Kif13b (NM_001081177) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	5330429L19Rik; 6030414C01; AI429803; C130021D12Rik; GAKIN
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR221195 representing NM_001081177 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGACTCCAAAGTGAAAGTGGCGGTACGGGTCCGGCCAATGAACCGACGAGAAATTGACTTACATA CCAAATGTGTGGTGGATGTTGAAGCGAACAAAGTTATTCTGAATCCTGTAAATACAAATCTTTCCAAAGG AGATGCCCGGGGCCAGCCAAAGATCTTTGCTTATGATCACTGTTTCTGGTCCATGGATGAATCTGTCCGA GAGAAGTATGCAGGTCAAGAAGATGTTTTCAAGTGCCTTGGGGAGAACATTCTTCAGAATGCATTTGATG GCTACAACGCCTGTATCTTTGCTTATGGGCAGACAGGGTCTGGAAAGTCTTACACCATGATGGGCACTGC TGACCAACCTGGATTAATCCCAAGGCTCTGTAGTGGACTCTTTGAACGAACCCAGAAAGAGGAAAATGAA GAACAGAGTTTTAAAGTCGAAGTGTCGTACATGGAAATTTATAATGAAAAAGTCCGAGACCTTCTTGACC CCAAAGGAAGCCGTCAGACGTTGAAAGTGAGAGAGCACAGTGTGTTGGGACCCTACGTGGATGGACTTTC TAAACTGGCTGTCACAAGCTACAAGGATATTGAGTCTCTGATGTCTGAGGGCAACAAGTCTCGGACAGTT GCTGCAACCAACATGAACGAGGAAAGTAGCCGTTCCCATGCAGTCTTCAAAATCACCCTGACACATACTC TGTATGATGTGAAGTCTGGGACATCTGGAGAGAAAGTGGGCAAGCTGAGCTTAGTGGATTTAGCTGGCAG TGAACGAGCCACGAAAACCGGAGCTGCGGGCGACAGGCTGAAGGAGGGCAGCAACATCAACAAGTCCCTC ACAACCCTGGGTCTGGTTATCTCTGCCCTAGCTGATCAGGGTGCTGGCAAAAACAAAAATAAATTTGTTC CCTATCGGGACTCAGTTCTCACATGGCTGCTCAAGGACAGTCTGGGAGGTAATAGCAAGACTGCCATGGT GGCCACTGTGAGCCCCGCTGCAGATAACTATGACGAAACCCTCTCAACCCTGAGGTACGCAGATCGAGCA AAGCACATCGTCAACCATGCTGTGGTGAATGAGGATCCGAATGCTCGGATTATCCGAGATCTGCGGGAGG AGGTGGAGAAGCTCCGGGAGCAGCTGACCAAGGCAGAGGCAATGAAATCTCCAGAGCTGAAAGATCGTCT GGAAGAATCGGAGAAACTCATCCAGGAAATGACTGTGACCTGGGAGGAGAAACTAAGGAAAACTGAGGAG ATTGCGCAGGAGCGGCAAAAGCAGCTTGAGAGCCTTGGCATATCTCTTCAGACTTCTGGAATCAAGGTTG GGGATGACAAGTGCTTCCTGGTTAACCTGAATGCTGACCCAGCTCTGAATGAACTCCTGGTGTACTACTT AAAGGAGCACACGCTGATAGGGTCGGCCAATTCTCAGGACATCCAGCTCTGCGGGATGGGCATCCTCCCT GAGCACGGCATCATAGACATCACGCCCGAGGGCCAGGTTGTGCTGACTCCTCAGAAGAACACCAGAACAT TTGTCAATGGGTCTTCTGTCTCCAGCCCCATCCAGCTGCACCATGGGGACAGGATACTGTGGGGAAACAA CCATTTCTTCAGACTCAATCTGCCAAAAAAGAAAAAGAAAGCAGAGCGAGAGGACGAGGACCGTGAGGCA TCCCTGAAAAACGACAGTAGTTCTGAGCAGCTGGATGCCGACGGCGACTCCTCCAGTGAGGTGTCCAGCG