Piga (NM_011081) Mouse Tagged ORF Clone
CAT#: MR222212
- TrueORF®
Piga (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class A (Piga)
ORF Plasmid: tGFP
"NM_011081" in other vectors (4)
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CNY 3,656.00
Cited in 4 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AI194334; Pig-a |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR222212 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAATAGAAGAGGAGGTGGGCAAGGCCAGCCTCCTTCAGTGTCGCCTTCCCCGGGTAGCTCTGGAA ACCTTTCCGACGATAGGACCTGCACCCATAATATATGCATGGTGTCAGACTTTTTCTACCCAAACATGGG AGGCGTGGAAAGCCACATTTACCAGCTCTCTCAGTGCCTCATTGAGAGAGGGCACAAGGTCATAACTGTC ACCCATGCTTATGGAAATCGAAAGGGCGTCCGTTACCTCACCAATGGCCTCAAAGTTTACTATTTGCCTC TGAGAGTCATGTACAACCAATCTACAGCCACGACTCTCTTTCACAGTCTGCCATTGCTCAGGTACATATT TGTTCGGGAGAGAATTACGATAATCCATTCCCACAGTTCTTTCTCTGCCATGGCCCATGATGCTCTCTTC CACGCCAAGACAATGGGGCTTCAGACAGTCTTCACAGACCATTCCCTTTTCGGATTTGCTGATGTCAGCT CGGTGCTTACAAACAAGCTTCTAACTGTGTCTCTTTGTGACACAAACCACATCATTTGTGTCTCTTACAC TAGTAAGGAGAACACTGTACTCAGAGCAGCACTGAATCCTGAAATAGTGTCCGTCATTCCTAACGCTGTA GATCCTACTGACTTCACTCCAGACCCATTTAGGAGGCATGATAGTGTAATAACTGTTGTTGTTGTCAGCA GACTTGTTTACAGAAAAGGGACTGATTTGCTTAGTGGTATAATACCTGAACTCTGTCAGAAATACCAAGA ATTACATTTCCTAATTGGAGGAGAGGGACCAAAGAGAATCATTTTGGAAGAAGTACGGGAAAGATACCAA CTACATGACAGGGTGCAGCTTTTGGGAGCTTTAGAACACAAGGATGTTAGAAATGTCTTAGTTCAAGGAC ACATTTTTCTTAACACCTCCCTCACTGAAGCGTTCTGCATGGCCATCGTGGAAGCTGCCAGTTGTGGTTT GCAGGTCGTCAGCACAAAGGTTGGTGGGATTCCCGAAGTCCTTCCGGAAAGTCTTATTATTCTCTGCGAG CCTTCAGTAAAATCTTTGTGTGACGGTCTGGAAAAAGCCATTTTCCAAGTGAAGTCGGGGACATTGCCAG CTCCAGAAAATATCCACAATGTAGTGAAGACTTTCTACACCTGGCGGAACGTGGCAGAGAGAACTGAAAA GGTGTATGAGCGAGTGTCGAAGGAAACTGTTTTGCCAATGCACAAGAGATTGGACAGGCTCATCTCACAC TGTGGCCCTGTGACGGGCTACATGTTTGCTTTGCTGGCTGTGCTCAGCTATCTCTTCCTCATTTTCCTGC AGTGGATGACTCCAGACTCTTTCATTGATGTTGCAATAGATGCTACTGGGCCAAGGAGAGCCTGGACTCA TCAATGGCCTCGTGATAAGAAAAGAGATGAGAATGATAAGATATCCCAAAGCAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222212 protein sequence
Red=Cloning site Green=Tags(s) MANRRGGGQGQPPSVSPSPGSSGNLSDDRTCTHNICMVSDFFYPNMGGVESHIYQLSQCLIERGHKVITV THAYGNRKGVRYLTNGLKVYYLPLRVMYNQSTATTLFHSLPLLRYIFVRERITIIHSHSSFSAMAHDALF HAKTMGLQTVFTDHSLFGFADVSSVLTNKLLTVSLCDTNHIICVSYTSKENTVLRAALNPEIVSVIPNAV DPTDFTPDPFRRHDSVITVVVVSRLVYRKGTDLLSGIIPELCQKYQELHFLIGGEGPKRIILEEVRERYQ LHDRVQLLGALEHKDVRNVLVQGHIFLNTSLTEAFCMAIVEAASCGLQVVSTKVGGIPEVLPESLIILCE PSVKSLCDGLEKAIFQVKSGTLPAPENIHNVVKTFYTWRNVAERTEKVYERVSKETVLPMHKRLDRLISH CGPVTGYMFALLAVLSYLFLIFLQWMTPDSFIDVAIDATGPRRAWTHQWPRDKKRDENDKISQSR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_011081 |
ORF Size | 1458 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011081.3 |
RefSeq Size | 3593 bp |
RefSeq ORF | 1458 bp |
Locus ID | 18700 |
UniProt ID | Q64323 |
MW | 54.5 kDa |
Gene Summary | Necessary for the synthesis of N-acetylglucosaminyl-phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis.[UniProtKB/Swiss-Prot Function] |
Citations (4)
The use of this cDNA Clones has been cited in the following citations: |
---|
CNS glycosylphosphatidylinositol deficiency results in delayed white matter development, ataxia and premature death in a novel mouse model
,null,
Human Molecular Genetics
,PubMed ID 32179897
[Piga]
|
CNS Glycosylphosphatidylinositol Deficiency Results in Delayed White Matter Development, Ataxia, and Premature Death in a Novel Mouse Model
,Lukacs, M;Stottmann, RW;,
bioRxiv
[PIGA]
|
Glycosylphosphatidylinositol biosynthesis and remodeling are required for neural tube closure, heart development, and cranial neural crest cell survival
,Lukacs, M;Roberts, T;Chatuverdi, P;Stottmann, RW;,
Elife
,PubMed ID 31232685
[PIGA]
|
Glycosylphosphatidylinositol Biosynthesis and Remodeling are Required for Neural Crest Cell, Cardiac and Neural Development
,Lukacs, M;Roberts, T;Chaturvedi, P;Stottmann, R;,
bioRxiv
[PIGA]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC216641 | Piga (untagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class A (Piga), (10ug) |
CNY 6,270.00 |
|
MG222212 | Piga (tGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis class A (Piga), (10ug) |
CNY 5,256.00 |
|
MR222212L3 | Lenti ORF clone of Piga (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class A (Piga) |
CNY 5,230.00 |
|
MR222212L4 | Lenti ORF clone of Piga (mGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class A (Piga) |
CNY 5,230.00 |