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Nfasc (NM_182716) Mouse Untagged Clone
CAT＃: MC224043
Nfasc (untagged) - Mouse neurofascin (Nfasc), transcript variant 1, (10ug)
CNY 15,870.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AA387016; D430023G06Rik; mKIAA0756; NF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224043 representing NM_182716
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGGCAGCAGGCGCCACCCTGGGTCCACATAGCCCTCATCCTCTTCCTCCTCAGCCTCGGAGGGG CCATCGAGATTCCGATGGACCCAAGCATTCAGAATGAGCTGACCCAACCCCCAACTATCACCAAGCAGTC CGTGAAGGACCACATCGTGGACCCTCGAGATAACATCCTGATTGAATGTGAAGCTAAAGGCAACCCCGCC CCCAGTTTTCACTGGACTCGCAACAGCAGATTCTTCAACATTGCCAAGGACCCACGGGTGTCCATGAGGA GGAGATCTGGGACCTTGGTGATCGACTTCCGCAGTGGTGGGCGGCCTGAGGAATACGAAGGGGAGTACCA GTGCTTTGCCCGGAACAAATTTGGCACTGCACTTAGCAACCGCATCCGCCTGCAGGTGTCCAAATCTCCC CTGTGGCCCAAGGAAAACCTAGACCCCGTCGTGGTTCAAGAGGGTGCCCCCTTGACACTACAGTGCAACC CCCCACCCGGCCTCCCGTCCCCCGTCATCTTCTGGATGAGCAGCTCCATGGAGCCCATCACCCAGGACAA GCGTGTCTCCCAGGGTCACAACGGGGACCTGTACTTCTCCAACGTCATGCTGCAGGACATGCAGACCGAC TACAGCTGCAACGCGCGCTTTCACTTCACCCACACCATTCAGCAGAAGAACCCCTTCACCCTCAAGGTCC TCACCACCCGAGGAGTTGCAGAAAGAACGCCCAGCTTCATGTATCCCCAGGGCACATCGAGCAGTCAGAT GGTTCTCCGTGGCATGGACCTGCTGCTGGAATGCATTGCCTCTGGCGTCCCAACACCAGACATTGCATGG TACAAGAAAGGTGGGGACCTCCCATCTAACAAGGCCAAGTTCGAGAACTTTAATAAGGCTCTGCGCATCA CCAATGTCTCTGAAGAGGACTCTGGGGAGTATTTCTGCCTGGCCTCCAACAAGATGGGCAGCATCCGGCA CACGATCTCGGTGAGAGTAAAGGCTGCTCCATACTGGCTGGATGAGCCCAAGAACCTGATCCTGGCTCCT GGGGAAGATGGGAGGCTGGTATGCCGAGCCAATGGGAACCCGAAGCCGACCGTGCAGTGGATGGTGAATG GAGAGCCTTTACAATCGGCACCACCCAATCCCAACCGTGAGGTAGCTGGAGACACTATCATCTTCCGGGA TACTCAGATCAGCAGCAGGGCAGTGTACCAATGTAATACATCCAATGAACATGGCTACCTGCTGGCCAAT GCCTTCGTCAGCGTGTTAGATGTACCCCCTCGGATGCTGTCTGCCCGCAACCAGCTCATCAGGGTGATCC TTTATAACCGGACACGGCTGGACTGTCCGTTCTTTGGGTCTCCCATCCCAACACTCCGATGGTTTAAGAA TGGGCAAGGAAGCAACCTGGATGGCGGTAACTACCACGTCTACGAAAACGGCAGTCTAGAAATCAAGATG ATTCGCAAAGAGGACCAAGGCATCTACACCTGTGTGGCCACCAACATCCTGGGCAAAGCCGAAAATCAAG TCCGCCTGGAGGTCAAAGACCCCACCAGGATCTACAGGATGCCCGAGGACCAGGTGGCCAAGAGGGGCAC CACGGTGCAGCTGGAGTGCCGCGTGAAACATGACCCCTCCTTGAAGCTCACAGTCTCCTGGCTGAAGGAC GATGAGCCACTCTACATTGGAAACAGGATGAAGAAGGAAGATGACTCCCTGACGATCTTCGGAGTGGCAG AGCGGGACCAGGGCAGTTACACGTGTATGGCCAGCACCGAGCTGGACCAGGACCTGGCAAAGGCCTACCT CACTGTTCTAGCTGATCAGGCCACTCCAACTAACCGTTTGGCTGCCCTACCCAAAGGGCGACCAGACCGA CCCAGGGACCTGGAGCTCACTGACCTGGCTGAGAGGAGTGTGAGGCTGACCTGGATCCCAGGGGATGACA ACAACAGCCCTATCACAGACTACGTCGTTCAGTTTGAAGAGGACCAGTTCCAACCAGGGGTGTGGCATGA CCACTCCAGGTTCCCAGGCAGCGTCAACTCAGCCGTCCTCCATCTGTCCCCATATGTCAACTACCAATTC AGAGTCATCGCTGTCAACGAGGTTGGGAGCAGCCACCCCAGCCTTCCATCCGAGCGGTACCGAACCAGTG GGGCACCCCCTGAATCTAATCCCAGTGATGTGAAGGGCGAAGGGACAAGAAAGAACAATATGGAGATCAC