Nisch (NM_022656) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1200007D05Rik; 3202002H23Rik; AW494485; I-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR222613 representing NM_022656, codon optimized. Due to the complexity of NM_022656, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCAGCTACTCTTTCTTTCGGACCGGAACGCGAGGCGGAACCTGCTAAGGAAGCTCGAGTCGTTG GAAGCGAATTGGTGGACACCTATACTGTGTATGTGATACAGGTGACCGACGGAAACCATGAGTGGACCAT TAAGCACAGGTACTCTGATTTTCACGACCTCCATGAGAAACTGGTCGCAGAGAGGAAAATCGACAAGTCC CTGCTGCCTCCCAAGAAGATCATTGGAAAGAACTCTAGATCTCTCGTGGAGAAACGGGAAAGAGATTTGG AAGTGTATTTGCAAACTCTCCTGACCACATTTCCTGACGTAGCCCCAAGAGTCCTGGCCCACTTCCTGCA CTTCCACCTTTATGAGGTGAACGGCGTAACAGCTGCATTGGCCGAGGAGCTCTTCGAGAAGGGCGAGCAG CTCTTGGGTGCTGGAGAGGTGTTCGCAATCAGGCCACTGCAGCTTTACGCCATCACTGAACAGCTCCAGC AGGGTAAACCCACCTGCGCGTCAGGTGACGCAAAGACGGATCTCGGCCATATCTTGGATTTCACATGCAG GCTCAAGTACCTGAAGGTTTCCGGCACAGAGGGGCCTTTTGGCACGTCTAACATTAAAGAGCAGCTGCTG CCATTCGACCTGAGCATCTTCAAGTCCCTTCACCAAGTGGAGATCTCCCATTGCGACGCCAAGCACATCC GAGGACTGGTCACCAGTAAGCCAACCCTGGCTACTATGAGTGTGCGGTTTAGCGCAACATCAATGAAAGA GGTGTTGGCGCCCGAGGCCTCTGAATTTGACGAGTGGGAGCCTGAGGGCACTGCCACCCTGGGTGGACCA GTTACTGCTATTATCCCTACCTGGCAGGCTCTTACCACGCTGGACCTGTCACATAATTCAATTTGCGAAA TCGATGAAAGTGTGAAACTGATCCCGAAGATAGAATATCTTGATCTGTCACACAACGGGTTGAGAGTAGT CGACAACCTTCAGCATCTCTACAATCTTGTGCATCTGGATCTGTCTTACAACAAACTGAGCTCACTCGAG GGCGTACATACCAAATTGGGGAATGTAAAGACGCTCAATCTCGCCGGAAATTTCCTGGAAAGCCTGAGTG GACTGCATAAACTCTACAGCTTGGTCAATGTAGACCTCCGAGATAACAGAATCGAGCAGCTGGACGAAGT GAAATCTATAGGAAGCCTTCCATGTTTGGAACGACTGACCCTTCTCAATAACCCACTCTCCATCATCCCT GATTACAGAACGAAAGTGTTGTCACAGTTCGGCGAGAGAGCTAGCGAGATTTGCCTTGATGACGTAGCAA CGACTGAAAAAGAGCTCGATACAGTCGAAGTCCTGAAGGCTATTCAGAAAGCCAAGGATGTGAAGAGCAA GCTCTCTAATACCGAAAAAAAGGCGGGGGAAGACTTTAGACTGCCTCCGGCCCCCTGCATTCGGCCCGGA GGTTCCCCTCCGGCAGCACCCGCTAGCGCTTCACTGCCACAGCCTATTTTGTCTAATCAAGGCATTATGT TTGTGCAAGAGGAAGCGCTCGCCTCATCTCTCTCTTCAACCGACTCTCTGCCTCCAGAGGACCACAGGCC CATCGCCAGGGCTTGCAGTGACTCTCTCGAGAGTATTCCAGCCGGGCAGGTGGCATCAGACGACTTGCGC GATGTCCCTGGCGCAGTCGGGGGAGTTTCCCCGGACCATGCGGAACCTGAGGTTCAGGTTGTACCTGGAA GCGGCCAGATCATCTTCCTTCCATTTACTTGCATCGGGTACACCGCTACCAACCAGGACTTTATACAGCG GTTGTCCACATTGATTAGACAAGCGATTGAGCGCCAGCTGCCAGCATGGATTGAGGCTGCAAACCAGAGG GAAGAAGCGCACGGTGAACAAGGGGAAGAAGAGGAGGAGGAAGAAGAGGAAGAAGACGTGGCGGAGAATA GGTATTTTGAAATGGGCCCACCCGATGCGGAGGAAGAAGAGGGGAGTGGACAGGGCGAAGAGGACGAAGA AGACGAGGATGAGGAGGCGGAAGAGGAGCGGCTGGCCCTGGAATGGGCCCTGGGAGCTGACGAGGATTTC CTCCTGGAACATATCCGCATCTTGAAAGTGCTTTGGTGCTTTCTCATACATGTCCAGGGCAGCATCCGGC AGTTTGCTGCATGTCTGGTTCTCACCGATTTTGGGATCGCCGTGTTTGAAATTCCACACCAGGAGTCTCG GGGCAGTTCTCAACACATCCTCTCTAGTTTGAGATTTGTATTTTGTTTCCCCCATGGTGACCTGACAGAG TTCGGCTTCCTGATGCCAGAGCTCTGTCTTGTTTTGAAGGTTAGGCATAGCGAAAACACCCTTTTTATAA