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Cyfip1 (NM_001164661) Mouse Tagged ORF Clone
CAT＃: MR222940
- TrueORF®
Cyfip1 (Myc-DDK-tagged) - Mouse cytoplasmic FMR1 interacting protein 1 (Cyfip1), transcript variant 1
ORF Plasmid: tGFP
"NM_001164661" in other vectors (3)
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CNY 12,540.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | E030028J09Rik; l(7)1Rl; l7Rl1; l71Rl; mKIAA0068; P140SRA-1; P140sra1; pl-1; Shyc; Sra-1; Sra1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222940 representing NM_001164661
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCCAAGTGACACTAGAGGACGCACTGTCCAATGTGGACCTCCTGGAGGAGCTGCCTCTGCCTG ACCAGCAGCCCTGCATCGAGCCCCCACCGTCCTCGCTGCTCTATCAGCCAAATTTCAACACCAACTTTGA AGACAGAAATGCATTTGTCACTGGCATTGCAAGATACATCGAACAAGCAACTGTCCACTCTAGCATGAAC GAGATGCTAGAGGAAGGCCAAGAGTATGCTGTCATGCTGTACACCTGGAGGAGCTGCTCCCGGGCCATCC CTCAGGTGAAGTGCAACGAGCAGCCGAATAGAGTTGAAATTTATGAGAAAACCGTGGAAGTCCTTGAACC CGAGGTCACAAAACTGATGAATTTTATGTACTTTCAGAGAAATGCCATCGAGCGGTTCTGTGGGGAAGTG AGACGCCTGTGTCATGCTGAGAGGAGAAAGGACTTTGTTTCTGAAGCCTACTTGATCACCCTGGGCAAAT TTATCAACATGTTTGCTGTGTTGGATGAGCTGAAGAACATGAAGTGCAGTGTGAAGAATGACCACTCTGC ATATAAGAGGGCTGCTCAGTTTTTACGTAAAATGGCAGATCCACAATCCATCCAGGAGTCACAGAATCTG TCCATGTTCCTGGCCAACCACAACAAGATTACACAATCTCTGCAGCAGCAGCTTGAGGTCATTTCTGGCT ATGAAGAGCTCCTGGCAGACATCGTGAATCTGTGTGTGGATTACTATGAGAACAGGATGTACTTGACACC CAGCGAAAAACACATGCTTCTCAAAGTCATGGGATTTGGCCTGTACCTGATGGATGGGAGTGTGAGTAAC ATCTACAAACTAGATGCCAAGAAAAGAATAAATTTATCCAAAATCGACAAGTATTTCAAGCAGCTGCAGG TGGTTCCACTCTTTGGAGATATGCAAATAGAACTGGCAAGATACATCAAGACCAGCGCACACTATGAGGA GAATAAGTCCCGGTGGACCTGCGCGTCCTCCAGCAGCAGCCCGCAGTACAACATCTGTGAGCAGATGATC CAGATCCGTGAGGACCATATGCGTTTCATCTCCGAGCTGGCACGCTACAGCAATAGCGAGGTAGTCACAG GGTCTGGCCGGCAGGAAGCCCAGAAGACAGATGCTGAGTACCGGAAGCTCTTCGATCTGGCTCTGCAGGG CCTGCAGCTTCTGTCGCAGTGGAGCGCTCACGTGATGGAAGTGTATTCCTGGAAACTTGTACATCCAACA GACAAGTACTCCAACAAGGACTGCCCCGACAACGCTGAGGAGTACGAGCGAGCCACACGCTACAACTACA CCACTGAGGAGAAGTTTGCCCTGGTGGAGGTGATTGCCATGATCAAAGGCCTGCAGGTGCTGATGGGCAG GATGGAGAGTGTGTTCAATCACGCCATCAGACACACGGTTTACGCTGCGCTTCAGGACTTCTCCCAGGTG ACCCTCAGGGAACCACTGCGACAGGCCATCAAGAAGAAAAAGAACGTAATCCAGAGTGTCCTACAGGCCA TCAGGAAGACTGTGTGTGACTGGGAGACTGGGCATGAGCCCTTTAACGACCCAGCTCTGCGGGGAGAGAA GGACCCTAAGAGTGGCTTTGACATCAAGGTGCCACGCCGTGCTGTGGGACCCTCCAGCACGCAGCTTTAC ATGGTGAGAACTATGCTAGAGTCCCTCATTGCAGACAAAAGTGGTTCCAAGAAAACCTTGAGAAGTAGCC