Sptan1 (NM_001177668) Mouse Tagged ORF Clone
CAT#: MR222896
- TrueORF®
Sptan1 (Myc-DDK-tagged) - Mouse spectrin alpha 2 (Spna2), transcript variant 3
ORF Plasmid: tGFP
"NM_001177668" in other vectors (2)
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CNY 24,510.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2610027H02Rik; Spna-2; Spna2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR222896 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCAAGTGGGGTCAAAGTGCTGGAAACAGCCGAGGACATCCAGGAGAGACGACAGCAAGTCCTGG ATCGGTACCACCGCTTCAAGGAGCTCTCTACCTTACGGCGTCAGAAGCTGGAGGATTCCTATCGGTTCCA GTTTTTTCAAAGAGATGCTGAGGAGTTGGAGAAGTGGATTCAGGAGAAGCTTCAAGTTGCATCTGATGAG AACTACAAAGACCCAACCAACTTGCAGGGAAAGCTGCAGAAGCACCAAGCCTTTGAAGCTGAAGTACAGG CTAACTCAGGAGCCATTGTGAAGCTGGACGAGACAGGAAACTTGATGATTTCTGAAGGGCACTTTGCATC TGAAACCATCCGGACACGTTTAATGGAGCTGCACCGGCAGTGGGAACTGCTTTTGGAGAAGATGCGGGAG AAAGGAATCAAACTGCTGCAGGCACAGAAGCTGGTGCAGTATTTGCGGGAGTGTGAGGATGTAATGGACT GGATCAATGACAAGGAAGCAATTGTGACTTCTGAGGAGCTGGGCCAGGACCTGGAGCATGTAGAGGTGCT ACAGAAGAAGTTTGAAGAGTTTCAGACTGATCTGGCTGCTCATGAAGAAAGAGTTAATGAAGTGAGCCAG TTTGCTGCCAAACTCATCCAGGAGCAGCACCCGGAAGAGGAGCTGATCAAGACCAAGCAGGATGAGGTGA ATGCAGCATGGCAGCGACTGAAAGGCCTGGCTCTTCAAAGGCAGGGCAAGCTGTTCGGTGCTGCTGAGGT CCAGCGCTTTAACAGGGATGTAGATGAGACCATTGGTTGGATTAAGGAGAAAGAGCAGTTAATGGCCTCT GATGACTTCGGCAGAGACTTAGCAAGTGTTCAAGCTCTGCTTCGAAAGCATGAGGGTCTGGAGAGAGATC TTGCTGCTCTAGAGGACAAGGTGAAAGCCCTGTGTGCTGAGGCTGACCGCCTGCAACAGTCACACCCTCT GAGTGCCAGCCAGATCCAGGTGAAGCGAGAGGAGCTGATTACCAACTGGGAACAGATCCGAACTCTGGCA GCAGAGAGACATGCACGGCTTGATGACTCGTACAGGCTTCAGCGCTTTCTTGCTGACTTCCGTGACCTCA CAAGCTGGGTAACTGAAATGAAAGCCCTCATCAATGCAGATGAACTGGCCAATGATGTGGCTGGTGCTGA AGCCCTGCTGGACAGGCATCAAGAGCACAAGGGTGAGATCGACGCTCATGAAGATAGCTTTAAGTCTGCA GATGAGTCTGGGCAAGCTCTGCTTGCGGCTAGTCACTATGCCTCAGATGAAGTGAGGGAGAAGCTGAGCA TCCTCTCCGAGGAGAGAACTGCCCTGCTGGAGCTGTGGGAGCTTCGGAGGCAGCAGTATGAGCAGTGCAT GGACTTGCAGCTCTTCTACCGTGACACTGAGCAGGTGGACAACTGGATGAGCAAGCAGGAGGCATTCCTG CTAAATGAAGACTTGGGTGACTCCTTAGACAGTGTGGAAGCTCTTTTGAAGAAGCATGAGGACTTTGAAA AATCTCTCAGTGCCCAGGAAGAAAAAATCACAGCCCTTGATGAATTTGCAACCAAACTCATCCAAAATAA CCACTACGCAATGGAAGATGTAGCGACTCGACGAGATGCTCTCCTGAGCCGCCGCAATGCCCTTCATGAG CGAGCCATGCATCGCCGGGCACAGCTGGCTGATTCCTTCCATCTGCAGCAGTTCTTCCGTGATTCTGATG AGCTCAAAAGTTGGGTCAACGAGAAGATGAAAACGGCCACCGATGAAGCTTATAAAGATCCATCCAACCT GCAAGGGAAAGTCCAAAAGCATCAGGCTTTTGAGGCTGAGCTTTCAGCAAACCAGAGCCGGATTGATGCC CTTGAGAAAGCTGGGCAGAAGCTAATAGATGTCAACCACTATGCCAAGGAAGAAGTAGCAGCTCGTATGA ATGAGGTCATCAGTTTGTGGAAGAAACTTCTAGAGGCCACAGAACTGAAAGGAATAAAGCTCCGAGAAGC CAACCAGCAGCAACAATTTAATCGAAATGTTGAAGACATTGAATTGTGGCTGTATGAAGTAGAAGGTCAT TTGGCTTCAGATGATTATGGTAAAGACCTCACTAATGTCCAGAACCTCCAGAAGAAGCATGCTCTGCTAG