Sptbn1 (NM_009260) Mouse Tagged ORF Clone
CAT#: MR217862
- TrueORF®
Sptbn1 (Myc-DDK-tagged) - Mouse spectrin beta 2 (Spnb2), transcript variant 2
ORF Plasmid: tGFP
"NM_009260" in other vectors (2)
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CNY 21,470.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 9930031C03Rik; AL033301; elf1; elf3; mKIAA4049; Spnb-2; Spnb2; SPTB2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR217862 representing NM_009260
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTGCAGAGGACATCCAGCATTTCAGGGCCGCTGTCGCCGGCCTACACCGGGCAGGTGCCTTACA ACTACAACCAACTGGAAGGAAGATTCAAACAGCTCCAAGATGAGCGTGAAGCTGTACAGAAGAAGACCTT CACCAAGTGGGTCAATTCCCACCTTGCGAGAGTGTCCTGCCGAATCACAGACCTGTACACGGACCTTCGA GATGGACGGATGCTCATCAAGCTACTGGAGGTCCTCTCTGGAGAGAGGCTGCCTAAACCCACTAAGGGAC GGATGCGGATCCACTGTCTGGAGAATGTCGACAAGGCTCTTCAATTCCTGAAAGAGCAGAGAGTCCATCT TGAGAACATGGGCTCCCATGACATTGTGGATGGAAACCACCGGCTGACCCTCGGCCTCATCTGGACAATT ATTCTGCGCTTCCAGATCCAGGATATTAGTGTGGAGACTGAAGATAACAAAGAGAAAAAGTCTGCTAAGG ATGCATTGCTGCTGTGGTGCCAGATGAAGACAGCTGGGTACCCCAATGTCAACATTCACAATTTCACCAC TAGCTGGAGGGATGGCATGGCCTTCAATGCACTGATACATAAACATCGGCCTGACCTGATAGATTTTGAT AAACTGAAGAAATCTAATGCACACTACAATCTGCAGAATGCATTTAACCTGGCAGAGCAGCACCTTGGCC TCACTAAACTGTTAGACCCTGAAGATATCAGTGTGGACCACCCTGATGAGAAGTCTATCATCACATACGT GGTGACTTACTACCACTACTTCTCCAAGATGAAGGCCTTGGCTGTCGAAGGAAAGCGCATTGGAAAGGTG CTTGATAATGCTATAGAAACAGAGAAAATGATTGAGAAGTACGAGTCACTTGCTTCTGACCTTCTGGAGT GGATTGAACAAACCATCATCATCCTAAACAACCGCAAATTTGCTAATTCACTGGTTGGGGTCCAGCAGCA GCTCCAGGCATTCAACACGTACCGCACAGTGGAGAAACCACCTAAGTTTACTGAGAAGGGGAATTTGGAG GTGCTCCTTTTCACAATTCAGAGCAAGATGCGAGCGAATAATCAGAAGGTCTACATGCCCCGCGAGGGGA AGCTCATCTCTGACATCAACAAGGCCTGGGAAAGACTGGAAAAAGCAGAACATGAGAGAGAACTGGCTCT GCGGAATGAGCTCATACGGCAGGAAAAACTGGAACAACTCGCCCGAAGATTTGATCGCAAGGCAGCTATG AGGGAGACATGGCTGAGTGAAAACCAGCGTCTTGTGTCTCAGGACAACTTTGGATTTGACCTTCCCGCTG TTGAGGCTGCTACCAAAAAACACGAGGCCATTGAGACAGACATCGCTGCATATGAAGAACGAGTTCAGGC CGTGGTGGCTGTGGCCAGGGAACTTGAAGCCGAGAACTACCATGACATCAAGCGCATCACAGCGAGGAAG GACAATGTCATCCGGCTCTGGGAATACTTGCTGGAACTGCTCAGGGCCAGGAGGCAGCGTCTTGAGATGA ACCTGGGATTGCAAAAGATATTCCAGGAAATGCTTTATATTATGGACTGGATGGATGAAATGAAGGTGCT ATTGCTGTCTCAAGACTATGGCAAACACTTACTTGGTGTTGAAGACCTGTTACAGAAGCATGCCCTGGTT GAAGCAGACATTGCAATCCAAGCAGAGCGTGTAAGAGGTGTGAATGCCTCTGCCCAGAAGTTTGCAACAG ATGGGGAAGGCTACAAGCCATGTGACCCCCAGGTAATTCGAGACCGTGTTGCCCACATGGAGTTCTGCTA