Ahctf1 (NM_026375) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	6230412P20Rik; AV011447; Elys
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR223137 representing NM_026375 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAGACTTGACAGCTCAAGTGACTAGTGATCTCCTGCATTTCCCAGAAGTGACTATTGAAGCTCTTG GAGAAGATGAGATAACATTAGAGTCCGTGCTTCGTGGAAAGTTTGCTGCAGGGAAAAATGGACTAGCATG CTTAGCTTGTGGTCCACAACTTGAAGTTGTAAACTCCCTAACAGGAGAGCGGTTATCTGCATATAGATTC AGTGGAGTAAATGAACAGCCTCCTGTAGTCCTTGCAGTGAAAGAATTCTCTTGGCATAAGAGGACTGGAT TGTTAATAGGATTGGAAGAAGCAGATGGGAGTGTTCTTTGTCTTTATGACCTTGGTATATCAAGAGTGGT CAAAGCAGTTGTTCTTCCTGGAAGGGTAACAGCTATCGAGCCTATAATTAACCATGGAGGAGCCAGTGCG AGTACCCAGCATTTACATCCAAGTCTCCGGTGGCTTTTTGGCGTGGCCGCTGTGGTGACTGATGTTGGAC AGATCCTTCTTATTGACCTGTGTTTGGATGACTTGTCCTGCAGTCAGAATGAAGTTGAGGCATCAGACCT TGAAGTTATCACTGGTATCCCAGCTGAAGTACCACACATCAGAGAGAGAGTGATGAGAGAGGGGCGCCAC CTGTGCTTCCAGTTAGTAAGCCCATTGGGAGTAGCCATTTCTACTCTGAGTTACATCAACAGGACAAATC AGCTTGCTGTGGGTTTTTCTGATGGCTACTTAGCACTTTGGAACATGAAAAGCATGAAAAGAGAATACTA TACACAGTTGGAAGGTGGAAGGGTTCCTGTCCATGCAGTTGCCTTTCAAGAGCCTGAGAATGATCCTCGT AACTGCTGTTATTTATGGGCTGTTCAGTCCACACAAGATAGTGAAGGGGATGTTTTGAGTTTGCATCTGC TTCAGCTGGCTTTTGGTGATAGAAAATGTTTGGCATCAGGGCAAATTTTATATGAGGGATTAGAATACTG CGAAGAAAGATATACACTGGATCTAGCAGGTGGCACGTTCCCCTTAAGGGGACAAACTAGTAATACCAAA TTGTTGGGATGCCAGAGTATAGAGAGATTTCCATCTCATGGAGACAGAGAAGAAAGTATGAGAGAAGCTC TGTCTCCCGATACCAGCGTTTCTGTCTTTACCTGGCAAGTGAATATATATGGACAGGGAAAGCCTTCTGT GTATTTAGGGCTATTTGACATAAATCGTTGGTATCATGCACAAATGCCCGATTCTTTAAGATCAGGAGAA TCTCTGCATAATTGCTCTTATTTTGCGTTGTGGTCATTGGATTCGGTTGTAAGTAGGACTTCTCCACATC ACATCTTGGACATACTAGTACATGAGAGGAGTTTAAACCGAGGGGTTCCTCCTTCCTACCCACCTCCAGA GCAATTTTTTAACCCAAGTACTTTTAATTTTGATGCCACTTGTTTGTTAGACTCTGGAGTTATCCATGTA ACTTGTGCTGGATTTCAGAAGGAGACTTTGACATTTTTAAAGAAATCAGGACCAACTCTTAATGAAGTCA TTCCTGATAGTTATAATCGATGTCTTGTTGCTGGTCTCCTCTCACCAAGACTTATTGATATTCAGCCTTC CAGTTTAAGTCAAGAAGAACAATTAGAAGCTATATTGTCAGCAGCAATTCAGACAAGTTCCTTGGGACTT TTGACTGGTTACATCAGAACGTGGATAATAGAAGAACAACCAAATTCTGCTGCTAATCTACGATTTGTTC TTGAGTGGACATGGAATAAAGTGGTTCTCACAAAAGAAGAGTTTGATAGGCTTTGTGTGCCGCTGTTTGA