Krt2 (NM_010668) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	BB005427; Krt2-2; Krt2-17; Krt2e
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC220732 representing NM_010668 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTGCCAGATCTCCTGCAGGTCCCGAAGAGGAGGAGGCGGCGGCGGAGGCGGCGGATTCAGGGGCT TCAGTAGCGGTTCAGCTGTGGTCTCTGGAGGGAGCCGGAGATCAAATACCAGCTTCTCCTGCATAAGCCG CCACGGTGGTGGCAGAGGAGGCTCTGGTGGAGGTGGCTTTGGTAGTCAGAGTCTTGTTGGCCTCGGCGGG TACAAGAGCATCTCTAGTAGCGTGGCTGGCAACAGTGGTGGCTACGGTGGCAGCAGCTTTGGAGGCAGCA GTGGCTTTGGAGGAGGCCGAGGATTTGGAGGTGGCCAAGGATTTGGAGGCAGCGGTGGCTTCGGAGGTGG CTCTGGCTTTGGAGGAGGCCAAGGATTTGGAGGTGGCAGCCGCTTTGGAGGTGGCAGCGGCTTCGGAGGT GGTGGATTTGGCGGAGGCAGTTTTGGCGGAGGCCGATTTGGAGGTGGCCCTGGAGGTTTTGGGGGCCCTG GAGGATTCCCTGGTGGAGGTATCCATGAAGTGTCTGTCAATCAGAGCCTCTTGCAGCCTCTTGATGTTAA AGTAGACCCAGAGATTCAGAACGTGAAGTCTCAGGAGCGGGAGCAGATCAAAACCCTCAACAACAAATTT GCCTCTTTCATTGACAAGGTGCGATTCCTGGAGCAGCAGAACCAGGTGTTGCGGACCAAATGGGAACTCC TGCAGCAGTTGGATGTAGGCAGCCGCACCACAAACCTGGACCCCATCTTCCAGGCCTACATAGGCATGCT CAAGAAGCAAGTGGACAGGCTTTCTGCAGAAAGGACCTCACAGGAGTCAGAGCTGAACAACATGCAGGAT CTCGTGGAAGACTTTAAGAAGAAGTACGAGGATGAGATCAACAAGCGCACGTCTGCGGAGAATGATTTTG TGACCATTAAAAAGGATGTGGACAGTTGCTACATGGACAAGACAGAGCTGCAGGCCAGGCTGGACATACT AGCACAGGAAGTCAACTTCCTGAGAACACTCTACGATGCGGAACTGTCCCAGCTACAGCAGGATGTCACC GACACCAATGTCATCCTGTCCATGGACAACAACCGGAACCTTGACCTGGACAGCATCATTGCTGAGGTTC AGAATCAATACGAGATGATCGCCCATAAGAGCAAGGCTGAGTCAGAGGAGCTGTACCACAGCAAGTATGA AGAACTCCAGGTCACTGCTGTGAAACACGGGGACAGCTTGAAAGAGATCAAGATGGAAATCAGCGAGTTG AACCGCACAATCCAGAGGCTGCAAGGGGAGATATCACATGTGAAGAAGCAGTGTAAGGGTGTACAAGACT CCATTGCAGATGCAGAGCAGCGTGGAGAGCATGCCATCAAAGATGCCAGGGGCAAGCTCACTGACCTGGA GGAGGCCCTACAACAGTGCCGAGAGGACCTGGCCCGGCTACTGAGGGACTACCAGGAGCTCATGAACACC AAGCTGTCCCTGGATGTGGAGATTGCCACCTACAGGAAGCTGCTGGAGGGGGAAGAGTGCAGGATGTCTG GAGACTTCAGCGACAACGTGTCTGTGTCCATCACAAGCAGCACCATCTCCTCATCTGTGGCATCTAAGAC TGGCTTCGGCTCTGGGGGTCAAAGTTCTGGAGGAAGAGGGTCTTATGGAGGACGAGGAGGAGGAGGAGGA GGAGGAAGCACCTATGGCTCTGGAGGCAGAAGCTCTGGCTCCAGAGGATCTGGATCCGGATCTGGAGGAG GAGGATACAGCTCTGGAGGAGGTTCCAGGGGAGGATCTGGAGGAGGATATGGCTCTGGAGGAGGCTCCAG GGGAGGATCTGGAGGAGGATACGGCTCTGGAGGTGGCTCCGGCTCTGGAGGAGGATACAGCTCTGGAGGA GGCTCCAGGGGAGGATCTGGAGGAGGAGGAGTCAGCTCTGGAGGAGGCTCTAGAGGAGGCTCCAGCTCTG GAGGAGGCTCCAGAGGTGGCTCCAGCTCTGGTGGGGGAGGATATAGTTCTGGAGGAGGCTCCAGAGGTGG CTCCAGCTCAGGAGGAGCAGGATCTAGCTCTGAAAAGGGAGGTTCTGGGTCAGGGGAAGGTTGTGGCTCA GGTGTGACCTTCTCCTTTAGATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_010668
Insert Size	2124 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_010668.2, NP_034798.2
RefSeq Size	2629 bp
RefSeq ORF	2124 bp
Locus ID	16681
UniProt ID	Q3TTY5
Gene Summary	Probably contributes to terminal cornification (By similarity). Associated with keratinocyte activation, proliferation and keratinization (By similarity). Plays a role in the establishment of the epidermal barrier on plantar skin (PubMed:26603179).[UniProtKB/Swiss-Prot Function]

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