Bbs4 (NM_175325) Mouse Untagged Clone

CAT#: MC217495

Bbs4 (untagged) - Mouse Bardet-Biedl syndrome 4 (human) (Bbs4), (10ug)



  "NM_175325" in other vectors (4)

CNY 6,750.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AW537059; AW742241; D9Ertd464e
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC217495 representing NM_175325
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGAAGTGAAGCTTGGGATGAAAACTCAGGTTCCTGCATCTGTTGAATCTCAAAAACCTAGGTCAA
AAAAAGCTCCAGACTTCCCTATTGTGGAGAAACAGAACTGGCTGATCCATCTCCACTATATCCGGAAGGA
TTATGAAGCTTGCAAGGCTGTGATCAAAGAACAGCTTCAGGAGACTCAGGGGCTATGTGAATATGCTATC
TATGTCCAAGCACTGATTTTTCGCCTGGAAGGAAATATCCAAGAATCCCTAGAACTCTTTCAGACATGTG
CTGTTCTCAGCCCACAGTGTGCTGATAATCTCAAGCAGGTGGCCAGATCTTTGTTTCTTCTGGGAAAACA
CAAAGCTGCCACTGAAGTATATAATGAAGCAGCTAAACTTAACCAGAAAGACTGGGAGATCTGCCATAAC
CTGGGAGTGTGCTACACTTACCTGAAGCAGTTCAACAAGGCACAAGACCAACTGCACAGTGCCCTCCAGC
TTAACAAGCATGACCTGACTTACATAATGTTGGGGAAGATCCATTTACTGCAGGGAGACCTGGATAAAGC
CATCGAGATCTACAAGAAAGCAGTAGAGTTCTCACCAGAAAATACTGAACTTCTTACAACTTTAGGATTG
CTCTACTTGCAGCTTGGTGTGTACCAGAAGGCATTTGAACATCTTGGGAATGCACTGACCTACGACCCTG
CCAACTACAAGGCCATCTTGGCAGCAGGCAGCATGATGCAGACTCATGGGGACTTTGATGTCGCCCTTAC
CAAATACAGAGTTGTAGCCTGTGCTATTCCAGAAAGTCCTCCACTTTGGAATAACATTGGAATGTGTTTC
TTTGGCAAGAAGAAATATGTGGCAGCTATCAGCTGCCTAAAACGAGCCAACTACTTGGCACCCTTCGACT
GGAAGATTCTGTACAACCTGGGCCTTGTCCATTTGACTATGCAGCAGTATGCATCAGCATTCCATTTTCT
CAGTGCAGCCATCAACTTCCAGCCAAAGATGGGGGAACTCTACATGCTCTTGGCTGTGGCTCTGACCAAC
CTGGAAGATATAGAGAATGCCAGAAGAGCTTATGTAGAAGCTGTTCGCCTGGATAAGTGTAACCCTTTAG
TAAACCTGAACTATGCTGTGCTGCTGTATAACCAGGGTGAGAAGAAGAGCGCCCTGGCTCAGTACCAGGA
GATGGAGAAGAAGGTCAACTTTCTCAAGGACAACAGCCCTCTGGAATTTGACTCTGAGATGGTAGAGATG
GCTCAGAAGTTGGGAGCTGCCCTTCAGGTGGGAGAGGCACTGGTCTGGACCAAACCAGTCAAAGATCCCA
AGACAAAGCACCGGACCAATTCAGGCAGCAAATCTGCTACTCTCCAGCAGCCTCTGGGTTCCATTCAAGC
TCTAGGACAGGCGATGTCTTCAGCAGCTGCATACAGAAAGATCCTTTCAGGTGCTGTAGGAGCCCAGCTC
CCAAAGCCACCATCACTGCCACTGGAGCCAGAGCCAGAGCCCACTGTGGAAGCAAGTCCAACAGAAGCAT
CAGAACAAAAGAAAGAAAAATAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_175325
Insert Size 1563 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_175325.3, NP_780534.1
RefSeq Size 2542 bp
RefSeq ORF 1563 bp
Locus ID 102774
UniProt ID Q8C1Z7
Gene Summary The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly and its ciliary localization. Required for microtubule anchoring at the centrosome but not for microtubule nucleation. May be required for the dynein-mediated transport of pericentriolar proteins to the centrosome (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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