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Tnk2 (NM_016788) Mouse Tagged ORF Clone
CAT＃: MR222677
- TrueORF®
Tnk2 (Myc-DDK-tagged) - Mouse tyrosine kinase, non-receptor, 2 (Tnk2), transcript variant 1
ORF Plasmid: tGFP
"NM_016788" in other vectors (4)
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CNY 7,344.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Ack; Ack-1; Ack1; Cdgip; Pyk1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222677 representing NM_016788
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCGGAGGAGGGAACGGGCTGGCTGTTGGAGCTGCTGTCCGAGGTGCAGCTACAACAGTATTTCC TGAGGCTTCGAGATGACCTCAACATTACCCGCCTATCTCATTTTGAATATGTCAAAAATGAAGACCTGGA AAAGATTGGCATGGGCCGGCCTGGCCAGAGGCGGCTGTGGGAGGCCGTGAAGAGGAGGAAGGCCATGTGC AAACGCAAGTCATGGATGAGCAAGGTGTTCAGTGGAAAGCGGTTGGAGGCCGAGTTCCCTTCCCAGCACT CTCAGAGCACCTTCCGGAAGCCCTCCCCCACCCCAGGGAGCCTGCCAGGGGAGGGGACCCTGCAGAGCCT CACCTGCCTCATTGGGGAGAAAGACCTACGCCTGCTGGAGAAGCTGGGGGATGGCTCCTTTGGCGTGGTG CGCAGGGGTGAATGGGACGCCCCCGCAGGGAAGACGGTGAGTGTGGCCGTCAAGTGCCTGAAGCCTGACG TGCTGAGCCAGCCAGAGGCCATGGACGACTTCATCCGGGAGGTCAATGCCATGCATTCACTAGACCACCG AAACCTCATTCGCTTGTATGGTGTGGTGCTCACACTACCCATGAAGATGGTGACAGAGCTGGCACCTCTG GGATCTTTGTTGGACCGCCTACGTAAACACCAAGGTCATTTCCTCTTGGGGACGCTGAGTCGCTACGCTG TGCAGGTGGCTGAGGGTATGGCCTACCTGGAGTCCAAGCGCTTCATTCACCGGGATCTGGCTGCTCGAAA CCTGCTTTTGGCTACCCGGGACCTGGTCAAGATTGGGGACTTCGGACTGATGCGAGCTCTGCCCCAGAAT GATGACCACTATGTCATGCAAGAACACCGCAAGGTGCCCTTTGCCTGGTGTGCCCCTGAGAGCCTGAAGA CACGGACTTTCTCCCATGCCAGTGACACCTGGATGTTTGGGGTCACACTGTGGGAGATGTTCACCTATGG CCAGGAGCCCTGGATTGGCCTCAATGGCAGCCAGATCCTGCATAAGATCGACAAGGAAGGGGAGCGCCTG CCCCGGCCGGAGGACTGCCCTCAAGACATCTACAATGTCATGGTCCAGTGCTGGGCCCACAAGCCAGAGG ACAGACCCACATTTGTGGCTCTTCGGGACTTCCTGCTGGAGGCTCAGCCCACTGACATGCGGGCTCTTCA GGACTTTGAGGAGCCAGATAAACTGCACATCCAGATGAATGACGTCATCACTGTCATCGAGGGAAGGGCT GAGAACTACTGGTGGCGTGGGCAGAATACGCGGACCCTGTGTGTAGGACCCTTCCCTCGAAATGTCGTGA CCTCCGTGGCTGGCCTGTCAGCCCAGGACATCAGCCAGCCTCTACAGAATAGCTTCATTCACACAGGACA TGGTGACAGTGACCCCCGCCACTGCTGGGGGTTCCCTGACAGGATCGATGAACTGTACCTGGGAAACCCC ATGGACCCTCCTGACCTGCTGAGTGTGGAACTGAGCACCTCCCGACCCACCCAGCACCTAGGACGGGTGA AAAGGGAGCCTCCACCTCGCCCACCTCAGCCTGCCATCTTCACTCAGAAGCCAACATACGACCCTGTGAG TGAGGACCCAGACCCCCTGTCCAGCGACTTCAAGAGGCTTGGCCTGAGGAAGCCAGCCCTGCCTCGAGGG CTGTGGCTGGCAAAGCCCTCAGCCCGAGTGCCAGGCACCAAGGCAGACCGCAGCAGTGGGGGTGAGGTCA CACTCATCGACTTCGGTGAGGAGCCTGTGGTCCCAACCCCTCGGCCCTGTGCACCCTCCTTGGCACAGTT GGCCATGGATGCCTGCTCCTTGCTGGACAAGACACCACCACAGAGCCCCACACGGGCACTGCCACGACCT TTACACCCCACCCCTGTGGTAGACTGGGACGCGCGTCCGCTGCCCCCGCCCCCTGCCTATGACGACGTGG CCCAGGATGAGGATGACTTTGAGGTCTGCTCCATCAACAGCACGCTAGTAGGTGCAGGCCTCCCTGCTGG GCCTAGCCAAGGCGAGACCAATTACGCCTTTGTACCTGAGCAGGCGCAGATGCCCCCTGCCCTGGAGGAT AACCTGTTCCTTCCACCCCAGGGCGGAGGCAAGCCGCCCAGCTCAGTGCAGACTGCAGAGATTTTCCAGG CACTGCAGCAGGAGTGTATGCGGCAGCTACAGGTCCCCACTGGCCAGCTGACCCCCTCCCCGACCCCAGG AGGTGATGACAAGCCCCAGGTGCCACCCCGGGTACCTATTCCCCCTCGGCCCACGCGTCCACGTGTGGAG