Ahctf1 (NM_026375) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6230412P20Rik; AV011447; Elys
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225241 representing NM_026375 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAGACTTGACAGCTCAAGTGACTAGTGATCTCCTGCATTTCCCAGAAGTGACTATTGAAGCTCTTG GAGAAGATGAGATAACATTAGAGTCCGTGCTTCGTGGAAAGTTTGCTGCAGGGAAAAATGGACTAGCATG CTTAGCTTGTGGTCCACAACTTGAAGTTGTAAACTCCCTAACAGGAGAGCGGTTATCTGCATATAGATTC AGTGGAGTAAATGAACAGCCTCCTGTAGTCCTTGCAGTGAAAGAATTCTCTTGGCATAAGAGGACTGGAT TGTTAATAGGATTGGAAGAAGCAGATGGGAGTGTTCTTTGTCTTTATGACCTTGGTATATCAAGAGTGGT CAAAGCAGTTGTTCTTCCTGGAAGGGTAACAGCTATCGAGCCTATAATTAACCATGGAGGAGCCAGTGCG AGTACCCAGCATTTACATCCAAGTCTCCGGTGGCTTTTTGGCGTGGCCGCTGTGGTGACTGATGTTGGAC AGATCCTTCTTATTGACCTGTGTTTGGATGACTTGTCCTGCAGTCAGAATGAAGTTGAGGCATCAGACCT TGAAGTTATCACTGGTATCCCAGCTGAAGTACCACACATCAGAGAGAGAGTGATGAGAGAGGGGCGCCAC CTGTGCTTCCAGTTAGTAAGCCCATTGGGAGTAGCCATTTCTACTCTGAGTTACATCAACAGGACAAATC AGCTTGCTGTGGGTTTTTCTGATGGCTACTTAGCACTTTGGAACATGAAAAGCATGAAAAGAGAATACTA TACACAGTTGGAAGGTGGAAGGGTTCCTGTCCATGCAGTTGCCTTTCAAGAGCCTGAGAATGATCCTCGT AACTGCTGTTATTTATGGGCTGTTCAGTCCACACAAGATAGTGAAGGGGATGTTTTGAGTTTGCATCTGC TTCAGCTGGCTTTTGGTGATAGAAAATGTTTGGCATCAGGGCAAATTTTATATGAGGGATTAGAATACTG CGAAGAAAGATATACACTGGATCTAGCAGGTGGCACGTTCCCCTTAAGGGGACAAACTAGTAATACCAAA TTGTTGGGATGCCAGAGTATAGAGAGATTTCCATCTCATGGAGACAGAGAAGAAAGTATGAGAGAAGCTC TGTCTCCCGATACCAGCGTTTCTGTCTTTACCTGGCAAGTGAATATATATGGACAGGGAAAGCCTTCTGT GTATTTAGGGCTATTTGACATAAATCGTTGGTATCATGCACAAATGCCCGATTCTTTAAGATCAGGAGAA TCTCTGCATAATTGCTCTTATTTTGCGTTGTGGTCATTGGATTCGGTTGTAAGTAGGACTTCTCCACATC ACATCTTGGACATACTAGTACATGAGAGGAGTTTAAACCGAGGGGTTCCTCCTTCCTACCCACCTCCAGA GCAATTTTTTAACCCAAGTACTTTTAATTTTGATGCCACTTGTTTGTTAGACTCTGGAGTTATCCATGTA ACTTGTGCTGGATTTCAGAAGGAGACTTTGACATTTTTAAAGAAATCAGGACCAACTCTTAATGAAGTCA TTCCTGATAGTTATAATCGATGTCTTGTTGCTGGTCTCCTCTCACCAAGACTTATTGATATTCAGCCTTC CAGTTTAAGTCAAGAAGAACAATTAGAAGCTATATTGTCAGCAGCAATTCAGACAAGTTCCTTGGGACTT TTGACTGGTTACATCAGAACGTGGATAATAGAAGAACAACCAAATTCTGCTGCTAATCTACGATTTGTTC