GTGGACGCCTATGAATGCTACCTCTGCCTTTGGCCCCAACCTACGCTACATTGTCAAGTGGCGACGGAGA GAGACCCGAGAGACTTGGAACAATGTCACAGTGTGGGGCTCTCGCTACGTGGTGGGGCAGACGCCTGTCT ACGTTCCCTATGAGATCCGAGTCCAGGCTGAAAATGACTTTGGGAAAGGCCCCGAGCCTGACACCATCAT TGGGTACTCCGGAGAAGATTTACCCAGTGCCCCCAGGCGGTTCAGAGTCCGACAGCCCAACCTGGAGACC ATCAACCTGGAGTGGGACCACCCAGAGCACCCCAACGGAATCCTGATTGGATACATCCTCAGATACGTGC CCTTTAATGGAACCAAACTGGGAAAGCAGATGGTGGAAAACTTCTCTCCCAATCAGACCAAGTTCTCTGT GCAGAGAGCAGACCCAGTGTCGCGTTACCGCTTCTCCCTCAGTGCCAGGACACAGGTGGGCTCTGGAGAA GCAGCCACAGAGGAGTCCCCAGCACCTCCAAATGAAGCTACTCCAACTGCAGCTCCTCCCACGTTGCCCC CGACTACTGTGGGTACCACAGGCCTTGTGAGCAGTACTGATGCTACTGCCCTTGCTGCCACCAGTGAAGC CACAACAGTTCCCATCATTCCAACCGTCGTACCTACCACCGTCGCCACCACCATTGCCACAACTACTACA ACCACTGCCGCCACCACCACCACCACCACTACGGAGAGCCCTCCCACTACCACTGCTGGGACTAAGATTC ACGAAACCGCCCCCGACGAGCAGTCCATTTGGAACGTCACAGTGCTCCCCAACAGTAAATGGGCCAACAT CACCTGGAAGCACAATTTCAGGCCTGGAACTGACTTTGTGGTTGAGTACATCGACAGCAACCATACGAAA AAAACTGTCCCTGTTAAGGCCCAGGCCCAGCCTATACAGCTGACAGACCTCTTTCCCGGGATGACGTACA CGTTGCGGGTGTATTCCCGGGACAACGAGGGCATCAGCAGTACCGTCATCACCTTTATGACCAGTACAGC TTACACCAATAACCAGGCAGACATCGCCACCCAGGGCTGGTTCATCGGGCTCATGTGTGCCATTGCCCTT CTGGTGCTGATCCTTCTCATCGTCTGCTTCATCAAGAGGAGTCGAGGTGGCAAGTACCCAGTGCGGGAAA AGAAGGATGTCCCCTTGGGTCCTGAAGACCCCAAAGAAGAAGATGGCTCATTTGACTACAGTGATGAGGA CAACAAGCCCCTGCAGGGCAGCCAGACATCTCTGGATGGCACCATCAAGCAGCAGGAGAGCGATGACAGC CTGGTGGACTATGGCGAAGGCGGCGAGGGCCAGTTCAATGAAGATGGCTCCTTTATTGGCCAGTACACTG TCAAAAAGGACAAGGAGGAAACGGAGGGCAATGAGAGCTCAGAGGCCACATCACCAGTCAATGCCATCTA TTCCCTTGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_182716 |
| Insert Size | 3723 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_182716.4, NP_874385.1 |
| RefSeq Size | 9795 bp |
| RefSeq ORF | 3723 bp |
| Locus ID | 269116 |
| UniProt ID | Q810U3 |
| Gene Summary | This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined. [provided by RefSeq, May 2009] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). This isoform lacks the third fibronectin type 3 (FNIII) repeat and includes the mucin-like domain. Isoform 1 is also known as NF186, as described by Davis et al. (PMID: 8947556). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG220931 | Nfasc (tGFP-tagged) - Mouse neurofascin (Nfasc) transcript variant 1, (10ug) |
CNY 11,880.00 |
|
| MR220931 | Nfasc (Myc-DDK-tagged) - Mouse neurofascin (Nfasc), transcript variant 1 |
CNY 8,632.00 |
|
| MR220931L3 | Lenti ORF clone of Nfasc (Myc-DDK-tagged) - Mouse neurofascin (Nfasc), transcript variant 1 |
CNY 12,730.00 |
|
| MR220931L4 | Lenti ORF clone of Nfasc (mGFP-tagged) - Mouse neurofascin (Nfasc), transcript variant 1 |
CNY 12,730.00 |
