Ptprf (NM_011213) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AA591035; LAR; LARS; RPTP-LAR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR222818 representing NM_011213 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCCCGAGCCAGCCCCAGGGAGGAGGATGGTGCCCCTTGTGCCTGCTCTCGTGATGCTTGGTTTGA TGGCAGGTGCTCATGGCGACAGCAAACCCGTCTTTGTTAAGGTCCCCGAGGACCAGACTGGGCTGTCGGG AGGGGTGGCCTCCTTCGTGTGCCAAGCCACAGGGGAACCCAAGCCTCGAATTACGTGGATGAAGAAGGGG AAGAAAGTCAGCTCCCAGCGCTTTGAGGTAATTGAGTTTGACGATGGAGCAGGGTCAGTGCTGCGGATCC AGCCATTACGAGTGCAGCGAGACGAAGCCATCTATGAGTGCACAGCCACGAACAGTCTCGGGGAGATCAA CACAAGTGCCAAGCTGTCAGTGCTTGAAGAGGACCAGCTGCCGTCTGGGTTCCCGACTATCGACATGGGA CCTCAGCTGAAGGTGGTGGAGAAGGGTCGCACTGCCACCATGCTGTGTGCAGCCGGTGGGAACCCAGACC CTGAGATCTCTTGGTTCAAAGACTTCCTTCCTGTGGACCCTGCTGCAAGCAACGGTCGTATCAAACAGCT GCGATCAGGTGCATTGCAGATAGAGAGCAGCGAGGAGTCTGACCAAGGCAAGTACGAGTGTGTGGCCACC AACTCTGCAGGCACACGCTACTCGGCCCCCGCCAACCTGTATGTGCGAGTGCGTCGCGTGGCTCCTCGTT TCTCCATCCCTCCCAGCAGCCAAGAGGTGATGCCGGGCGGCAGCGTGAATCTCACATGTGTGGCAGTGGG CGCGCCCATGCCGTATGTGAAATGGATGATGGGCGCCGAGGAACTGACCAAAGAGGATGAGATGCCAGTC GGCCGAAATGTTCTGGAGCTCAGCAATGTCATGCGATCTGCCAACTATACCTGTGTGGCCATCTCTTCAT TAGGCATGATAGAAGCCACGGCCCAGGTCACAGTAAAAGCTCTGCCAAAGCCTCCAATTGATCTTGTGGT GACAGAGACAACCGCCACCAGTGTTACTCTGACATGGGACTCTGGAAATACCGAGCCTGTGTCCTTCTAC GGCATCCAGTACCGCGCAGCTGGCACAGACGGCCCCTTCCAGGAAGTGGATGGTGTGGCCAGCACCCGAT ACAGCATTGGTGGCCTCAGCCCCTTTTCGGAATATGCTTTCCGTGTGTTGGCTGTTAACAGCATTGGGCG AGGACCACCCAGTGAGGCAGTGCGAGCACGCACGGGGGAGCAGGCACCCTCCAGCCCTCCGCGCCGTGTG CAGGCTCGAATGCTTAGCGCCAGCACCATGCTGGTCCAGTGGGAACCACCTGAAGAGCCCAATGGCCTGG TACGGGGATATCGCGTCTACTATACCCCAGATTCCCGACGCCCTTTGAGCGCCTGGCACAAGCATAACAC TGACGCAGGACTCCTTACCACCGTGGGCAGCCTGCTACCTGGCATCACCTACAGCCTCCGAGTCCTTGCC TTCACTGCTGTGGGTGACGGCCCACCCAGCCCCACCATCCAAGTCAAGACACAGCAGGGAGTGCCTGCAC AGCCTGCGGACTTCCAGGCTAACGCTGAGTCGGACACCAGGATCCAGCTCTCGTGGCTGCTGCCCCCGCA GGAGCGGATCGTCAAGTATGAGCTGGTGTACTGGGCAGCCGAGGATGAGGGCCAGCAGCACAAGGTGACC TTCGACCCCACCTCCTCCTACACTCTGGAGGACCTGAAGCCTGACACATTGTACCACTTCCAGCTGGCTG CCCGTTCAGATTTGGGGGTGGGCGTCTTCACGCCCACCGTAGAGGCCCGTACAGCACAGTCCACCCCATC AGCCCCTCCCCAGAAGGTGACATGTGTGAGCACGGGCTCCACCACGG[AT]CCGGGTAAGTTGGGTTCCA