TCAAGAGCTTTGTCAGCTGGCTGCCGAGCGTAGGGCTCGCCTGGAAGAGTCCCGTCGCCTCTGGAAGTTC TTCTGGGAGATGGCAGAAGAGGAAGGCTGGATACGAGAGAAGGAGAAGATCCTGTCCTCTGATGATTACG GGAAAGACTTGACCAGTGTCATGCGCCTGCTGAGCAAGCACCGGGCATTTGAGGATGAGATGAGTGGCCG TAGTGGCCATTTTGAGCAGGCCATTAAAGAAGGTGAAGACATGATTGCAGAGGAACACTTTGGATCGGAA AAGATCCGTGAGAGAATCATTTATATCCGGGAGCAGTGGGCCAACCTGGAACAGCTCTCAGCCATTAGGA AGAAGCGCCTAGAGGAAGCCTCATTACTGCACCAGTTCCAGGCTGATGCTGATGATATTGATGCTTGGAT GTTAGATATACTCAAGATTGTCTCCAGCAATGATGTGGGCCATGATGAGTACTCCACGCAGTCTCTGGTC AAGAAGCATAAAGATGTAGCAGAAGAGATCACCAACTACAGGCCCACTATTGACACACTGCATGAGCAAG CCAGTGCCCTTCCACAAGCACATGCAGAGTCTCCAGATGTGAAGGGCCGGCTGGCAGGAATTGAGGAGCG CTGCAAGGAGATGGCAGAGTTAACACGGCTAAGGAAGCAGGCTCTGCAGGACACCCTGGCCCTGTACAAG ATGTTCAGTGAGGCTGATGCCTGTGAGCTCTGGATTGACGAGAAGGAGCAGTGGCTCAACAACATGCAGA TCCCAGAGAAGCTGGAGGACCTGGAAGTCATCCAGCACAGATTTGAGAGCCTAGAACCAGAAATGAACAA CCAGGCTTCCCGGGTTGCTGTGGTGAACCAGATTGCACGGCAGCTGATGCACAATGGCCACCCCAGTGAA AAGGAAATCAGAGCTCAGCAAGACAAACTCAACACGAGGTGGAGTCAGTTCAGAGAACTGGTGGACAGGA AAAAGGATGCTCTTCTGTCTGCCCTGAGCATCCAGAACTACCACCTCGAGTGCAATGAAACCAAATCCTG GATCCGGGAGAAGACCAAGGTCATCGAGTCTACCCAAGACCTTGGCAATGACCTGGCAGGTGTCATGGCC CTGCAGCGCAAGCTGACTGGCATGGAACGAGACTTGGTAGCCATTGAGGCGAAGCTGAGTGACCTGCAGA AAGAAGCTGAGAAGCTGGAGTCCGAGCACCCTGACCAGGCTCAAGCTATCCTGTCTCGGCTGGCCGAGAT CAGTGATGTGTGGGAGGAAATGAAGACAACCCTGAAGAACCGAGAGGCCTCCCTGGGAGAGGCCAGCAAG CTGCAGCAGTTTCTGCGGGACTTGGACGACTTCCAGTCTTGGCTCTCCAGGACCCAGACTGCTATCGCCT CAGAGGACATGCCCAATACCCTCACTGAGGCAGAGAAGCTTCTCACACAGCACGAGAATATCAAAAATGA GATCGACAATTATGAGGAAGACTACCAGAAGATGCGGGACATGGGCGAGATGGTCACCCAGGGGCAGACT GATGCCCAGTATATGTTTCTGCGGCAGCGGCTGCAGGCCTTAGACACTGGCTGGAATGAGCTCCACAAAA TGTGGGAGAACAGGCAAAACCTCCTCTCCCAGTCCCATGCCTACCAGCAGTTCCTTAGGGACACCAAACA AGCTGAAGCTTTTCTTAATAACCAGGAGTATGTTTTGGCTCATACTGAAATGCCCACCACCCTGGAAGGA GCTGAAGCAGCCATTAAAAAGCAGGAGGACTTCATGACCACCATGGATGCCAACGAGGAGAAGATCAATG CTGTTGTGGAGACTGGCCGAAGACTGGTGAGCGATGGGAACATCAACTCCGACCGCATCCAGGAGAAGGT GGACTCTATTGACGACAGACACAGGAAGAATCGAGAAGCAGCCAGTGAACTTCTGATGAGGTTAAAGGAC AACCGTGATCTACAGAAGTTCCTGCAAGATTGTCAAGAGCTGTCCCTCTGGATCAATGAAAAGATGCTTA CAGCTCAAGACATGTCTTATGATGAAGCCAGAAATCTGCACAGTAAATGGTTAAAGCATCAAGCATTTAT GGCGGAACTTGCATCCAACAAAGAATGGCTTGACAAAATTGAGAAGGAAGGAATGCAGCTTATTTCAGAA AAGCCAGAAACAGAAGCTGTGGTAAAGGAAAAACTCACTGGTTTACATAAAATGTGGGAAGTCCTTGAAT CCACAACCCAGACCAAGGCCCAGCGGCTCTTTGATGCAAATAAGGCTGAGCTTTTCACACAAAGCTGCGC AGATCTTGACAAATGGCTACATGGCCTGGAGAGCCAGATTCAATCTGACGACTATGGCAAAGACCTTACC