAAATCTGGG GGAAGATGACAGACTCGAAGCCGCTCACCAACAGTAAGGTGGAAGCCAATCTCCTTTCAAGCGAGGAGTC CCTTTCAGCCTCAGAGCTGTCAGGGGAACAGCTGCAGGAACATGGCGACCACAGCTGTCTGTCCTACAGA GGTCCCAGAGACGCCAGTCAGCAGAGGAACTCCCTGCCAAGCTCATGCCAGAGGCCTCCGAGGAACCCAC TGTCTTCCAATGACACCTGGCCCTCCCCAGAACTCCAAACCAACTGGACAGCCGCCCCGGGCCCAGAGGT TCCAGATGCTAATGGGTTGTCCTTCCCAGCCAGGCCCCCGAGCCAGAGGACAGTATCCCCCTCCAGAGAG GACAGGAAGCAGGCACACATCAAGAGACAGCTGATGACCAGCTTTATCCTGGGCTCCCTGGATGACAACT CCTCTGATGAGGACCCTAGTGCCGGCTCCTTCCAGAACTCCTCCCGGAAGAGCAGCAGGGCTAGCCTGGG CACTCTGTCCCAGGAGGCTGCATTGAACACAAGTGATCCTGAATCTCACGCGCCTACTATGAGGCCCAGC ATGTCTGGACTCCATCTGGTGAAGAGAGGCCGAGAACACAAGAAACTGGACCTGCACAGAGATTTCACCG TCGCGTCCCCAGCCGAGTTTGTCACTCGGTTTGGAGGCAACAGGGTCATAGAGAAGGTGCTCATCGCCAA CAATGGCATCGCTGCGGTCAAGTGTATGCGCTCCATCCGCCGCTGGGCCTACGAGATGTTCCGTAATGAA CGTGCCATCCGGTTCGTGGTCATGGTGACGCCCGAGGATCTGAAGGCCAACGCAGAGTACATCAAGATGG CCGATCAGTACGTCCCTGTCCCAGGAGGACCCAATAACAACAACTATGCCAATGTTGAGCTGATCATAGA CATTGCCAAGAGAATCCCTGTACAGGCCGTGTGGGCTGGCTGGGGCCACGCTTCGGAAAACCCCAAACTT CCAGAGCTGCTGTGTAAACACGAGATTGCTTTCCTAGGTCCCCCCAGTGAGGCCATGTGGGCCCTGGGAG ACAAGATCGCCTCCACCATCGTAGCGCAGACACTGCAAATCCCAACCTTACCCTGGAGTGGAAGCGGTCT CACAGTGGAGTGGACGGAGGACAGCCGGCACCAGGGCAAATGCATCAGTGTCCCCGAAGACGTTTACGAG CAGGGCTGTGTGAAGGATGTGGACGAAGGCTTGCAGGCAGCAGAAAAAATAGGATTTCCTTTGATGATCA AAGCCTCCGAAGGCGGAGGCGGGAAAGGTATCCGCAAGGCTGAGAGTGCAGAGGACTTCCCGATGCTTTT CCGACAAGTGCAGAGTGAGATTCCGGGCTCGCCGATCTTTCTAATGAAGCTGGCCCAGAACGCACGGCAC CTGGAGGTCCAGGTCTTGGCAGATCAGTATGGAAACGCGGTGTCACTGTTCGGACGGGACTGTTCCATTC AGAGGCGGCACCAGAAGATCATTGAGGAGGCTCCGGCCACCATCGCCGCGCCTGCCGTGTTTGAGTTCAT GGAACAGTGTGCTGTCCTCCTGGCCAAGATGGTGGGTTATGTGAGCGCGGGGACTGTGGAGTACTTATAC AGCCAGGATGGCAGCTTCCACTTCCTGGAGCTGAACCCACGCCTGCAGGTGGAGCATCCGTGCACTGAAA TGATCGCAGATGTCAACCTGCCTGCTGCGCAGTTGCAGATCGCCATGGGCGTGCCCCTGCACCGACTGAA GGACATACGGCTTCTGTACGGAGAGTCCCCCTGGGGAGTGACCCCCATTCCTTTTGAAACCCCCTTGAGC CCTCCCATTGCCCGAGGCCACGTCATTGCAGCCAGGATCACCAGCGAAAACCCAGATGAGGGCTTTAAGC CAAGCTCGGGGACAGTGCAGGAGCTGAACTTCCGCAGCAACAAGAACGTGTGGGGTTACTTCAGCGTGGC CGCTGCTGGGGGCTTGCACGAGTTCGCCGATTCCCAGTTTGGGCACTGCTTCTCCTGGGGCGAGAACCGT GAAGAGGCCATTTCGAACATGGTAGTGGCTTTGAAGGAACTGTCCATCCGGGGTGACTTCCGGACCACTG