TTGAGTGGACATGGAATAAAGTGGTTCTCACAAAAGAAGAGTTTGATAGGCTTTGTGTGCCGCTGTTTGA CGGTTCGTGTCGTTTTATTGACCCACAGACTATTCAGTCTATCCAGCAGTGCCATTTACTGCTTAGCAAC CTTAGTACAGTCTTAAGCTGTTTTGCAATGGAGGCCCAGGGTATCACTGAGAGAGGACTGGTGGACTTGA GCAACAAGCACATGGTCACCCAGCTTCTCTGTCAGTACGCACACATGGTTCTGTGGTTCTGCCACTCGGG GCTTCTGCCCGAAGGCTTAGATGATGCTCTGCAGCTGTCAAGACTACGCTACAACTACCCTGTAATTCAG AACTACTATACAAGTCGTCGGCAGAAGTCTGAGCGCTCACCCAGAGGGAAGTGGAACCACGACTGCTTGA TGATTGATGGATTAGTCTCTCAACTAGGAGATGAAGTTGAGAAGTTGTGGAAGCGGGACGAAGGTGGCAC AGGAAGATACCCTCCTGCTAGCATCCACGCATTACTTGATATATATTTATTAGACAACATTACCGAAGCA AGCAAACATGCTATTACCATTTATTTGCTGCTTGATATTATGTATTCCTTTCCAAATAAAACGGATACCC CCATTGAATCTTTCCCAACTGCCTTTGCTATTTCTTGGGGCCAAGTTAAGCTAGTTCAAGGATTTTGGCT GCTAGATCATAATGACTATGAGAATGGTTTAGACCTTCTGTTTCACCCAGTTACTGCAAAGCCTGCATCG TGGCAACATTCAAAGATAATTGAAGCTTTTATGAGTCAGGGAGAGCACAAACAGGCTCTCCGGTATCTTC AGACAATGAAGCCAACAGTGTCCAGTAGCAATGAAGTTATCCTTCACCTCACTGTTCTACTTTTTAATAG ATGCATGGTTGAGGCCTGGAACTTACTGCGACAGAATTCAAACAGAGTAAATATAGAGGAATTATTAAAG CACGCTTATGAAGTTTGTCAGGAGATGGGCTTAATGGAGGATTTACTGAAGCTGCCATTTACAAACACTG AGCAGGAATGCTTAGTGAAATTTTTACAGTCCAGTACCAGTGTTGAGAATCATGAATTCCTTCTAGTTCA CCATTTACAGCGTGCCAATTATATTTCTGCCTTGAAACTAAACCAGATTCTGAAGAATAATCTCATGAGT GATCGTGACCCTCGATTGCGGGAAAGATCGGTGACTCGAAATTCTATATTAGACCAGTATGGGAAAATCC TACCTAGAGTCCAGAGAAAGTTAGCTGTTGAGCGAGCTAAGCCTTACCACCTGTCGACATCCTCAGTTTT TCATGAAGTTTCTAGACCCAAACCGTTATCGGCATTTCCAAAGAAAGCTATAACTGGAACAGTGTTAACC CGATCTACGTTCATCAGCAATGTTTTATCTAAAATTGGAGAGGTGTGGGCAAGTCATGAGCCTAGAAATG GCGTCTCACTTTTTAACAGTCCTAAAACAGAACAGCCATCTCCTGTAGTACACTCTTTCCCACACCCAGA GCTTCCTGAGGCGTTTGTTGGAACTCCAATTTCAAATACATCCCAGAGAATTTCTAGATTACTGGATTTG GTTGTCCATCCTGTACCCCAGCCTTCTCAGTGTTTGGAGTTTATTCAACAAAGTCCCACAAGATCTCCTT TGTGTCTGCTGTCCAGTTCGTTACCATTAAGTTCACAGTTTAAAAGGCCACATCAGAATACCTCCAGGCC TTCAGAGTTGCTTTTACTTGAGACTCCTCTCATAGTTAAGAAAGCTAAATCTTTGGCTCTGTCAGCCACG TCTTCTGGATTTGCCGAGTTTACTCCTCCATCCATCCTTAGGTCTGGTTTTCGAACAACACCTTTAGCAT CTCCCTCTTTGTCACCTGGAAGATCTCTCACTCCGCCTTTCAGAGTTAAAGAAACAAGGATTTCATTCAT