Sts (NM_009293) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ArsC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR223101 representing NM_009293, codon optimized. Due to the complexity of NM_009293, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCGGCCCAGACCCTTGCTGCTGGCGGTGATGGCCGCTACTTTGGCCGATATAATCCTGGCTGCCG ATCCCGCGCCCGCCGGACCCGCTCCGAGGCCTCCCAACTTCCTTTTGATCATGGCAGATGATCTTGGAAT CGGAGATCTTGGCTGCTACGGAAACAAAACGTTGCGGACTCCACACCTGGACCGCCTGGCTAGGGAGGGA GTGAAACTCACTCAACACCTGGCTGCTGCTCCCCTTTGTACTCCTTCCCGGGCCGCCTTCCTGACCGGCC GCTACCCACCACGGTCAGGCATGGCTGCCCACGGCCGGGTCGGTGTATACTTGTTTACCGCGTCTAGCGG GGGGCTCCCTCCATCCGAAGTCACCATGGCACGCCTGCTGAAGGGACGGGGCTATGCTACCGCTCTTATC GGCAAATGGCACCTGGGGCTGTCCTGTAGGGGCGCCACCGACTTTTGTCACCATCCGCTGAGGCATGGCT TTGATAGGTTCCTCGGAGTGCCAACTACCAACTTGCGCGACTGCAGACCTGGCGCCGGGACCGTATTTGG CCCTGCTCTCCGGGTGTTTGCGGCAGGCCCACTGGCTGCCCTCGGGGCATCACTTGCAGCTATGGCCGCT GCCAGATGGGCAGGGCTTGCTAGAGTGCCAGGGTGGGCTTTGGCTGGTACTGCCGCCGCAATGCTGGCCG TCGGGGGCCCTCGGTCCGCTAGTTGCTTGGGGTTTAGGCCAGCAAACTGCTTCCTCATGGATGATCTGGC TGTTGCACAAAGGCCAACCGACTATGGCGGCCTCACCAGACGGCTGGCTGACGAGGCCGCCCTGTTTCTT CGGCGCAATCGCGCAAGACCGTTCCTCCTTTTCCTGAGCTTCCTGCACGTTCACACAGCACACTTCGCCG ACCCTGGGTTCGCAGGGCGCTCTCTGCACGGGGCCTACGGGGATTCTGTAGAAGAGATGGACTGGGGTGT GGGTAGGGTCCTCGCTGCCCTGGATGAGCTGGGACTGGCTAGAGAAACCCTGGTTTACTTTACTTCAGAC CACGGGGCACACGTGGAGGAGCTGGGGCCCAGAGGAGAGCGAATGGGAGGCTCCAACGGTGTCTTCCGAG GCGGCAAGGGAAATAACTGGGAAGGCGGCGTCCGCGTGCCTTGCCTGGTCAGATGGCCTCGCGAACTCAG TCCTGGACGGGTCGTTGCCGAACCCACCAGCTTGATGGATGTCTTCCCCACAGTAGCCAGACTCGCCGGA GCCGAACTGCCAGGCGACAGGGTTATCGATGGTCGCGACCTGATGCCTTTGCTTCGCGGCGATGCGCAGA GGAGCGAGCATGAATTCCTTTTTCACTACTGTAACGCCTACTTGCAGGCAGTCCGCTGGCACAATGGAAG CGCTGTGTGGAAGGCCTTTTACTTCACCCCTAATTTCGCCCCCGCTGGCGCCAACGGGTGTTTCAGCACT CACGTCTGCCTTTGCGCCGGTCCTGCCCACGTTACTGCACACGACCCTCCCCTGCTGTTCGACCTGACTC GGGATCCTGGAGAAAGGCGACCTCTTACGCCAGAAGCCGAGCCGCGGCACCGAGAGGTCCTCGATGCAAT CGACGCAGCAGCCAGAGCTCACCGAGCACGCCTGCGGCCAGCCCCCGACCAGCTGGCTCCTCGCCACCTT ATGTGGAAACCATGGCTGCAGCTGTGGGGTGGAGGGGGTGCTGGGGGTGGCGCCGGTGCACAGGACGATT CCGGGCACGCCCACGGCGACGGCAGCCATGCCCATGACGACCCTGGTCACGCCCAAGACCGGGGTGACGA TGATGCACATTATGGTGGCCACGCGACCACTCGCACACAGGCCACCCCACGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR223101 representing NM_009293 Red=Cloning site Green=Tags(s) MPRPRPLLLAVMAATLADIILAADPAPAGPAPRPPNFLLIMADDLGIGDLGCYGNKTLRTPHLDRLAREG VKLTQHLAAAPLCTPSRAAFLTGRYPPRSGMAAHGRVGVYLFTASSGGLPPSEVTMARLLKGRGYATALI GKWHLGLSCRGATDFCHHPLRHGFDRFLGVPTTNLRDCRPGAGTVFGPALRVFAAGPLAALGASLAAMAA ARWAGLARVPGWALAGTAAAMLAVGGPRSASCLGFRPANCFLMDDLAVAQRPTDYGGLTRRLADEAALFL RRNRARPFLLFLSFLHVHTAHFADPGFAGRSLHGAYGDSVEEMDWGVGRVLAALDELGLARETLVYFTSD HGAHVEELGPRGERMGGSNGVFRGGKGNNWEGGVRVPCLVRWPRELSPGRVVAEPTSLMDVFPTVARLAG AELPGDRVIDGRDLMPLLRGDAQRSEHEFLFHYCNAYLQAVRWHNGSAVWKAFYFTPNFAPAGANGCFST HVCLCAGPAHVTAHDPPLLFDLTRDPGERRPLTPEAEPRHREVLDAIDAAARAHRARLRPAPDQLAPRHL MWKPWLQLWGGGGAGGGAGAQDDSGHAHGDGSHAHDDPGHAQDRGDDDAHYGGHATTRTQATPR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_009293
ORF Size	1872 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_009293.1, NP_033319.1
RefSeq Size	2283 bp
RefSeq ORF	1875 bp
Locus ID	20905
UniProt ID	P50427
MW	66.6 kDa
Gene Summary	Catalyzes the conversion of sulfated steroid precursors, such as dehydroepiandrosterone sulfate (DHEA-S) and estrone sulfate to the free steroid.[UniProtKB/Swiss-Prot Function]

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