Camk2b (NM_007595) Mouse Tagged ORF Clone
CAT#: MR220945
- TrueORF®
Camk2b (Myc-DDK-tagged) - Mouse calcium/calmodulin-dependent protein kinase II, beta (Camk2b), transcript variant 2
ORF Plasmid: tGFP
"NM_007595" in other vectors (4)
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CNY 5,420.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CaMKII |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR220945 representing NM_007595
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCACGGTGACCTGCACCCGTTTCACCGACGAGTACCAGCTATACGAGGATATTGGCAAGGGGG CTTTCTCTGTGGTCCGACGCTGTGTCAAGCTCTGTACCGGCCATGAGTATGCAGCCAAGATCATTAATAC CAAGAAGCTGTCGGCCAGAGATCACCAGAAACTGGAGAGAGAAGCTCGGATCTGCCGCCTGCTGAAGCAT TCCAACATTGTACGCCTCCATGACAGCATCTCTGAAGAGGGCTTCCACTACCTGGTCTTCGATCTGGTCA CTGGTGGGGAGCTCTTTGAAGACATCGTGGCAAGAGAGTACTACAGTGAAGCTGATGCCAGTCACTGCAT CCAGCAGATCCTGGAAGCTGTTCTCCATTGTCACCAAATGGGGGTCGTCCACAGAGACCTCAAGCCTGAG AACCTGCTTCTGGCCAGCAAATGCAAAGGCGCCGCAGTGAAGCTGGCAGACTTCGGCCTGGCCATCGAGG TTCAGGGAGACCAGCAGGCATGGTTTGGATTTGCGGGAACGCCAGGCTACCTGTCTCCCGAGGTCCTTCG GAAGGAGGCCTACGGCAAACCTGTGGACATCTGGGCATGTGGGGTGATCCTGTATATCCTGCTGGTGGGC TACCCACCTTTCTGGGATGAGGACCAACACAAGCTGTACCAGCAGATCAAGGCTGGGGCGTATGATTTCC CATCCCCTGAGTGGGACACCGTTACTCCTGAAGCCAAAAACCTCATCAACCAGATGCTGACCATCAACCC TGCCAAGCGCATCACGGCCCATGAGGCCCTGAAGCACCCATGGGTCTGCCAACGTTCCACCGTGGCCTCT ATGATGCACAGACAGGAGACTGTGGAATGTCTGAAGAAGTTCAATGCAAGGAGGAAGCTCAAGGGAGCCA TCCTCACCACTATGCTGGCCACACGGAATTTCTCAGTGGGCAGACAGACCACCGCTCCGGCCACAATGTC CACCGCGGCCTCCGGCACCACCATGGGGCTGGTGGAACAAGCCAAGAGTTTACTCAACAAGAAAGCAGAT GGAGTCAAGCCCCAGACAAACAGCACCAAAAACAGCTCGGCCATCACCAGCCCCAAAGGATCTCTCCCTC CTGCTGCCCTGGAGCCTCAAACCACCGTTATCCATAACCCAGTGGACGGGATTAAGGAATCTTCCGACAG CACCAACACAACCATAGAGGATGAAGATGCCAAAGCCCGGAAGCAGGAAATCATCAAGACCACAGAGCAG CTCATTGAGGCCGTCAACAATGGGGACTTTGAGGCCTATGCGAAAATCTGTGACCCAGGCCTGACCTCAT TTGAGCCTGAAGCTCTGGGCAACCTGGTCGAAGGGATGGATTTCCACAGATTCTACTTTGAGAACCTGCT GGCCAAGAACAGCAAGCCGATCCACACCACCATCCTGAACCCGCACGTGCACGTCATTGGCGAGGATGCG GCATGCATCGCCTACATCCGCCTCACACAGTACATCGATGGCCAGGGCAGACCCCGTACCAGCCAGTCCG AAGAGACCCGTGTGTGGCACCGCCGCGACGGCAAGTGGCAGAATGTACATTTCCACTGCTCGGGCGCTCC AGTGGCCCCGCTGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR220945 representing NM_007595
Red=Cloning site Green=Tags(s) MATTVTCTRFTDEYQLYEDIGKGAFSVVRRCVKLCTGHEYAAKIINTKKLSARDHQKLEREARICRLLKH SNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPE NLLLASKCKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKEAYGKPVDIWACGVILYILLVG YPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITAHEALKHPWVCQRSTVAS MMHRQETVECLKKFNARRKLKGAILTTMLATRNFSVGRQTTAPATMSTAASGTTMGLVEQAKSLLNKKAD GVKPQTNSTKNSSAITSPKGSLPPAALEPQTTVIHNPVDGIKESSDSTNTTIEDEDAKARKQEIIKTTEQ LIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLLAKNSKPIHTTILNPHVHVIGEDA ACIAYIRLTQYIDGQGRPRTSQSEETRVWHRRDGKWQNVHFHCSGAPVAPLQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007595 |
ORF Size | 1626 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007595.5, NP_031621.3 |
RefSeq Size | 4025 bp |
RefSeq ORF | 1629 bp |
Locus ID | 12323 |
UniProt ID | P28652 |
MW | 60.9 kDa |
Gene Summary | Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca(2+) transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Also regulates the migration of developing neurons (PubMed:29100089). Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2 (PubMed:21752990).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Wip1 phosphatase modulates both long-term potentiation and long-term depression through the dephosphorylation of CaMKII
,He, ZY;Hu, WY;Zhang, M;Yang, ZZ;Zhu, HM;Xing, D;Ma, QH;Xiao, ZC;,
Cell Adh Migr
,PubMed ID 27158969
[CAMK2B]
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Documents
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC218144 | Camk2b (untagged) - Mouse calcium/calmodulin-dependent protein kinase II, beta (Camk2b), transcript variant 2, (10ug) |
CNY 6,940.00 |
|
MG220945 | Camk2b (tGFP-tagged) - Mouse calcium/calmodulin-dependent protein kinase II beta (Camk2b) transcript variant 2, (10ug) |
CNY 6,040.00 |
|
MR220945L3 | Lenti ORF clone of Camk2b (Myc-DDK-tagged) - Mouse calcium/calmodulin-dependent protein kinase II, beta (Camk2b), transcript variant 2 |
CNY 7,320.00 |
|
MR220945L4 | Lenti ORF clone of Camk2b (mGFP-tagged) - Mouse calcium/calmodulin-dependent protein kinase II, beta (Camk2b), transcript variant 2 |
CNY 7,320.00 |