Ptpn13 (NM_011204) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI324989; PTP-BL; PTPL1; Ptpri; RIP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR221075 representing NM_011204 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATGTGTCACTGGCAGAAGCCCTGGAGGTTCGGGGTGGACCTCTGCAGGAGGAGGAAATATGGGCTG TATTAAATCAGAGTGCTGAAAGCCTGCAAGAAGTATTTAGAAGAGTGAGCATTGCTGACCCTGCTGCGCT GGGCTTCATCATCTCTCCATGGTCGCTGCTGTTGCTGCCATCGGGCAGTGTGTCCTTTACGGATGAAAAT GTCTCCAATCAGGACCTTAGAGCATTCACCGCACCGGAGGTTCTTCAGAGCCATTCTCTGACTTCTCTCG CAGATGTAGAGAAGATCCATATTTATTCTCTTGGAATGACATTGTATTGGGGGGCTGACCATGAAGTACC TCAAAGCCAGCCCATCAAGCTGGGAGACCATCTTAATAGCATCCTGCTGGGCATGTGTGAGGATGTCATC TATGCTCGGGTGTCGGTTCGGACCGTCCTGGATGCTTGCAGTGCCCACATCAGGAACAGCAACTGCGCGC CTTCGTTTTCCTATGTGAAACAGTTGGTAAAGCTGGTTCTGGGCAATATTTCTGGGACGGATCCACTTTC CCGTAGCAGTGAACAAAAACCTGACCGGAGCCAGGCCATTCGAGACCGACTGAGAGGAAAAGGATTGCCC ACAGGAAGAAGCTCCACTTCAGACGCACTAGACACACACGAGGCTCCACTCTCTCAGCAGACCTTTCTTA ACAAAGGGCTTAGTAAGTCTATGGGATTTCTGTCCATCAGAGACACACGAGATGAGGAAGACTATCTCAA GGATACTCCATCTGATAATAATTCCAGGCATGAAGATTCTGAGACTTTCTCTTCCCCTTACCAGTTCAAA ACTAGTACTCCACAGATGGATGCACTCTCCAAGAAGAAGACATGGGCTTCCTCCATGGACTTGCTCTGTG CAGCTAACAGAGACATTTCTGGAGAGACGGGCAGGTACCAACGCTGTGACCCTGAGACAGTGACAGGACG GACGTCCATCACTCCTAGAAAAAAAGAAGGAAGGTACTCTGATGGAAGCATTGCCTTGGATATCTTTGGC CCTCAGAAGGTGGAGCCGGTGATCCACACCCGAGAACTGCCGACCTCCACCGCGGTATCAAGCGCTTTGG ACCGAATTCGAGAGAGACAAAAGAAACTTCAGGTTCTGAGAGAAGCCATGAATGTAGAAGAGCCAGTTCG AAGATACAAAACTTACCACAGCGATATCTTTAGTATTTCCAGTGAAAGCCCATCTGTTATTTCCTCGGAA TCAGACTTCAGACAAGTAAGAAAAAGTGAAGCTTCCAAGAGGTTTGAATCCAGCAGTGGTCTTCCCGGAG TAGATGAAACTGGCCAGACACGACCAAGTAGACAATATGAAACATCCCTCGAAGGCAACTTGATCAATCA GGACATAATGCTAAGGCGTCAAGAGGAAGAAATGATGCAGCTGCAAGCCAGGATGGCTCTCCGACAGTCG CGGCTCAGCCTGTATCCAGGGGACACCGTCAAAGCTTCCATGCTCGACATCTCTAGAGACCCCCTAAGGG AAATGGCCCTGGAGACAGCCATGACCCAGAGGAAGCTCAGGAATTTCTTTGGACCTGAGTTTGTGAAGAT GACGGTTGAGCCATTTGTATCTTTGGATTTGCCACGGTCTATCCTCTCGCAGACCAAGAAGGGGAAGAGT GAGGACCAGCGGCGGAAAGTGAACATAAGGCTCCTGAGCGGGCAGCGGCTGGAGCTCACCTGCGACACCA AGACCATCTGCAAGGATGTGTTCGACATGGTGGTGGCGCACATCGGCTTGGTGGAGCACCATTTGTTTGC TCTCGCCACCCGCAAAGAGAATGAATATTTCTTTGTTGATCCTGACTTAAAATTAACCAAAGTGGCCCCG