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Hsd3b7 (NM_133943) Mouse Tagged ORF Clone
CAT＃: MR218387
- TrueORF®
Hsd3b7 (Myc-DDK-tagged) - Mouse hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), transcript variant 1
ORF Plasmid: tGFP
"NM_133943" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI195443; BB098564 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR218387 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGACTCTGCACAGGTGCCAACACTGGTATACCTGGTCACAGGTGGCTGCGGCTTCCTGGGGGAAC ATATTGTTCGGATGCTGCTGGAACGGGAGCCCAGGCTCCGGGAGCTGCGTGTCTTTGACCTGCACCTGAG TTCTTGGCTGGAGGAGCTGAAAGCAGGGCCTGTGCAGGTGACTGCCATCCAGGGGGATGTGACTCAGGCC CATGAGGTGGCAGCAGCCATGTCTGGATCACATGTGGTCATCCATACAGCTGGGTTGGTGGATGTGTTTG GGAAGGCCAGTCCAAAGACCATCCACAAAGTCAACGTGCAGGGCACACAGAATGTGATTGATGCTTGTGT GCAGACTGGCACTCAGTACCTGGTCTACACGAGCAGCATGGAAGTGGTGGGGCCTAACATCAAGGGCCAC CCCTTCTACAGGGGCAATGAAGATACCCCATATGAGGCAGTCCACAGCCATCCCTACCCATGCAGTAAAG CCCTTGCTGAGCAGCTGGTCCTCGAGGCCAATGGAAGGAAGGTCAATGGAGGGCTACCCCTGGTGACATG TGCCCTTCGACCCACGGGCATTTATGGTGAAGGTCATCAGGTCATGAGAGACTTCTACTACCAGGGACTG CGCTTTGGAGGTCGTCTATTTCGGGCCGTCCCAGCTTCTGTGGAGCACGGTCGGGTCTATGTTGGCAATG TTGCTTGGATGCACATACTGGTGGCCCGGGAGCTGGAGCAGCGGGCAGCACTCATGGGTGGCCAGGTGTA TTTCTGCTATGATAAGTCACCTTATAAAAGCTACGAGGACTTCAACATGGAGTTTCTGAGTCCCTGTGGT CTTCGACTGATAGGCGCCCACCCACTGCTGCCCTACTGGCTGCTAGTGCTGCTGGCTACCCTCAATGCCC TGCTGCAGTGGCTGCTCCGCCCACTGGTGCTGTACACACCCCTGCTGAATCCCTACACGCTGGCTATGGC CAACACCACCTTTACTGTCAGTACCAACAAGGCACAGCGGCATTTTGGCTACAAGCCCCTCTTCTCATGG GAAGAGAGCAGGACCCGCACCATTCAGTGGGTGCAGGCGATGGAGGGTTCAGCTCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218387 protein sequence
Red=Cloning site Green=Tags(s) MADSAQVPTLVYLVTGGCGFLGEHIVRMLLEREPRLRELRVFDLHLSSWLEELKAGPVQVTAIQGDVTQA HEVAAAMSGSHVVIHTAGLVDVFGKASPKTIHKVNVQGTQNVIDACVQTGTQYLVYTSSMEVVGPNIKGH PFYRGNEDTPYEAVHSHPYPCSKALAEQLVLEANGRKVNGGLPLVTCALRPTGIYGEGHQVMRDFYYQGL RFGGRLFRAVPASVEHGRVYVGNVAWMHILVARELEQRAALMGGQVYFCYDKSPYKSYEDFNMEFLSPCG LRLIGAHPLLPYWLLVLLATLNALLQWLLRPLVLYTPLLNPYTLAMANTTFTVSTNKAQRHFGYKPLFSW EESRTRTIQWVQAMEGSAR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133943 |
| ORF Size | 1110 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133943.2, NP_598704.2 |
| RefSeq Size | 1823 bp |
| RefSeq ORF | 1110 bp |
| Locus ID | 101502 |
| UniProt ID | Q9EQC1 |
| MW | 41.1 kDa |
| Gene Summary | The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. HSD VII is active against four 7-alpha-hydroxylated sterols. Does not metabolize several different C(19/21) steroids as substrates. Involved in bile acid synthesis (PubMed:11067870). Plays a key role in cell positioning and movement in lymphoid tissues by mediating degradation of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC): 7-alpha,25-OHC acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells (PubMed:22999953).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC212072 | Hsd3b7 (untagged) - Mouse hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
| MG218387 | Hsd3b7 (tGFP-tagged) - Mouse hydroxy-delta-5-steroid dehydrogenase 3 beta- and steroid delta-isomerase 7 (Hsd3b7) transcript variant 1, (10ug) |
CNY 3,710.00 |
|
| MR218387L3 | Lenti ORF clone of Hsd3b7 (Myc-DDK-tagged) - Mouse hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), transcript variant 1 |
CNY 5,230.00 |
|
| MR218387L4 | Lenti ORF clone of Hsd3b7 (mGFP-tagged) - Mouse hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), transcript variant 1 |
CNY 5,230.00 |