TGGAGTATCTCGTTAACCTTCTGGAGACCGAGAGCTTCCAGAACAACGATATCGACACGGGGTGGCTGGA CCACCTCATCGCTCAGCGGGTGCAGGCAGAGAAGCCGGACATCATGCTTGGGGTGGTGTGTGGGGCCTTG AATGTGGCAGACGCGATGTTCAGAACCTGCATGACGGAATTCTTGCACTCCCTGGAAAGGGGTCAGGTCC TCCCTGCTGATTCTCTGCTGAACATTGTGGACGTTGAATTGATTTACGGCGGCATCAAGTATGCTCTCAA GGTAGCCCGACAGTCCCTGACTATGTTTGTCCTCATCATGAACGGCTGCCACATCGAGATCGATGCCCAC CGGCTGAACGATGGGGGGCTGCTCCTGTCCTACAATGGCAGCAGTTACACTACGTACATGAAGGAAGAGG TTGACAGTTACCGGATCACTATCGGCAATAAGACTTGCGTGTTTGAAAAGGAAAACGACCCCACTGTCCT GAGATCCCCCTCGGCTGGGAAGCTGATGCAGTATACGGTGGAGGACGGAGACCACGTGGAAGCTGGGAGC AGCTACGCGGAGATGGAGGTGATGAAGATGATCATGACCCTGAACGTGCAGGAGAGCGGCCGGGTGAAGT ACATCAAGCGGCCAGGGGTTATACTGGAGGCTGGCTGCGTGGTGGCGAGGCTAGAACTCGATGACCCTTC AAAAGTGCACGCGGCCCAGCCGTTTACTGGGGAGCTCCCTGCCCAGCAGACTCTGCCCATCCTCGGGGAG AAGCTACACCAAGTGTTCCACGGCGTCTTGGAAAATCTGACCAATGTCATGAGTGGCTACTGCCTGCCCG AGCCCTTCTTCAGCATGAAGCTGAAGGACTGGGTCCAGAAGCTCATGATGACGCTCCGGCATCCCTCCCT ACCTCTGCTGGAGCTACAGGAGATCATGACCAGTGTGGCCGGCCGCATCCCCGCCCCGGTGGAGAAGGCA GTCCGCAGGGTGATGGCCCAGTACGCCAGTAACATCACCTCCGTGCTATGCCAGTTCCCCAGCCAGCAGA TAGCCACCATCCTGGACTGCCATGCCGCCACCCTGCAGCGTAAGGCGG[AT]CCGAGAGGTCTTCTTCAT GAACACACAGAGCATCGTGCAGCTGGTCCAGAGATACCGCAGCGGGACCCGTGGCTACATGAAGGCTGTG GTGCTGGACCTCCTGAGGAAATATCTAAACGTGGAGCACCATTTTCAGCAAGCCCACTATGACAAGTGTG TGATCAACCTGAGGGAGCAGTTCAAGCCGGACATGACCCAGGTGCTGGACTGCATCTTCTCACACTCCCA GGTGGCCAAGAAGAACCAGCTGGTGACCATGCTGATAGATGAGCTGTGCGGCCCAGACCCCACCCTGTCA GACGAGCTGACCTCCATCCTCTGTGAGCTCACGCAGCTGAGCAGAAGCGAGCACTGCAAGGTTGCCCTCA GAGCCAGACAGGTCCTGATCGCCTCCCACCTCCCCTCCTACGAGCTGCGGCACAACCAGGTGGAGTCCAT CTTCCTGTCCGCCATCGACATGTACGGCCACCAGTTCTGCCCAGAAAATCTCAAGAAACTAATACTTTCA GAAACGACCATATTCGACGTCCTGCCCACTTTCTTCTATCACGAGAACAAGGTTGTCTGTATGGCGTCCC TGGAGGTTTACGTGCGGAGAGGCTACATCGCCTACGAGCTAAACAGCCTGCAGCACCGGGAGCTCCCAGA TGGCACCTGTGTGGTGGAGTTCCAGTTCATGCTGCCCTCTTCCCACCCCAACCGGATGGCCGTGCCCATC AGTGTCTCCAACCCCGACCTGCTGAGGCACAGCACAGAACTCTTCATGGACAGTGGCTTCTCCCCACTGT GCCAGCGGATGGGGGCCATGGTGGCCTTCAGGAGATTTGAAGAATTCACCAGGAACTTTGATGAAGTCAT CTCCTGCTTTGCCAACGTGCAGACAGACACTCTTCTCTTCAGTAAGGCGTGCACTTCCCTGTACTCAGAG GAAGACAGCAAGAGCCTCCGAGAGGAACCCATCCACATCCTGAATGTGGCCATCCAGTGTGCCGACCACA TGGAGGACGAGGCGCTGGTGCCAGTCTTCCGTGCCTTTGTACAGTCCAAGAAGCACATCCTTGTGGATTA