TTGAGGGGCCCACCATATTGGACATAGAAAAATTTCATCGAGAATCATTCTTCTACACTCACTTGATAAA TTTCAGTGAAACACTGCAGCAGTGCTGTGACCTTTCCCAGCTGTGGTTCCGAGAGTTCTTCCTGGAGCTG ACCATGGGCAGGAGGATCCAGTTCCCCATTGAGATGTCTATGCCCTGGATTCTGACTGACCACATCCTAG AGACCAAGGAGGCATCAATGATGGAGTACGTCCTCTACTCCTTGGACCTGTACAACGACAGTGCCCACTA TGCGCTCACCAAGTTCAACAAGCAGTTTCTCTATGATGAGATTGAGGCAGAGGTGAATCTGTGTTTTGAC CAATTTGTTTATAAACTAGCAGACCAGATATTTGCATATTACAAGGTTATGGCAGGAAGTTTGCTTCTTG ACAAAAGGTTACGGTCAGAATGCAAAAATCAGGGAGCCACGATCCACCTCCCCCCATCTAACCGCTATGA GACGCTGTTGAAGCAAAGGCACGTGCAGCTGCTCGGGAGATCCATTGATCTCAACCGTCTGATTACACAG CGCGTTTCAGCAGCCATGTACAAATCTCTAGAACTGGCAATTGGACGGTTTGAAAGTGAAGATTTAACAT CAGTAGTCGAGCTAGATGGACTCTTAGAAATCAACCGTATGACACACAAGCTGTTGAGCAGGTACCTCAC ACTGGACAGCTTTGATGCCATGTTCCGGGAAGCCAACCACAACGTGTCTGCACCTTATGGAAGAATTACC CTCCACGTCTTCTGGGAGCTAAACTATGACTTCTTGCCCAACTACTGCTATAATGGCTCTACCAACCGGT TTGTTCGGACAGTATTACCATTTTCTCAGGAATTTCAAAGAGACAAACAGCCTAACGCACAGCCCCAGTA TTTGCATGGATCCAAGGCTTTGAACCTAGCCTACTCAAGCATTTATGGCAGCTACCGGAACTTTGTGGGG CCTCCGCACTTCCAAGTCATCTGCCGGCTACTTGGCTACCAGGGCATTGCAGTAGTCATGGAAGAGCTGC TGAAGGTTGTCAAGAGCCTGCTGCAAGGCACAATCCTGCAGTACGTGAAAACTCTGATGGAAGTGATGCC CAAGATCTGCCGGCTTCCGCGACATGAGTACGGCTCTCCTGGCATCCTGGAGTTCTTCCACCACCAGCTG AAGGACATTGTGGAGTATGCAGAGCTGAAAACCGTATGCTTCCAGAACCTGCGGGAGGTGGGCAATGCTG TTCTCTTCTGCCTGCTTATTGAGCAAAGCCTGTCTTTAGAAGAAGTCTGTGACCTGTTGCATGCAGCTCC TTTCCAGAATATCTTACCTCGAATCCATGTAAAAGAGGGGGAGAGAGTTGATGCCAAAATGAAAAGACTA GAATCCAAGTATGCCCCACTGCACCTTGTCCCACTGATTGAAAGGCTGGGGACCCCACAGCAAATTGCAA TTGCAAGAGAGGGGGACTTGCTAACCAAGGAGCGTCTCTGTTGTGGTCTGTCCATGTTTGAAGTCATCCT GACACGGATCCGGACCTTTCTGGATGATCCCATCTGGCGTGGGCCCCTACCCAGCAATGGTGTCATGCAC GTGGATGAGTGTGTGGAGTTTCACAGACTATGGAGTGCCATGCAGTTTGTTTACTGCATTCCTGTAGGGA CACACGAGTTTACAGTGGAGCAGTGTTTTGGAGATGGGCTCCACTGGGCTGGCTGCATGATCATTGTACT TCTTGGACAGCAGCGGCGCTTTGCTGTGTTGGATTTCTGCTATCATCTTCTCAAAGTTCAGAAACATGAT GGCAAAGATGAGATCATCAAAAATGTGCCATTGAAGAAGATGGTGGAGAGGATCCGCAAGTTCCAGATTC TCAACGATGAAATCATCACTATCCTGGACAAGTACTTGAAATCTGGTGATGGCGAGAGCACACCTGTGGA GCATGTACGCTGCTTCCAGCCACCCATCCACCAGTCCCTAGCCAGTAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222940 representing NM_001164661
