Sts (NM_009293) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	ArsC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC219810 representing NM_009293 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAGGCCCCGCCCCCTGCTCCTCGCCGTCATGGCCGCCACGCTCGCTGACATCATCCTCGCCGCGG ATCCCGCCCCCGCAGGCCCCGCCCCCCGGCCGCCCAACTTCCTGCTCATCATGGCCGACGACCTGGGCAT CGGTGACCTCGGCTGCTACGGCAACAAAACGCTCAGGACGCCGCACCTCGACCGCCTGGCCCGCGAGGGG GTGAAGCTGACGCAGCACCTGGCGGCCGCCCCGCTCTGCACCCCCAGCCGCGCCGCCTTCCTGACCGGGC GCTACCCGCCGCGCTCAGGCATGGCGGCGCACGGCCGCGTGGGCGTGTACCTGTTCACGGCGTCGTCGGG GGGCCTCCCGCCCAGCGAGGTCACCATGGCGCGGCTGCTGAAGGGGCGGGGCTACGCCACGGCGCTCATT GGGAAGTGGCACCTGGGTCTGAGCTGCCGCGGGGCCACCGACTTCTGCCACCACCCCCTGCGCCACGGCT TCGACCGCTTCCTGGGCGTCCCGACCACCAACCTGCGCGACTGCCGGCCGGGGGCGGGCACCGTGTTCGG CCCCGCCCTGCGCGTGTTCGCGGCCGGGCCCCTGGCGGCGCTGGGGGCGTCGCTGGCGGCCATGGCGGCG GCGCGGTGGGCGGGGCTGGCGCGGGTGCCGGGCTGGGCCCTGGCGGGGACGGCGGCGGCCATGTTGGCGG TGGGCGGGCCGCGCTCGGCTTCCTGCCTCGGCTTCCGCCCGGCCAACTGCTTCCTCATGGACGACCTCGC CGTGGCGCAGCGGCCCACGGACTACGGCGGACTCACGCGGCGCCTGGCGGACGAGGCCGCGCTCTTCCTG CGGAGGAACCGGGCCCGCCCCTTCCTGCTCTTCCTGTCCTTCCTGCACGTCCACACCGCGCACTTCGCCG ACCCCGGGTTCGCGGGGCGGAGCCTGCACGGGGCCTACGGCGACAGCGTGGAGGAGATGGACTGGGGCGT GGGCCGCGTGCTGGCGGCGCTGGACGAGCTGGGCCTGGCGCGCGAGACGCTCGTCTACTTCACCTCGGAC CACGGGGCCCACGTGGAGGAGCTGGGGCCGCGCGGCGAGCGCATGGGCGGCAGCAACGGCGTCTTCCGCG GCGGGAAGGGCAACAACTGGGAGGGCGGCGTCCGCGTCCCCTGCCTCGTGCGTTGGCCCCGCGAGCTCAG CCCCGGCCGCGTCGTCGCGGAGCCCACGAGCCTCATGGACGTCTTCCCCACCGTGGCGCGGCTGGCGGGG GCGGAGCTGCCCGGGGACAGGGTGATTGACGGCCGGGACCTGATGCCGCTGCTGCGCGGCGACGCCCAGC GCTCGGAGCACGAGTTCCTGTTCCACTACTGCAACGCCTACCTGCAGGCCGTGCGCTGGCACAACGGCAG CGCGGTGTGGAAGGCCTTCTACTTCACGCCCAACTTCGCGCCCGCGGGCGCCAACGGCTGCTTCTCGACC CACGTCTGCCTCTGCGCCGGCCCCGCCCACGTCACCGCCCACGACCCCCCCCTGCTGTTCGACCTCACGC GCGACCCCGGCGAGCGCCGCCCCCTGACCCCCGAGGCCGAGCCCCGGCACCGCGAGGTCCTGGACGCCAT CGACGCCGCCGCCCGCGCCCACCGCGCCCGGCTCCGCCCCGCGCCCGACCAGCTCGCCCCGCGGCACCTG ATGTGGAAGCCGTGGCTGCAGCTGTGGGGCGGGGGCGGGGCCGGGGGCGGGGCCGGCGCCCAGGACGACT CAGGCCACGCCCACGGCGATGGAAGCCACGCCCACGACGACCCAGGCCACGCCCAGGACAGAGGCGATGA CGACGCCCACTACGGAGGCCACGCCACGACACGGACTCAGGCCACGCCACGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_009293
Insert Size	1875 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_009293.1, NP_033319.1
RefSeq Size	2283 bp
RefSeq ORF	1875 bp
Locus ID	20905
UniProt ID	P50427
Gene Summary	Catalyzes the conversion of sulfated steroid precursors, such as dehydroepiandrosterone sulfate (DHEA-S) and estrone sulfate to the free steroid.[UniProtKB/Swiss-Prot Function]

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