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Plpp5 (NM_028000) Mouse Tagged ORF Clone
CAT＃: MR217773
- TrueORF®
Ppapdc1b (Myc-DDK-tagged) - Mouse phosphatidic acid phosphatase type 2 domain containing 1B (Ppapdc1b)
ORF Plasmid: tGFP
"NM_028000" in other vectors (4)
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CNY 2,400.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1810019D05Rik; 2310022A04Rik; AW045471; Ppapdc1; Ppapdc1b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR217773 representing NM_028000
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGACGGCGGCCCTGGGAGCCGAGCTGGGCGTGCGGGTCCTGCTCTTTGTGGCTTTCCTGGTGACCG AGCTGCTCCCTCCCTTCCAGCGGCGGATCCAGCCCGAGGAGCTGTGGCTCTACAGGAACCCGTACGTGGA GGCGGAATACTTCCCCACCGGCCGCATGTTTGTCATTGCCTTTCTCACTCCACTGTCCCTGATCTTCCTC GCCAAGTTTCTAAGGAAAGCTGACGCTACCGACAGCAAGCAAGCCTGCCTCGCTGCCAGCCTTGCCCTAG CTCTGAATGGTGTCTTTACCAACATAATCAAACTGATAGTGGGCAGGCCCCGCCCAGACTTCTTCTACCG CTGCTTCCCTGATGGGCTGGCCCATTCTGATCTGACGTGCACGGGGGACGAGGATGTGGTGAACGAGGGC CGAAAGAGCTTCCCCAGTGGACATTCTTCCTTTGCATTTGCTGGTCTGGCCTTTGCTTCCTTCTACCTGG CAGGGAAGTTACACTGCTTCACGCCACAAGGCCGAGGGAAGTCCTGGAGGCTCTGTGCCTTTCTGTCACC TTTACTCTTTGCAGCCGTGATTGCACTGTCCCGCACGTGTGACTACAAGCACCACTGGCAAGATGTGCTA GTTGGCTCCATGATTGGAATGACATTTGCCTATGTCTGCTACAGGCAGTACTACCCCCCTCTGACTGACG TAGAGTGCCATAAACCATTTCAGGACAAGCACAAACTTCCGTCCTCACAGAAGCCCAGTGAGTTGCACCA TTTGGAAATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217773 representing NM_028000
Red=Cloning site Green=Tags(s) MGTAALGAELGVRVLLFVAFLVTELLPPFQRRIQPEELWLYRNPYVEAEYFPTGRMFVIAFLTPLSLIFL AKFLRKADATDSKQACLAASLALALNGVFTNIIKLIVGRPRPDFFYRCFPDGLAHSDLTCTGDEDVVNEG RKSFPSGHSSFAFAGLAFASFYLAGKLHCFTPQGRGKSWRLCAFLSPLLFAAVIALSRTCDYKHHWQDVL VGSMIGMTFAYVCYRQYYPPLTDVECHKPFQDKHKLPSSQKPSELHHLEI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_028000 |
| ORF Size | 783 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_028000.1, NP_082276.1 |
| RefSeq Size | 1486 bp |
| RefSeq ORF | 783 bp |
| Locus ID | 71910 |
| UniProt ID | Q3UMZ3 |
| MW | 29.2 kDa |
| Gene Summary | Displays magnesium-independent phosphatidate phosphatase activity in vitro. Catalyzes the conversion of phosphatidic acid to diacylglycerol (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC211247 | Ppapdc1b (untagged) - Mouse phosphatidic acid phosphatase type 2 domain containing 1B (Ppapdc1b), (10ug) |
CNY 3,990.00 |
|
| MG217773 | Ppapdc1b (tGFP-tagged) - Mouse phosphatidic acid phosphatase type 2 domain containing 1B (Ppapdc1b), (10ug) |
CNY 2,850.00 |
|
| MR217773L3 | Lenti ORF clone of Ppapdc1b (Myc-DDK-tagged) - Mouse phosphatidic acid phosphatase type 2 domain containing 1B (Ppapdc1b) |
CNY 4,750.00 |
|
| MR217773L4 | Lenti ORF clone of Ppapdc1b (mGFP-tagged) - Mouse phosphatidic acid phosphatase type 2 domain containing 1B (Ppapdc1b) |
CNY 4,750.00 |
