Sptan1 (NM_001177668) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610027H02Rik; Spna-2; Spna2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225322 representing NM_001177668 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCAAGTGGGGTCAAAGTGCTGGAAACAGCCGAGGACATCCAGGAGAGACGACAGCAAGTCCTGG ATCGGTACCACCGCTTCAAGGAGCTCTCTACCTTACGGCGTCAGAAGCTGGAGGATTCCTATCGGTTCCA GTTTTTTCAAAGAGATGCTGAGGAGTTGGAGAAGTGGATTCAGGAGAAGCTTCAAGTTGCATCTGATGAG AACTACAAAGACCCAACCAACTTGCAGGGAAAGCTGCAGAAGCACCAAGCCTTTGAAGCTGAAGTACAGG CTAACTCAGGAGCCATTGTGAAGCTGGACGAGACAGGAAACTTGATGATTTCTGAAGGGCACTTTGCATC TGAAACCATCCGGACACGTTTAATGGAGCTGCACCGGCAGTGGGAACTGCTTTTGGAGAAGATGCGGGAG AAAGGAATCAAACTGCTGCAGGCACAGAAGCTGGTGCAGTATTTGCGGGAGTGTGAGGATGTAATGGACT GGATCAATGACAAGGAAGCAATTGTGACTTCTGAGGAGCTGGGCCAGGACCTGGAGCATGTAGAGGTGCT ACAGAAGAAGTTTGAAGAGTTTCAGACTGATCTGGCTGCTCATGAAGAAAGAGTTAATGAAGTGAGCCAG TTTGCTGCCAAACTCATCCAGGAGCAGCACCCGGAAGAGGAGCTGATCAAGACCAAGCAGGATGAGGTGA ATGCAGCATGGCAGCGACTGAAAGGCCTGGCTCTTCAAAGGCAGGGCAAGCTGTTCGGTGCTGCTGAGGT CCAGCGCTTTAACAGGGATGTAGATGAGACCATTGGTTGGATTAAGGAGAAAGAGCAGTTAATGGCCTCT GATGACTTCGGCAGAGACTTAGCAAGTGTTCAAGCTCTGCTTCGAAAGCATGAGGGTCTGGAGAGAGATC TTGCTGCTCTAGAGGACAAGGTGAAAGCCCTGTGTGCTGAGGCTGACCGCCTGCAACAGTCACACCCTCT GAGTGCCAGCCAGATCCAGGTGAAGCGAGAGGAGCTGATTACCAACTGGGAACAGATCCGAACTCTGGCA GCAGAGAGACATGCACGGCTTGATGACTCGTACAGGCTTCAGCGCTTTCTTGCTGACTTCCGTGACCTCA CAAGCTGGGTAACTGAAATGAAAGCCCTCATCAATGCAGATGAACTGGCCAATGATGTGGCTGGTGCTGA AGCCCTGCTGGACAGGCATCAAGAGCACAAGGGTGAGATCGACGCTCATGAAGATAGCTTTAAGTCTGCA GATGAGTCTGGGCAAGCTCTGCTTGCGGCTAGTCACTATGCCTCAGATGAAGTGAGGGAGAAGCTGAGCA TCCTCTCCGAGGAGAGAACTGCCCTGCTGGAGCTGTGGGAGCTTCGGAGGCAGCAGTATGAGCAGTGCAT GGACTTGCAGCTCTTCTACCGTGACACTGAGCAGGTGGACAACTGGATGAGCAAGCAGGAGGCATTCCTG CTAAATGAAGACTTGGGTGACTCCTTAGACAGTGTGGAAGCTCTTTTGAAGAAGCATGAGGACTTTGAAA AATCTCTCAGTGCCCAGGAAGAAAAAATCACAGCCCTTGATGAATTTGCAACCAAACTCATCCAAAATAA CCACTACGCAATGGAAGATGTAGCGACTCGACGAGATGCTCTCCTGAGCCGCCGCAATGCCCTTCATGAG CGAGCCATGCATCGCCGGGCACAGCTGGCTGATTCCTTCCATCTGCAGCAGTTCTTCCGTGATTCTGATG