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Bbs4 (NM_175325) Mouse Tagged ORF Clone
CAT＃: MR221163
- TrueORF®
Bbs4 (Myc-DDK-tagged) - Mouse Bardet-Biedl syndrome 4 (human) (Bbs4)
ORF Plasmid: tGFP
"NM_175325" in other vectors (4)
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CNY 3,880.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW537059; AW742241; D9Ertd464e |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR221163 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAAGTGAAGCTTGGGATGAAAACTCAGGTTCCTGCATCTGTTGAATCTCAAAAACCTAGGTCAA AAAAAGCTCCAGACTTCCCTATTGTGGAGAAACAGAACTGGCTGATCCATCTCCACTATATCCGGAAGGA TTATGAAGCTTGCAAGGCTGTGATCAAAGAACAGCTTCAGGAGACTCAGGGGCTATGTGAATATGCTATC TATGTCCAAGCACTGATTTTTCGCCTGGAAGGAAATATCCAAGAATCCCTAGAACTCTTTCAGACATGTG CTGTTCTCAGCCCACAGTGTGCTGATAATCTCAAGCAGGTGGCCAGATCTTTGTTTCTTCTGGGAAAACA CAAAGCTGCCACTGAAGTATATAATGAAGCAGCTAAACTTAACCAGAAAGACTGGGAGATCTGCCATAAC CTGGGAGTGTGCTACACTTACCTGAAGCAGTTCAACAAGGCACAAGACCAACTGCACAGTGCCCTCCAGC TTAACAAGCATGACCTGACTTACATAATGTTGGGGAAGATCCATTTACTGCAGGGAGACCTGGATAAAGC CATCGAGATCTACAAGAAAGCAGTAGAGTTCTCACCAGAAAATACTGAACTTCTTACAACTTTAGGATTG CTCTACTTGCAGCTTGGTGTGTACCAGAAGGCATTTGAACATCTTGGGAATGCACTGACCTACGACCCTG CCAACTACAAGGCCATCTTGGCAGCAGGCAGCATGATGCAGACTCATGGGGACTTTGATGTCGCCCTTAC CAAATACAGAGTTGTAGCCTGTGCTATTCCAGAAAGTCCTCCACTTTGGAATAACATTGGAATGTGTTTC TTTGGCAAGAAGAAATATGTGGCAGCTATCAGCTGCCTAAAACGAGCCAACTACTTGGCACCCTTCGACT GGAAGATTCTGTACAACCTGGGCCTTGTCCATTTGACTATGCAGCAGTATGCATCAGCATTCCATTTTCT CAGTGCAGCCATCAACTTCCAGCCAAAGATGGGGGAACTCTACATGCTCTTGGCTGTGGCTCTGACCAAC CTGGAAGATATAGAGAATGCCAGAAGAGCTTATGTAGAAGCTGTTCGCCTGGATAAGTGTAACCCTTTAG TAAACCTGAACTATGCTGTGCTGCTGTATAACCAGGGTGAGAAGAAGAGCGCCCTGGCTCAGTACCAGGA GATGGAGAAGAAGGTCAACTTTCTCAAGGACAACAGCCCTCTGGAATTTGACTCTGAGATGGTAGAGATG GCTCAGAAGTTGGGAGCTGCCCTTCAGGTGGGAGAGGCACTGGTCTGGACCAAACCAGTCAAAGATCCCA AGACAAAGCACCGGACCAATTCAGGCAGCAAATCTGCTACTCTCCAGCAGCCTCTGGGTTCCATTCAAGC TCTAGGACAGGCGATGTCTTCAGCAGCTGCATACAGAAAGATCCTTTCAGGTGCTGTAGGAGCCCAGCTC CCAAAGCCACCATCACTGCCACTGGAGCCAGAGCCAGAGCCCACTGTGGAAGCAAGTCCAACAGAAGCAT CAGAACAAAAGAAAGAAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR221163 protein sequence
Red=Cloning site Green=Tags(s) MAEVKLGMKTQVPASVESQKPRSKKAPDFPIVEKQNWLIHLHYIRKDYEACKAVIKEQLQETQGLCEYAI YVQALIFRLEGNIQESLELFQTCAVLSPQCADNLKQVARSLFLLGKHKAATEVYNEAAKLNQKDWEICHN LGVCYTYLKQFNKAQDQLHSALQLNKHDLTYIMLGKIHLLQGDLDKAIEIYKKAVEFSPENTELLTTLGL LYLQLGVYQKAFEHLGNALTYDPANYKAILAAGSMMQTHGDFDVALTKYRVVACAIPESPPLWNNIGMCF FGKKKYVAAISCLKRANYLAPFDWKILYNLGLVHLTMQQYASAFHFLSAAINFQPKMGELYMLLAVALTN LEDIENARRAYVEAVRLDKCNPLVNLNYAVLLYNQGEKKSALAQYQEMEKKVNFLKDNSPLEFDSEMVEM AQKLGAALQVGEALVWTKPVKDPKTKHRTNSGSKSATLQQPLGSIQALGQAMSSAAAYRKILSGAVGAQL PKPPSLPLEPEPEPTVEASPTEASEQKKEK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175325 |
| ORF Size | 1563 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175325.3, NP_780534.1 |
| RefSeq Size | 2542 bp |
| RefSeq ORF | 1563 bp |
| Locus ID | 102774 |
| UniProt ID | Q8C1Z7 |
| MW | 58.3 kDa |
| Gene Summary | The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly and its ciliary localization. Required for microtubule anchoring at the centrosome but not for microtubule nucleation. May be required for the dynein-mediated transport of pericentriolar proteins to the centrosome (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217495 | Bbs4 (untagged) - Mouse Bardet-Biedl syndrome 4 (human) (Bbs4), (10ug) |
CNY 6,750.00 |
|
| MG221163 | Bbs4 (tGFP-tagged) - Mouse Bardet-Biedl syndrome 4 (human) (Bbs4), (10ug) |
CNY 5,040.00 |
|
| MR221163L3 | Lenti ORF clone of Bbs4 (Myc-DDK-tagged) - Mouse Bardet-Biedl syndrome 4 (human) (Bbs4) |
CNY 6,460.00 |
|
| MR221163L4 | Lenti ORF clone of Bbs4 (mGFP-tagged) - Mouse Bardet-Biedl syndrome 4 (human) (Bbs4) |
CNY 6,460.00 |