CCGCCGGCCGACAGCCGCAACGGCATTATCACCCAGTACTCCGTGGCCTATGAGGCAGTGGACGGCGAAG ACCGTAAGCGACATGTGGTGGATGGCATCAGCCGTGAGCATTCCAGCTGGGACCTGCTGGGCCTGGAGAA GTGGACGGAGTACCGGGTGTGGGTGCGGGCACACACAGATGTGGGCCCTGGCCCTGAGAGCAGCCCGGTG CTGGTGCGCACCGATGAGGACGTGCCTAGCGGGCCACCACGGAAGGTAGAGGTTGAGCCTCTGAACTCCA CTGCTGTGCATGTCTCCTGGAAGCTGCCCGTCCCCAACAAGCAGCACGGACAGATTCGTGGCTACCAGGT CACCTATGTGCGGTTGGAGAATGGTGAGCCCCGAGGCCAACCCATCATCCAGGATGTCATGCTGGCTGAG GCTCAGGAGACCACCATCAGCGGCCTCACCCCCGAGACCACCTACTCCATCACCGTCGCTGCCTACACCA CTAAGGGGGATGGTGCCCGAAGCAAGCCCAAGGTGGTTACTACAACAGGGGCAGTCCCAGGACGGCCCAC CATGATGGTCAGCACCACGGCCATGCACACCGCGCTGCTGCAGTGGCACCCACCCAAGGAGCTGCCTGGA GAGCTGCTGGGCTACCGTCTCCAATACCGGCGGGCCGACGAGGCGCGGCCCAACACCATAGACTTTGGCA AGGATGACCAACATTTTACAGTCACCGGCCTGCACAAAGGGGCCACCTACGTCTTCCGGCTCGCTGCCAA GAACCGGGCTGGCCCAGGAGAAGAGTTTGAGAAAGAGATCACCACCCCCGAGGATGTGCCCAGTGGCTTT CCTCAAAATCTTCGAGTGACAGGACTGACCACATCTACCACGGAACTGACCTGGGACCCGCCGGTGCTGG CGGAGAGGAATGGTCACATCACCAACTATACTGTGGTCTACCGTGACATCAACAGCCAGCTTGAACTACA GAATGTCACCAACGACACACACCTTACACTCTTGGGCCTTAAACCGGACACCACTTATGACATCAAGGTC CGTGCACATACCAGCAAAGGCGCCGGCCCTCTCAGCCCCAGCATCCAGTCCCGGACCATGCCCGTGGAGC AAGTGTTTGCCAAGAATTTCCGTGTGGCCGCTGCGATGAAGACATCTGTGCTGCTCAGTTGGGAGGTCCC CGACTCTTATAAGTCAGCTGTGCCCTTCAAGATCCTGTACAATGGGCAGAGCGTGGAGGTGGATGGGCAC TCGATGCGGAAGCTGATTGCAGACCTGCAACCCAACACGGAGTACTCCTTCGTCCTGATGAATCGTGGCA GTAGCGCCGGGGGCCTACAGCACCTGGTGTCCATCCGCACTGCCCCGGACCTCCTACCCCAGAAGCCACT GCCTGCCTCCGCCTTTATAGAGGATGGCCGCTTCTCCCTCTCCATGCCTCAAGTGCAGGACCCCTCGCTA GTCAGGTGGTTCTACATTGTGGTGGTGCCCATTGACCGTGTGGGCGGGAACTTGCTGGCACCAAGATGGA ACACACCAGAGGAGTTGGAGCTGGACGAGCTTCTGGAGGCCATCGAGCAGGGCGAGGAGAAACAGCGGAG GCGCCGGCGCCAAGCAGAGCGGCTGAAGCCTTATGTGGCGGCCCAAGTGGATGTGCTCCCTGACACCTTC ACCCTGGGGGACAAGAAGAGCTACCGCGGCTTCTACAACCGGCCCCTGTCTCCGGATCTGAGTTACCAGT GCTTCGTTCTCGCCTCCCTCAAGGAACCCATGGACCAGAAGCGCTACGCCTCCAGCCCCTACTCGGACGA GATTGTAGTCCAGGTGACGCCAGCACAGCAGCAGGAGGAGCCCGAGATGCTGTGGGTGACAGGCCCTGTC CTGGCGGTCATTCTCATCATACTCATTGTCATCGCCATCCTCCTGTTCAAGAGGAAGAGAACACACTCCC CATCATCAAAGGATGAGCAGTCAATCGGGCTGAAGGACTCCCTGTTGGCCCACTCTTCTGACCCTGTGGA GATGCGAAGGCTTAACTACCAGACCCCAGGTATGCGAGACCACCCGCCCATCCCCATCACTGACCTGGCA GACAATATTGAGCGCCTCAAAGCCAACGATGGGCTCAAGTTCTCCCAGGAGTATGAGTCCATTGACCCTG