Red=Cloning site Green=Tags(s) MAAQVTLEDALSNVDLLEELPLPDQQPCIEPPPSSLLYQPNFNTNFEDRNAFVTGIARYIEQATVHSSMN EMLEEGQEYAVMLYTWRSCSRAIPQVKCNEQPNRVEIYEKTVEVLEPEVTKLMNFMYFQRNAIERFCGEV RRLCHAERRKDFVSEAYLITLGKFINMFAVLDELKNMKCSVKNDHSAYKRAAQFLRKMADPQSIQESQNL SMFLANHNKITQSLQQQLEVISGYEELLADIVNLCVDYYENRMYLTPSEKHMLLKVMGFGLYLMDGSVSN IYKLDAKKRINLSKIDKYFKQLQVVPLFGDMQIELARYIKTSAHYEENKSRWTCASSSSSPQYNICEQMI QIREDHMRFISELARYSNSEVVTGSGRQEAQKTDAEYRKLFDLALQGLQLLSQWSAHVMEVYSWKLVHPT DKYSNKDCPDNAEEYERATRYNYTTEEKFALVEVIAMIKGLQVLMGRMESVFNHAIRHTVYAALQDFSQV TLREPLRQAIKKKKNVIQSVLQAIRKTVCDWETGHEPFNDPALRGEKDPKSGFDIKVPRRAVGPSSTQLY MVRTMLESLIADKSGSKKTLRSSLEGPTILDIEKFHRESFFYTHLINFSETLQQCCDLSQLWFREFFLEL TMGRRIQFPIEMSMPWILTDHILETKEASMMEYVLYSLDLYNDSAHYALTKFNKQFLYDEIEAEVNLCFD QFVYKLADQIFAYYKVMAGSLLLDKRLRSECKNQGATIHLPPSNRYETLLKQRHVQLLGRSIDLNRLITQ RVSAAMYKSLELAIGRFESEDLTSVVELDGLLEINRMTHKLLSRYLTLDSFDAMFREANHNVSAPYGRIT LHVFWELNYDFLPNYCYNGSTNRFVRTVLPFSQEFQRDKQPNAQPQYLHGSKALNLAYSSIYGSYRNFVG PPHFQVICRLLGYQGIAVVMEELLKVVKSLLQGTILQYVKTLMEVMPKICRLPRHEYGSPGILEFFHHQL KDIVEYAELKTVCFQNLREVGNAVLFCLLIEQSLSLEEVCDLLHAAPFQNILPRIHVKEGERVDAKMKRL ESKYAPLHLVPLIERLGTPQQIAIAREGDLLTKERLCCGLSMFEVILTRIRTFLDDPIWRGPLPSNGVMH VDECVEFHRLWSAMQFVYCIPVGTHEFTVEQCFGDGLHWAGCMIIVLLGQQRRFAVLDFCYHLLKVQKHD GKDEIIKNVPLKKMVERIRKFQILNDEIITILDKYLKSGDGESTPVEHVRCFQPPIHQSLASS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001164661 |
| ORF Size | 3759 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001164661.1, NP_001158133.1 |
| RefSeq Size | 6524 bp |
| RefSeq ORF | 3762 bp |
| Locus ID | 20430 |
| UniProt ID | Q7TMB8 |
| MW | 145.7 kDa |
| Gene Summary | Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit is an adapter between EIF4E and FMR1. Promotes the translation repression activity of FMR1 in brain probably by mediating its association with EIF4E and mRNA (By similarity). Regulates formation of membrane ruffles and lamellipodia. Plays a role in axon outgrowth. Binds to F-actin but not to RNA. Part of the WAVE complex that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. Regulator of epithelial morphogenesis. May act as an invasion suppressor in cancers. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (PubMed:27605705).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG222940 | Cyfip1 (tGFP-tagged) - Mouse cytoplasmic FMR1 interacting protein 1 (Cyfip1) transcript variant 1, (10ug) |
CNY 13,780.00 |
|
| MR222940L3 | Lenti ORF clone of Cyfip1 (Myc-DDK-tagged) - Mouse cytoplasmic FMR1 interacting protein 1 (Cyfip1), transcript variant 1 |
CNY 14,440.00 |
|
| MR222940L4 | Lenti ORF clone of Cyfip1 (mGFP-tagged) - Mouse cytoplasmic FMR1 interacting protein 1 (Cyfip1), transcript variant 1 |
CNY 14,440.00 |