AGCTCAAAAGTTGGGTCAACGAGAAGATGAAAACGGCCACCGATGAAGCTTATAAAGATCCATCCAACCT GCAAGGGAAAGTCCAAAAGCATCAGGCTTTTGAGGCTGAGCTTTCAGCAAACCAGAGCCGGATTGATGCC CTTGAGAAAGCTGGGCAGAAGCTAATAGATGTCAACCACTATGCCAAGGAAGAAGTAGCAGCTCGTATGA ATGAGGTCATCAGTTTGTGGAAGAAACTTCTAGAGGCCACAGAACTGAAAGGAATAAAGCTCCGAGAAGC CAACCAGCAGCAACAATTTAATCGAAATGTTGAAGACATTGAATTGTGGCTGTATGAAGTAGAAGGTCAT TTGGCTTCAGATGATTATGGTAAAGACCTCACTAATGTCCAGAACCTCCAGAAGAAGCATGCTCTGCTAG AGGCGGATGTTGCTGCTCACCAGGATCGAATTGATGGCATCACAATTCAGGCCCGCCAGTTCCAAGATGC TGGCCATTTTGATGCCGAAAATATTAAGAAGAAGCAAGAGGCCCTTGTGGCTCGCTATGAGGCTCTTAAG GAACCCATGGTGGCCCGGAAACAGAAGCTGGCAGATTCTCTTCGTCTGCAGCAGCTCTTCCGGGATGTGG AGGACGAAGAAACCTGGATTCGAGAAAAGGAGCCTATTGCTGCATCCACTAACAGAGGCAAAGATCTTAT TGGAGTCCAGAATCTGCTAAAGAAACACCAAGCTTTGCAAGCAGAGATTGCTGGGCATGAACCTCGAATC AAAGCAGTGACACAAAAGGGAAATGCCATGGTGGAGGAAGGCCATTTTGCTGCAGAGGATGTGAAGGCCA AACTGAGTGAGCTCAACCAGAAGTGGGAGGCTCTGAAAGCCAAAGCCTCCCAGCGGAGGCAGGATCTAGA GGACTCGCTGCAGGCCCAGCAGTACTTTGCCGATGCCAATGAGGCTGAGTCCTGGATGCGGGAGAAGGAG CCCATTGTGGGCAGTACGGACTATGGGAAGGATGAAGACTCTGCTGAGGCTCTACTCAAGAAGCATGAAG CTTTGATGTCCGATCTCAGCGCCTATGGCAGCAGCATCCAGGCTCTGCGAGAACAGGCCCAGTCATGCCG GCAACAAGTGGCCCCCATGGATGATGAGACTGGGAAGGAGCTAGTCTTGGCTCTCTATGACTATCAAGAG AAGAGTCCTCGTGAGGTCACCATGAAGAAAGGGGATATCCTCACCTTGCTCAACAGCACAAACAAGGATT GGTGGAAAGTGGAAGTGAATGACCGGCAGGGTTTCGTGCCAGCTGCATATGTGAAGAAGCTGGACCCCGC CCAGTCAGCCTCGAGGGAGAACCTCCTAGAAGAGCAGGGCAGCATTGCTCTGCGGCAGGGGCAAATTGAC AACCAATATCAGTCTCTGCTGGAACTGGGTGAGAAGAGAAAAGGCATGTTGGAGAAGAGTTGCAAGAAGT TCATGTTGTTCCGGGAAGCAAACGAGTTACAGCAGTGGATCACTGAGAAGGAGGCTGCTCTAACCAATGA GGAGGTTGGCGCTGACTTGGAGCAGGTTGAGGTGCTGCAGAAGAAGTTTGATGACTTCCAGAAGGATCTG AAAGCCAATGAGTCTCGGCTGAAGGACATTAACAAAGTAGCAGAAGACCTGGAGTCTGAAGGTCTGATGG CAGAGGAAGTGCAAGCTGTGCAGCAGCAGGAGGTGTATGGTGCGATGCCCAGGGATGAAGCAGATTCCAA GACGGCCTCCCCATGGAAGTCTGCTCGACTGATGGTCCATACAGTGGCTACCTTCAACTCCATCAAGGAG CTGAATGAGCGTTGGCGGTCCCTGCAACAGCTGGCTGAGGAACGTAGCCAGCTCTTGGGCAGTGCACACG AAGTACAGAGGTTTCACAGAGATGCTGATGAAACTAAAGAATGGATTGAAGAGAAGAATCAGGCTCTGAA