Ptprj (NM_008982) Mouse Tagged ORF Clone
CAT#: MR221777
- TrueORF®
Ptprj (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, receptor type, J (Ptprj), transcript variant 1
ORF Plasmid: tGFP
"NM_008982" in other vectors (4)
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CNY 9,240.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AI450271; BET; Byp; CD148; DEP-1; Ptpb2; PTPbeta2; RPTPJ; Scc-1; Scc1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR221777 representing NM_008982
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGCGCGGAGGAGGCAGAGGGAGCAGCCGGGGCCGCGGGAGCCGGGAGCTGGGAGCCACGCGCGGAG GGTGGGCGCCGCTCGCTCCGCCCCGCGAAGCCCCTGCGAGTCTAAGGCCGCGGCCGCTCCGCGCCAGGCG CGCTAGGCTCCGCCGTGTGGCCGCCGCCGCCGCCGCTGCCATGTCCCCGGGGAAGCCCGGGGCGGGCGGA GCGGGGACTAGGCGGACCGGCTGGCGGAGAAGGAGGCGGAGGCGTCGGCTGGAGACGGAGACGAGGGCGC CCGGCTTCGGGCACACGGCGGGGCGCGTCCCGGGCACGTTCCAGGGCGCGCAGGGCATGAAGCCCGCGGC GCGGGAGACGCGGACACCCCCGCGCTCGCCCGGGCTCCGCTGGGCGCTGCTGCCGCTGCTGCTGTTGCTA CGCCAGGGCCAGGTCCTGTGCGCAGGTGCGGCACCGAATCCTATATTTGACATTGAAGCTGTCGTCAGCC CAACTAGTGTGTTATTAACATGGAAGCACAATGACTCAGGCGCTTCAGAATGTAGAATAGAGAATAAGAT GGAGAGCAATCTGACGTTTCCTGTTAAAAACCAGACATCATGTAACATTACAGGCTTAAGCCCAGGCACT TCGTATACATTCTCCATCATCTCTGTAACAACCAATGAGACCTTGAACAAAACTATCACAACAGAGCCCT GGCCAGTGTCTGATCTCCATGTCACCTCTGTGGGTGTGACACAGGCTCGTCTCACCTGGAGCAATGCAAA TGGCACTGCCTCCTACCGGATGCTGATTGAAGAGTTGACCACACATTCCTCAGTCAATATTTCAGGTCTG AAGCCGGGGACCAATAATAGCTTCGCTTTCCCAGAATCAAATGAGACACAGGCTGACTTTGCAGTTGCAG AGGAGGTCCCGGATGCCAATGGTACCAAGAGAATCCCAGTGACCAACCTATCCCAACTACACAAGAATTC TCTTGTCTCTGTGGACCCACCCTCTGGCCAGGATCCCTCCCTCACAGAGATCTTGCTTACTGACCTAAAG CCTGATACTCAGTACAATGCCACCATCTATTCTCAAGCAGCAAATGGCACTGAAGGACAGCCCAGGAACA AAGTGTTTAAAACAAATTCCACCCAGGTTTCTGACGTCCGAGCTATGAACATCAGTGCCTCAAGCATGAC CCTGACCTGGAAAAGCAATTACGATGGGTCCCGTACTTCAATTGTCTACAAAATACACGTGGCTGGGGGG ACCCACTCCGTCAACCAAACTGTCAATAAGACTGAGGCCATCATCCTCGGACTCAGCTCCAGCACCTTGT ACAACATCACAGTTCATCCTTTCCTGGGTCAGACGGAGGGCACACCAGGCTTCCTCCAAGTGTACACTTC CCCCGATCAGGTCTCTGACTTCCGAGTGACAAATGTCAGCACAAGGGCAATTGGTTTGGCTTGGAGGAGC AATGACTCCAAGTCCTTCGAGATTTTCATCAAGCAGGACGGAGGTGAGAAGCATCGAAATGCTTCGACGG GAAACCAGAGCTATATGGTTGAAGATTTAAAGCCTGGAACCAGTTACCATTTTGAGATAATTCCACGAGG ACCAGACGGGACAGAAGGGCTGTCCAGTACAGTGAATGGGAGCACTGACCCCAGTGCTGTGACTGACATC CGGGTGGTCAACATTAGCACCACTGAAATGCAGTTGGAGTGGCAGAATACGGACGATGCCTCTGGATACA CTTACCATTTAGTTCTAGAGTCTAAAAGTGGCTCCATCATCAGGACCAACAGTTCTCAGAAGTGGATCAC AGTAGGGAGCCTCACCCCAGGCACCTTATACAATGTCACAATCTTTCCAGAAGTGGACCAGATCCAGGGA ATCTCCAACTCCATTACCCAGTACACACGGCCCAGCAGTGTGTCCCACATTGAAGTAAACACCACCACCA CCACGGCAGCCATCCGATGGAAGAACGAGGACGCAGCCTCTGCTTCCTATGCCTACTCCGTCCTTATCTT