Sema4f (NM_001113481) Mouse Untagged Clone

CAT#: MC218959

Sema4f (untagged) - Mouse sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain (Sema4f), transcript variant 2, (10ug)



  "NM_001113481" in other vectors (4)

CNY 7,130.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC218959 representing NM_001113481
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGGCCAGGGCCGAGCGGCCCCGCCCGGGCCCCCGGCCGCCTCCGGTCTCTCTCTTCCCGCCGCCGT
CGTCGCTGCTGCTGCTGCTGCTGGCGATGCTGAGCGCCCCGGTGTGCGGCCGCGTCCCCCGCTCGGTGCC
CAGAACCTCGCTGCCCATCTCCGAGGCTGACTCCTATCTCACCCGGTTTGCGGCCCCTCATACGTACAAT
TACTCTGCTCTCCTGGTGGATCCTGCTTCTCACACACTTTACGTCGGTGCACGGGACAGCATCTTCGCTT
TAACCCTGCCCTTCTCTGGGGAAAAGCCTCGAAGGATCGACTGGATGGTGCCCGAGACTCACAGACAGAA
CTGTAGGAAGAAAGGCAAGAAAGAGGACGAATGTCACAATTTTATCCAGATTCTCGCCATTGCCAATGCC
TCTCACCTCCTCACGTGTGGCACCTTCGCTTTTGATCCGAAGTGCGGGGTTATTGATGTGTCCAGTTTCC
AGCAGGTTGAAAGACTTGAGAGTGGCCGGGGGAAATGTCCTTTTGAGCCAGCTCAACGGTCAGCAGCTGT
AATGGCTGGGGGCGTCCTCTACACCGCCACTGTGAAAAACTTCCTGGGGACAGAGCCGATTATCTCCCGA
GCTGTGGGTCGAGCTGAGGACTGGATTCGAACAGAGACCTTGTCATCCTGGCTTAATGCCCCAGCCTTTG
TCGCAGCTATGGTCCTGAGCCCGGCTGAGTGGGGGGATGAAGATGGAGACGATGAAATCTTCTTTTTCTT
CACGGAGACCTCCCGAGTCTTGGACTCATATGAGCGCATCAAGGTCCCACGAGTGGCCCGTGTGTGTGCG
GGGGACCTTGGGGGCCGGAAGACCCTTCAGCAGAGATGGACGACGTTTCTAAAGGCTGACCTGCTGTGTC
CAGGGCCCGAGCATGGAAGGGCCTCGGGGGTTCTGCAGGATATGACAGAGCTTCGACCTCAGCCTGGCGC
GGGGACCCCCCTCTTTTATGGCATCTTTTCCTCCCAGTGGGAAGGAGCCGCCATTTCTGCTGTGTGTGCC
TTCCGACCCCAAGACATCCGGGCAGTGCTGAATGGTCCCTTTAGAGAGCTAAAACATGACTGCAACAGGG
GACTACCTGTCATGGACAACGAGGTGCCCCAGCCCAGACCTGGAGAGTGCATCACCAACAACATGAAGTT
CCAGCAGTTTGGATCCTCACTCTCCCTGCCAGACCGTGTGCTCACCTTTATCAGAGACCACCCGCTCATG
GACAGGCCCGTGTTTCCAGCTGATGGCCGCCCCCTGCTGGTCACTACGGATACAGCCTATCTCAGAGTTG
TGGCTCACCGGGTGACCAGCCTCTCAGGGAAAGAATATGATGTGCTCTACCTGGGGACAGAGGATGGGCA
CCTCCATCGGGCTGTGCGCATCGGAGCTCAGCTCAGTGTCCTGGAGGATCTGGCCTTGTTCCCGGAAACA
CAGCCGGTTGAAAGCATGAAATTGTACCATGATTGGCTTCTGGTGGGCTCCCATACTGAGGTGACACAAG
TGAACACCAGCAACTGTGGCCGTCTCCAGAGCTGCTCAGAATGTATCCTGGCCCAGGATCCCGTGTGTGC
CTGGAGCTTCCGGCTCGATGCTTGTGTGGCCCATGCAGGCGAGCACCGTGGGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001113481
Insert Size 1665 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001113481.1, NP_001106953.1
RefSeq Size 2735 bp
RefSeq ORF 1665 bp
Locus ID 20355
Gene Summary This gene encodes a member of semaphorin family of membrane-bound and secreted proteins that are involved in guiding axonal growth. The encoded protein is a transmembrane protein localized to the glutamatergic synapses via its association with a synapse-associated scaffolding protein. In oligodendrocyte precursor cells, the encoded protein contributes to the outward migration and differentiation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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