AGGCGGATGTTGCTGCTCACCAGGATCGAATTGATGGCATCACAATTCAGGCCCGCCAGTTCCAAGATGC TGGCCATTTTGATGCCGAAAATATTAAGAAGAAGCAAGAGGCCCTTGTGGCTCGCTATGAGGCTCTTAAG GAACCCATGGTGGCCCGGAAACAGAAGCTGGCAGATTCTCTTCGTCTGCAGCAGCTCTTCCGGGATGTGG AGGACGAAGAAACCTGGATTCGAGAAAAGGAGCCTATTGCTGCATCCACTAACAGAGGCAAAGATCTTAT TGGAGTCCAGAATCTGCTAAAGAAACACCAAGCTTTGCAAGCAGAGATTGCTGGGCATGAACCTCGAATC AAAGCAGTGACACAAAAGGGAAATGCCATGGTGGAGGAAGGCCATTTTGCTGCAGAGGATGTGAAGGCCA AACTGAGTGAGCTCAACCAGAAGTGGGAGGCTCTGAAAGCCAAAGCCTCCCAGCGGAGGCAGGATCTAGA GGACTCGCTGCAGGCCCAGCAGTACTTTGCCGATGCCAATGAGGCTGAGTCCTGGATGCGGGAGAAGGAG CCCATTGTGGGCAGTACGGACTATGGGAAGGATGAAGACTCTGCTGAGGCTCTACTCAAGAAGCATGAAG CTTTGATGTCCGATCTCAGCGCCTATGGCAGCAGCATCCAGGCTCTGCGAGAACAGGCCCAGTCATGCCG GCAACAAGTGGCCCCCATGGATGATGAGACTGGGAAGGAGCTAGTCTTGGCTCTCTATGACTATCAAGAG AAGAGTCCTCGTGAGGTCACCATGAAGAAAGGGGATATCCTCACCTTGCTCAACAGCACAAACAAGGATT GGTGGAAAGTGGAAGTGAATGACCGGCAGGGTTTCGTGCCAGCTGCATATGTGAAGAAGCTGGACCCCGC CCAGTCAGCCTCGAGGGAGAACCTCCTAGAAGAGCAGGGCAGCATTGCTCTGCGGCAGGGGCAAATTGAC AACCAATATCAGTCTCTGCTGGAACTGGGTGAGAAGAGAAAAGGCATGTTGGAGAAGAGTTGCAAGAAGT TCATGTTGTTCCGGGAAGCAAACGAGTTACAGCAGTGGATCACTGAGAAGGAGGCTGCTCTAACCAATGA GGAGGTTGGCGCTGACTTGGAGCAGGTTGAGGTGCTGCAGAAGAAGTTTGATGACTTCCAGAAGGATCTG AAAGCCAATGAGTCTCGGCTGAAGGACATTAACAAAGTAGCAGAAGACCTGGAGTCTGAAGGTCTGATGG CAGAGGAAGTGCAAGCTGTGCAGCAGCAGGAGGTGTATGGTGCGATGCCCAGGGATGAAGCAGATTCCAA GACGGCCTCCCCATGGAAGTCTGCTCGACTGATGGTCCATACAGTGGCTACCTTCAACTCCATCAAGGAG CTGAATGAGCGTTGGCGGTCCCTGCAACAGCTGGCTGAGGAACGTAGCCAGCTCTTGGGCAGTGCACACG AAGTACAGAGGTTTCACAGAGATGCTGATGAAACTAAAGAATGGATTGAAGAGAAGAATCAGGCTCTGAA CACAGACAACTATGGCCATGATTTAGCTAGTGTCCAGGCCCTGCAGCGCAAACATGAAGGCTTTGAGAGG GACCTTGCAGCTCTTGGTGACAAGGTGAATTCCCTTGGGGAAACAGCCCAAAGGCTGATCCAGTCCCACC CTGAATCTGCAGAGGACTTAAAGGAAAAGTGCACAGAGTTAAACCAGGCCTGGACCAGCCTAGGGAAGCG TGCAGACCAGCGCAAGGCCAAATTGGGTGACTCCCATGATCTGCAGCGCTTCCTTAGCGATTTCCGGGAC CTCATGTCTTGGATCAATGGAATACGAGGGTTGGTATCTTCAGATGAACTAGCCAAGGACGTCACTGGAG CTGAGGCTTTGCTGGAGCGACACCAGGAACACCGGACAGAAATTGATGCCAGGGCTGGCACTTTCCAGGC ATTTGAGCAGTTTGGGCAGCAGCTGTTAGCTCACGGGCACTATGCCAGCCCGGAGATCAAAGAGAAACTT GATATTCTTGACCAGGAGCGCACAGACCTGGAGAAGGCCTGGGTCCAGCGCAGGATGATGCTGGACCACT GCCTGGAGTTGCAGCTGTTCCATCGGGACTGTGAACAAGCAGAAAACTGGATGGCTGCCCGGGAGGCCTT CCTAAACACAGAAGACAAAGGAGACTCGCTGGACAGTGTGGAGGCACTGATCAAAAAACATGAAGACTTT GACAAAGCTATCAATGTCCAGGAAGAAAAGATAGCTGCCCTGCAGGCCTTTGCCGACCAGCTCATTGCCG TTGACCACTATGCCAAGGGAGACATTGCAAACCGACGCAATGAGGTCCTTGACCGGTGGCGCCGCCTAAA AGCCCAGATGATTGAAAAAAGGTCAAAACTTGGAGAATCTCAAACACTTCAGCAGTTCAGCCGGGATGTG GATGAGATTGAAGCCTGGATCAGTGAGAAGTTACAGACAGCCAGTGATGAGTCATACAAGGACCCCACCA ACATCCAGCTTTCCAAGCTGTTGAGCAAGCACCAGAAGCACCAAGCCTTTGAGGCAGAACTGCACGCCAA TGCTGACCGAATCCGTGGAGTTATCGACATGGGCAACTCCCTCATTGAGCGTGGGGCCTGTGCTGGCAGT