Hs3st3b1 (NM_018805) Mouse Tagged ORF Clone

CAT#: MR223170

  • TrueORF®

Hs3st3b1 (Myc-DDK-tagged) - Mouse heparan sulfate (glucosamine) 3-O-sulfotransferase 3B1 (Hs3st3b1)

ORF Plasmid: DDK tGFP



  "NM_018805" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 3-OST-3B; 3Ost3b; 3OST3B1; AU041882; AW536289; Hs3st3b; m3-OST-3B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR223170 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCAGCGCCTGAGCGGCGGCCGGTCCTGTCTCGATGTCCCGGGCCGGTTCCTGCCGCAGCCGCCGC
CGCCCCCGCCGCCGGTGAGGAGGAAGCTCGCGCTGCTCTTCGCCATGCTTTGCATCTGGCTGTACATGTT
TTTGTACTCGTGCGCCGGCTCCTGTACCGCCGCTCCCGGCCTGCTGCTCCTGGGCTCGGGGTCCCGCGCT
ACTCACGCTCAGCCAGCCCTGGTCACAGCTCCGAATGAGACATCCCCTAAGATGCCCTTCCGGGCACCTC
CGGCCAATTCGCTGGCTGCAGGCAAGGATAAGACAGTAGGCGCTGGGAGCCAGGAGGAGCAGAGTCCAGA
GGCGCCGGACTCCCCCAGTCCCATCTCCAGCTTCTTCAGCGGCGCTGGGAGCAAGCAGCTGCCGCAGGCC
ATCATCATCGGGGTGAAGAAGGGCGGCACGCGGGCGTTGCTGGAGTTCCTGCGCGTGCACCCCGACGTGC
GCGCCGTGGGCGCCGAGCCCCACTTTTTCGACCGCAGCTACCACAAGGGTCTTGCCTGGTACCGGGACTT
GATGCCCAGAACCCTCGAGGGACAGATCACCATGGAGAAGACGCCCAGCTACTTTGTGACCCGGGAGGCA
CCCGCGCGCATCTCGGCCATGTCCAAGGACACCAAGCTCATCGTGGTGGTGCGCGACCCGGTGACCAGGG
CCATCTCGGACTACACGCAGACGCTGTCCAAGCGGCCGGACATCCCCAGCTTCGAGAGCCTGACGTTCCG
CAACCGCAGCGCGGGCCTCATAGACACGTCCTGGAGCGCCATCCAGATCGGCCTGTACGCCAAGCACCTG
GAGCCCTGGCTGCGCCACTTCCCGCTCGGCCAGATGCTCTTCGTGAGCGGGGAGCGGCTGGTCAGCGACC
CGGCCGGCGAGCTGCGCCGCGTGCAGGATTTCCTGGGCCTCAAGCGCATCATCACCGACAAGCACTTCTA
CTTCAACCAGACCAAGGGCTTCCCGTGCCTCAAGAAGGCGGAGGGCAGCGGCAAGCCGCACTGCCTGGGC
AAGACTAAGGGCCGCGCGCACCCCACCATCGCGCGCGAGGTGCTGCGGCAGCTGCGTGACTTTTACCGGC
CCTTCAACCGCAAGTTCTACCAGATGACCGGCCGCGACTTCGGCTGGGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR223170 protein sequence
Red=Cloning site Green=Tags(s)

MGQRLSGGRSCLDVPGRFLPQPPPPPPPVRRKLALLFAMLCIWLYMFLYSCAGSCTAAPGLLLLGSGSRA
THAQPALVTAPNETSPKMPFRAPPANSLAAGKDKTVGAGSQEEQSPEAPDSPSPISSFFSGAGSKQLPQA
IIIGVKKGGTRALLEFLRVHPDVRAVGAEPHFFDRSYHKGLAWYRDLMPRTLEGQITMEKTPSYFVTREA
PARISAMSKDTKLIVVVRDPVTRAISDYTQTLSKRPDIPSFESLTFRNRSAGLIDTSWSAIQIGLYAKHL
EPWLRHFPLGQMLFVSGERLVSDPAGELRRVQDFLGLKRIITDKHFYFNQTKGFPCLKKAEGSGKPHCLG
KTKGRAHPTIAREVLRQLRDFYRPFNRKFYQMTGRDFGWD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_018805
ORF Size 1173 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_018805.1, NM_018805.2, NP_061275.2
RefSeq Size 6004 bp
RefSeq ORF 1173 bp
Locus ID 54710
UniProt ID Q9QZS6
MW 43.3 kDa
Gene Summary Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to an N-unsubstituted glucosamine linked to a 2-O-sulfo iduronic acid unit on heparan sulfate. Catalyzes the O-sulfation of glucosamine in IdoUA2S-GlcNS and also in IdoUA2S-GlcNH2. Unlike 3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...