United States
Germany
Japan
United Kingdom
China
Cfh (NM_009888) Mouse Tagged ORF Clone
CAT＃: MR218690
- TrueORF®
Cfh (Myc-DDK-tagged) - Mouse complement component factor h (Cfh)
ORF Plasmid: tGFP
"NM_009888" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 8,720.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Mud-1; NOM; Sas-1; Sas1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR218690 representing NM_009888
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTACAGCACAGATTTCTCTTGGAGTCAGTTGGTCCCAGAAAGATCCAAATTATGAGACTGTCAGCAA GAATTATTTGGCTTATATTATGGACTGTTTGTGCAGCAGAAGATTGTAAAGGTCCTCCTCCAAGAGAAAA TTCAGAAATTCTCTCAGGCTCGTGGTCAGAACAACTATATCCAGAAGGCACCCAGGCTACCTACAAATGC CGCCCTGGATACCGAACACTTGGCACTATTGTAAAAGTATGCAAGAATGGAAAATGGGTGGCGTCTAACC CATCCAGGATATGTCGGAAAAAGCCTTGTGGGCATCCCGGAGACACACCCTTTGGGTCCTTTAGGCTGGC AGTTGGATCTCAATTTGAGTTTGGTGCAAAGGTTGTTTATACCTGTGATGATGGGTATCAACTATTAGGT GAAATTGATTACCGTGAATGTGGTGCAGATGGGTGGATCAATGATATTCCACTATGTGAAGTTGTGAAGT GTCTACCTGTGACAGAACTCGAGAATGGAAGAATTGTGAGTGGTGCAGCAGAAACAGACCAGGAATACTA TTTTGGACAGGTGGTGCGGTTTGAATGCAATTCAGGCTTCAAGATTGAAGGACATAAGGAAATTCATTGC TCAGAAAATGGCCTTTGGAGCAATGAAAAGCCACGATGTGTGGAAATTCTCTGCACACCACCGCGAGTGG AAAATGGAGATGGTATAAATGTGAAACCAGTTTACAAGGAGAATGAAAGATACCACTATAAGTGTAAGCA TGGTTATGTGCCCAAAGAAAGAGGGGATGCCGTCTGCACAGGCTCTGGATGGAGTTCTCAGCCTTTCTGT GAAGAAAAGAGATGCTCACCTCCTTATATTCTAAATGGTATCTACACACCTCACAGGATTATACACAGAA GTGATGATGAAATCAGATATGAATGTAATTATGGCTTCTATCCTGTAACTGGATCAACTGTTTCAAAGTG TACACCCACTGGCTGGATCCCTGTTCCAAGATGTACCTTGAAACCATGTGAATTTCCACAATTCAAATAT GGACGTCTGTATTATGAAGAGAGCCTGAGACCCAACTTCCCAGTATCTATAGGAAATAAGTACAGCTATA AGTGTGACAACGGGTTTTCACCACCTTCTGGGTATTCCTGGGACTACCTTCGTTGCACAGCACAAGGGTG GGAGCCTGAAGTCCCATGCGTCAGGAAATGTGTTTTCCATTATGTGGAGAATGGAGACTCTGCATACTGG GAAAAAGTATATGTGCAGGGTCAGTCTTTAAAAGTCCAGTGTTACAATGGCTATAGTCTTCAAAATGGTC AAGACACAATGACATGTACAGAGAATGGCTGGTCCCCTCCTCCCAAATGCATCCGTATCAAGACATGTTC AGCATCAGATATACACATTGACAATGGATTTCTTTCTGAATCTTCTTCTATATATGCTCTAAATAGAGAA ACATCCTATAGATGTAAGCAGGGATATGTGACAAATACTGGAGAAATATCAGGATCAATAACTTGCCTTC AAAATGGATGGTCACCTCAACCCTCATGCATTAAGTCTTGTGATATGCCTGTATTTGAGAATTCTATAAC TAAGAATACTAGGACATGGTTTAAGCTCAATGACAAATTAGACTATGAATGTCTCGTTGGATTTGAAAAT GAATATAAACATACCAAAGGCTCTATAACATGTACTTATTATGGATGGTCTGATACACCCTCATGTTATG AAAGAGAATGCAGTGTTCCCACTCTAGACCGAAAACTAGTCGTTTCCCCCAGAAAAGAAAAATACAGAGT