CGGACTCCGAAGAATCACATTCCTTGTTGCCCAAGAGAGAGAATTCCCCAAGTTCTTCACGTTCAGAGCG AGGGATGAGTTTGCAGAAGACCGGATTTATCGCCACTTGGAGCCTGCCCTGGCCTTCCAGCTGGAGCTGA GCCGGATGCGCAACTTTGACCTGACCGCTGTGCCCTGTGCCAACCACAAGATGCATCTTTACCTGGGAGC CGCTAAGGTGAAGGAAGGGTTGGAGGTGACCGACCACAGGTTCTTTATCCGAGCCATTATCCGGCACTCA GACCTGATCACCAAGGAAGCCTCCTTCGAGTACCTGCAGAACGAAGGTGAGCGGCTGCTGCTGGAAGCCA TGGACGAGCTGGAGGTGGCGTTCAACAACACCAGTGTGCGCACTGACTGCAACCACATCTTCCTCAACTT TGTGCCCACAGTCATCATGGACCCGCTCAAGATCGAGGAGTCGGTGCGTGACATGGTCATGCGCTATGGC AGCCGTCTGTGGAAGCTCCGAGTGCTGCAGGCCGAGGTTAAGATCAACATCCGTCAGACAACCTCAGATA GTGCCATCCCCATCCGCCTCTTCATCACCAACGAGTCTGGCTACTACCTGGACATCAGCCTCTATAGAGA AGTGACGGACTCCAGATCCGGAAATATCATGTTTCATTCCTTTGGCAACAAGCAAGGTAGCCTGCATGGG ATGCTGATCAATACACCCTATGTCACCAAGGACCTGCTCCAGGCTAAGCGATTCCAGGCACAGTCCCTCG GGACCACCTATGTGTACGACTTCCCAGAGATGTTCAGGCAGGCTCTCTTTAAACTGTGGGGCTCCCCAGA GAAGTACCCCAAAGATATCCTGACATACACAGAGCTGGTGTTGGACTCTCAAGGTCAGCTGGTGGAGATG AACCGGCTTCCTGGTTGTAACGAGGTGGGCATGGTGGCCTTCAAAATGAGGTTCAAGACCCCGGAGTATC CAGAAGGCCGGGACGCCGTTGTCATCGGCAATGACATCACCTTCCAAATCGGCTCTTTCGGCATAGGGGA GGACTTCCTGTACCTGCGAGCGTCTGAGATGGCCCGGACAGAGGGCATCCCCCAAATCTACCTGGCAGCT AACAGCGGGGCCCGTATGGGCCTGGCTGAGGAGATCAAACAGATATTCCAAGTGGCTTGGGTGGACCCAG AGGATCCCCACAAAGGATTTAGATACCTGTACCTGACGCCCCAAGACTACACCCAGATCAGTTCCCAGAA CTCAGTGCACTGCAAGCACATCGAAGATGAAGGCGAATCCAGATACGTCATCGTGGATGTCATCGGGAAG GATGCCAACCTGGGTGTGGAGAACCTGAGGGGCTCGGGCATGATTGCAGGAGAAGCTTCCCTGGCTTATG AGAAAACGGTCACCATCAGCATGGTGACCTGCCGCGCCCTTGGAATCGGGGCTTACCTGGTGAGGCTTGG CCAGCGGGTGATCCAGGTGGAAAACTCCCACATCATCCTCACCGGAGCCGGTGCTCTCAACAAGGTCCTG GGAAGAGAGGTCTACACGTCCAACAACCAGCTAGGTGGCGTGCAGATCATGCATACCAACGGGGTCTCCC ACGTCACCGTGCCAGATGACTTCGAGGGGGTCTGCACCATTCTGGAATGGCTGTCATTTATACCGAAGGA CAATCGCAGCCCGGTCCCCATCACCACTCCTTCTGACCCCATCGACAGGGAAATTGAATTCACCCCAACC AAAGCTCCCTACGACCCCAGGTGGATGCTTGCAGGCAGGCCTCACCCAACTCTGAAGGGGACCTGGCAGA GTGGATTCTTCGACCATGGCAGTTTCAAGGAAATCATGGCACCCTGGGCCCAGACTGTGGTGACGGGACG AGCAAGGCTGGGGGGCATCCCTGTGGGAGTGATTGCTGTGGAGACTCGGACTGTGGAGGTGGCTGTCCCC GCTGACCCTGCCAACTTGGATTCTGAGGCCAAGATCATCCAGCAGGCGGGCCAGGTGTGGTTCCCGGACT CGGCCTACAAGACGGCGCAGGTCATCAGGGACTTCAACAAGGAGCGCCTGCCTCTCATGATCTTCGCCAA CTGGAGGGGCTTCTCCGGAGGCATGAAGGACATGTATGAGCAGATGCTGAAGTTTGGCGCCTACATCGTG