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B3gnt5 (NM_054052) Mouse Tagged ORF Clone
CAT＃: MR222661
- TrueORF®
B3gnt5 (Myc-DDK-tagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant 2
ORF Plasmid: tGFP
"NM_054052" in other vectors (5)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | beta3GnT5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222661 representing NM_054052
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGACTGTTTGTTAGCAGAAGAGTCAAAAGATGGAAAATTTTTCACTTTTTTGTCACTTGTTTTATAT TAAGCTTCATGGTTTTTTGGAGCCCAATCAATAATTACATCATGAGCCATATGAAGTCCTACTCCTACAG ATACCTCGTAAATAGCTATGGCTTTGTAAACAATTCCCTGTCTCTCAAGCACAGCTCTGTGCAGCCTCAC TACCCATACTTGATCAACCACAGAGAGAAGTGTCAGGCTCAAGATGTCCTCCTCTTACTGTTTATAAAGA CTGCCCCTGAAAACTATGGCCGACGTTCTGCAATCAGAAAAACGTGGGGCAATGAGAACTATGTTCAGTC TCAACTCAATGCCAACATCAAAATTCTGTTTGCATTAGGAACTCCTGGTCCACTGAAGGGAAAAGAACTG CAAAAAAGACTAATCGGGGAAGATCAAGTGTACAAGGATATAATTCAGCAAGATTTCATTGATTCTTTCC ACAATCTTACTTCTAAATTCCTTCTTCAGTTCAGCTGGGCAAATACCTTTTGTCCACATGCCAAATTCCT GATGACTGCTGATGATGATATATTTATCCACATGCCAAATCTCATTGAATATCTTCAAGGGCTAGAGCAG ATTGGAGTTCGAGACTTTTGGATTGGTCACGTTCATCGAGGTGGCCCTCCTGTTAGGGATAAAAGCAGCA AATACTATGTTCCCTATGAAATGTACAAGTGGCCAGCCTACCCTGACTATACAGCTGGTGCTGCCTATGT TGTCTCCAGAGATGTAGCTGCCAAAATCTATGAGGCATCGCAGACACTGAATTCCAGTATGTACATAGAT GATGTATTCATGGGCCTCTGTGCCAATAAAGTGGGAATCTTGCCACAGGACCATGTATTTTTCTCTGGGG AAGGGAAAATTCCTTATCACCCCTGCATCTATGAAAAGATGATGACATCTCACGGACACTTACAAGATCT GCAGGACCTCTGGATAGAGGCTACACATCCTAAAGTAAAGAACATTTCAAAAGGGTTTTTTGGTCAAATA TACTGCAGGTTAATTAAGATAGTTCTTCTCTGCAGACTGACTTACAGGAATTCATACCCTTGTTGGGCTG CATTTGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222661 representing NM_054052
Red=Cloning site Green=Tags(s) MRLFVSRRVKRWKIFHFFVTCFILSFMVFWSPINNYIMSHMKSYSYRYLVNSYGFVNNSLSLKHSSVQPH YPYLINHREKCQAQDVLLLLFIKTAPENYGRRSAIRKTWGNENYVQSQLNANIKILFALGTPGPLKGKEL QKRLIGEDQVYKDIIQQDFIDSFHNLTSKFLLQFSWANTFCPHAKFLMTADDDIFIHMPNLIEYLQGLEQ IGVRDFWIGHVHRGGPPVRDKSSKYYVPYEMYKWPAYPDYTAGAAYVVSRDVAAKIYEASQTLNSSMYID DVFMGLCANKVGILPQDHVFFSGEGKIPYHPCIYEKMMTSHGHLQDLQDLWIEATHPKVKNISKGFFGQI YCRLIKIVLLCRLTYRNSYPCWAAFA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_054052 |
| ORF Size | 1128 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_054052.3, NP_473393.2 |
| RefSeq Size | 4548 bp |
| RefSeq ORF | 1131 bp |
| Locus ID | 108105 |
| UniProt ID | Q8BGY6 |
| MW | 44.4 kDa |
| Gene Summary | Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X carbohydrate structures. Has strong activity toward lactosylceramide (LacCer) and neolactotetraosylceramide (nLc(4)Cer; paragloboside), resulting in the synthesis of Lc(3)Cer and neolactopentaosylceramide (nLc(5)Cer), respectively. Plays a central role in regulating neolacto-series glycolipid synthesis during embryonic development.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC212170 | B3gnt5 (untagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant 2, (10ug) |
CNY 3,990.00 |
|
| MG205836 | B3gnt5 (tGFP-tagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5) |
CNY 3,140.00 |
|
| MG222661 | B3gnt5 (tGFP-tagged) - Mouse UDP-GlcNAc:betaGal beta-13-N-acetylglucosaminyltransferase 5 (B3gnt5) transcript variant 2, (10ug) |
CNY 3,140.00 |
|
| MR222661L3 | Lenti ORF clone of B3gnt5 (Myc-DDK-tagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant 2 |
CNY 4,750.00 |
|
| MR222661L4 | Lenti ORF clone of B3gnt5 (mGFP-tagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant 2 |
CNY 4,750.00 |