TGGAGATTTGTTGGAATTCTCCTGCCATTCAGGACACAGAGTTGGGCCAGATTCAGTGCAATGCTACCAC TTTGGATGGTCTCCTGGTTTCCCTACATGTAAAGGTCAAGTAGCATCATGTGCACCACCTCTTGAAATTC TTAATGGGGAAATTAATGGAGCAAAAAAAGTTGAATACAGCCATGGTGAAGTGGTGAAATATGATTGCAA ACCTAGATTCCTACTGAAGGGACCCAATAAAATCCAGTGTGTTGATGGGAATTGGACAACCTTGCCTGTA TGTATTGAGGAGGAGAGAACATGTGGAGACATTCCTGAACTTGAACATGGCTCTGCCAAGTGTTCTGTTC CTCCCTACCACCATGGAGATTCAGTGGAGTTCATTTGTGAAGAAAACTTCACAATGATTGGACATGGGTC AGTTTCTTGCATTAGTGGAAAATGGACCCAGCTTCCTAAATGTGTTGCAACAGACCAACTGGAGAAGTGT AGAGTGCTGAAGTCAACTGGCATAGAAGCAATAAAACCAAAATTGACTGAATTTACGCATAACTCCACCA TGGATTACAAATGTAGAGACAAGCAGGAGTACGAACGCTCAATCTGTATCAATGGAAAATGGGATCCTGA ACCAAACTGTACAAGCAAAACATCCTGCCCTCCTCCACCGCAGATTCCAAATACCCAAGTGATTGAAACC ACCGTGAAATACTTGGATGGAGAAAAATTATCTGTTCTTTGCCAAGACAATTACCTAACTCAGGACTCAG AAGAAATGGTGTGCAAAGATGGAAGGTGGCAGTCATTACCTCGCTGCATTGAAAAAATTCCATGTTCCCA GCCCCCTACAATAGAACATGGATCTATTAATTTACCCAGATCTTCAGAAGAAAGGAGAGATTCCATTGAG TCCAGCAGTCATGAACATGGAACTACATTCAGCTATGTCTGTGATGATGGTTTCAGGATACCTGAAGAAA ATAGGATAACCTGCTACATGGGAAAATGGAGCACTCCACCTCGCTGTGTTGGACTTCCTTGTGGACCTCC ACCTTCAATTCCTCTTGGTACTGTTTCTCTTGAGCTAGAGAGTTACCAACATGGGGAAGAGGTTACATAC CATTGTTCTACAGGCTTTGGAATTGATGGACCAGCATTTATTATATGCGAAGGAGGAAAGTGGTCTGACC CACCAAAATGCATAAAAACGGATTGTGACGTTTTACCCACAGTTAAAAATGCCATAATAAGAGGAAAGAG CAAAAAATCATATAGGACAGGAGAACAAGTGACATTCAGATGTCAATCTCCTTATCAAATGAATGGCTCA GACACTGTGACATGTGTTAATAGTCGGTGGATTGGACAGCCAGTATGCAAAGATAATTCCTGTGTGGATC CACCACATGTGCCAAATGCTACTATAGTAACAAGGACCAAGAATAAATATCTACATGGTGACAGAGTACG TTATGAATGTAATAAACCTTTGGAACTATTTGGGCAAGTGGAAGTGATGTGTGAAAATGGGATATGGACA GAAAAACCAAAGTGCCGAGACTCAACAGGGAAATGTGGGCCTCCTCCACCTATTGACAATGGAGACATCA CCTCCTTGTCATTACCAGTATATGAACCATTATCATCAGTTGAATATCAATGCCAGAAGTATTATCTCCT TAAGGGAAAGAAGACAATAACATGTAGAAATGGAAAGTGGTCTGAGCCACCAACATGCTTACATGCATGT GTAATACCAGAAAACATTATGGAATCACACAATATAATTCTCAAATGGAGACACACTGAAAAGATTTATT CCCATTCAGGGGAGGATATTGAATTTGGATGTAAATATGGATATTATAAAGCAAGAGATTCACCGCCATT TCGTACAAAGTGCATTAATGGCACCATCAATTATCCCACTTGTGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218690 representing NM_009888