GAGGGGTGGAAGGAAGAACCGAAGAGAAAGGGCAAGGCCGCCGTTGACTTTACGTTGTTTTTCCGAATTA AATTTTTCATGGATGATGTTAGTCTGATACAACATGACCTCACGTGCCATCAGTATTACCTTCAGCTGAG GAAAGACCTTCTGGATGAGAGGGTGCACTGTGACGACGAGGCCGCCCTGCTTCTTGCATCCTTGGCTCTC CAGGCAGAGTATGGAGATTATCAGCCAGAGGTTCATGGCGTGTCCTACTTTCGCCTGGAACATTACCTGC CCGCCAGGGTGATGGAGAAGCTGGACGTGTCCTACATTAAAGAAGAGTTACCCAAACTGCATAACACCTA TGCAGGCGCTTCCGAGAAGGAGACAGAGCTAGAGTTCTTAAAGGTCTGCCAAAGGCTGACAGAGTATGGA GTTCACTTTCACCGGGTACACCCTGAGAAGAAGTCACAGACAGGGATACTGCTGGGGGTCTGTTCCAAAG GTGTGCTGGTCTTTGAGGTTCACAATGGAGTCCGTGCCTTGGTCCTTCGCTTCCCGTGGAGGGAAACAAA AAAGATTTCCTTTTCAAAGAAGAAAATCACATTACAGAACACGTCAGATGGAATCAAGCATGCGTTCCAG ACGGACAGCAGCAAGGCATGCCAGTACCTGCTGCACCTGTGCTCCTCCCAGCACAAGTTCCAATTGCAGA TGAGGGCGCGGCAGAGCAACCAGGATGCCCAAGACATAGAGAGAGCTTCGTTTAGGAGCCTGAACCTGCA AGCAGAGTCTGTTAGAGGATTTAATATGGGCCGCGCAATCAGCACGGGCAGTCTGGCCAGCAGCACCATC AACAAACTTGCCGTCCGACCCCTGTCAGTTCAAGCTGAGATTCTGAAGAGGCTGTCCAGCTCAGAGTGGT CGCTTTACCAGCCCCTGCAGAACAGTTCAAAAGAGAAGACTGACAAAGCTTCCTGGGAGGAAAAGCCTAG AGGGATGAGCAAATCGTATCACGATCTCAGTCAGGCCTCTCTCTGTCCTCACCGGAAACAGGTCATTAAC ATGGAGGCCCTCCCACAAGCCTTTGCGGAGCTGGTGGGAAAACCCTTGTACCCGATGGCAAGATCTGACA CAGAGTCACTGGCAGGACTCCCAAAGCTTGATAATTCAAAGTCTGTTGCCAGTTTAAATAGAAGCCCCGA AAGGAGGAACCATGAATCAGACTCATCCACTGAAGACCCTGGCCAAGCATATGTTGTAGGAATGAGTTTG CCTAGCTCTGGAAAATCTTCATCCCAAGTCCCCTTTAAAGACAATGATACACTGCACAAAAGATGGAGCA TTGTTTCGTCACCAGAAAGGGAGATCACACTGGTGAACCTGAAGAAAGATCCAAAACACGGCTTAGGATT TCAAATTATCGGTGGGGAAAAAATGGGAAGACTGGATCTAGGAGTGTTTATCAGTGCAGTTACCCCTGGA GGACCTGCTGACCTGGATGGATGCTTAAAGCCTGGAGACCGTTTGATATCTGTGAACAGTGTGAGTCTGG AAGGGGTCAGTCACCACGCTGCAGTGGACATTCTGCAGAATGCACCTGAAGATGTGACACTTGTCATCTC CCAGCCAAAAGAAAAACCGTCCAAAGTGCCTTCTACTCCTGTTCATTTTGCCAATGGGATGAAAAGCTAC ACAAAGAAACCGGCATACATGCAGGACAGTGCTATGGACCCATCAGAGGACCAGCCCTGGCCACGGGGTA CTCTGAGGCACATCCCAGAGAGTCCCTTCGGGCTGTCTGGGGGCCTGCGGGAGGGAAGCCTCAGTTCCCA AGATTCCAGGACTGAAAGTGCCAGCCTATCCCAGAGCCAAGTCAACGGCTTCTTTGCCAGCCACTTAGGC GACAGAGGCTGGCAGGAACCACAGCATAGCAGCCCATCGCCATCTGTGACAACCAAGGTCAACGAGAAGA CCTTCTCCGACAGTAACCGAAGCAAGGCCAAGCGGCGAGGCATCTCCGATTTGATTGAACACCTCGACTG TGCTGATTCCGACAAAGACGATTCCACTTACACCAGCAGTCAAGACCATCAAACGTCAAAACAGGAACCT TCCTCCTCACTGAGTACATCGAACAAAACGAGCTTTCCAACCTCGTCTGCGTCACCTCCTAAGCCCGGAG