GAAGACTGGAGATGGCAGCAATGTAACCAGCAACTTCACAAAAGACCCTTCTATTCTAATCCCTGAGTTA ATCCCTGGTGTCTCTTACACAGTGAAGATCCTTACACAAGTTGGGGATGGTACAACATCACTGGTACCTG GTTGGAATCTGTTCTGTACGGAACCTGAACCAGTGACCTCCTTCCACTGTGAAGTGGTCCCTAAGGAGCC AGCATTGGTTCTCAAGTGGGCCTGCCCCTTTGGCATGTACACAGGCTTCGAGCTGGGGGTCAGGAGTGAT TCCTGGGACAATATGACACGCCTAGAGAACTGCACTTCGGATGATGACACAGAGTGCAGGACGGAAGTCG CCTATTTGAATTTTTCTACCTCGTACAACATCAGCATCGCCACCTTGTCATGTGGGAAGATGGCGCTTCC CGCCCAGAACATCTGCACCACTGGCATCACAGACCCACCTACTCCGGATGGATCCCCTAATATTACATCG GTCAGTCACAATTCAGTAAAGGTTAAGTTCAGCGGGTTTGAAGCCAGCCACGGACCTATCAAAGCCTATG CTGTCATCCTCACCACCGGGGAAGCTGCCCAACCTTCTGCAGATGTTTTGAAGTACACGTATGAGGATTT CAAAAGGGGAGCCTCGGATACTTATGTCACATACCTCATAAGAATAGAAGAGAAGGGACAGTCTCAGGGC TTGTCTGAAGTCTTGAACTATGAAATTGATGTGGGGAACCAATCCACTACCCTCGGCTACTACAACGGGA GGCTGGAGCCTCTGGGCTCCTACCGGGCTTGTGTTGCTGGCTTTACCAATATTACCTACAACCTTCAGAA TGACGGCCTCATCAATGGGGATGAGAGCTATGTGTCTTTCAGTCCATATTCAGAGGCCGTGTTCTTGCCC CAGGACCCAGGTGTCATCTGCGGAGCAGTGTTTGGATGTATCTTTGGTGCCCTGGCCATCACAGCTGTGG GAGGCTTCATCTTCTGGAGAAAGAAAAGGACAGATGCCAAGAATAATGAAGTGTCCTTTTCTCAAATTAA ACCTAAAAAATCCAAGTTAATCCGAGTGGAGAATTTTGAGGCCTACTTTAAGAAGCAGCAAGCTGACTCT AACTGTGGGTTTGCAGAGGAATATGAGGACCTGAAGCTGATTGGGATAAGTTTACCTAAATACACAGCTG AGATAGCCGAGAACAGAGGGAAGAACCGCTACAACAATGTTCTGCCCTATGATATTTCTCGAGTCAAACT TTCAGTCCAGACCCATTCGACAGATGACTACATCAATGCCAACTATATGCCTGGCTACCATTCCAAGAAA GATTTCATTGCCACACAAGGACCTTTACCCAACACTTTGAAAGATTTCTGGCGTATGGTTTGGGAGAAAA ACGTATATGCCATTGTTATGTTGACCAAATGCGTGGAGCAGGGAAGGACCAAATGTGAGGAGTACTGGCC TTCCAAGCAGGCTCAGGACTACGGGGACATAACTGTGGCGATGACATCAGAAGTCGTTCTTCCAGAATGG ACCATCAGAGATTTTGTGGTGAAAAATATGCAGAATAGCGAGAGCCATCCTCTGCGGCAGTTCCATTTCA CCTCCTGGCCTGACCACGGTGTTCCTGACACCACTGACCTGCTCATCAACTTTCGGTACCTGGTCCGGGA TTACATGAAGCAGATACCCCCCGAGTCACCAATTCTGGTGCATTGCAGTGCTGGGGTTGGAAGGACGGGC ACTTTCATCGCCATCGATCGCCTGATCTATCAGATAGAGAATGAGAACACCGTGGACGTGTATGGGATTG TCTATGACCTTCGGATGCACAGGCCTCTGATGGTGCAGACAGAGGACCAGTATGTTTTCCTCAATCAGTG TGTTTTGGATATTATCAGAGCCCAGAAAGACTCAAAAGTTGATCTCATCTATCAGAACACAACGGCAATG ACAATCTATGAAAACCTCGAGCCAGTGAGCATGTTTGGAAAGACTAATGGTTACATCGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR221777 representing NM_008982
Red=Cloning site Green=Tags(s) MTRGGGRGSSRGRGSRELGATRGGWAPLAPPREAPASLRPRPLRARRARLRRVAAAAAAAMSPGKPGAGG AGTRRTGWRRRRRRRRLETETRAPGFGHTAGRVPGTFQGAQGMKPAARETRTPPRSPGLRWALLPLLLLL RQGQVLCAGAAPNPIFDIEAVVSPTSVLLTWKHNDSGASECRIENKMESNLTFPVKNQTSCNITGLSPGT SYTFSIISVTTNETLNKTITTEPWPVSDLHVTSVGVTQARLTWSNANGTASYRMLIEELTTHSSVNISGL KPGTNNSFAFPESNETQADFAVAEEVPDANGTKRIPVTNLSQLHKNSLVSVDPPSGQDPSLTEILLTDLK