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Nfasc (NM_182716) Mouse Tagged ORF Clone
CAT＃: MR220931
- TrueORF®
Nfasc (Myc-DDK-tagged) - Mouse neurofascin (Nfasc), transcript variant 1
ORF Plasmid: tGFP
"NM_182716" in other vectors (4)
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CNY 8,632.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA387016; D430023G06Rik; mKIAA0756; NF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR220931 representing NM_182716
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGGCAGCAGGCGCCACCCTGGGTCCACATAGCCCTCATCCTCTTCCTCCTCAGCCTCGGAGGGG CCATCGAGATTCCGATGGACCCAAGCATTCAGAATGAGCTGACCCAACCCCCAACTATCACCAAGCAGTC CGTGAAGGACCACATCGTGGACCCTCGAGATAACATCCTGATTGAATGTGAAGCTAAAGGCAACCCCGCC CCCAGTTTTCACTGGACTCGCAACAGCAGATTCTTCAACATTGCCAAGGACCCACGGGTGTCCATGAGGA GGAGATCTGGGACCTTGGTGATCGACTTCCGCAGTGGTGGGCGGCCTGAGGAATACGAAGGGGAGTACCA GTGCTTTGCCCGGAACAAATTTGGCACTGCACTTAGCAACCGCATCCGCCTGCAGGTGTCCAAATCTCCC CTGTGGCCCAAGGAAAACCTAGACCCCGTCGTGGTTCAAGAGGGTGCCCCCTTGACACTACAGTGCAACC CCCCACCCGGCCTCCCGTCCCCCGTCATCTTCTGGATGAGCAGCTCCATGGAGCCCATCACCCAGGACAA GCGTGTCTCCCAGGGTCACAACGGGGACCTGTACTTCTCCAACGTCATGCTGCAGGACATGCAGACCGAC TACAGCTGCAACGCGCGCTTTCACTTCACCCACACCATTCAGCAGAAGAACCCCTTCACCCTCAAGGTCC TCACCACCCGAGGAGTTGCAGAAAGAACGCCCAGCTTCATGTATCCCCAGGGCACATCGAGCAGTCAGAT GGTTCTCCGTGGCATGGACCTGCTGCTGGAATGCATTGCCTCTGGCGTCCCAACACCAGACATTGCATGG TACAAGAAAGGTGGGGACCTCCCATCTAACAAGGCCAAGTTCGAGAACTTTAATAAGGCTCTGCGCATCA CCAATGTCTCTGAAGAGGACTCTGGGGAGTATTTCTGCCTGGCCTCCAACAAGATGGGCAGCATCCGGCA CACGATCTCGGTGAGAGTAAAGGCTGCTCCATACTGGCTGGATGAGCCCAAGAACCTGATCCTGGCTCCT GGGGAAGATGGGAGGCTGGTATGCCGAGCCAATGGGAACCCGAAGCCGACCGTGCAGTGGATGGTGAATG GAGAGCCTTTACAATCGGCACCACCCAATCCCAACCGTGAGGTAGCTGGAGACACTATCATCTTCCGGGA TACTCAGATCAGCAGCAGGGCAGTGTACCAATGTAATACATCCAATGAACATGGCTACCTGCTGGCCAAT GCCTTCGTCAGCGTGTTAGATGTACCCCCTCGGATGCTGTCTGCCCGCAACCAGCTCATCAGGGTGATCC TTTATAACCGGACACGGCTGGACTGTCCGTTCTTTGGGTCTCCCATCCCAACACTCCGATGGTTTAAGAA TGGGCAAGGAAGCAACCTGGATGGCGGTAACTACCACGTCTACGAAAACGGCAGTCTAGAAATCAAGATG ATTCGCAAAGAGGACCAAGGCATCTACACCTGTGTGGCCACCAACATCCTGGGCAAAGCCGAAAATCAAG TCCGCCTGGAGGTCAAAGACCCCACCAGGATCTACAGGATGCCCGAGGACCAGGTGGCCAAGAGGGGCAC CACGGTGCAGCTGGAGTGCCGCGTGAAACATGACCCCTCCTTGAAGCTCACAGTCTCCTGGCTGAAGGAC GATGAGCCACTCTACATTGGAAACAGGATGAAGAAGGAAGATGACTCCCTGACGATCTTCGGAGTGGCAG AGCGGGACCAGGGCAGTTACACGTGTATGGCCAGCACCGAGCTGGACCAGGACCTGGCAAAGGCCTACCT CACTGTTCTAGCTGATCAGGCCACTCCAACTAACCGTTTGGCTGCCCTACCCAAAGGGCGACCAGACCGA CCCAGGGACCTGGAGCTCACTGACCTGGCTGAGAGGAGTGTGAGGCTGACCTGGATCCCAGGGGATGACA ACAACAGCCCTATCACAGACTACGTCGTTCAGTTTGAAGAGGACCAGTTCCAACCAGGGGTGTGGCATGA CCACTCCAGGTTCCCAGGCAGCGTCAACTCAGCCGTCCTCCATCTGTCCCCATATGTCAACTACCAATTC AGAGTCATCGCTGTCAACGAGGTTGGGAGCAGCCACCCCAGCCTTCCATCCGAGCGGTACCGAACCAGTG GGGCACCCCCTGAATCTAATCCCAGTGATGTGAAGGGCGAAGGGACAAGAAAGAACAATATGGAGATCAC GTGGACGCCTATGAATGCTACCTCTGCCTTTGGCCCCAACCTACGCTACATTGTCAAGTGGCGACGGAGA GAGACCCGAGAGACTTGGAACAATGTCACAGTGTGGGGCTCTCGCTACGTGGTGGGGCAGACGCCTGTCT ACGTTCCCTATGAGATCCGAGTCCAGGCTGAAAATGACTTTGGGAAAGGCCCCGAGCCTGACACCATCAT TGGGTACTCCGGAGAAGATTTACCCAGTGCCCCCAGGCGGTTCAGAGTCCGACAGCCCAACCTGGAGACC ATCAACCTGGAGTGGGACCACCCAGAGCACCCCAACGGAATCCTGATTGGATACATCCTCAGATACGTGC