AGTGTCAATATTCTTCTGAAAAAGCAACAGATGCTGGAGAATCAGATGGAAGTTCGGAAGAAAGAGATCG AGGAACTGCAGAGCCAAGCCCAGGCGCTGAGTCAGGAGGGGAAGAGCACAGATGAGGTGGACAGCAAACG CCTTACTGTGCAGACCAAGTTCATGGAGCTTCTGGAGCCCTTGAGTGAGAGGAAGCATAACCTGTTAGCT TCCAAGGAGATCCATCAGTTCAACAGGGATGTGGAGGACGAAATCCTATGGGTTGGCGAGAGGATGCCTT TGGCAACTTCCACAGATCATGGCCATAACCTTCAAACTGTGCAGCTGTTAATAAAGAAAAACCAGACCCT CCAGAAAGAAATCCAGGGACACCAGCCTCGTATTGATGACATCTTTGAGAGGAGTCAAAACATCATCACA GATAGCAGCAGCCTCAATGCCGAGGCTATCAGGCAGAGGCTCGCTGACCTGAAGCAGCTGTGGGGGCTCC TCATTGAGGAAACTGAGAAACGCCATAGACGGCTGGAGGAGGCACACAAGGCGCAGCAGTACTACTTTGA TGCAGCTGAAGCCGAGGCATGGATGAGTGAACAGGAGTTGTACATGATGTCTGAGGAAAAGGCCAAGGAT GAGCAGAGTGCTGTCTCTATGTTGAAAAAGCACCAGATTTTAGAGCAAGCTGTTGAGGACTATGCAGAGA CAGTACACCAGCTCTCCAAGACTAGCCGGGCGCTGGTGGCTGACAGCCATCCCGAAAGTGAGCGTATTAG CATGCGGCAGTCAAAGGTCGACAAGCTGTATGCTGGCCTGAAGGACCTTGCTGAGGAGAGGAGAGGAAAA CTTGATGAGAGGCACAGGCTGTTCCAGCTCAACAGAGAGGTGGATGACCTGGAACAGTGGATCGCTGAGA GGGAAGTGGTCGCAGGCTCCCATGAGTTGGGACAGGACTATGAGCATGTCACGATGTTACAAGAACGGTT CCGAGAATTTGCTCGAGACACAGGAAACATTGGGCAGGAGCGTGTGGATACAGTTAATAACATGGCAGAT GAACTCATCAACTCTGGACATTCAGATGCTGCCACCATTGCTGAGTGGAAAGATGGTCTCAATGAAGCCT GGGCTGACCTCCTGGAGCTCATTGACACAAGAACACAGATTCTTGCTGCCTCATATGAACTTCATAAGTT TTACCATGATGCCAAGGAGATCTTTGGCCGAATCCAGGACAAACACAAGAAACTCCCTGAGGAGCTTGGA AGAGATCAAAACACTGTGGAAACTTTACAGAGAATGCACACCACCTTTGAGCACGACATCCAAGCTCTGG GCACTCAGGTGAGGCAGCTGCAGGAGGATGCAGCTCGCCTCCAGGCAGCCTATGCAGGGGACAAGGCTGA TGACATCCAGAAGCGTGAGAATGAGGTCCTGGAAGCCTGGAAGTCCCTGCTGGATGCTTGTGAGGGTCGC AGGGTGCGGCTGGTAGACACAGGAGACAAGTTCCGCTTCTTCAGCATGGTGCGTGACCTCATGCTCTGGA TGGAAGATGTCATCCGGCAGATCGAGGCCCAGGAGAAACCACGGGATGTGTCATCTGTTGAACTGTTAAT GAATAATCATCAAGGTATCAAAGCTGAAATTGATGCTCGTAATGACAGCTTTACAGCCTGCATTGAGCTT GGGAAATCCCTGCTGGCACGGAAACACTATGCTTCTGAGGAGATCAAGGAAAAGTTACTGCAGCTGACAG AGAAAAGAAAAGAAATGATTGACAAGTGGGAAGACCGGTGGGAGTGGTTAAGACTGATTTTGGAGGTCCA TCAGTTCTCAAGGGATGCCAGTGTGGCAGAGGCTTGGCTGCTTGGACAGGAACCATACCTATCCAGCCGT GAAATTGGCCAGAGTGTAGACGAAGTGGAGAAGCTTATTAAGCGCCATGAGGCGTTTGAAAAGTCTGCAG CGACCTGGGATGAGAGATTCTCTGCTCTGGAAAGGCTGACAACGTTGGAGCTACTGGAAGTGCGCAGACA GCAAGAGGAAGAAGAAAGAAAGAGGCGGCCACCTTCTCCGGACCCAAACACGAAGGTTTCAGAGGAGGCT GAGTCCCAGCAATGGGATACTTCAAAAGGAGACCAAGTTTCCCAGAATGGTTTGCCGGCTGAGCAGGGAT CTCCACGGGTTAGTTACCGCTCTCAAACGTACCAAAACTACAAAAACTTTAATAGCAGACGGACAGCCAG TGACCATTCATGGTCTGGAATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217862 representing NM_009260