CGGTTCGTGTCGTTTTATTGACCCACAGACTATTCAGTCTATCCAGCAGTGCCATTTACTGCTTAGCAAC CTTAGTACAGTCTTAAGCTGTTTTGCAATGGAGGCCCAGGGTATCACTGAGAGAGGACTGGTGGACTTGA GCAACAAGCACATGGTCACCCAGCTTCTCTGTCAGTACGCACACATGGTTCTGTGGTTCTGCCACTCGGG GCTTCTGCCCGAAGGCTTAGATGATGCTCTGCAGCTGTCAAGACTACGCTACAACTACCCTGTAATTCAG AACTACTATACAAGTCGTCGGCAGAAGTCTGAGCGCTCACCCAGAGGGAAGTGGAACCACGACTGCTTGA TGATTGATGGATTAGTCTCTCAACTAGGAGATGAAGTTGAGAAGTTGTGGAAGCGGGACGAAGGTGGCAC AGGAAGATACCCTCCTGCTAGCATCCACGCATTACTTGATATATATTTATTAGACAACATTACCGAAGCA AGCAAACATGCTATTACCATTTATTTGCTGCTTGATATTATGTATTCCTTTCCAAATAAAACGGATACCC CCATTGAATCTTTCCCAACTGCCTTTGCTATTTCTTGGGGCCAAGTTAAGCTAGTTCAAGGATTTTGGCT GCTAGATCATAATGACTATGAGAATGGTTTAGACCTTCTGTTTCACCCAGTTACTGCAAAGCCTGCATCG TGGCAACATTCAAAGATAATTGAAGCTTTTATGAGTCAGGGAGAGCACAAACAGGCTCTCCGGTATCTTC AGACAATGAAGCCAACAGTGTCCAGTAGCAATGAAGTTATCCTTCACCTCACTGTTCTACTTTTTAATAG ATGCATGGTTGAGGCCTGGAACTTACTGCGACAGAATTCAAACAGAGTAAATATAGAGGAATTATTAAAG CACGCTTATGAAGTTTGTCAGGAGATGGGCTTAATGGAGGATTTACTGAAGCTGCCATTTACAAACACTG AGCAGGAATGCTTAGTGAAATTTTTACAGTCCAGTACCAGTGTTGAGAATCATGAATTCCTTCTAGTTCA CCATTTACAGCGTGCCAATTATATTTCTGCCTTGAAACTAAACCAGATTCTGAAGAATAATCTCATGAGT GATCGTGACCCTCGATTGCGGGAAAGATCGGTGACTCGAAATTCTATATTAGACCAGTATGGGAAAATCC TACCTAGAGTCCAGAGAAAGTTAGCTGTTGAGCGAGCTAAGCCTTACCACCTGTCGACATCCTCAGTTTT TCATGAAGTTTCTAGACCCAAACCGTTATCGGCATTTCCAAAGAAAGCTATAACTGGAACAGTGTTAACC CGATCTACGTTCATCAGCAATGTTTTATCTAAAATTGGAGAGGTGTGGGCAAGTCATGAGCCTAGAAATG GCGTCTCACTTTTTAACAGTCCTAAAACAGAACAGCCATCTCCTGTAGTACACTCTTTCCCACACCCAGA GCTTCCTGAGGCGTTTGTTGGAACTCCAATTTCAAATACATCCCAGAGAATTTCTAGATTACTGGATTTG GTTGTCCATCCTGTACCCCAGCCTTCTCAGTGTTTGGAGTTTATTCAACAAAGTCCCACAAGATCTCCTT TGTGTCTGCTGTCCAGTTCGTTACCATTAAGTTCACAGTTTAAAAGGCCACATCAGAATACCTCCAGGCC TTCAGAGTTGCTTTTACTTGAGACTCCTCTCATAGTTAAGAAAGCTAAATCTTTGGCTCTGTCAGCCACG TCTTCTGGATTTGCCGAGTTTACTCCTCCATCCATCCTTAGGTCTGGTTTTCGAACAACACCTTTAGCAT CTCCCTCTTTGTCACCTGGAAGATCTCTCACTCCGCCTTTCAGAGTTAAAGAAACAAGGATTTCATTCAT GGAAGAAGGCATGAATACACACTGGACTGATAGAGCTACAGATGACCGAAATACAAAAGCGTTTGTTAGC ACATCTTTCCATAAATGTGGACTTCCAGCAGAAACTGAGTGGATGAAGACCAGTGATAAGAATACATATT