GATGGTCTCCGCCTGTACGAGCAGCCCATTCTCATCTACATCCCTCCCTGTGCGGAGCTCCGTGGGGGCT CCTGGGTTGTCCTGGACTCCACCATCAACCCCCTGTGCATAGAGATGTATGCAGACAAAGAGAGCAGGGG GGGTGTTCTGGAGCCGGAGGGCACGGTGGAGATTAAGTTCCGGAAGAAAGATCTGGTGAAAACCATAAGG AGGATAGACCCAGTGTGTAAGAAGCTCGTGGGACAGCTGGGGAAAGCCCAGCTTCCCGACAAGGACCGGA AGGAGCTGGAGGGCCAGCTGAAGGCCCGGGAGGAACTGCTGCTTCCCATCTACCACCAGGTGGCAGTGCA GTTCGCAGACCTGCACGACACCCCGGGCCACATGCTGGAGAAAGGAATCATTTCTGATGTGCTGGAATGG AAGACTGCCCGCACCTTCTTCTACTGGAGGCTGCGCCGGCTGCTGCTGGAGGCCCAGGTGAAGCAGGAGA TACTCCGAGCCAGCCCCGAGCTGAACCACGAGCACACACAGTCCATGCTGCGACGCTGGTTCGTGGAGAC TGAGGGCGCTGTCAAGGCCTACCTGTGGGACAGCAACCAGGTGGTGGTCCAGTGGCTGGAACAGCACTGG TCAGCCAAGGACGGCTTGCGCTCCACCATCCGTGAAAACATCAATTATCTGAAGCGGGACTCTGTCCTCA AGACCATCCAAAGTCTGGTCCAAGAACATCCAGAGGTCATCATGGACTGCGTGGCCTACCTGAGCCAGCA CCTCACGCCAGCCGAGCGGATACAGGTGGCTCAGCTGCTGTCTACCACGGAGAGCCCAGCTTCCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR223495 representing NM_133904 Red=Cloning site Green=Tags(s) MVLLLFLTCLVFSCLTFSWLKIWGKMTDSKPLTNSKVEANLLSSEESLSASELSGEQLQEHGDHSCLSYR GPRDASQQRNSLPSSCQRPPRNPLSSNDTWPSPELQTNWTAAPGPEVPDANGLSFPARPPSQRTVSPSRE DRKQAHIKRQLMTSFILGSLDDNSSDEDPSAGSFQNSSRKSSRASLGTLSQEAALNTSDPESHAPTMRPS MSGLHLVKRGREHKKLDLHRDFTVASPAEFVTRFGGNRVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNE RAIRFVVMVTPEDLKANAEYIKMADQYVPVPGGPNNNNYANVELIIDIAKRIPVQAVWAGWGHASENPKL PELLCKHEIAFLGPPSEAMWALGDKIASTIVAQTLQIPTLPWSGSGLTVEWTEDSRHQGKCISVPEDVYE QGCVKDVDEGLQAAEKIGFPLMIKASEGGGGKGIRKAESAEDFPMLFRQVQSEIPGSPIFLMKLAQNARH LEVQVLADQYGNAVSLFGRDCSIQRRHQKIIEEAPATIAAPAVFEFMEQCAVLLAKMVGYVSAGTVEYLY SQDGSFHFLELNPRLQVEHPCTEMIADVNLPAAQLQIAMGVPLHRLKDIRLLYGESPWGVTPIPFETPLS PPIARGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENR EEAISNMVVALKELSIRGDFRTTVEYLVNLLETESFQNNDIDTGWLDHLIAQRVQAEKPDIMLGVVCGAL NVADAMFRTCMTEFLHSLERGQVLPADSLLNIVDVELIYGGIKYALKVARQSLTMFVLIMNGCHIEIDAH RLNDGGLLLSYNGSSYTTYMKEEVDSYRITIGNKTCVFEKENDPTVLRSPSAGKLMQYTVEDGDHVEAGS SYAEMEVMKMIMTLNVQESGRVKYIKRPGVILEAGCVVARLELDDPSKVHAAQPFTGELPAQQTLPILGE KLHQVFHGVLENLTNVMSGYCLPEPFFSMKLKDWVQKLMMTLRHPSLPLLELQEIMTSVAGRIPAPVEKA VRRVMAQYASNITSVLCQFPSQQIATILDCHAATLQRKAXXREVFFMNTQSIVQLVQRYRSGTRGYMKAV VLDLLRKYLNVEHHFQQAHYDKCVINLREQFKPDMTQVLDCIFSHSQVAKKNQLVTMLIDELCGPDPTLS DELTSILCELTQLSRSEHCKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCPENLKKLILS ETTIFDVLPTFFYHENKVVCMASLEVYVRRGYIAYELNSLQHRELPDGTCVVEFQFMLPSSHPNRMAVPI SVSNPDLLRHSTELFMDSGFSPLCQRMGAMVAFRRFEEFTRNFDEVISCFANVQTDTLLFSKACTSLYSE EDSKSLREEPIHILNVAIQCADHMEDEALVPVFRAFVQSKKHILVDYGLRRITFLVAQEREFPKFFTFRA RDEFAEDRIYRHLEPALAFQLELSRMRNFDLTAVPCANHKMHLYLGAAKVKEGLEVTDHRFFIRAIIRHS DLITKEASFEYLQNEGERLLLEAMDELEVAFNNTSVRTDCNHIFLNFVPTVIMDPLKIEESVRDMVMRYG SRLWKLRVLQAEVKINIRQTTSDSAIPIRLFITNESGYYLDISLYREVTDSRSGNIMFHSFGNKQGSLHG MLINTPYVTKDLLQAKRFQAQSLGTTYVYDFPEMFRQALFKLWGSPEKYPKDILTYTELVLDSQGQLVEM NRLPGCNEVGMVAFKMRFKTPEYPEGRDAVVIGNDITFQIGSFGIGEDFLYLRASEMARTEGIPQIYLAA NSGARMGLAEEIKQIFQVAWVDPEDPHKGFRYLYLTPQDYTQISSQNSVHCKHIEDEGESRYVIVDVIGK DANLGVENLRGSGMIAGEASLAYEKTVTISMVTCRALGIGAYLVRLGQRVIQVENSHIILTGAGALNKVL GREVYTSNNQLGGVQIMHTNGVSHVTVPDDFEGVCTILEWLSFIPKDNRSPVPITTPSDPIDREIEFTPT KAPYDPRWMLAGRPHPTLKGTWQSGFFDHGSFKEIMAPWAQTVVTGRARLGGIPVGVIAVETRTVEVAVP ADPANLDSEAKIIQQAGQVWFPDSAYKTAQVIRDFNKERLPLMIFANWRGFSGGMKDMYEQMLKFGAYIV DGLRLYEQPILIYIPPCAELRGGSWVVLDSTINPLCIEMYADKESRGGVLEPEGTVEIKFRKKDLVKTIR RIDPVCKKLVGQLGKAQLPDKDRKELEGQLKAREELLLPIYHQVAVQFADLHDTPGHMLEKGIISDVLEW KTARTFFYWRLRRLLLEAQVKQEILRASPELNHEHTQSMLRRWFVETEGAVKAYLWDSNQVVVQWLEQHW SAKDGLRSTIRENINYLKRDSVLKTIQSLVQEHPEVIMDCVAYLSQHLTPAERIQVAQLLSTTESPASS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_133904
ORF Size	7347 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_133904.1, NM_133904.2, NP_598665.2
RefSeq Size	8497 bp
RefSeq ORF	7347 bp
Locus ID	100705
UniProt ID	E9Q4Z2
MW	276.2 kDa
Gene Summary	Catalyzes the ATP-dependent carboxylation of acetyl-CoA to malonyl-CoA (By similarity). Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase (By similarity). Involved in inhibition of fatty acid and glucose oxidation and enhancement of fat storage (PubMed:11283375, PubMed:12920182, PubMed:15677334, PubMed:18487439, PubMed:22362781). May play a role in regulation of mitochondrial fatty acid oxidation through malonyl-CoA-dependent inhibition of carnitine palmitoyltransferase 1 (PubMed:10677481).[UniProtKB/Swiss-Prot Function]

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