CTATCTCCAGCTCCCTCGGGTGAGGAAGAGACAAGCCGGTGGCCTGGACCTGCCTCGCCCCCCCGAGTGC CTCCCCGGGAACCTCTGTCTCCTCAAGGTTCAAGGACCCCAAGCCCCCTAGTGCCACCTGGCAGCTCTCC ACTACCGCATCGGCTCTCTAGCTCACCTGGAAAGACCATGCCCACCACTCAAAGCTTTGCCTCAGACCCT AAGTATGCCACTCCACAAGTGATCCAGGCTCCTGGCCCACGGGCAGGCCCCTGCATCCTGCCCATTGTCC GCGATGGCAGGAAGGTCAGCAGCACTCATTACTACCTGCTGCCTGAGCGCCCTCCTTACCTGGAACGCTA TCAGCGCTTCCTGCGGGAGGCCCAGAGCCCGGAAGAGCCGGCCGCCCTGCCTGTGCCCCCGCTGTTGCCC CCGCCCAGTACTCCAGCCCCTGCTGCCCCCACTGCCACCGTCAGACCTATGCCTCAGGCCGCCCCAGACC CAAAGGCCAACTTCTCCACCAATAACAGCAACCCAGGGGCACGGCCACCATCCCTGAGGGCCACGGCTCG GCTGCCACAGAGGGGCTGCCCAGGGGACGGGCAAGAGGCTGCTCGGCCAGCAGACAAGGTCCAGATGCTG CAGGCCATGGTGCATGGGGTGACCACAGAGGAGTGCCAGGCGGCCCTGCAGAGCCACAGCTGGAGTGTTC AGAGGGCTGCCCAGTATCTGAAGGTGGAGCAGCTCTTTGGGCTGGGTCTTCGGCCACGGGTGGAGTGCCA CAAGGTCCTAGAGATGTTCGACTGGAACCTAGAGCAAGCCGGCTGTCACCTTCTGGGCTCCTGTGGCCCT GCTCATCACAAACGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR222677 representing NM_016788
Red=Cloning site Green=Tags(s) MQPEEGTGWLLELLSEVQLQQYFLRLRDDLNITRLSHFEYVKNEDLEKIGMGRPGQRRLWEAVKRRKAMC KRKSWMSKVFSGKRLEAEFPSQHSQSTFRKPSPTPGSLPGEGTLQSLTCLIGEKDLRLLEKLGDGSFGVV RRGEWDAPAGKTVSVAVKCLKPDVLSQPEAMDDFIREVNAMHSLDHRNLIRLYGVVLTLPMKMVTELAPL GSLLDRLRKHQGHFLLGTLSRYAVQVAEGMAYLESKRFIHRDLAARNLLLATRDLVKIGDFGLMRALPQN DDHYVMQEHRKVPFAWCAPESLKTRTFSHASDTWMFGVTLWEMFTYGQEPWIGLNGSQILHKIDKEGERL PRPEDCPQDIYNVMVQCWAHKPEDRPTFVALRDFLLEAQPTDMRALQDFEEPDKLHIQMNDVITVIEGRA ENYWWRGQNTRTLCVGPFPRNVVTSVAGLSAQDISQPLQNSFIHTGHGDSDPRHCWGFPDRIDELYLGNP MDPPDLLSVELSTSRPTQHLGRVKREPPPRPPQPAIFTQKPTYDPVSEDPDPLSSDFKRLGLRKPALPRG LWLAKPSARVPGTKADRSSGGEVTLIDFGEEPVVPTPRPCAPSLAQLAMDACSLLDKTPPQSPTRALPRP LHPTPVVDWDARPLPPPPAYDDVAQDEDDFEVCSINSTLVGAGLPAGPSQGETNYAFVPEQAQMPPALED NLFLPPQGGGKPPSSVQTAEIFQALQQECMRQLQVPTGQLTPSPTPGGDDKPQVPPRVPIPPRPTRPRVE LSPAPSGEEETSRWPGPASPPRVPPREPLSPQGSRTPSPLVPPGSSPLPHRLSSSPGKTMPTTQSFASDP KYATPQVIQAPGPRAGPCILPIVRDGRKVSSTHYYLLPERPPYLERYQRFLREAQSPEEPAALPVPPLLP PPSTPAPAAPTATVRPMPQAAPDPKANFSTNNSNPGARPPSLRATARLPQRGCPGDGQEAARPADKVQML QAMVHGVTTEECQAALQSHSWSVQRAAQYLKVEQLFGLGLRPRVECHKVLEMFDWNLEQAGCHLLGSCGP AHHKR SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016788 |
| ORF Size | 3165 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016788.3, NP_058068.2 |
| RefSeq Size | 4454 bp |
| RefSeq ORF | 3168 bp |
| Locus ID | 51789 |
| UniProt ID | O54967 |
| MW | 117 kDa |
| Gene Summary | Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223395 | Tnk2 (untagged) - Mouse tyrosine kinase, non-receptor, 2 (Tnk2), transcript variant 1, (10ug) |
CNY 13,590.00 |
|
| MG222677 | Tnk2 (tGFP-tagged) - Mouse tyrosine kinase non-receptor 2 (Tnk2) transcript variant 1, (10ug) |
CNY 9,220.00 |
|
| MR222677L3 | Lenti ORF clone of Tnk2 (Myc-DDK-tagged) - Mouse tyrosine kinase, non-receptor, 2 (Tnk2), transcript variant 1 |
CNY 10,260.00 |
|
| MR222677L4 | Lenti ORF clone of Tnk2 (mGFP-tagged) - Mouse tyrosine kinase, non-receptor, 2 (Tnk2), transcript variant 1 |
CNY 10,260.00 |
