United States
Germany
Japan
United Kingdom
China
Derl3 (NM_024440) Mouse Tagged ORF Clone
CAT＃: MR222306
- TrueORF®
Derl3 (Myc-DDK-tagged) - Mouse Der1-like domain family, member 3 (Derl3)
ORF Plasmid: tGFP
"NM_024440" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1810006I20Rik; 1810063P04Rik; derlin-3; IZP6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222306 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGGCAGAGGCTGGCGGCGGGCTTCCTGCAGGTGCCCGCGGTGACACGCGCCTACACCGCGGCCT GCGTCCTCACCACGGCCGCAGTGCAGCTGGAGCTCCTCAGCCCCTTCCAGCTCTACTTCAACCCGCACCT GGTGTTCCGGAAGTTCCAGGTCTGGAGGCTCATCACCACCTTCCTCTTCTTCGGGCCCCTGGGATTCGGC TTCTTTTTCAACATGCTCTTCGTGTTCCGCTACTGCCGCATGCTGGAGGAGGGTTCTTTCCGTGGCCGCA AGGCTGACTTCGTTTTCATGTTTCTCTTCGGTGGTGTTCTTATGACTCTGCTGGGATTCCTGGGCAGCCT GTTTTTCCTGGGACAGGCCCTCATGGCCATGCTGGTCTATGTATGGAGCCGTCGCAGCCCTCACGTGAGG GTCAACTTCTTCGGCTTACTCAACTTCCAGGCACCATTCCTGCCCTGGGCCCTCATGGGCTTCTCGCTGC TGCTGGGCAACTCGGTTGTCACAGACCTGTTAGGGATCCTTGTGGGCCACATCTACTACTTCCTAGAAGA CGTCTTTCCCAACCAGCCCGGAGGCAAGAGACTGCTGCTGACCCCCAGCGTGTTGAAGCTGCTACTAGAT GACCCTCAGGAAGACCCCGATTACCTGCCCCTTCCCGAAGAGCAGCCAGAGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222306 protein sequence
Red=Cloning site Green=Tags(s) MAGQRLAAGFLQVPAVTRAYTAACVLTTAAVQLELLSPFQLYFNPHLVFRKFQVWRLITTFLFFGPLGFG FFFNMLFVFRYCRMLEEGSFRGRKADFVFMFLFGGVLMTLLGFLGSLFFLGQALMAMLVYVWSRRSPHVR VNFFGLLNFQAPFLPWALMGFSLLLGNSVVTDLLGILVGHIYYFLEDVFPNQPGGKRLLLTPSVLKLLLD DPQEDPDYLPLPEEQPEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024440 |
| ORF Size | 687 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024440.2, NP_077760.1 |
| RefSeq Size | 1336 bp |
| RefSeq ORF | 687 bp |
| Locus ID | 70377 |
| UniProt ID | Q9D8K3 |
| MW | 26 kDa |
| Gene Summary | Functional component of endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal glycoproteins, but not that of misfolded nonglycoproteins. May act by forming a channel that allows the retrotranslocation of misfolded glycoproteins into the cytosol where they are ubiquitinated and degraded by the proteasome. May mediate the interaction between VCP and the misfolded glycoproteins. May be involved in endoplasmic reticulum stress-induced pre-emptive quality control, a mechanism that selectively attenuates the translocation of newly synthesized proteins into the endoplasmic reticulum and reroutes them to the cytosol for proteasomal degradation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC211061 | Derl3 (untagged) - Mouse Der1-like domain family, member 3 (Derl3), (10ug) |
CNY 3,990.00 |
|
| MG222306 | Derl3 (tGFP-tagged) - Mouse Der1-like domain family member 3 (Derl3), (10ug) |
CNY 2,850.00 |
|
| MR222306L3 | Lenti ORF clone of Derl3 (Myc-DDK-tagged) - Mouse Der1-like domain family, member 3 (Derl3) |
CNY 4,750.00 |
|
| MR222306L4 | Lenti ORF clone of Derl3 (mGFP-tagged) - Mouse Der1-like domain family, member 3 (Derl3) |
CNY 4,750.00 |