CACAGACAACTATGGCCATGATTTAGCTAGTGTCCAGGCCCTGCAGCGCAAACATGAAGGCTTTGAGAGG GACCTTGCAGCTCTTGGTGACAAGGTGAATTCCCTTGGGGAAACAGCCCAAAGGCTGATCCAGTCCCACC CTGAATCTGCAGAGGACTTAAAGGAAAAGTGCACAGAGTTAAACCAGGCCTGGACCAGCCTAGGGAAGCG TGCAGACCAGCGCAAGGCCAAATTGGGTGACTCCCATGATCTGCAGCGCTTCCTTAGCGATTTCCGGGAC CTCATGTCTTGGATCAATGGAATACGAGGGTTGGTATCTTCAGATGAACTAGCCAAGGACGTCACTGGAG CTGAGGCTTTGCTGGAGCGACACCAGGAACACCGGACAGAAATTGATGCCAGGGCTGGCACTTTCCAGGC ATTTGAGCAGTTTGGGCAGCAGCTGTTAGCTCACGGGCACTATGCCAGCCCGGAGATCAAAGAGAAACTT GATATTCTTGACCAGGAGCGCACAGACCTGGAGAAGGCCTGGGTCCAGCGCAGGATGATGCTGGACCACT GCCTGGAGTTGCAGCTGTTCCATCGGGACTGTGAACAAGCAGAAAACTGGATGGCTGCCCGGGAGGCCTT CCTAAACACAGAAGACAAAGGAGACTCGCTGGACAGTGTGGAGGCACTGATCAAAAAACATGAAGACTTT GACAAAGCTATCAATGTCCAGGAAGAAAAGATAGCTGCCCTGCAGGCCTTTGCCGACCAGCTCATTGCCG TTGACCACTATGCCAAGGGAGACATTGCAAACCGACGCAATGAGGTCCTTGACCGGTGGCGCCGCCTAAA AGCCCAGATGATTGAAAAAAGGTCAAAACTTGGAGAATCTCAAACACTTCAGCAGTTCAGCCGGGATGTG GATGAGATTGAAGCCTGGATCAGTGAGAAGTTACAGACAGCCAGTGATGAGTCATACAAGGACCCCACCA ACATCCAGCTTTCCAAGCTGTTGAGCAAGCACCAGAAGCACCAAGCCTTTGAGGCAGAACTGCACGCCAA TGCTGACCGAATCCGTGGAGTTATCGACATGGGCAACTCCCTCATTGAGCGTGGGGCCTGTGCTGGCAGT GAGGATGCTGTCAAGGCCCGCCTGGCTGCCCTTGCAGACCAGTGGCAGTTCCTGGTGCAGAAATCAGCTG AGAAGAGCCAGAAGCTAAAAGAGGCAAATAAGCAGCAGAACTTCAACACTGGAATCAAAGACTTTGACTT CTGGCTCTCTGAGGTGGAGGCTCTCCTGGCATCGGAAGATTATGGCAAAGACCTGGCTTCTGTGAACAAC TTGCTGAAAAAGCATCAGCTGCTAGAGGCAGACATCTCGGCCCACGAGGATCGTCTGAAGGACCTGAACA GCCAGGCTGACAGCCTGATGACTAGCAGTGCCTTCGACACCTCCCAAGTGAAAGAGAAGCGGGACACCAT CAATGGACGCTTCCAGAAGATCAAGAGCATGGCCACCTCCCGAAGAGCGAAACTGAGCGAGTCCCACCGC CTGCACCAGTTCTTCCGGGACATGGACGACGAGGAGTCCTGGATCAAGGAGAAGAAGTTGTTAGTGAGCT CTGAGGACTATGGCAGAGACCTCACTGGTGTTCAGAATCTGAGGAAGAAACACAAGCGGCTAGAAGCTGA ACTGGCTGCACATGAACCCGCCATTCAGGGTGTCCTGGACACTGGGAAGAAGCTGTCTGATGACAACACC ATCGGGCAGGAGGAGATCCAGCAACGGCTTGCACAGTTTGTGGAGCACTGGAAGGAACTAAAGCAGCTGG CGGCTGCGCGGGGCCAGCGGCTAGAGGAGTCCTTGGAGTATCAGCAGTTTGTAGCCAACGTGGAAGAGGA GGAGGCTTGGATCAATGAGAAGATGACTCTGGTGGCCAGCGAAGACTATGGAGACACTCTTGCTGCTATC