GGAAGAAGGCATGAATACACACTGGACTGATAGAGCTACAGATGACCGAAATACAAAAGCGTTTGTTAGC ACATCTTTCCATAAATGTGGACTTCCAGCAGAAACTGAGTGGATGAAGACCAGTGATAAGAATACATATT TTCCTCTGGATGTCCCTGCAAAGGGCCCTCAGAAAGTGGTGGCAGAGTCACTGGCTACCCATTCAGGAAG GCTGGAGAAACTGGATGTGAGCAAAGAAGACAGCACAGCTTCCACCAGGTCAGACCAGACCTCCTTAGAG TATCATGACGCACCATCACCAGAAGACTTGGAAGGTGCTGTTTTTGTGTCTCCCAAGCCAGCATCTTCCT CCACTGAACTAACTACTAATTCAACTCTACAAACAGAGAGGGATAATGATAAAGATGCGTTTAAGTCAGA AGGTGCTCCTTCACCCGTGAAGAAACAAATAGGCACGGGAGATGCTGCAGTGGAAGCATTTTCAGAACTG AGTCGCTTAGACCCTGTTGAAAGAGCTGAAGCTTCTTTTGCTGTGTCGTCAGTCTGTGAAGGGGAAACCT CCACTTCAAACTCCAAGACGTCAGTTCTGGATGGAATCGTGCCTATTGAGAGCCGAACCTCCATACTTAC AGCAGACCACAAAGAGTCTGTGGCCAACACGGTTGCAGATGTTGAAAGCTCTGGGTCCACCAGCTCCAAG TGCCCGGTTACCTCTGAACGCAGCCTCGGCCAAAAACTAACATTAAACTTAAAAGAAGATGAAATAGAAG CTCATGTACCAAAGGAGAACGTTGGTTTACCAGAAGAAAGCCCTCGAATTTCTGCTGCTCCTTCTGATAC TCACGAGATTCATCTAATTGGATGTGAAAATCTTGAAGTTCAAAATTCAGAAGAGGAGGCCAAGAATCTT TCATTTGATGAGTTGTATCCCTTAGGGGCAGAGAAACTTGAGTATAATCTCAGTACTATTGAGCAGCAGT TTTGTGACTTGCCTGATGACAAAGACTCTGCTGAATGTGATGCTGCTGAAGTAGACGGGGAACTTTTTGT GGCCCAGAGCAACTTTACCCTGATTTTAGAAGGTGAAGAAGGAGAAGCTGAGGCAAGCGACTCTGCAGCA CCTAATATGTTACCGAAAGCGACCAAGGAAAAACCTGTGTGCCACAGGGAACCCCATAATCAGGAGCGCG TTACAGATTTGCCATCTGCTGTGACTGCTGACCAAGAATCCCACAAGGTAGAGACTTTACCGTATGTGCC TGAACCGGTTAAAGTGGCAATTGCAGAAAATCTGTTGGATGTAATTAAAGACACCAGAAGTAAGGAAGCA ACTCCCGTGGCAGCAGGTGAGGCTGGTGATGAGGACGGAGCAGTGATAGTCTCAAAGGCTGCACATTCGT CCAGGCTGACAAACTCTACACCGAAGACTGTTAAGGAACCACATGCAGAGACTGTAAATACCAGCCAGAA TGATGACATGGTTTCTTCTAGAACTCTCACAAGAAGGCAGCATGCCCTAAGCCTGAATGTCACATCAGAA CAAGAGCCTTCAGCAGTTGCCACTCCTAAGAAGAGAACTAGAAAAATTAAAGAAACTCCTGAGTCTTCTG AAAGGACCTGTTCTGACCTAAAAGTAGCACCTGAGAACCAACTGACAGCTCAGAGTCCTCCCGCTCCTAG GAGAGGAAAGAAGAAGGACGTTAGCCAAGGCACACTGCCAAGTTCTGGTGCTGTGGAGCCGGAGCCGGAA CCTCAGGGTACGCCGGGAAGACTGAGGCTGAGAACGCAGCCACCCGAGCCAGCAGCTGAAGAAACTCCTT CTAGAACAAAAGTCAGGCTTTCATCTGTTAGAAAGGGAACCCCTAGAAGACTTAAGAAGTCTGTAGAAAA