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Sema4f (NM_001113481) Mouse Tagged ORF Clone
CAT＃: MR221049
- TrueORF®
Sema4f (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain (Sema4f), transcript variant 2
ORF Plasmid: tGFP
"NM_001113481" in other vectors (4)
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CNY 5,510.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR221049 representing NM_001113481
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGCCAGGGCCGAGCGGCCCCGCCCGGGCCCCCGGCCGCCTCCGGTCTCTCTCTTCCCGCCGCCGT CGTCGCTGCTGCTGCTGCTGCTGGCGATGCTGAGCGCCCCGGTGTGCGGCCGCGTCCCCCGCTCGGTGCC CAGAACCTCGCTGCCCATCTCCGAGGCTGACTCCTATCTCACCCGGTTTGCGGCCCCTCATACGTACAAT TACTCTGCTCTCCTGGTGGATCCTGCTTCTCACACACTTTACGTCGGTGCACGGGACAGCATCTTCGCTT TAACCCTGCCCTTCTCTGGGGAAAAGCCTCGAAGGATCGACTGGATGGTGCCCGAGACTCACAGACAGAA CTGTAGGAAGAAAGGCAAGAAAGAGGACGAATGTCACAATTTTATCCAGATTCTCGCCATTGCCAATGCC TCTCACCTCCTCACGTGTGGCACCTTCGCTTTTGATCCGAAGTGCGGGGTTATTGATGTGTCCAGTTTCC AGCAGGTTGAAAGACTTGAGAGTGGCCGGGGGAAATGTCCTTTTGAGCCAGCTCAACGGTCAGCAGCTGT AATGGCTGGGGGCGTCCTCTACACCGCCACTGTGAAAAACTTCCTGGGGACAGAGCCGATTATCTCCCGA GCTGTGGGTCGAGCTGAGGACTGGATTCGAACAGAGACCTTGTCATCCTGGCTTAATGCCCCAGCCTTTG TCGCAGCTATGGTCCTGAGCCCGGCTGAGTGGGGGGATGAAGATGGAGACGATGAAATCTTCTTTTTCTT CACGGAGACCTCCCGAGTCTTGGACTCATATGAGCGCATCAAGGTCCCACGAGTGGCCCGTGTGTGTGCG GGGGACCTTGGGGGCCGGAAGACCCTTCAGCAGAGATGGACGACGTTTCTAAAGGCTGACCTGCTGTGTC CAGGGCCCGAGCATGGAAGGGCCTCGGGGGTTCTGCAGGATATGACAGAGCTTCGACCTCAGCCTGGCGC GGGGACCCCCCTCTTTTATGGCATCTTTTCCTCCCAGTGGGAAGGAGCCGCCATTTCTGCTGTGTGTGCC TTCCGACCCCAAGACATCCGGGCAGTGCTGAATGGTCCCTTTAGAGAGCTAAAACATGACTGCAACAGGG GACTACCTGTCATGGACAACGAGGTGCCCCAGCCCAGACCTGGAGAGTGCATCACCAACAACATGAAGTT CCAGCAGTTTGGATCCTCACTCTCCCTGCCAGACCGTGTGCTCACCTTTATCAGAGACCACCCGCTCATG GACAGGCCCGTGTTTCCAGCTGATGGCCGCCCCCTGCTGGTCACTACGGATACAGCCTATCTCAGAGTTG TGGCTCACCGGGTGACCAGCCTCTCAGGGAAAGAATATGATGTGCTCTACCTGGGGACAGAGGATGGGCA CCTCCATCGGGCTGTGCGCATCGGAGCTCAGCTCAGTGTCCTGGAGGATCTGGCCTTGTTCCCGGAAACA CAGCCGGTTGAAAGCATGAAATTGTACCATGATTGGCTTCTGGTGGGCTCCCATACTGAGGTGACACAAG TGAACACCAGCAACTGTGGCCGTCTCCAGAGCTGCTCAGAATGTATCCTGGCCCAGGATCCCGTGTGTGC CTGGAGCTTCCGGCTCGATGCTTGTGTGGCCCATGCAGGCGAGCACCGTGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR221049 representing NM_001113481
Red=Cloning site Green=Tags(s) MLARAERPRPGPRPPPVSLFPPPSSLLLLLLAMLSAPVCGRVPRSVPRTSLPISEADSYLTRFAAPHTYN YSALLVDPASHTLYVGARDSIFALTLPFSGEKPRRIDWMVPETHRQNCRKKGKKEDECHNFIQILAIANA SHLLTCGTFAFDPKCGVIDVSSFQQVERLESGRGKCPFEPAQRSAAVMAGGVLYTATVKNFLGTEPIISR AVGRAEDWIRTETLSSWLNAPAFVAAMVLSPAEWGDEDGDDEIFFFFTETSRVLDSYERIKVPRVARVCA GDLGGRKTLQQRWTTFLKADLLCPGPEHGRASGVLQDMTELRPQPGAGTPLFYGIFSSQWEGAAISAVCA FRPQDIRAVLNGPFRELKHDCNRGLPVMDNEVPQPRPGECITNNMKFQQFGSSLSLPDRVLTFIRDHPLM DRPVFPADGRPLLVTTDTAYLRVVAHRVTSLSGKEYDVLYLGTEDGHLHRAVRIGAQLSVLEDLALFPET QPVESMKLYHDWLLVGSHTEVTQVNTSNCGRLQSCSECILAQDPVCAWSFRLDACVAHAGEHRG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001113481 |
| ORF Size | 1662 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001113481.1, NP_001106953.1 |
| RefSeq Size | 2735 bp |
| RefSeq ORF | 1665 bp |
| Locus ID | 20355 |
| MW | 61.7 kDa |
| Gene Summary | This gene encodes a member of semaphorin family of membrane-bound and secreted proteins that are involved in guiding axonal growth. The encoded protein is a transmembrane protein localized to the glutamatergic synapses via its association with a synapse-associated scaffolding protein. In oligodendrocyte precursor cells, the encoded protein contributes to the outward migration and differentiation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218959 | Sema4f (untagged) - Mouse sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain (Sema4f), transcript variant 2, (10ug) |
CNY 7,130.00 |
|
| MG221049 | Sema4f (tGFP-tagged) - Mouse sema domain immunoglobulin domain (Ig) TM domain and short cytoplasmic domain (Sema4f) transcript variant 2, (10ug) |
CNY 6,080.00 |
|
| MR221049L3 | Lenti ORF clone of Sema4f (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain (Sema4f), transcript variant 2 |
CNY 7,410.00 |
|
| MR221049L4 | Lenti ORF clone of Sema4f (mGFP-tagged) - Mouse sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain (Sema4f), transcript variant 2 |
CNY 7,410.00 |