TCAGCGACGCCGCGAATCTCCACGAATTCCACGCTGACCTGCGCTCTTGCTTCGCACCACAACACATGGC CATGCTGTGCAGCCCTATCCTCTACGGATCCCACACTACCCTGCAAGAATTCCTCCGGCAGCTCCTCACT TTTTACAAGGTTGCCGGCGGCTCCCAAGAAAGAAGCCAGGGTTGTTTCCCCGTGTATCTCGTGTACAGCG ATAAGAGGATGGTGCAGACTCCTGCCGGAGACTATAGCGGCAACATCGAGTGGGCTAGTTGTACATTGTG TAGCGCCGTCAGGCGCTCATGTTGTGCCCCCAGTGAAGCCGTAAAGAGCGCCGCTATTCCCTACTGGCTG CTGCTCACCAGTCAACATCTCAACGTGATCAAGGCAGACTTCAATCCAATGCCTAATAGAGGGACACATA ACTGTCGGAACCGGAACTCTTTCAAGCTGAGTAGGGTGCCACTTTCTACAGTGCTGCTCGACCCTACTAG GAGCTGCACCCAGCCCAGGGGAGCCTTTGCCGACGGCCATGTACTCGAACTGCTTGTTGGGTACCGGTTC GTTACAGCCATTTTCGTCCTGCCTCATGAGAAATTCCATTTCTTGCGGGTGTACAACCAGCTGCGCGCGA GCTTGCAAGATTTGAAAACGGTGGTGATCAGCAAAAACCCGAGCGCCAAGCCGCGCAACCAACCCGCCAA GTCCCGGGCCTCTGCTGAGCAGCGCTTGCAGGAAACCCCAGCCGACGCTCCTGCGCCCGCAGCTGTGCCC CCAACCGCTAGCGCCCCTGCTCCTGCCGAAGCTCTGGCTCCCGATCTTGCACCTGTGCAGGCCCCCGGCG AAGACAGAGGACTGACCTCTGCGGAGGCACCAGCAGCAGCCGAGGCCCCCGCGGCAGCCGAAGCTCCCGC GGCCGCCGAAGCCCCAGCTGCAGCTGAAGCCCCGGCGGCCGCCGAGGCTCCAGCAGCAGCTGAGGCACCT GCACCTGCTGAAGCCCCTGCTGCAGCTGAGGCACCAGCTGCCGCAGAAGCTCCAGCGGCCGCCGAGGCTC CCGCGGCCGCCGAAGCACCAGCTTCTGCTGAAGCTCCCGCCCCCAATCAGGCTCCTGCCCCCGCAAGGGG CCCTGCGCCCGCCCGGGGCCCTGCCCCCGCGGGTGGACCTGCACCAGCCGAAGCCCTCGCTCAGGCGGAA GTGCCCGCACAGTATCCCAGCGAAAGACTGATCCAATCTACTAGCGAGGAGAACCAGATTCCATCTCATC TGCCAGTATGCCCAAGCCTCCAGCACATCGCGCGCCTCAGGGGGAGGGCAATTATCGATCTGTTTCATAA CTCAATCGCTGAGGTGGAAAACGAGGAACTTCGCCACCTGCTGTGGTCCTCAGTGGTGTTCTACCAAACC CCAGGCCTGGAGGTCACTGCTTGTGTTCTGCTGTCAAGTAAAGCAGTCTATTTTATCCTGCATGATGGCC TGCGCCGCTACTTCTCTGAGCCGCTGCAAGATTTTTGGCATCAGAAAAACACTGACTACAACAACTCACC TTTTCACGTTAGCCAGTGCTTCGTGCTGAAGCTGTCCGATTTGCAAAGTGTCAACGTGGGACTTTTCGAC CAGTACTTTAGACTGACAGGCAGCTCTCCAACACAAGTGGTGACGTGCCTTACGAGGGATAGCTATCTGA CACACTGCTTCCTGCAGCACTTGATGCTTGTCTTGTCCTCCCTCGAGCGAACACCTAGTCCAGAGCCTGT GGATAAGGATTTCTATAGCGAATTCGGGGATAAGAACACCGGAAAGATGGAAAACTATGAGCTCATCCAT AGCAGCCGCGTGAAATTTACCTATCCGTCTGAGGAGGAAGTGGGCGACCTTACCTATATCGTGGCTCAAA AGATGGCAGATCCCGCGAAGAATCCTGCACTGTCTATCCTCCTGTACATACAGGCTTTCCAGGTGGTGAC ACCACACCTGGGAAGGGGGCGCGGGCCGCTGAGGCCCAAGACTTTGCTCCTGACTTCAGCAGAAATCTTT CTGCTGGACGAAGACTATATCCACTACCCCTTGCCCGAATTCGCTAAGGAGCCCCCACAGCGGGACCGGT ACCGCTTGGACGACGGTAGGCGAGTGCGCGACCTCGACCGAGTGCTCATGGGTTACTACCCCTACCCTCA AGCACTTACCCTGGTATTCGACGATACCCAAGGCCACGACCTTATGGGCTCTGTTACTCTGGATCACTTC GGTGAGATGCCGGGTGGGCCAGGACGAGTGGGACAGGGGAGGGAAGTTCAGTGGCAAGTGTTTGTGCCAA GCGCGGAAAGTCGAGAGAAATTGATCTCTCTTCTGGCCAGACAGTGGGAGGCACTCTGCGGACGCGAACT TCCAGTCGAACTGACCGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR222613 representing NM_022656 Red=Cloning site Green=Tags(s) MAAATLSFGPEREAEPAKEARVVGSELVDTYTVYVIQVTDGNHEWTIKHRYSDFHDLHEKLVAERKIDKS