Red=Cloning site Green=Tags(s) MELQRTSSISGPLSPAYTGQVPYNYNQLEGRFKQLQDEREAVQKKTFTKWVNSHLARVSCRITDLYTDLR DGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTI ILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFD KLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKV LDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLE VLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAM RETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARK DNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLLLSQDYGKHLLGVEDLLQKHALV EADIAIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKF FWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSE KIRERIIYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSNDVGHDEYSTQSLV KKHKDVAEEITNYRPTIDTLHEQASALPQAHAESPDVKGRLAGIEERCKEMAELTRLRKQALQDTLALYK MFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHNGHPSE KEIRAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMA LQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASK LQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQT DAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEG AEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNREAASELLMRLKD NRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISE KPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLT SVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLSERKHNLLA SKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIIT DSSSLNAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHKAQQYYFDAAEAEAWMSEQELYMMSEEKAKD EQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGK LDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNNMAD ELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELG RDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACEGR RVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTACIEL GKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSR EIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPDPNTKVSEEA ESQQWDTSKGDQVSQNGLPAEQGSPRVSYRSQTYQNYKNFNSRRTASDHSWSGM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_009260 |
ORF Size | 6462 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009260.2, NP_033286.2 |
RefSeq Size | 9049 bp |
RefSeq ORF | 6465 bp |
Locus ID | 20742 |
UniProt ID | Q62261 |
MW | 251.6 kDa |
Gene Summary | Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.[UniProtKB/Swiss-Prot Function] |
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SDS |