Red=Cloning site Green=Tags(s) MVQHRFLLESVGPRKIQIMRLSARIIWLILWTVCAAEDCKGPPPRENSEILSGSWSEQLYPEGTQATYKC RPGYRTLGTIVKVCKNGKWVASNPSRICRKKPCGHPGDTPFGSFRLAVGSQFEFGAKVVYTCDDGYQLLG EIDYRECGADGWINDIPLCEVVKCLPVTELENGRIVSGAAETDQEYYFGQVVRFECNSGFKIEGHKEIHC SENGLWSNEKPRCVEILCTPPRVENGDGINVKPVYKENERYHYKCKHGYVPKERGDAVCTGSGWSSQPFC EEKRCSPPYILNGIYTPHRIIHRSDDEIRYECNYGFYPVTGSTVSKCTPTGWIPVPRCTLKPCEFPQFKY GRLYYEESLRPNFPVSIGNKYSYKCDNGFSPPSGYSWDYLRCTAQGWEPEVPCVRKCVFHYVENGDSAYW EKVYVQGQSLKVQCYNGYSLQNGQDTMTCTENGWSPPPKCIRIKTCSASDIHIDNGFLSESSSIYALNRE TSYRCKQGYVTNTGEISGSITCLQNGWSPQPSCIKSCDMPVFENSITKNTRTWFKLNDKLDYECLVGFEN EYKHTKGSITCTYYGWSDTPSCYERECSVPTLDRKLVVSPRKEKYRVGDLLEFSCHSGHRVGPDSVQCYH FGWSPGFPTCKGQVASCAPPLEILNGEINGAKKVEYSHGEVVKYDCKPRFLLKGPNKIQCVDGNWTTLPV CIEEERTCGDIPELEHGSAKCSVPPYHHGDSVEFICEENFTMIGHGSVSCISGKWTQLPKCVATDQLEKC RVLKSTGIEAIKPKLTEFTHNSTMDYKCRDKQEYERSICINGKWDPEPNCTSKTSCPPPPQIPNTQVIET TVKYLDGEKLSVLCQDNYLTQDSEEMVCKDGRWQSLPRCIEKIPCSQPPTIEHGSINLPRSSEERRDSIE SSSHEHGTTFSYVCDDGFRIPEENRITCYMGKWSTPPRCVGLPCGPPPSIPLGTVSLELESYQHGEEVTY HCSTGFGIDGPAFIICEGGKWSDPPKCIKTDCDVLPTVKNAIIRGKSKKSYRTGEQVTFRCQSPYQMNGS DTVTCVNSRWIGQPVCKDNSCVDPPHVPNATIVTRTKNKYLHGDRVRYECNKPLELFGQVEVMCENGIWT EKPKCRDSTGKCGPPPPIDNGDITSLSLPVYEPLSSVEYQCQKYYLLKGKKTITCRNGKWSEPPTCLHAC VIPENIMESHNIILKWRHTEKIYSHSGEDIEFGCKYGYYKARDSPPFRTKCINGTINYPTCV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009888 |
| ORF Size | 3756 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009888.3, NP_034018.2 |
| RefSeq Size | 4365 bp |
| RefSeq ORF | 3759 bp |
| Locus ID | 12628 |
| UniProt ID | P06909 |
| MW | 141.7 kDa |
| Gene Summary | Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces. Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop. As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b. In addition, mediates several cellular responses through interaction with specific receptors. For example, interacts with CR3/ITGAM receptor and thereby mediates the adhesion of human neutrophils to different pathogens. In turn, these pathogens are phagocytosed and destroyed.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224056 | Cfh (untagged) - Mouse complement component factor h (Cfh), (10ug) |
CNY 16,060.00 |
|
| MG218690 | Cfh (tGFP-tagged) - Mouse complement component factor h (Cfh), (10ug) |
CNY 12,070.00 |
|
| MR218690L3 | Lenti ORF clone of Cfh (Myc-DDK-tagged) - Mouse complement component factor h (Cfh) |
CNY 12,830.00 |
|
| MR218690L4 | Lenti ORF clone of Cfh (mGFP-tagged) - Mouse complement component factor h (Cfh) |
CNY 12,830.00 |