LLPPKKIIGKNSRSLVEKRERDLEVYLQTLLTTFPDVAPRVLAHFLHFHLYEVNGVTAALAEELFEKGEQ LLGAGEVFAIRPLQLYAITEQLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIKEQLL PFDLSIFKSLHQVEISHCDAKHIRGLVTSKPTLATMSVRFSATSMKEVLAPEASEFDEWEPEGTATLGGP VTAIIPTWQALTTLDLSHNSICEIDESVKLIPKIEYLDLSHNGLRVVDNLQHLYNLVHLDLSYNKLSSLE GVHTKLGNVKTLNLAGNFLESLSGLHKLYSLVNVDLRDNRIEQLDEVKSIGSLPCLERLTLLNNPLSIIP DYRTKVLSQFGERASEICLDDVATTEKELDTVEVLKAIQKAKDVKSKLSNTEKKAGEDFRLPPAPCIRPG GSPPAAPASASLPQPILSNQGIMFVQEEALASSLSSTDSLPPEDHRPIARACSDSLESIPAGQVASDDLR DVPGAVGGVSPDHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEAANQR EEAHGEQGEEEEEEEEEEDVAENRYFEMGPPDAEEEEGSGQGEEDEEDEDEEAEEERLALEWALGADEDF LLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTE FGFLMPELCLVLKVRHSENTLFIISDAANLHEFHADLRSCFAPQHMAMLCSPILYGSHTTLQEFLRQLLT FYKVAGGSQERSQGCFPVYLVYSDKRMVQTPAGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWL LLTSQHLNVIKADFNPMPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVLELLVGYRF VTAIFVLPHEKFHFLRVYNQLRASLQDLKTVVISKNPSAKPRNQPAKSRASAEQRLQETPADAPAPAAVP PTASAPAPAEALAPDLAPVQAPGEDRGLTSAEAPAAAEAPAAAEAPAAAEAPAAAEAPAAAEAPAAAEAP APAEAPAAAEAPAAAEAPAAAEAPAAAEAPASAEAPAPNQAPAPARGPAPARGPAPAGGPAPAEALAQAE VPAQYPSERLIQSTSEENQIPSHLPVCPSLQHIARLRGRAIIDLFHNSIAEVENEELRHLLWSSVVFYQT PGLEVTACVLLSSKAVYFILHDGLRRYFSEPLQDFWHQKNTDYNNSPFHVSQCFVLKLSDLQSVNVGLFD QYFRLTGSSPTQVVTCLTRDSYLTHCFLQHLMLVLSSLERTPSPEPVDKDFYSEFGDKNTGKMENYELIH SSRVKFTYPSEEEVGDLTYIVAQKMADPAKNPALSILLYIQAFQVVTPHLGRGRGPLRPKTLLLTSAEIF LLDEDYIHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYYPYPQALTLVFDDTQGHDLMGSVTLDHF GEMPGGPGRVGQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPVELTG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_022656
ORF Size	4779 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_022656.2, NP_073147.2
RefSeq Size	5606 bp
RefSeq ORF	4782 bp
Locus ID	64652
UniProt ID	Q80TM9
MW	175 kDa
Gene Summary	Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension (By similarity). Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons (By similarity). Acts as a modulator of Rac-regulated signal transduction pathways. Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity. Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response element and cyclin D1 promoter activation. Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation. Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells. Inhibits lamellipodia formation, when overexpressed. Plays a role in protection against apoptosis (By similarity). Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3 (By similarity). When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures (By similarity).[UniProtKB/Swiss-Prot Function]

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