TGGGCAAAGTACAGAAATTCTAGATGATCTCAAAGGGAGTGAGGCAGCAAGTCATGACGGGACTGTCACA GAGCTGAGGAATGCCAATTTAGAAGATACTCAGAATATGGAGTATAAACAAGATGAACACAGTGACCAGC AACTGCCTCTAAAACGAAAGAGGGTCAGAGAGAGAGAAGTTAGTGTGTCAAGTGTGACAGAAGAGCCAAA GCTTGACTCATCCCAGTTGCCTCTTCAGACAGGACTCGATGTACCTGCCACCCCTAGGAAACGTGGTAGA CCCAGGAAGGTAGTTCCCTTAGAAGCTGATGGTGGCACAACTGGTAAGGAACAGACAAGTCCTCAGAAGA AAGATGTTCCGGTTGTCCGGAGATCTACACGGAACACCCCAGCTAGAAATGTGAGTACTTTAGAAAAATC AGTTTTAGTGCCAAATAAGGAAGCTGCTCTAGTGGTGACATCTAAGAGGAGACCTACAAAGAAGTCTGCA GAGGAAAGCTCAAAAGATCCATCAGCGGCAGTCTCAGACCTGGCGGGTGGAGCAGCCCACACAGAGTCCG CTGACCGAAGGGACGGACTGCTTGCCGCCGCTGCTCTCACGCCATCTGCCCAGGGCACAAGGACTAGGTC TAGAAGGACCATGTTGTTGACGGACATTTCTGAACCCAAAACTGAGCCTTTATTTCCTCCTCCTTCAGTG AAGGTTCCAAAGAAAAAATCAAAAGCTGAGAACATGGAGGCCGCAGCCCAGCTGAAAGAATTGGTGTCAG ATTTATCTTCTCAGTTTGTTGTTTCCCCTCCTGCCTTGAGAACCAGGCAGAAAAGTATATCCAATACTTC CAAGCTTCTAGGTGAACTGGAGAGTGACCCTAAACCATTAGAGATCATAGAACAAAAACCAAAAAGAAGC AGGACTGTGAAGACAAGAGCAAGCAGAAACACAGGAAAAGGAAGTTCTTGGTCACCTCCTCCTGTAGAAA TTAAGCTGGTTTCTCCCTTGGCGAGTCCAGTGGATGAAATAAAGACCGGCAAGCCAAGAAAAACTGCAGA AATAGCAGGAAAAACTCTTGGAAGGGGCAGAAAGAAGCCATCTTCTTTTCCAAAGCAAATTTTACGCAGG AAAATGCTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_026375
Insert Size	6732 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_026375.2, NP_080651.2
RefSeq Size	8831 bp
RefSeq ORF	6732 bp
Locus ID	226747
UniProt ID	Q8CJF7
Gene Summary	Required for the assembly of a functional nuclear pore complex (NPC) on the surface of chromosomes as nuclei form at the end of mitosis. May initiate NPC assembly by binding to chromatin and recruiting the Nup107-160 subcomplex of the NPC. Also required for the localization of the Nup107-160 subcomplex of the NPC to the kinetochore during mitosis and for the completion of cytokinesis (By similarity). Has also been proposed to function as a transcription factor which may play a specific role in hematopoietic tissues (PubMed:11952839).[UniProtKB/Swiss-Prot Function]

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