TTCCTCTGGATGTCCCTGCAAAGGGCCCTCAGAAAGTGGTGGCAGAGTCACTGGCTACCCATTCAGGAAG GCTGGAGAAACTGGATGTGAGCAAAGAAGACAGCACAGCTTCCACCAGGTCAGACCAGACCTCCTTAGAG TATCATGACGCACCATCACCAGAAGACTTGGAAGGTGCTGTTTTTGTGTCTCCCAAGCCAGCATCTTCCT CCACTGAACTAACTACTAATTCAACTCTACAAACAGAGAGGGATAATGATAAAGATGCGTTTAAGTCAGA AGGTGCTCCTTCACCCGTGAAGAAACAAATAGGCACGGGAGATGCTGCAGTGGAAGCATTTTCAGAACTG AGTCGCTTAGACCCTGTTGAAAGAGCTGAAGCTTCTTTTGCTGTGTCGTCAGTCTGTGAAGGGGAAACCT CCACTTCAAACTCCAAGACGTCAGTTCTGGATGGAATCGTGCCTATTGAGAGCCGAACCTCCATACTTAC AGCAGACCACAAAGAGTCTGTGGCCAACACGGTTGCAGATGTTGAAAGCTCTGGGTCCACCAGCTCCAAG TGCCCGGTTACCTCTGAACGCAGCCTCGGCCAAAAACTAACATTAAACTTAAAAGAAGATGAAATAGAAG CTCATGTACCAAAGGAGAACGTTGGTTTACCAGAAGAAAGCCCTCGAATTTCTGCTGCTCCTTCTGATAC TCACGAGATTCATCTAATTGGATGTGAAAATCTTGAAGTTCAAAATTCAGAAGAGGAGGCCAAGAATCTT TCATTTGATGAGTTGTATCCCTTAGGGGCAGAGAAACTTGAGTATAATCTCAGTACTATTGAGCAGCAGT TTTGTGACTTGCCTGATGACAAAGACTCTGCTGAATGTGATGCTGCTGAAGTAGACGGGGAACTTTTTGT GGCCCAGAGCAACTTTACCCTGATTTTAGAAGGTGAAGAAGGAGAAGCTGAGGCAAGCGACTCTGCAGCA CCTAATATGTTACCGAAAGCGACCAAGGAAAAACCTGTGTGCCACAGGGAACCCCATAATCAGGAGCGCG TTACAGATTTGCCATCTGCTGTGACTGCTGACCAAGAATCCCACAAGGTAGAGACTTTACCGTATGTGCC TGAACCGGTTAAAGTGGCAATTGCAGAAAATCTGTTGGATGTAATTAAAGACACCAGAAGTAAGGAAGCA ACTCCCGTGGCAGCAGGTGAGGCTGGTGATGAGGACGGAGCAGTGATAGTCTCAAAGGCTGCACATTCGT CCAGGCTGACAAACTCTACACCGAAGACTGTTAAGGAACCACATGCAGAGACTGTAAATACCAGCCAGAA TGATGACATGGTTTCTTCTAGAACTCTCACAAGAAGGCAGCATGCCCTAAGCCTGAATGTCACATCAGAA CAAGAGCCTTCAGCAGTTGCCACTCCTAAGAAGAGAACTAGAAAAATTAAAGAAACTCCTGAGTCTTCTG AAAGGACCTGTTCTGACCTAAAAGTAGCACCTGAGAACCAACTGACAGCTCAGAGTCCTCCCGCTCCTAG GAGAGGAAAGAAGAAGGACGTTAGCCAAGGCACACTGCCAAGTTCTGGTGCTGTGGAGCCGGAGCCGGAA CCTCAGGGTACGCCGGGAAGACTGAGGCTGAGAACGCAGCCACCCGAGCCAGCAGCTGAAGAAACTCCTT CTAGAACAAAAGTCAGGCTTTCATCTGTTAGAAAGGGAACCCCTAGAAGACTTAAGAAGTCTGTAGAAAA TGGGCAAAGTACAGAAATTCTAGATGATCTCAAAGGGAGTGAGGCAGCAAGTCATGACGGGACTGTCACA GAGCTGAGGAATGCCAATTTAGAAGATACTCAGAATATGGAGTATAAACAAGATGAACACAGTGACCAGC AACTGCCTCTAAAACGAAAGAGGGTCAGAGAGAGAGAAGTTAGTGTGTCAAGTGTGACAGAAGAGCCAAA GCTTGACTCATCCCAGTTGCCTCTTCAGACAGGACTCGATGTACCTGCCACCCCTAGGAAACGTGGTAGA