United States
Germany
Japan
United Kingdom
China
Fpgs (NM_010236) Mouse Tagged ORF Clone
CAT＃: MR218569
- TrueORF®
Fpgs (Myc-DDK-tagged) - Mouse folylpolyglutamyl synthetase (Fpgs), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_010236" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 5,890.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA408187 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR218569 representing NM_010236
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTGGGCGCGGAGCCGACTGTGCTCGACTCTGTCCCTGGCAGCTGTTTCTGCGCGTGGTGCAACGA CGGAGGGCGCGGCGCGGCGGGGGATGAGCGCGTGGCCAGCGCCACAGGAGCCGGGCATGGAGTATCAGGA TGCTGTGCGCACGCTCAACACCCTGCAAACCAATGCCAGCTACCTGGAGCAGGTAAAGCGCCAACGGAGT GACCCCCAGGCGCAGCTGGAGGCTATGGAGATGTACCTGGCACGGAGTGGACTGCAGGTGGAGGACTTGA ACCGGCTAAACATTATTCATGTCACTGGGACCAAAGGGAAGGGCTCCACCTGTGCCTTCACCGAACGGAT CCTGCGGAATTACGGCCTGAAGACCGGCTTCTTTAGCTCTCCTCACATGGTGCAGGTGCGGGAGCGGATT CGAATCAACGGGAAACCAATCAGCCCCGAGCTCTTCACCAAGCACTTCTGGTGCCTCTATAACCAGCTGG AGGAGTTCAAGGACGACAGCCATGTCTCCATGCCCTCTTACTTCCGCTTCCTCACACTCATGGCCTTCCA TGTCTTCCTCCAAGAGAAGGTGGACCTGGCAGTGGTGGAGGTGGGCATTGGCGGGGCTTTTGACTGCACC AACATCATCAGAAAGCCAGTGGTGTGTGGAGTCTCCTCTCTTGGCATTGACCACACCAGTCTACTAGGAG ATACAGTGGAGAAAATAGCATGGCAGAAAGGGGGCATCTTTAAGCCTGGTGTCCCTGCCTTCACTGTGGT GCAGCCAGAAGGTCCCCTGGCTGTGCTGAGGGATCGAGCCCAGCAGATTGGATGCCCGTTGTACCTGTGT CCGCCATTGGAAGCCCTGGAGGAGGTTGGACTGCCATTGAGCCTGGGTCTGGAGGGAGCACACCAGCGGT CTAATGCTGCCTTGGCCTTGCAGCTGGCCCACTGTTGGCTGGAGCGGCAGGACCACCAAGACATCCAGGA GCTGAAGGTATCCAGGCCAAGCATACGGTGGCAGCTGCCCCTGGCACCTGTGTTCCGCCCTACCCCTCAC ATGAGGCGTGGGCTTCGGGACACAGTGTGGCCTGGCCGGACACAGATACTCCAGCGGGGACCCCTTACCT GGTACCTGGATGGCGCCCATACCACCAGCAGTGTGCAGGCCTGTGTGCACTGGTACCGCCAGTCATTGGA GCGCAGCAAACGCACCGATGGAGGGTCCGAAGTACACATCTTGCTCTTCAACTCTACTGGTGACAGGGAC TCTGCTGCCCTGCTGAAGCTGCTGCAGCCCTGCCAGTTTGACTACGCTGTCTTCTGCCCCAACGTGACAG AGGTTTCATCCATAGGAAATGCAGACCAGCAGAACTTCACTGTGACTCTGGACCAGGTGCTGCTCCGCTG CCTCCAACACCAGCAGCATTGGAACGGCCTGGCTGAGAAACAGGCCAGCTCCAACCTCTGGAGCAGCTGC GGCCCAGACCCTGCTGGGCCAGGCTCCCTGCTGCTGGCCCCGCACCCACCTCAGCCTACTAGGACGAGCT CCCTCGTTTTCAGCTGCATCTCCCACGCCTTGCTGTGGATCAGCCAAGGCCGGGATCCCATCTTTCAGCC CCAGAGCCTTCCAAGGAATCTTCTCAACCACCCCACAGCCAACAGCGGGGCCAGCATTCTCCGTGAGGCT GCTGCCATCCATGTACTGGTTACAGGAAGCCTGCACCTGGTGGGCGGGGTTCTGAAACTGCTGGATCCCT CTATGTCCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218569 representing NM_010236
Red=Cloning site Green=Tags(s) MSWARSRLCSTLSLAAVSARGATTEGAARRGMSAWPAPQEPGMEYQDAVRTLNTLQTNASYLEQVKRQRS DPQAQLEAMEMYLARSGLQVEDLNRLNIIHVTGTKGKGSTCAFTERILRNYGLKTGFFSSPHMVQVRERI RINGKPISPELFTKHFWCLYNQLEEFKDDSHVSMPSYFRFLTLMAFHVFLQEKVDLAVVEVGIGGAFDCT NIIRKPVVCGVSSLGIDHTSLLGDTVEKIAWQKGGIFKPGVPAFTVVQPEGPLAVLRDRAQQIGCPLYLC PPLEALEEVGLPLSLGLEGAHQRSNAALALQLAHCWLERQDHQDIQELKVSRPSIRWQLPLAPVFRPTPH MRRGLRDTVWPGRTQILQRGPLTWYLDGAHTTSSVQACVHWYRQSLERSKRTDGGSEVHILLFNSTGDRD SAALLKLLQPCQFDYAVFCPNVTEVSSIGNADQQNFTVTLDQVLLRCLQHQQHWNGLAEKQASSNLWSSC GPDPAGPGSLLLAPHPPQPTRTSSLVFSCISHALLWISQGRDPIFQPQSLPRNLLNHPTANSGASILREA AAIHVLVTGSLHLVGGVLKLLDPSMSQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010236 |
| ORF Size | 1761 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010236.2, NP_034366.2 |
| RefSeq Size | 2265 bp |
| RefSeq ORF | 1764 bp |
| Locus ID | 14287 |
| UniProt ID | P48760 |
| MW | 65.4 kDa |
| Gene Summary | Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis. Dihydrofolate, tetrahydrofolate, 5,10-methylenetetrahydrofolate, 10-formyltetrahydrofolate and 5-formyltetrahydrofolate are the best substrates. Folic acid and 5-methyltetrahydrofolate can also act as substrates.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG218569 | Fpgs (tGFP-tagged) - Mouse folylpolyglutamyl synthetase (Fpgs) nuclear gene encoding mitochondrial protein, (10ug) |
CNY 6,460.00 |
|
| MR218569L3 | Lenti ORF clone of Fpgs (Myc-DDK-tagged) - Mouse folylpolyglutamyl synthetase (Fpgs), nuclear gene encoding mitochondrial protein |
CNY 7,790.00 |
|
| MR218569L4 | Lenti ORF clone of Fpgs (mGFP-tagged) - Mouse folylpolyglutamyl synthetase (Fpgs), nuclear gene encoding mitochondrial protein |
CNY 7,790.00 |