AGATCAACTTCAACTTTGAGTATGCACAGATGGAGGTGACCATGAAGGCTCTGGGCAGCAATGACCCAAT GCAGTCCATACTGAGCAGCCTAGAACAGCAGCATGAGGAGGAGAAGCGGTCTGCTCTGGAGCGCCAGAGG CTCATGTACGAACACGAACTGGAACAGCTTCGAAGAAGGCTGTCTCCTGAGAGGCAGAACTGCCGCGGTG TGGACCGGCTCTCCTTCCACTCGCCCAGTGCCCAGCAGCGCTTGAAACAGTGGGCTGAAGAGAGAGAAGC AACATTGAATAACAGCTTGATGAGGCTGAGGGAGCAGATTGTAAAGGCCAACCTGCTGGTTCGGGAAGCT AGTTACATCGCAGAAGAGTTGGATAAAAGAACCGAGTATAAAGTTACCCTCCAGATTCCAACCTCCAGTC TGGATGCCAATAGGAAGCGAGGCTCTCTCCTCAGTGAACCTGCCATCCAGGTGAGAAGAAAGGGCAAAGG AAAGCAGATTTGGTCTTTGGAAAAGCTGGAAAACAGGCTTTTGGATATGAGAGACCTCTACCAGGAGTGG AAGGAGTGTGAAGAAGACAGCCCGGTTAGCCGGTCCTACTTCAAGCGTGCTGACCCATTCTATGATGAGC AGGAAAACCATAGTCTCATCGGTGTGGCCAATGTCTTCCTGGAGACCCTGTTTTATGACGTGAAGTTACA GTATGCTGTGCCGATCATCAACCAGAAGGGAGAGGTGGCAGGCCGGCTGCACGTGGAGGTGATGCGACTC AGTGGGGCTATTGGGGAGAGGATTGCAGGAGGAGACGATCCTACAGAGGTCAGCTCTGAAAAGGAAGCCC AGGAGAACAGGCTGGTCTGCATGGTTAAAATACTCCAAGCCACAGGGTTGCCGCAGCATCTGTGTCACTT TGTCTTCTGCAAATATGACTTCTGGGATCAGCAGGAGCCAGTGACCGTTGCCCCTGAAGTGGATACGTCT TCGTCTCCCACCAGCAAGCAGCCACAGTGTATGGTTGTGTTCGATCACTGCAGTGAGTTCAGTGTTAACA TCACAGAAGACTTCATTGAATATCTCTCAGAAGGGGCCCTAGCCATTGAAGTATATGGACATAAAATGAA TGATCCCCGGAAAAACCCAGCCCTGTGGGACTTGGGGATCATCCAAGCAAAAACAAGGAGTCTTCGGGAC AGGTGGAGTGAAGTCACCAGAAAGTTGGAATTCTGGGTTCAGATCTTGGAACAGAATGAGAATGGTGATT ACTGTCCGGTGGAAGTAATTGCTGCAAAAGATGTCCCCACAGGAGGGATCTTCCAGCTCCGACAGGGGCA GTCTCGGCGAGTTCAAGTTGAAGTGAAGTCTGTGCAGGAGTCAGGAACTTTACCACTGATGGAAGAGTGC ATATTGTCAGTTGGCATTGGGTGTGTGAAAGTTAGGCCACTCCGGTCCCCTAAGATCCACGAGAATGTCC ATGAGGAAGAGGAGGACATGGACAGCTACCAGGATAGGGATTTAGAGCGGCTTCGTAGAAAATGGCTCAA TGCATTAACCAAACGTCAAGAATACTTGGATCAACAACTGCAGAAGCTTGTCAGTAAACATGACAAAACT GAGGATGATGCTGACCGAGAGGCACAGCTCCTGGAGATGCGGCTGACGCTCACAGAGGAAAGAAATGCAG TGATGGTGCCTTCTGCTGGGAGTGGCATTCCAGGGGCCCCTGCAGAATGGACCCCAGTGCCTGGGATGGA GACCCACATTCCTGTTATATTTCTTGACTTAAATGCGGATGATTTCAGCTCTCAGGATAATCTCGATGAC CCAGAAGCAGGATGGGATGCTACCCTGACTGGGGAAGAGGAGGAGGAGTTCTTTGAGCTGCAGATTGTGA AACAACACGATGGAGAGGTGAAGGCAGAGGCCTCCTGGGACTCTGCAGTGCACAGCTGTCCTCAACTCAG TAAGGGCACACCTGCTGACGAGCGAGTGTTCCTCATCCTGCGAGTGACTGTCCAGCTCAGCCATCCTGCC GACATGCAGCTGGTCCTACGCAAGAGGATTTGTGTCCATGTTCATGGGCGCCAGGGCTTTGCTCAAAGTC TCCTCAAAAAGATGTCTCACCGGAGCTCTATTCCTGGCTGTGGAGTGACCTTTGAAATCGTCTCCAATAT TCCAGAGGATGCCCAGGGAGTGGAGGAGCGAGAGGCACTGGCAAGGATGGCAGCTAACGTGGAAAACCCA GCATCTGCTGACTCCGAGGCCTATATTGAGAAGTACTTGAGGAGTGTGCTGGCCGTGGAAAACCTCCTGA CTTTAGATCGCCTGCGTCAGGAAGTGGCCGTGAAGGAGCAGCTGACGGGGAAGGGGAAGCTGAGCCGAAG GAGTATCAGCTCCCCCAGCATGAACAGATTGTCAGGAAGCCGGCAGGAGCTTAGCCCATCCCACAGTCTA GGCAGCAACAAGGGCCGGTGGGAAAGTCAACAGGATGTGTCCCAAACACTAGTTTCCAGAGGAATAGCTC CAGGCCCTCCAGCTCTCTCTGTTTCGCCCCAAAATAACCAGTCTCCTGATCCTGGACTGGGAGGCGTGGC AGCCTCTTACTTGAACCCAGTGAAGTCCCTGGTGCCACAGATGCCAAAGCTGCTGAAGTCTCTCTTCCCT GTTCGGGATGACAGAAGGGGGAGACACTCATCTCCCCTTGCACACCAGCCCGTGCCCCGAATCTTGGTGC AGCCTACCTTCTCAGATACCTGGGCAACAAGGACAGAGGAGGTGAGTCACCAGGTTGGGGCTGCCCCACT TGCAGAGGTGATGGCGGCCCCTGCAGTAAAGATCTGTGACAAACCTGTCAAAGTGTCTTCCCCTCCATCT CCCATGGTGGTTACTCAGCCCCCAGAAGGACAGGATGGACCACCCAGCCCTTTGAGTGAAGCTTCAAGTG GCTACTTCTCTCACAGTGTCTCTTCTGCCACCCTGTCAGAAACACTCACCTTAGGCCTGGACACCACAGG CCTCGGCAGCCAGACGCCTGGGTCCCCCCCTGCCCTGTGTCAGGTCACCCCAGAGCCTGAGCTGGCTTTC CTCTCCTGCACACAAAGCCATCCTCCTGCCCCAGAGGAGGCCCACGTACCTGCAGCTCCTACTCAAAGCA CAGAGCTGGAGGTTCCCAGAGCACCTCTTCTTTCTGAACCCGCGTCTGCTGTACCCACGTCCCCATTCAG AATCCGGAAAGTGCGGACCTCAGAGCTTAAGTCCTTCACAGGCATGCTGGGTGGTGCCTCCTCCGGAGCA GAGGAGGACCCCCTGGCTTCAGAAGACCCCAGCAATGCTAGGGGACAGACTCTGGGGAGGCTAGAAGTAA CATCTGACTCTGAGGATGCCAGCGAAGTCCCTGAGTGGCTCAGAGAGGGAGAGTATGTTGTTGTGGGCAC CAACAAGACGGGCATTGTACGGTATATTGGACCAACTGATTTCCAGGAGGGAACATGGATTGGTGTGGAG CTGGATCTGCCTGCAGGAAAGAATGATGGCTCCATTGGTGGGAAACAGTATTTCAGGTGCAACCCTGGCT ATGGGCTGCTGGTGAGGCCCAGCCGGGTCCGCAGGGCTGTGGGCACTGGGCGCAGGCGCAGTTCAGGGCT CCAGCCTCAGGGAGCCCCAGAGGTCCGCAGAAGTGCTACCATCTCGGGCTCTGCCACCAACCTGGCTTCA CTGACAGCTGCACTGGCCAAGGGAGACAGGAGTTACAAGAATCCTGAGAACCGGAAGTCCTGGGCCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR221195 representing NM_001081177 Red=Cloning site Green=Tags(s) MGDSKVKVAVRVRPMNRREIDLHTKCVVDVEANKVILNPVNTNLSKGDARGQPKIFAYDHCFWSMDESVR