GAGGATGCTGTCAAGGCCCGCCTGGCTGCCCTTGCAGACCAGTGGCAGTTCCTGGTGCAGAAATCAGCTG AGAAGAGCCAGAAGCTAAAAGAGGCAAATAAGCAGCAGAACTTCAACACTGGAATCAAAGACTTTGACTT CTGGCTCTCTGAGGTGGAGGCTCTCCTGGCATCGGAAGATTATGGCAAAGACCTGGCTTCTGTGAACAAC TTGCTGAAAAAGCATCAGCTGCTAGAGGCAGACATCTCGGCCCACGAGGATCGTCTGAAGGACCTGAACA GCCAGGCTGACAGCCTGATGACTAGCAGTGCCTTCGACACCTCCCAAGTGAAAGAGAAGCGGGACACCAT CAATGGACGCTTCCAGAAGATCAAGAGCATGGCCACCTCCCGAAGAGCGAAACTGAGCGAGTCCCACCGC CTGCACCAGTTCTTCCGGGACATGGACGACGAGGAGTCCTGGATCAAGGAGAAGAAGTTGTTAGTGAGCT CTGAGGACTATGGCAGAGACCTCACTGGTGTTCAGAATCTGAGGAAGAAACACAAGCGGCTAGAAGCTGA ACTGGCTGCACATGAACCCGCCATTCAGGGTGTCCTGGACACTGGGAAGAAGCTGTCTGATGACAACACC ATCGGGCAGGAGGAGATCCAGCAACGGCTTGCACAGTTTGTGGAGCACTGGAAGGAACTAAAGCAGCTGG CGGCTGCGCGGGGCCAGCGGCTAGAGGAGTCCTTGGAGTATCAGCAGTTTGTAGCCAACGTGGAAGAGGA GGAGGCTTGGATCAATGAGAAGATGACTCTGGTGGCCAGCGAAGACTATGGAGACACTCTTGCTGCTATC CAGGGCTTACTGAAGAAACACGAAGCTTTTGAGACAGACTTCACTGTCCACAAGGATCGGGTGAATGATG TCTGTACTAATGGACAAGACCTCATTAAGAAGAACAATCACCATGAGGAGAACATCTCTTCAAAGATGAA GGGTCTGAATGGAAAGGTGTCTGATCTGGAGAAAGCAGCAGCTCAGAGGAAAGCAAAGCTGGATGAGAAC TCAGCCTTCCTTCAGTTCAACTGGAAGGCTGACGTGGTAGAGTCCTGGATTGGTGAAAAGGAGAACAGCT TGAAAACAGATGACTATGGCCGAGATCTGTCTTCTGTCCAAACTCTGCTCACAAAACAGGAGACATTTGA TGCTGGCCTGCAGGCCTTCCAGCAGGAGGGTATTGCCAATATCACTGCCCTCAAAGACCAGCTGCTAGCT GCCAAGCACATTCAGTCTAAGGCCATCGAGGCCCGACATGCCTCCCTCATGAAGAGGTGGACCCAGCTGT TGGCCAATTCAGCTACCCGGAAGAAGAAGTTGCTGGAGGCCCAGAGTCATTTCCGTAAGGTAGAAGACCT CTTCCTGACCTTTGCCAAAAAGGCATCGGCTTTCAATAGCTGGTTTGAGAATGCAGAGGAAGACCTCACA GACCCAGTGCGCTGCAACTCTCTGGAAGAAATCAAAGCCCTTCGAGAGGCTCACGATGCCTTCCGCTCAT CCCTCAGCTCTGCACAGGCTGACTTCAACCAGCTAGCCGAGCTGGACCGCCAGATCAAGAGTTTCCGGGT GGCCTCCAACCCCTATACCTGGTTCACGATGGAGGCCCTGGAAGAGACCTGGAGAAACCTCCAGAAGATC ATTAAGGAACGAGAACTGGAGCTGCAGAAGGAACAGCGGCGGCAGGAGGAGAATGACAAGCTGCGCCAAG AATTTGCCCAGCATGCCAACGCCTTCCACCAGTGGATCCAGGAAACAAGAACGTATCTCCTCGACGGGTC CTGCATGGTGGAAGAGTCGGGAACTCTGGAATCTCAGCTTGAAGCTACCAAACGCAAGCACCAGGAGATT CGAGCCATGAGAAGTCAGCTGAAGAAGATCGAGGACCTGGGGGCTGCCATGGAGGAAGCCCTCATCCTGG ACAACAAGTACACGGAGCACAGCACTGTGGGCCTGGCCCAGCAGTGGGACCAGTTAGACCAGCTGGGCAT GCGCATGCAGCACAACCTGGAGCAGCAGATCCAGGCCAGGAACACAACAGGAGTGACTGAGGAGGCCCTC AAGGAATTCAGCATGATGTTCAAACACTTCGACAAGGACAAGTCTGGCCGGCTGAACCATCAGGAGTTCA AATCCTGTCTTCGCTCCCTGGGCTATGATCTGCCAATGGTTGAGGAAGGAGAGCCTGATCCTGAGTTTGA GGCAATTCTGGACACTGTTGATCCCAACAGGGATGGCCACGTCTCCCTGCAAGAATACATGGCTTTCATG ATCAGCCGCGAAACGGAGAATGTCAAGTCCAGTGAAGAGATCGAGAGTGCTTTCCGGGCCCTCAGCTCTG AGGGCAAGCCTTATGTGACCAAGGAGGAGCTCTACCAGAACCTGACCCGGGAACAAGCTGACTATTGTGT CTCCCACATGAAGCCCTATGTGGATGGCAAGGGCCGAGAACTTCCAACTGCCTTCGACTACGTCGAGTTC ACCCGCTCACTCTTTGTGAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222896 protein sequence
