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Med1 (NM_134027) Mouse Tagged ORF Clone
CAT＃: MR222479
- TrueORF®
Med1 (Myc-DDK-tagged) - Mouse mediator complex subunit 1 (Med1), transcript variant 2
ORF Plasmid: tGFP
"NM_134027" in other vectors (4)
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CNY 4,144.00
CNY 4,940.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI480703; CRSP210; DRIP205; l11Jus15; PBP; Pparbp; TRAP220 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222479 representing NM_134027
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGCTCAGGGGGAAACCGAGGACTCAGAGAGGCTGAGTAAGATGAGCTCCCTCCTGGAACGGCTCC ATGCAAAATTTAACCAGAACAGACCTTGGAGTGAAACCATTAAGCTTGTGCGTCAAGTAATGGAGAAGAG GGTCGTAATGAGTTCTGGAGGGCATCAGCATTTGGTCAGCTGTTTGGAGACATTGCAGAAGGCTCTCAAA GTAACATCTTTGCCAGCAATGACTGATCGTTTGGAATCTATAGCCAGACAGAATGGACTGGGCTCTCACC TCAGTGCCAGTGGCACTGAGTGTTACATCACGTCAGATATGTTCTATGTGGAAGTGCAGTTAGATCCTGC AGGACAGCTTTGTGATGTCAAAGTGGCTCACCATGGGGAGAATCCTGTGAGCTGTCCAGAGCTTGTACAG CAGTTAAGGGAAAAGAATTTTGAGGAATTTTCCAAGCATCTTAAGGGTCTTGTTAATCTGTATAATCTCC CAGGGGACAACAAACTGAAGACTAAAATGTATCTGGCTCTCCAATCCTTAGAACAGGACCTTTCTAAAAT GGCTATTATGTACTGGAAGGCAACCAACGCCGCTCCCTTGGATAAGATTCTTCATGGAAGTGTTGGTTAT CTCACCCCGCGGAGTGGGGGTCATTTAATGAATATGAAATACTATGCCTCTCCATCTGACCTGCTGGATG ATAAGACTGCCTCTCCTATCATTTTGCATGAAAAGAATGTTCCTCGGTCTTTGGGAATGAATGCCTCAGT GACAATTGAAGGAACCTCTGCTATGTACAAACTCCCAATTGCCCCATTAATTATGGGGTCACACCCAGCT GACAACAAATGGACCCCTTCTTTCTCCGCAGTCACTAGTGCCAACAGTGTTGATCTTCCTGCGTGTTTCT TCTTGAAATTTCCCCAGCCAATTCCAGTATCTAAAGCATTTGTTCAGAAACTGCAAAATTGCACAGGAAT CCCGTTGTTTGAGACTCCGCCCACTTACCTGCCCCTGTATGAACTCATCACTCAGTTTGAGCTGTCAAAG GATCCTGACCCTTTACCTTTGAATCACAACATGCGATTTTACGCTGCTCTTCCAGGTCAGCAGCACTGCT ATTTTCTCAATAAAGATGCTCCTCTTCCTGATGGTCAGAGCCTGCAGGGAACACTGGTCAGCAAAATCAC CTTCCAGCACCCTGGCCGAGTTCCTCTTATCTTGAATATGATCAGACACCAAGTGGCCTATAACACTCTA ATTGGAAGCTGTGTCAAAAGAACTATTTTAAAAGAAGATTCTCCTGGGCTCCTCCAGTTTGAAGTGTGTC CTCTCTCAGAATCTCGCTTCAGTGTATCTTTTCAGCACCCTGTGAATGACTCCCTTGTGTGTGTGGTGAT GGATGTGCAAGACTCAACACATGTGAGCTGTAAACTCTACAAGGGGCTGTCAGATGCACTAATCTGTACA GACGACTTCATTGCCAAAGTTGTTCAAAGATGTATGTCCATTCCTGTGACGATGAGGGCTATTCGGAGGA AGGCTGAAACCATACAGGCTGACACCCCAGCACTGTCTCTCATTGCAGAGACAGTTGAAGACATGGTGAA AAAGAACCTGCCCCCGGCTAGCAGCCCAGGATCAAAGAATCCCGAGTTAGGATCTGGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222479 representing NM_134027
Red=Cloning site Green=Tags(s) MKAQGETEDSERLSKMSSLLERLHAKFNQNRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALK VTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQ QLREKNFEEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNAAPLDKILHGSVGY LTPRSGGHLMNMKYYASPSDLLDDKTASPIILHEKNVPRSLGMNASVTIEGTSAMYKLPIAPLIMGSHPA DNKWTPSFSAVTSANSVDLPACFFLKFPQPIPVSKAFVQKLQNCTGIPLFETPPTYLPLYELITQFELSK DPDPLPLNHNMRFYAALPGQQHCYFLNKDAPLPDGQSLQGTLVSKITFQHPGRVPLILNMIRHQVAYNTL IGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICT DDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGSKNPELGSG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_134027 |
| ORF Size | 1668 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_134027.2, NP_598788.2 |
| RefSeq Size | 2822 bp |
| RefSeq ORF | 1671 bp |
| Locus ID | 19014 |
| UniProt ID | Q925J9 |
| MW | 62 kDa |
| Gene Summary | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Essential for embryogenesis, including development of the central nervous system, heart, liver and placenta and for erythropoiesis. Also required for normal transcriptional control of thyroid-stimulating hormone beta (TSHB) in the pituitary. Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218953 | Med1 (untagged) - Mouse mediator complex subunit 1 (Med1), transcript variant 2, (10ug) |
CNY 7,130.00 |
|
| MG222479 | Med1 (tGFP-tagged) - Mouse mediator complex subunit 1 (Med1) transcript variant 2, (10ug) |
CNY 5,420.00 |
|
| MR222479L3 | Lenti ORF clone of Med1 (Myc-DDK-tagged) - Mouse mediator complex subunit 1 (Med1), transcript variant 2 |
CNY 6,840.00 |
|
| MR222479L4 | Lenti ORF clone of Med1 (mGFP-tagged) - Mouse mediator complex subunit 1 (Med1), transcript variant 2 |
CNY 6,840.00 |