CAGGGCTTACTGAAGAAACACGAAGCTTTTGAGACAGACTTCACTGTCCACAAGGATCGGGTGAATGATG TCTGTACTAATGGACAAGACCTCATTAAGAAGAACAATCACCATGAGGAGAACATCTCTTCAAAGATGAA GGGTCTGAATGGAAAGGTGTCTGATCTGGAGAAAGCAGCAGCTCAGAGGAAAGCAAAGCTGGATGAGAAC TCAGCCTTCCTTCAGTTCAACTGGAAGGCTGACGTGGTAGAGTCCTGGATTGGTGAAAAGGAGAACAGCT TGAAAACAGATGACTATGGCCGAGATCTGTCTTCTGTCCAAACTCTGCTCACAAAACAGGAGACATTTGA TGCTGGCCTGCAGGCCTTCCAGCAGGAGGGTATTGCCAATATCACTGCCCTCAAAGACCAGCTGCTAGCT GCCAAGCACATTCAGTCTAAGGCCATCGAGGCCCGACATGCCTCCCTCATGAAGAGGTGGACCCAGCTGT TGGCCAATTCAGCTACCCGGAAGAAGAAGTTGCTGGAGGCCCAGAGTCATTTCCGTAAGGTAGAAGACCT CTTCCTGACCTTTGCCAAAAAGGCATCGGCTTTCAATAGCTGGTTTGAGAATGCAGAGGAAGACCTCACA GACCCAGTGCGCTGCAACTCTCTGGAAGAAATCAAAGCCCTTCGAGAGGCTCACGATGCCTTCCGCTCAT CCCTCAGCTCTGCACAGGCTGACTTCAACCAGCTAGCCGAGCTGGACCGCCAGATCAAGAGTTTCCGGGT GGCCTCCAACCCCTATACCTGGTTCACGATGGAGGCCCTGGAAGAGACCTGGAGAAACCTCCAGAAGATC ATTAAGGAACGAGAACTGGAGCTGCAGAAGGAACAGCGGCGGCAGGAGGAGAATGACAAGCTGCGCCAAG AATTTGCCCAGCATGCCAACGCCTTCCACCAGTGGATCCAGGAAACAAGAACGTATCTCCTCGACGGGTC CTGCATGGTGGAAGAGTCGGGAACTCTGGAATCTCAGCTTGAAGCTACCAAACGCAAGCACCAGGAGATT CGAGCCATGAGAAGTCAGCTGAAGAAGATCGAGGACCTGGGGGCTGCCATGGAGGAAGCCCTCATCCTGG ACAACAAGTACACGGAGCACAGCACTGTGGGCCTGGCCCAGCAGTGGGACCAGTTAGACCAGCTGGGCAT GCGCATGCAGCACAACCTGGAGCAGCAGATCCAGGCCAGGAACACAACAGGAGTGACTGAGGAGGCCCTC AAGGAATTCAGCATGATGTTCAAACACTTCGACAAGGACAAGTCTGGCCGGCTGAACCATCAGGAGTTCA AATCCTGTCTTCGCTCCCTGGGCTATGATCTGCCAATGGTTGAGGAAGGAGAGCCTGATCCTGAGTTTGA GGCAATTCTGGACACTGTTGATCCCAACAGGGATGGCCACGTCTCCCTGCAAGAATACATGGCTTTCATG ATCAGCCGCGAAACGGAGAATGTCAAGTCCAGTGAAGAGATCGAGAGTGCTTTCCGGGCCCTCAGCTCTG AGGGCAAGCCTTATGTGACCAAGGAGGAGCTCTACCAGAACCTGACCCGGGAACAAGCTGACTATTGTGT CTCCCACATGAAGCCCTATGTGGATGGCAAGGGCCGAGAACTTCCAACTGCCTTCGACTACGTCGAGTTC ACCCGCTCACTCTTTGTGAATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001177668
Insert Size	7374 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001177668.1, NP_001171139.1
RefSeq Size	7979 bp
RefSeq ORF	7374 bp
Locus ID	20740

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