Red=Cloning site Green=Tags(s) MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQVASDE NYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMRE KGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVSQ FAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETIGWIKEKEQLMAS DDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSASQIQVKREELITNWEQIRTLA AERHARLDDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSA DESGQALLAASHYASDEVREKLSILSEERTALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFL LNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHE RAMHRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDA LEKAGQKLIDVNHYAKEEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGH LASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALK EPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRI KAVTQKGNAMVEEGHFAAEDVKAKLSELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKE PIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQE KSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQGQID NQYQSLLELGEKRKGMLEKSCKKFMLFREANELQQWITEKEAALTNEEVGADLEQVEVLQKKFDDFQKDL KANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDEADSKTASPWKSARLMVHTVATFNSIKE LNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFER DLAALGDKVNSLGETAQRLIQSHPESAEDLKEKCTELNQAWTSLGKRADQRKAKLGDSHDLQRFLSDFRD LMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKL DILDQERTDLEKAWVQRRMMLDHCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDF DKAINVQEEKIAALQAFADQLIAVDHYAKGDIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDV DEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGS EDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNN LLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKEKRDTINGRFQKIKSMATSRRAKLSESHR LHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNT IGQEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAI QGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDEN SAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLA AKHIQSKAIEARHASLMKRWTQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLT DPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKI IKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEI RAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEAL KEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFM ISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEF TRSLFVN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001177668 |
ORF Size | 7371 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001177668.1, NP_001171139.1 |
RefSeq Size | 7979 bp |
RefSeq ORF | 7374 bp |
Locus ID | 20740 |
MW | 282.9 kDa |
Documents
Product Manuals |
FAQs |
SDS |