GACAGCAGTTCACATGGGAGAATTCCAACTCGGAGGTGAACAAGCCCAAGAACCGCTATGCAAATGTCAT TGCCTATGACCATTCTCGAGTCCTCCTCACCTCCATTGATGGTGTTCCTGGGAGTGACTACATCAATGCC AACTACATTGATGGCTACCGAAAGCAGAATGCCTACATCGCCACACAGGGTCCGCTGCCCGAGACCATGG GCGATTTCTGGAGGATGGTGTGGGAACAGCGCACAGCCACAGTGGTCATGATGACCAGGCTAGAGGAGAA ATCCCGGGTGAAGTGTGATCAGTATTGGCCAGTCCGTGGCACTGAGACCTATGGCCTCATTCAGGTGACC CTGGTGGACACTGTGGAGTTGGCCACATACACCATGCGCACCTTTGCCCTCCATAAGAGTGGCTCCAGTG AGAAGCGTGAGCTGCGTCAGTTCCAGTTCATGGCCTGGCCAGACCACGGCGTTCCTGAGTACCCCACTCC CATCTTGGCCTTCCTGAGACGGGTCAAGGCCTGTAACCCACTAGATGCGGGGCCCATGGTGGTGCATTGC AGTGCGGGTGTGGGGCGCACAGGCTGCTTCATCGTCATCGACGCAATGCTGGAGCGTATGAAGCACGAGA AGACGGTTGACATCTATGGCCACGTGACGTGCATGCGCTCACAAAGGAACTACATGGTGCAGACCGAGGA CCAGTATGTGTTCATCCACGAGGCCCTGCTAGAGGCTGCCATGTGCGGACACACCGAGGTGCTCGCTCGG AACCTCTATGCCCACATCCAGAAGCTAGGCCAAGTGCCTCCCGGGGAGAGCGTCACGGCCATGGAACTTG AGTTCAAGTTGCTGGCCAACTCCAAGGCCCACACGTCGCGCTTTGTCAGTGCCAACCTGCCCTGCAACAA GTTCAAGAACCGCCTAGTGAACATCATGCCCTATGAGCTGACCCGAGTGTGCTTGCAACCCATCCGTGGT GTGGAGGGCTCAGACTACATCAATGCCAGCTTTCTAGATGGCTACAGACAGCAGAAGGCCTACATAGCTA CACAGGGGCCTCTGGCAGAGAGCACCGAGGACTTCTGGCGCATGTTATGGGAGCACAATTCCACCATCAT CGTCATGCTGACCAAGCTTCGGGAGATGGGCAGGGAGAAATGTCACCAGTACTGGCCAGCAGAGCGCTCC GCTCGCTATCAGTACTTCGTTGTTGACCCGATGGCTGAGTACAACATGCCCCAGTATATTCTGCGTGAAT TCAAAGTCACAGACGCCCGGGATGGGCAGTCAAGGACAATCCGACAGTTCCAGTTTACAGACTGGCCAGA GCAAGGAGTACCCAAAACAGGTGAAGGCTTCATCGACTTCATCGGGCAGGTGCACAAGACAAAGGAGCAG TTTGGCCAGGATGGGCCCATCACGGTGCACTGCAGTGCTGGTGTGGGCCGCACCGGTGTGTTCATCACCC TGAGCATTGTCCTGGAGCGCATGCGGTATGAGGGTGTGGTTGACATGTTCCAGACCGTGAAGACCCTCCG CACACAGCGCCCTGCAATGGTGCAGACAGAGGACCAATACCAGCTGTGCTACCGTGCGGCCCTGGAATAC CTCGGCAGCTTTGATCACTATGCAACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR222818 representing NM_011213 Red=Cloning site Green=Tags(s) MAPEPAPGRRMVPLVPALVMLGLMAGAHGDSKPVFVKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKG KKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEDQLPSGFPTIDMG PQLKVVEKGRTATMLCAAGGNPDPEISWFKDFLPVDPAASNGRIKQLRSGALQIESSEESDQGKYECVAT NSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPV GRNVLELSNVMRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLVVTETTATSVTLTWDSGNTEPVSFY GIQYRAAGTDGPFQEVDGVASTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRV QARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDSRRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLA FTAVGDGPPSPTIQVKTQQGVPAQPADFQANAESDTRIQLSWLLPPQERIVKYELVYWAAEDEGQQHKVT FDPTSSYTLEDLKPDTLYHFQLAARSDLGVGVFTPTVEARTAQSTPSAPPQKVTCVSTGSTTXXRVSWVP PPADSRNGIITQYSVAYEAVDGEDRKRHVVDGISREHSSWDLLGLEKWTEYRVWVRAHTDVGPGPESSPV LVRTDEDVPSGPPRKVEVEPLNSTAVHVSWKLPVPNKQHGQIRGYQVTYVRLENGEPRGQPIIQDVMLAE AQETTISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVPGRPTMMVSTTAMHTALLQWHPPKELPG ELLGYRLQYRRADEARPNTIDFGKDDQHFTVTGLHKGATYVFRLAAKNRAGPGEEFEKEITTPEDVPSGF PQNLRVTGLTTSTTELTWDPPVLAERNGHITNYTVVYRDINSQLELQNVTNDTHLTLLGLKPDTTYDIKV RAHTSKGAGPLSPSIQSRTMPVEQVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGH SMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPQKPLPASAFIEDGRFSLSMPQVQDPSL VRWFYIVVVPIDRVGGNLLAPRWNTPEELELDELLEAIEQGEEKQRRRRRQAERLKPYVAAQVDVLPDTF TLGDKKSYRGFYNRPLSPDLSYQCFVLASLKEPMDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPV LAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGMRDHPPIPITDLA DNIERLKANDGLKFSQEYESIDPGQQFTWENSNSEVNKPKNRYANVIAYDHSRVLLTSIDGVPGSDYINA NYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPVRGTETYGLIQVT LVDTVELATYTMRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHC SAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAAMCGHTEVLAR NLYAHIQKLGQVPPGESVTAMELEFKLLANSKAHTSRFVSANLPCNKFKNRLVNIMPYELTRVCLQPIRG VEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHNSTIIVMLTKLREMGREKCHQYWPAERS ARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQ FGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEY LGSFDHYAT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_011213
ORF Size	5697 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_011213.2, NP_035343.2
RefSeq Size	7648 bp
RefSeq ORF	5697 bp
Locus ID	19268
UniProt ID	A2A8L5
MW	211.9 kDa
Gene Summary	Possible cell adhesion receptor. It possesses an intrinsic protein tyrosine phosphatase activity (PTPase) and dephosphorylates EPHA2 regulating its activity (By similarity).[UniProtKB/Swiss-Prot Function]

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