ACACGTTTGAGGTTGAGCTGGCTAAAACTGATGGCAGCCTGGGGATAAGTGTCACGGGAGGTGTGAATAC CAGCGTCCGACATGGTGGTATTTATGTGAAAGCCATTATTCCCAAAGGAGCGGCAGAGTCAGATGGCAGA ATTCACAAAGGTGATCGTGTGCTGGCTGTTAATGGAGTCAGTCTGGAAGGAGCTACCCACAAGCAAGCTG TGGAAACGTTAAGAAACACAGGGCAGGTGGTCCATCTGTTGTTAGAAAAGGGGCAGGTGCCAACATCTAG GGAACGGGATCCTGCAGGCCCACAGAGCCCCCCTCCAGACCAAGACGCCCAAAGACAAGCCCCGGAAAAG GTGGCGAAGCAAACACCCCATGTCAAAGACTACAGCTTTGTTACTGAAGATAATACATTTGAGGTCAAGC TGTTTAAAAATAGCTCAGGCCTTGGATTCAGTTTTTCTCGGGAAGATAATCTTATACCAGAACAAATAAA TGGCAGCATAGTAAGGGTTAAAAAGCTCTTCCCTGGACAGCCGGCAGCAGAAAGTGGGAAAATCGATGTT GGAGATGTTATATTGAAAGTTAACGGAGCCCCCTTGAAGGGACTGTCTCAGCAGGACGTCATCTCTGCTC TCAGGGGAACAGCTCCAGAAGTGTCCTTGCTTCTCTGCAGACCGGCACCTGGTGTGCTACCAGAAATCGA TACTACATTTTTGAACCCACTCTACTCTCCAGCAAACTCATTTCTAAACAGCAGTAAAGAGACTTCGCAG CCATCATCCTCAGTAGAGCAGGGTGCCAGCTCGGACGACAATGGGGTGTCTGGCAAAACCAAGAACCATT GCAGGGCTCCATCCAGGAGAGAGAGTTACAGCGACCACAGCGAGAGTGGAGAGGATGACTCCGTGAGAGC ACCAGCCAAGATGCCAAATGTGACCCGAGTGGCAGCCTTTCCACACGAAGCACCGAGAAGCCAGGAAGAG TCCATATGTGCCATGTTTTACCTCCCTCGGAAAATACCCGGAAAGCTGGAGTCCGAGAGCAGCCATCCTC CTCCACTGGACGTGAGCCCTGGACAGACGTGCCAGCCCCCGGCAGAGTGTGCTCCGTCTGACGCCACTGG CAAACACTTTACACATCTTGCCTCTCAGCTGAGCAAAGAGGAAAACATAACAACCTTGAAAAATGACTTG GGAAACCACCTTGAGGATTCTGAGCTGGAAGTAGAGCTTCTCATTACCCTGGTTAAATCAGAAAAAGGAA GTCTGGGCTTTACAGTAACCAAAGGCAGTCAGAGTATTGGTTGTTATGTCCATGATGTCATACAGGATCC AGCCAAAGGTGACGGAAGGCTAAAGGCTGGAGACCGGCTTATAAAGGTTAATGATACAGACGTTACAAAC ATGACCCACACAGATGCAGTGAATCTGCTGCGAGCCGCACCCAAAACAGTCAGATTAGTTCTTGGGCGGA TTCTGGAGTTACCCAGGATGCCAGTATTTCCCCATCTGCTGCCCGACATTACAGTCACATGCCACGGAGA GGAGTTAGGATTTTCCCTGTCTGGAGGTCAAGGCAGTCCACATGGAGTGGTGTATATTAGTGATATCAAC CCAAGGTCAGCTGCAGCTGTTGACGGTAGTCTCCAGCTATTAGACATCATCCATTATGTGAACGGAGTCA GCACACAAGGAATGACCTTGGAAGATGCAAACAGGGCATTGGACCTGTCACTTCCTTCAGTGGTGCTGAA AGTAACAAGAGACGGTTGTCCAGTGGTGCCTACAACAAGAGCTGCCATTTCTGCGCCCAGGTTCACCAAA GCCAACGGCCTAACCAGCATGGAGCCTTCTGGACAGCCTGCACTCATGCCCAAGAACTCCTTCTCCAAGG TTAACGGGGAAGGAGTACATGAAGCTGTGTGCCCTGCAGGAGAAGGCTCGTCTTCTCAGATGAAGGAGTC AGCAGGCCTGACTGAAACCAAAGAATCTAACTCCCGGGATGATGACATCTATGATGATCCTCAAGAAGCT GAAGTTATCCAGTCCTTGCTGGATGTTGTGGACGAGGAAGCACAGAACCTCTTAAACCAGAGGCATGCCA