United States
Germany
Japan
United Kingdom
China
Eya3 (NM_211357) Mouse Tagged Lenti ORF Clone
CAT＃: MR218788L3
- LentiORF®
Lenti ORF clone of Eya3 (Myc-DDK-tagged) - Mouse eyes absent 3 homolog (Drosophila) (Eya3), transcript variant 3
"NM_211357" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 6,080.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI844637 |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR218788).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_211357 |
| ORF Size | 1248 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_211357.1 |
| RefSeq Size | 4994 bp |
| RefSeq ORF | 1251 bp |
| Locus ID | 14050 |
| Gene Summary | Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1 (By similarity). Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. The phosphatase activity has been shown in vitro. Coactivates SIX1. Seems to coactivate SIX2, SIX4 and SIX5. The repression of precursor cell proliferation in myoblasts by SIX1 is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex and seems to be dependent on EYA3 phosphatase activity. May be involved in development of the eye. May play a role in mediating the induction and differentiation of cranial placodes.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG218788 | Eya3 (tGFP-tagged) - Mouse eyes absent 3 homolog (Drosophila) (Eya3) transcript variant 3, (10ug) |
CNY 4,560.00 |
|
| MR218788 | Eya3 (Myc-DDK-tagged) - Mouse eyes absent 3 homolog (Drosophila) (Eya3), transcript variant 3 |
CNY 4,180.00 |
|
| MR218788L4 | Lenti ORF clone of Eya3 (mGFP-tagged) - Mouse eyes absent 3 homolog (Drosophila) (Eya3), transcript variant 3 |
CNY 6,080.00 |
