Mpig6b (NM_001191012) Mouse Tagged ORF Clone

CAT#: MR222391

  • TrueORF®

AU023871 (Myc-DDK-tagged) - Mouse expressed sequence AU023871 (AU023871), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_001191012" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,400.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms AU023871; C6orf25; G6b; Ng31
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR222391 representing NM_001191012
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTTGGTCCTGCCGCTGCTGCCTTTGTTGCTCTCAAAGGTCCAGGGGAATCCCGAGGTTTCTTTGG
AGGGCAGCCCTGGGGACCGGGTGAATCTCTCCTGCATAGGGGTCTCCGACCCCACCCGCTGGGCTTGGGC
GCCTAGTTTCCCAGCATGCAAGGGCTTGTCTAAAGGGCGCCGTCCGATCTTGTGGGCCTCGACGAGAGGG
ACCCCAACTGTGCTCCAGCATTTCTCTGGCCGCCTGCGTTCCCTGGACAATGGTATCAAGCGGCTAGAGC
TGCTGCTGAGCGCCGGGGATTCTGGAACCTTTTTCTGCAAAGGACGCCACGAGAATGAGAGTCGCACAGT
GCTTCAAGTGTTAGGGGACAAGGCAGGTTGCCGGCCTGCAGGATCTACCCACGGGTACGAGTATCCCAAG
GTCCTGATTCCGCTCCTGGGCGTTGGGCTTGTGCTGGGACTGGGAGTCGCGGGCGTGGTCTGGCGGCGGC
GCAGACTCTCGCCTCCGCCCCCACCCCCGCCCCCGCCTGGACCACTACCCACATTTGCTCCAGTCATAAA
TGCTGAGCCACAGAGGCCTTTAGAACAGGAGTCCAAGATCTCAGGCCACCTGGACCAGGAGCCGAGCCTG
CACTACGCTGATCTGGACCACTCTGTCCTCGGGAGGCACCGCCGGATGTCCACAGTGGTTTCTGGTGATG
CCTCCACTGTCTATGCGGTTGTAGTA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR222391 representing NM_001191012
Red=Cloning site Green=Tags(s)

MALVLPLLPLLLSKVQGNPEVSLEGSPGDRVNLSCIGVSDPTRWAWAPSFPACKGLSKGRRPILWASTRG
TPTVLQHFSGRLRSLDNGIKRLELLLSAGDSGTFFCKGRHENESRTVLQVLGDKAGCRPAGSTHGYEYPK
VLIPLLGVGLVLGLGVAGVVWRRRRLSPPPPPPPPPGPLPTFAPVINAEPQRPLEQESKISGHLDQEPSL
HYADLDHSVLGRHRRMSTVVSGDASTVYAVVV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001191012
ORF Size 726 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001191012.1, NP_001177941.1
RefSeq Size 2382 bp
RefSeq ORF 729 bp
Locus ID 106722
UniProt ID D7PDD4
MW 26.5 kDa
Gene Summary Inhibitory receptor that acts as a critical regulator of hematopoietic lineage differentiation, megakaryocyte function and platelet production (PubMed:23112346). Inhibits platelet aggregation and activation by agonists such as ADP and collagen-related peptide (By similarity). This regulation of megakaryocate function as well as platelet production ann activation is done through the inhibition (via the 2 ITIM motifs) of the receptors CLEC1B and GP6:FcRgamma signaling (PubMed:23112346). Appears to operate in a calcium-independent manner (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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