CCAGAAGAGCCTGTTCTCCAGATCCACTGAGGACGAATGGGGAGGCACCAGAAGAAGGAGATACCGACTA TGATGGGTCTCCGCTACCTGAGGACGTCCCTGAGTCAGTGAGCAGCGGAGAAGGAAAAGTAGACCTTGCA AGCTTAACTGCAGCATCACAAGAAGAGAAGCCCATCGAAGAGGACGCCACGCAGGAAAGCAGAAACTCGA CAACGGAGACAACAGACGGTGAAGATTCCAGCAAAGATCCCCCCTTTCTGACGAATGAGGAGCTGGCAGC CCTGCCGGTGGTCAGAGTCCCTCCCTCTGGCAAGTACACGGGCACACAGCTGCAGGCCACCATCCGCACG CTGCAGGGCTTGCTCGACCAAGGGATCCCTTCTAAGGAGCTGGAGAACCTTCAAGAATTAAAACCTTTGG ATCAGTGTCTGATTGGACAGACAAAGGAAAACCGCAGGAAGAACAGATACAAAAATATCCTTCCCTATGA CACCACAAGAGTGCCTCTTGGAGATGAAGGTGGATACATCAATGCCAGTTTCATCAGGATCCCGGTGGGG ACCCAGGAATTCGTGTACATTGCCTGCCAGGGGCCGTTGCCCACCACTGTGGGGGATTTTTGGCAGATGG TTTGGGAGCAGAACTCCACGGTGATAGCAATGATGACGCAGGAGGTGGAAGGAGAAAAGATCAAATGCCA GCGGTACTGGCCAAGCATCCTGGGCACAACCACCATGGCCAACGAGAGGCTGCGCCTGGCCCTCCTGAGG ATGCAGCAGCTGAAGGGCTTCATCGTGCGGGTGATGGCCCTGGAGGATATTCAGACAGGGGAGGTGCGAC ATATTTCTCATCTGAATTTCACTGCCTGGCCAGACCACGATACACCGTCGCAGCCAGACGACCTGCTCAC ATTCATCTCCTACATGAGACACATCCGTAGGTCGGGCCCAGTCATCACACACTGCAGCGCTGGCATTGGA CGCTCAGGAACCCTCATTTGCATAGACGTGGTCCTAGGCTTAATCAGCCAGGATCTGGAATTCGACATCT CTGACCTAGTGCGCTGCATGAGGCTTCAGAGGCACGGCATGGTGCAGACCGAGGGTCAGTATGTCTTCTG CTATCAAGTCATCCTCTATGTCCTGACTCATCTTCAGGCTGAAGAGCAGAAGGCACAGCCAGGGCTCCCA CAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR221075 representing NM_011204 Red=Cloning site Green=Tags(s) MHVSLAEALEVRGGPLQEEEIWAVLNQSAESLQEVFRRVSIADPAALGFIISPWSLLLLPSGSVSFTDEN VSNQDLRAFTAPEVLQSHSLTSLADVEKIHIYSLGMTLYWGADHEVPQSQPIKLGDHLNSILLGMCEDVI YARVSVRTVLDACSAHIRNSNCAPSFSYVKQLVKLVLGNISGTDPLSRSSEQKPDRSQAIRDRLRGKGLP TGRSSTSDALDTHEAPLSQQTFLNKGLSKSMGFLSIRDTRDEEDYLKDTPSDNNSRHEDSETFSSPYQFK TSTPQMDALSKKKTWASSMDLLCAANRDISGETGRYQRCDPETVTGRTSITPRKKEGRYSDGSIALDIFG PQKVEPVIHTRELPTSTAVSSALDRIRERQKKLQVLREAMNVEEPVRRYKTYHSDIFSISSESPSVISSE SDFRQVRKSEASKRFESSSGLPGVDETGQTRPSRQYETSLEGNLINQDIMLRRQEEEMMQLQARMALRQS RLSLYPGDTVKASMLDISRDPLREMALETAMTQRKLRNFFGPEFVKMTVEPFVSLDLPRSILSQTKKGKS EDQRRKVNIRLLSGQRLELTCDTKTICKDVFDMVVAHIGLVEHHLFALATRKENEYFFVDPDLKLTKVAP EGWKEEPKRKGKAAVDFTLFFRIKFFMDDVSLIQHDLTCHQYYLQLRKDLLDERVHCDDEAALLLASLAL QAEYGDYQPEVHGVSYFRLEHYLPARVMEKLDVSYIKEELPKLHNTYAGASEKETELEFLKVCQRLTEYG VHFHRVHPEKKSQTGILLGVCSKGVLVFEVHNGVRALVLRFPWRETKKISFSKKKITLQNTSDGIKHAFQ TDSSKACQYLLHLCSSQHKFQLQMRARQSNQDAQDIERASFRSLNLQAESVRGFNMGRAISTGSLASSTI NKLAVRPLSVQAEILKRLSSSEWSLYQPLQNSSKEKTDKASWEEKPRGMSKSYHDLSQASLCPHRKQVIN MEALPQAFAELVGKPLYPMARSDTESLAGLPKLDNSKSVASLNRSPERRNHESDSSTEDPGQAYVVGMSL PSSGKSSSQVPFKDNDTLHKRWSIVSSPEREITLVNLKKDPKHGLGFQIIGGEKMGRLDLGVFISAVTPG GPADLDGCLKPGDRLISVNSVSLEGVSHHAAVDILQNAPEDVTLVISQPKEKPSKVPSTPVHFANGMKSY TKKPAYMQDSAMDPSEDQPWPRGTLRHIPESPFGLSGGLREGSLSSQDSRTESASLSQSQVNGFFASHLG DRGWQEPQHSSPSPSVTTKVNEKTFSDSNRSKAKRRGISDLIEHLDCADSDKDDSTYTSSQDHQTSKQEP SSSLSTSNKTSFPTSSASPPKPGDTFEVELAKTDGSLGISVTGGVNTSVRHGGIYVKAIIPKGAAESDGR IHKGDRVLAVNGVSLEGATHKQAVETLRNTGQVVHLLLEKGQVPTSRERDPAGPQSPPPDQDAQRQAPEK VAKQTPHVKDYSFVTEDNTFEVKLFKNSSGLGFSFSREDNLIPEQINGSIVRVKKLFPGQPAAESGKIDV GDVILKVNGAPLKGLSQQDVISALRGTAPEVSLLLCRPAPGVLPEIDTTFLNPLYSPANSFLNSSKETSQ PSSSVEQGASSDDNGVSGKTKNHCRAPSRRESYSDHSESGEDDSVRAPAKMPNVTRVAAFPHEAPRSQEE SICAMFYLPRKIPGKLESESSHPPPLDVSPGQTCQPPAECAPSDATGKHFTHLASQLSKEENITTLKNDL GNHLEDSELEVELLITLVKSEKGSLGFTVTKGSQSIGCYVHDVIQDPAKGDGRLKAGDRLIKVNDTDVTN MTHTDAVNLLRAAPKTVRLVLGRILELPRMPVFPHLLPDITVTCHGEELGFSLSGGQGSPHGVVYISDIN PRSAAAVDGSLQLLDIIHYVNGVSTQGMTLEDANRALDLSLPSVVLKVTRDGCPVVPTTRAAISAPRFTK ANGLTSMEPSGQPALMPKNSFSKVNGEGVHEAVCPAGEGSSSQMKESAGLTETKESNSRDDDIYDDPQEA EVIQSLLDVVDEEAQNLLNQRHATRRACSPDPLRTNGEAPEEGDTDYDGSPLPEDVPESVSSGEGKVDLA SLTAASQEEKPIEEDATQESRNSTTETTDGEDSSKDPPFLTNEELAALPVVRVPPSGKYTGTQLQATIRT LQGLLDQGIPSKELENLQELKPLDQCLIGQTKENRRKNRYKNILPYDTTRVPLGDEGGYINASFIRIPVG TQEFVYIACQGPLPTTVGDFWQMVWEQNSTVIAMMTQEVEGEKIKCQRYWPSILGTTTMANERLRLALLR MQQLKGFIVRVMALEDIQTGEVRHISHLNFTAWPDHDTPSQPDDLLTFISYMRHIRRSGPVITHCSAGIG RSGTLICIDVVLGLISQDLEFDISDLVRCMRLQRHGMVQTEGQYVFCYQVILYVLTHLQAEEQKAQPGLP Q TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_011204
ORF Size	7353 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_011204.2, NP_035334.2
RefSeq Size	8298 bp
RefSeq ORF	7356 bp
Locus ID	19249
UniProt ID	Q64512
MW	270.7 kDa
Gene Summary	Tyrosine phosphatase which regulates negatively FAS-induced apoptosis and NGFR-mediated pro-apoptotic signaling. May regulate phosphoinositide 3-kinase (PI3K) signaling through dephosphorylation of PIK3R2.[UniProtKB/Swiss-Prot Function]

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