CCCAGGAAGGTAGTTCCCTTAGAAGCTGATGGTGGCACAACTGGTAAGGAACAGACAAGTCCTCAGAAGA AAGATGTTCCGGTTGTCCGGAGATCTACACGGAACACCCCAGCTAGAAATGTGAGTACTTTAGAAAAATC AGTTTTAGTGCCAAATAAGGAAGCTGCTCTAGTGGTGACATCTAAGAGGAGACCTACAAAGAAGTCTGCA GAGGAAAGCTCAAAAGATCCATCAGCGGCAGTCTCAGACCTGGCGGGTGGAGCAGCCCACACAGAGTCCG CTGACCGAAGGGACGGACTGCTTGCCGCCGCTGCTCTCACGCCATCTGCCCAGGGCACAAGGACTAGGTC TAGAAGGACCATGTTGTTGACGGACATTTCTGAACCCAAAACTGAGCCTTTATTTCCTCCTCCTTCAGTG AAGGTTCCAAAGAAAAAATCAAAAGCTGAGAACATGGAGGCCGCAGCCCAGCTGAAAGAATTGGTGTCAG ATTTATCTTCTCAGTTTGTTGTTTCCCCTCCTGCCTTGAGAACCAGGCAGAAAAGTATATCCAATACTTC CAAGCTTCTAGGTGAACTGGAGAGTGACCCTAAACCATTAGAGATCATAGAACAAAAACCAAAAAGAAGC AGGACTGTGAAGACAAGAGCAAGCAGAAACACAGGAAAAGGAAGTTCTTGGTCACCTCCTCCTGTAGAAA TTAAGCTGGTTTCTCCCTTGGCGAGTCCAGTGGATGAAATAAAGACCGGCAAGCCAAGAAAAACTGCAGA AATAGCAGGAAAAACTCTTGGAAGGGGCAGAAAGAAGCCATCTTCTTTTCCAAAGCAAATTTTACGCAGG AAAATGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR223137 representing NM_026375 Red=Cloning site Green=Tags(s) MQDLTAQVTSDLLHFPEVTIEALGEDEITLESVLRGKFAAGKNGLACLACGPQLEVVNSLTGERLSAYRF SGVNEQPPVVLAVKEFSWHKRTGLLIGLEEADGSVLCLYDLGISRVVKAVVLPGRVTAIEPIINHGGASA STQHLHPSLRWLFGVAAVVTDVGQILLIDLCLDDLSCSQNEVEASDLEVITGIPAEVPHIRERVMREGRH LCFQLVSPLGVAISTLSYINRTNQLAVGFSDGYLALWNMKSMKREYYTQLEGGRVPVHAVAFQEPENDPR NCCYLWAVQSTQDSEGDVLSLHLLQLAFGDRKCLASGQILYEGLEYCEERYTLDLAGGTFPLRGQTSNTK LLGCQSIERFPSHGDREESMREALSPDTSVSVFTWQVNIYGQGKPSVYLGLFDINRWYHAQMPDSLRSGE SLHNCSYFALWSLDSVVSRTSPHHILDILVHERSLNRGVPPSYPPPEQFFNPSTFNFDATCLLDSGVIHV TCAGFQKETLTFLKKSGPTLNEVIPDSYNRCLVAGLLSPRLIDIQPSSLSQEEQLEAILSAAIQTSSLGL LTGYIRTWIIEEQPNSAANLRFVLEWTWNKVVLTKEEFDRLCVPLFDGSCRFIDPQTIQSIQQCHLLLSN LSTVLSCFAMEAQGITERGLVDLSNKHMVTQLLCQYAHMVLWFCHSGLLPEGLDDALQLSRLRYNYPVIQ NYYTSRRQKSERSPRGKWNHDCLMIDGLVSQLGDEVEKLWKRDEGGTGRYPPASIHALLDIYLLDNITEA SKHAITIYLLLDIMYSFPNKTDTPIESFPTAFAISWGQVKLVQGFWLLDHNDYENGLDLLFHPVTAKPAS WQHSKIIEAFMSQGEHKQALRYLQTMKPTVSSSNEVILHLTVLLFNRCMVEAWNLLRQNSNRVNIEELLK HAYEVCQEMGLMEDLLKLPFTNTEQECLVKFLQSSTSVENHEFLLVHHLQRANYISALKLNQILKNNLMS DRDPRLRERSVTRNSILDQYGKILPRVQRKLAVERAKPYHLSTSSVFHEVSRPKPLSAFPKKAITGTVLT