CCTTTAATGGAACCAAACTGGGAAAGCAGATGGTGGAAAACTTCTCTCCCAATCAGACCAAGTTCTCTGT GCAGAGAGCAGACCCAGTGTCGCGTTACCGCTTCTCCCTCAGTGCCAGGACACAGGTGGGCTCTGGAGAA GCAGCCACAGAGGAGTCCCCAGCACCTCCAAATGAAGCTACTCCAACTGCAGCTCCTCCCACGTTGCCCC CGACTACTGTGGGTACCACAGGCCTTGTGAGCAGTACTGATGCTACTGCCCTTGCTGCCACCAGTGAAGC CACAACAGTTCCCATCATTCCAACCGTCGTACCTACCACCGTCGCCACCACCATTGCCACAACTACTACA ACCACTGCCGCCACCACCACCACCACCACTACGGAGAGCCCTCCCACTACCACTGCTGGGACTAAGATTC ACGAAACCGCCCCCGACGAGCAGTCCATTTGGAACGTCACAGTGCTCCCCAACAGTAAATGGGCCAACAT CACCTGGAAGCACAATTTCAGGCCTGGAACTGACTTTGTGGTTGAGTACATCGACAGCAACCATACGAAA AAAACTGTCCCTGTTAAGGCCCAGGCCCAGCCTATACAGCTGACAGACCTCTTTCCCGGGATGACGTACA CGTTGCGGGTGTATTCCCGGGACAACGAGGGCATCAGCAGTACCGTCATCACCTTTATGACCAGTACAGC TTACACCAATAACCAGGCAGACATCGCCACCCAGGGCTGGTTCATCGGGCTCATGTGTGCCATTGCCCTT CTGGTGCTGATCCTTCTCATCGTCTGCTTCATCAAGAGGAGTCGAGGTGGCAAGTACCCAGTGCGGGAAA AGAAGGATGTCCCCTTGGGTCCTGAAGACCCCAAAGAAGAAGATGGCTCATTTGACTACAGTGATGAGGA CAACAAGCCCCTGCAGGGCAGCCAGACATCTCTGGATGGCACCATCAAGCAGCAGGAGAGCGATGACAGC CTGGTGGACTATGGCGAAGGCGGCGAGGGCCAGTTCAATGAAGATGGCTCCTTTATTGGCCAGTACACTG TCAAAAAGGACAAGGAGGAAACGGAGGGCAATGAGAGCTCAGAGGCCACATCACCAGTCAATGCCATCTA TTCCCTTGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR220931 representing NM_182716
Red=Cloning site Green=Tags(s) MARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPA PSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSP LWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTD YSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAW YKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAP GEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLAN AFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKM IRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKD DEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDR PRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQF RVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRR ETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDLPSAPRRFRVRQPNLET INLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGE AATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTT TTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTK KTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIAL LVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDS LVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_182716 |
| ORF Size | 3720 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_182716.4, NP_874385.1 |
| RefSeq Size | 9795 bp |
| RefSeq ORF | 3723 bp |
| Locus ID | 269116 |
| UniProt ID | Q810U3 |
| MW | 138.4 kDa |
| Gene Summary | This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined. [provided by RefSeq, May 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224043 | Nfasc (untagged) - Mouse neurofascin (Nfasc), transcript variant 1, (10ug) |
CNY 15,870.00 |
|
| MG220931 | Nfasc (tGFP-tagged) - Mouse neurofascin (Nfasc) transcript variant 1, (10ug) |
CNY 11,880.00 |
|
| MR220931L3 | Lenti ORF clone of Nfasc (Myc-DDK-tagged) - Mouse neurofascin (Nfasc), transcript variant 1 |
CNY 12,730.00 |
|
| MR220931L4 | Lenti ORF clone of Nfasc (mGFP-tagged) - Mouse neurofascin (Nfasc), transcript variant 1 |
CNY 12,730.00 |
