Krt2 (NM_010668) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BB005427; Krt2-2; Krt2-17; Krt2e
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR218801 representing NM_010668, codon optimized. Due to the complexity of NM_010668, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTGCCAGATTTCTTGCAGAAGCAGGCGCGGCGGCGGCGGGGGGGGTGGCGGGGGGTTTCGGGGGT TCTCATCCGGATCAGCGGTAGTGTCTGGAGGCTCTCGGCGGTCCAATACTTCTTTTTCATGTATCAGTCG GCACGGTGGTGGTCGGGGAGGGTCAGGTGGAGGGGGATTTGGCTCTCAAAGCCTTGTGGGGCTTGGCGGC TACAAGTCTATTAGCTCTTCTGTGGCTGGCAATAGCGGCGGTTACGGAGGAAGCAGTTTTGGTGGGAGTT CCGGTTTTGGTGGGGGGCGCGGGTTCGGTGGTGGACAAGGTTTTGGTGGTTCAGGCGGGTTCGGAGGTGG CTCAGGCTTTGGCGGAGGGCAGGGCTTTGGCGGAGGCTCCCGGTTCGGGGGAGGTTCCGGCTTCGGGGGA GGAGGCTTCGGGGGAGGGTCCTTCGGAGGAGGACGGTTTGGTGGCGGTCCCGGTGGTTTCGGGGGACCCG GTGGGTTCCCTGGAGGCGGGATTCATGAAGTTAGCGTCAATCAGTCATTGTTGCAGCCCCTGGATGTGAA GGTCGATCCGGAAATTCAAAATGTAAAGAGCCAGGAGAGAGAACAGATCAAAACCCTGAATAATAAGTTC GCATCCTTCATAGACAAGGTACGGTTCCTCGAGCAGCAGAATCAAGTGCTGAGAACTAAATGGGAGCTGC TGCAACAACTGGATGTCGGCAGCCGCACTACCAACCTCGACCCCATCTTTCAGGCTTACATCGGCATGTT GAAGAAGCAAGTAGACCGGTTGTCCGCTGAGCGAACTAGCCAAGAGTCCGAACTTAACAATATGCAAGAC CTGGTGGAAGATTTCAAAAAGAAGTATGAGGATGAGATAAACAAAAGGACAAGCGCCGAAAACGACTTCG TTACCATTAAGAAAGATGTGGACTCATGCTATATGGATAAGACGGAATTGCAAGCACGCCTCGACATTCT GGCCCAGGAAGTTAACTTTCTCCGGACTTTGTACGATGCCGAGTTGTCCCAGCTCCAGCAAGATGTTACC GATACCAACGTGATCCTTTCTATGGACAACAACCGGAATCTGGATCTTGATTCTATTATCGCCGAGGTCC AGAATCAGTACGAAATGATCGCTCATAAGTCTAAGGCTGAGAGCGAGGAGCTTTACCATTCAAAATACGA GGAGCTCCAGGTAACAGCCGTCAAGCATGGAGACTCCCTCAAGGAGATCAAGATGGAGATCTCTGAGCTG AATCGCACGATCCAGAGGCTTCAGGGCGAGATTTCCCACGTTAAAAAGCAGTGTAAAGGGGTTCAGGATT CTATCGCCGACGCTGAGCAGCGGGGTGAACACGCCATTAAGGACGCCCGAGGCAAGTTGACCGATCTTGA GGAGGCTCTTCAGCAGTGCCGCGAAGATCTGGCCAGACTCTTGAGGGACTACCAGGAGCTGATGAATACT AAGCTGTCACTGGATGTGGAGATCGCAACATATCGCAAGTTGCTGGAAGGTGAGGAATGTAGAATGTCCG GGGACTTCTCAGATAATGTGAGCGTGTCTATCACTAGTTCCACGATTTCCTCTTCCGTGGCTTCCAAAAC CGGATTCGGGAGTGGAGGTCAGAGTAGCGGGGGTCGGGGGTCTTACGGCGGGCGCGGAGGAGGCGGCGGC GGAGGAAGCACCTACGGGTCAGGAGGCCGCAGTTCCGGATCAAGAGGTAGCGGGAGCGGCAGTGGGGGCG GGGGGTATTCCTCCGGAGGGGGCAGCAGAGGTGGCTCCGGGGGCGGATATGGTTCTGGTGGCGGTTCCCG CGGGGGCTCTGGGGGCGGGTACGGCAGCGGGGGCGGCAGTGGCAGCGGTGGTGGCTACAGCAGCGGAGGC GGCAGCCGGGGTGGTTCTGGCGGCGGGGGAGTTTCAAGCGGGGGAGGATCAAGGGGCGGCAGCTCCTCAG GGGGAGGCTCACGCGGCGGATCCTCTTCCGGCGGCGGCGGTTACAGTAGTGGCGGCGGAAGCCGGGGTGG TTCTAGCTCCGGCGGCGCCGGCAGTTCATCCGAAAAGGGTGGCAGCGGGTCCGGGGAGGGCTGCGGAAGC GGAGTGACATTCTCCTTTAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR218801 representing NM_010668 Red=Cloning site Green=Tags(s) MSCQISCRSRRGGGGGGGGGFRGFSSGSAVVSGGSRRSNTSFSCISRHGGGRGGSGGGGFGSQSLVGLGG YKSISSSVAGNSGGYGGSSFGGSSGFGGGRGFGGGQGFGGSGGFGGGSGFGGGQGFGGGSRFGGGSGFGG GGFGGGSFGGGRFGGGPGGFGGPGGFPGGGIHEVSVNQSLLQPLDVKVDPEIQNVKSQEREQIKTLNNKF ASFIDKVRFLEQQNQVLRTKWELLQQLDVGSRTTNLDPIFQAYIGMLKKQVDRLSAERTSQESELNNMQD LVEDFKKKYEDEINKRTSAENDFVTIKKDVDSCYMDKTELQARLDILAQEVNFLRTLYDAELSQLQQDVT DTNVILSMDNNRNLDLDSIIAEVQNQYEMIAHKSKAESEELYHSKYEELQVTAVKHGDSLKEIKMEISEL NRTIQRLQGEISHVKKQCKGVQDSIADAEQRGEHAIKDARGKLTDLEEALQQCREDLARLLRDYQELMNT KLSLDVEIATYRKLLEGEECRMSGDFSDNVSVSITSSTISSSVASKTGFGSGGQSSGGRGSYGGRGGGGG GGSTYGSGGRSSGSRGSGSGSGGGGYSSGGGSRGGSGGGYGSGGGSRGGSGGGYGSGGGSGSGGGYSSGG GSRGGSGGGGVSSGGGSRGGSSSGGGSRGGSSSGGGGYSSGGGSRGGSSSGGAGSSSEKGGSGSGEGCGS GVTFSFR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_010668
ORF Size	2121 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_010668.1, NM_010668.2, NP_034798.2
RefSeq Size	2629 bp
RefSeq ORF	2124 bp
Locus ID	16681
UniProt ID	Q3TTY5
MW	70.9 kDa
Gene Summary	Probably contributes to terminal cornification (By similarity). Associated with keratinocyte activation, proliferation and keratinization (By similarity). Plays a role in the establishment of the epidermal barrier on plantar skin (PubMed:26603179).[UniProtKB/Swiss-Prot Function]

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