RSTFISNVLSKIGEVWASHEPRNGVSLFNSPKTEQPSPVVHSFPHPELPEAFVGTPISNTSQRISRLLDL VVHPVPQPSQCLEFIQQSPTRSPLCLLSSSLPLSSQFKRPHQNTSRPSELLLLETPLIVKKAKSLALSAT SSGFAEFTPPSILRSGFRTTPLASPSLSPGRSLTPPFRVKETRISFMEEGMNTHWTDRATDDRNTKAFVS TSFHKCGLPAETEWMKTSDKNTYFPLDVPAKGPQKVVAESLATHSGRLEKLDVSKEDSTASTRSDQTSLE YHDAPSPEDLEGAVFVSPKPASSSTELTTNSTLQTERDNDKDAFKSEGAPSPVKKQIGTGDAAVEAFSEL SRLDPVERAEASFAVSSVCEGETSTSNSKTSVLDGIVPIESRTSILTADHKESVANTVADVESSGSTSSK CPVTSERSLGQKLTLNLKEDEIEAHVPKENVGLPEESPRISAAPSDTHEIHLIGCENLEVQNSEEEAKNL SFDELYPLGAEKLEYNLSTIEQQFCDLPDDKDSAECDAAEVDGELFVAQSNFTLILEGEEGEAEASDSAA PNMLPKATKEKPVCHREPHNQERVTDLPSAVTADQESHKVETLPYVPEPVKVAIAENLLDVIKDTRSKEA TPVAAGEAGDEDGAVIVSKAAHSSRLTNSTPKTVKEPHAETVNTSQNDDMVSSRTLTRRQHALSLNVTSE QEPSAVATPKKRTRKIKETPESSERTCSDLKVAPENQLTAQSPPAPRRGKKKDVSQGTLPSSGAVEPEPE PQGTPGRLRLRTQPPEPAAEETPSRTKVRLSSVRKGTPRRLKKSVENGQSTEILDDLKGSEAASHDGTVT ELRNANLEDTQNMEYKQDEHSDQQLPLKRKRVREREVSVSSVTEEPKLDSSQLPLQTGLDVPATPRKRGR PRKVVPLEADGGTTGKEQTSPQKKDVPVVRRSTRNTPARNVSTLEKSVLVPNKEAALVVTSKRRPTKKSA EESSKDPSAAVSDLAGGAAHTESADRRDGLLAAAALTPSAQGTRTRSRRTMLLTDISEPKTEPLFPPPSV KVPKKKSKAENMEAAAQLKELVSDLSSQFVVSPPALRTRQKSISNTSKLLGELESDPKPLEIIEQKPKRS RTVKTRASRNTGKGSSWSPPPVEIKLVSPLASPVDEIKTGKPRKTAEIAGKTLGRGRKKPSSFPKQILRR KML TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_026375
ORF Size	6729 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_026375.2, NP_080651.2
RefSeq Size	8831 bp
RefSeq ORF	6732 bp
Locus ID	226747
UniProt ID	Q8CJF7
MW	247.6 kDa
Gene Summary	Required for the assembly of a functional nuclear pore complex (NPC) on the surface of chromosomes as nuclei form at the end of mitosis. May initiate NPC assembly by binding to chromatin and recruiting the Nup107-160 subcomplex of the NPC. Also required for the localization of the Nup107-160 subcomplex of the NPC to the kinetochore during mitosis and for the completion of cytokinesis (By similarity). Has also been proposed to function as a transcription factor which may play a specific role in hematopoietic tissues (PubMed:11952839).[UniProtKB/Swiss-Prot Function]

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