Trit1 (NM_025873) Mouse Tagged ORF Clone
CAT#: MR220110
- TrueORF®
Trit1 (Myc-DDK-tagged) - Mouse tRNA isopentenyltransferase 1 (Trit1)
ORF Plasmid: tGFP
"NM_025873" in other vectors (4)
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CNY 4,660.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2310075G14Rik; AI314189; AI314635; AV099619; IPT; MOD5 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR220110 representing NM_025873
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCTGCGGCTGCTGCACGAGCAGTTCCTGTGAGCTCTGGATTCCGAGGCCTGCGGCGAACACTGC CACTTGTAGTGATTCTCGGGGCTACTGGGACTGGCAAGTCCACCCTGGCTCTGCAGTTAGGCCAGCGGCT CGGCGGCGAGATCGTCAGCGCCGACTCCATGCAGGTCTATGAAGGGCTAGACATCATCACCAATAAGGTC TCTGCCCAAGAGCAGAAGATGTGCCAGCACCACATGATCAGCTTTGTGGATCCACTTGTGACCAGCTACA CCGTGGTGGACTTTAGGAACAAAGCAACGGCCCTGATTGAAGATATTTTTGCTCGAGACAAAATTCCCAT TGTGGTGGGAGGAACCAATTATTACATTGAATCTCTGCTCTGGAAAGTTCTTATTACTACCAAGCCCCAG GAGATGGGCACTGGGAAAGTGGTTGATCGGAAAGTAGAGCTTGAAAAGGAAGATGGTCATGAACTCCATA AACGGCTAAGCCAGGTGGACCCAGAAATGGCCGCCAAGCTACACCCGCATGACAAACGCAAAGTGGCCAG GAGCTTGCAAGTGTTTGAAGAAACAGGAATCTCTCACAGTGAATTCCTTCATCGTCAGCACGCAGAGGAA GGCGGTGGTCCTCTTGGAGGCCCTCTCAGGTTCCCTAACCCATGCATCCTCTGGCTCCATGCTGACCAGG CAGTTCTAGATGAGCGCTTGGATAAAAGAGTGGATGACATGCTTGCTGCCGGACTCTTGGAAGAGCTGAG AGGTTTTCACAGACGTTATAATCTTAAGAACATTTCAGAAAATAGCCAGGACTATCAACACGGTATCTTC CAGTCAATTGGCTTCAAGGAATTTCACGAGTACCTGACCACTGAGGGGAAATGCACACCAGAGACTAGTA ACCAGCTTCTAAAGAAAGGTATTGAGGCTCTGAAACAAGTAACTAAGAGATATGCCCGGAAACAGAACCG ATGGGTTAAAAACCGCTTTTTGAGCAGACCTGGGCCCAGTGTCCCCCCGGTATATGGCTTAGAAGTATCT GATGTTTCCAAGTGGGAGGAGTCTGTTCTGGAACCTGCTCTCAATATAGTACAAAGTTTCATCCAGGGTC ACAAACCTACAGCCATGCCAGTGAAGATGGCATACAATGAAAGTGAGAACAAGAGAAGTTACCACATGTG TGACCTCTGTGACCGGATCATCATTGGGGACCGGGAATGGGCAGCACATTTAAAATCCAAATCTCACTTG CACCAATTGAAGAAAAGAAGAAGGTTGGACTTAGACGCTGTCAGTGCCACAGGAAGTCAAAGTAATTCCC CAGACTGTGACCCGGAACGCATCGAGGGGGAATCCTCGGGGCAGCACAATCAAGAGCTGAAGGCCAGTGT T ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR220110 representing NM_025873
Red=Cloning site Green=Tags(s) MAAAAAARAVPVSSGFRGLRRTLPLVVILGATGTGKSTLALQLGQRLGGEIVSADSMQVYEGLDIITNKV SAQEQKMCQHHMISFVDPLVTSYTVVDFRNKATALIEDIFARDKIPIVVGGTNYYIESLLWKVLITTKPQ EMGTGKVVDRKVELEKEDGHELHKRLSQVDPEMAAKLHPHDKRKVARSLQVFEETGISHSEFLHRQHAEE GGGPLGGPLRFPNPCILWLHADQAVLDERLDKRVDDMLAAGLLEELRGFHRRYNLKNISENSQDYQHGIF QSIGFKEFHEYLTTEGKCTPETSNQLLKKGIEALKQVTKRYARKQNRWVKNRFLSRPGPSVPPVYGLEVS DVSKWEESVLEPALNIVQSFIQGHKPTAMPVKMAYNESENKRSYHMCDLCDRIIIGDREWAAHLKSKSHL HQLKKRRRLDLDAVSATGSQSNSPDCDPERIEGESSGQHNQELKASV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_025873 |
ORF Size | 1401 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025873.3 |
RefSeq Size | 2059 bp |
RefSeq ORF | 1404 bp |
Locus ID | 66966 |
UniProt ID | Q80UN9 |
MW | 52.9 kDa |
Gene Summary | Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 of both cytosolic and mitochondrial tRNAs, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC216314 | Trit1 (untagged) - Mouse tRNA isopentenyltransferase 1 (Trit1), (10ug) |
CNY 6,080.00 |
|
MG220110 | Trit1 (tGFP-tagged) - Mouse tRNA isopentenyltransferase 1 (Trit1), (10ug) |
CNY 5,130.00 |
|
MR220110L3 | Lenti ORF clone of Trit1 (Myc-DDK-tagged) - Mouse tRNA isopentenyltransferase 1 (Trit1) |
CNY 6,560.00 |
|
MR220110L4 | Lenti ORF clone of Trit1 (mGFP-tagged) - Mouse tRNA isopentenyltransferase 1 (Trit1) |
CNY 6,560.00 |