PDTQYNATIYSQAANGTEGQPRNKVFKTNSTQVSDVRAMNISASSMTLTWKSNYDGSRTSIVYKIHVAGG THSVNQTVNKTEAIILGLSSSTLYNITVHPFLGQTEGTPGFLQVYTSPDQVSDFRVTNVSTRAIGLAWRS NDSKSFEIFIKQDGGEKHRNASTGNQSYMVEDLKPGTSYHFEIIPRGPDGTEGLSSTVNGSTDPSAVTDI RVVNISTTEMQLEWQNTDDASGYTYHLVLESKSGSIIRTNSSQKWITVGSLTPGTLYNVTIFPEVDQIQG ISNSITQYTRPSSVSHIEVNTTTTTAAIRWKNEDAASASYAYSVLILKTGDGSNVTSNFTKDPSILIPEL IPGVSYTVKILTQVGDGTTSLVPGWNLFCTEPEPVTSFHCEVVPKEPALVLKWACPFGMYTGFELGVRSD SWDNMTRLENCTSDDDTECRTEVAYLNFSTSYNISIATLSCGKMALPAQNICTTGITDPPTPDGSPNITS VSHNSVKVKFSGFEASHGPIKAYAVILTTGEAAQPSADVLKYTYEDFKRGASDTYVTYLIRIEEKGQSQG LSEVLNYEIDVGNQSTTLGYYNGRLEPLGSYRACVAGFTNITYNLQNDGLINGDESYVSFSPYSEAVFLP QDPGVICGAVFGCIFGALAITAVGGFIFWRKKRTDAKNNEVSFSQIKPKKSKLIRVENFEAYFKKQQADS NCGFAEEYEDLKLIGISLPKYTAEIAENRGKNRYNNVLPYDISRVKLSVQTHSTDDYINANYMPGYHSKK DFIATQGPLPNTLKDFWRMVWEKNVYAIVMLTKCVEQGRTKCEEYWPSKQAQDYGDITVAMTSEVVLPEW TIRDFVVKNMQNSESHPLRQFHFTSWPDHGVPDTTDLLINFRYLVRDYMKQIPPESPILVHCSAGVGRTG TFIAIDRLIYQIENENTVDVYGIVYDLRMHRPLMVQTEDQYVFLNQCVLDIIRAQKDSKVDLIYQNTTAM TIYENLEPVSMFGKTNGYIA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
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Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_008982 |
ORF Size | 4050 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008982.5, NP_033008.3 |
RefSeq Size | 7634 bp |
RefSeq ORF | 4053 bp |
Locus ID | 19271 |
UniProt ID | Q64455 |
MW | 149 kDa |
Gene Summary | Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, RET, KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Involved in vascular development. May be involved in the mechanism of contact inhibition of cell growth. Regulator of macrophage adhesion and spreading. Positively affects cell-matrix adhesion. Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration; through dephosphorylation of PDGFR. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation; through KDR dephosphorylation. Negative regulator of EGFR signaling pathway; through EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC224301 | Ptprj (untagged) - Mouse protein tyrosine phosphatase, receptor type, J (Ptprj), transcript variant 1, (10ug) |
CNY 17,290.00 |
|
MG221777 | Ptprj (tGFP-tagged) - Mouse protein tyrosine phosphatase receptor type J (Ptprj) transcript variant 1, (10ug) |
CNY 12,920.00 |
|
MR221777L3 | Lenti ORF clone of Ptprj (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, receptor type, J (Ptprj), transcript variant 1 |
CNY 11,640.00 |
|
MR221777L4 | Lenti ORF clone of Ptprj (mGFP-tagged) - Mouse protein tyrosine phosphatase, receptor type, J (Ptprj), transcript variant 1 |
CNY 13,680.00 |