EKYAGQEDVFKCLGENILQNAFDGYNACIFAYGQTGSGKSYTMMGTADQPGLIPRLCSGLFERTQKEENE EQSFKVEVSYMEIYNEKVRDLLDPKGSRQTLKVREHSVLGPYVDGLSKLAVTSYKDIESLMSEGNKSRTV AATNMNEESSRSHAVFKITLTHTLYDVKSGTSGEKVGKLSLVDLAGSERATKTGAAGDRLKEGSNINKSL TTLGLVISALADQGAGKNKNKFVPYRDSVLTWLLKDSLGGNSKTAMVATVSPAADNYDETLSTLRYADRA KHIVNHAVVNEDPNARIIRDLREEVEKLREQLTKAEAMKSPELKDRLEESEKLIQEMTVTWEEKLRKTEE IAQERQKQLESLGISLQTSGIKVGDDKCFLVNLNADPALNELLVYYLKEHTLIGSANSQDIQLCGMGILP EHGIIDITPEGQVVLTPQKNTRTFVNGSSVSSPIQLHHGDRILWGNNHFFRLNLPKKKKKAEREDEDREA SLKNDSSSEQLDADGDSSSEVSSEINFNFEYAQMEVTMKALGSNDPMQSILSSLEQQHEEEKRSALERQR LMYEHELEQLRRRLSPERQNCRGVDRLSFHSPSAQQRLKQWAEEREATLNNSLMRLREQIVKANLLVREA SYIAEELDKRTEYKVTLQIPTSSLDANRKRGSLLSEPAIQVRRKGKGKQIWSLEKLENRLLDMRDLYQEW KECEEDSPVSRSYFKRADPFYDEQENHSLIGVANVFLETLFYDVKLQYAVPIINQKGEVAGRLHVEVMRL SGAIGERIAGGDDPTEVSSEKEAQENRLVCMVKILQATGLPQHLCHFVFCKYDFWDQQEPVTVAPEVDTS SSPTSKQPQCMVVFDHCSEFSVNITEDFIEYLSEGALAIEVYGHKMNDPRKNPALWDLGIIQAKTRSLRD RWSEVTRKLEFWVQILEQNENGDYCPVEVIAAKDVPTGGIFQLRQGQSRRVQVEVKSVQESGTLPLMEEC ILSVGIGCVKVRPLRSPKIHENVHEEEEDMDSYQDRDLERLRRKWLNALTKRQEYLDQQLQKLVSKHDKT EDDADREAQLLEMRLTLTEERNAVMVPSAGSGIPGAPAEWTPVPGMETHIPVIFLDLNADDFSSQDNLDD PEAGWDATLTGEEEEEFFELQIVKQHDGEVKAEASWDSAVHSCPQLSKGTPADERVFLILRVTVQLSHPA DMQLVLRKRICVHVHGRQGFAQSLLKKMSHRSSIPGCGVTFEIVSNIPEDAQGVEEREALARMAANVENP ASADSEAYIEKYLRSVLAVENLLTLDRLRQEVAVKEQLTGKGKLSRRSISSPSMNRLSGSRQELSPSHSL GSNKGRWESQQDVSQTLVSRGIAPGPPALSVSPQNNQSPDPGLGGVAASYLNPVKSLVPQMPKLLKSLFP VRDDRRGRHSSPLAHQPVPRILVQPTFSDTWATRTEEVSHQVGAAPLAEVMAAPAVKICDKPVKVSSPPS PMVVTQPPEGQDGPPSPLSEASSGYFSHSVSSATLSETLTLGLDTTGLGSQTPGSPPALCQVTPEPELAF LSCTQSHPPAPEEAHVPAAPTQSTELEVPRAPLLSEPASAVPTSPFRIRKVRTSELKSFTGMLGGASSGA EEDPLASEDPSNARGQTLGRLEVTSDSEDASEVPEWLREGEYVVVGTNKTGIVRYIGPTDFQEGTWIGVE LDLPAGKNDGSIGGKQYFRCNPGYGLLVRPSRVRRAVGTGRRRSSGLQPQGAPEVRRSATISGSATNLAS LTAALAKGDRSYKNPENRKSWAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001081177
ORF Size	5529 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001081177.2
RefSeq Size	5603 bp
RefSeq ORF	5532 bp
Locus ID	16554
MW	205 kDa

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