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Sestd1 (NM_175465) Mouse Tagged ORF Clone
CAT＃: MR217700
- TrueORF®
Sestd1 (Myc-DDK-tagged) - Mouse SEC14 and spectrin domains 1 (Sestd1)
ORF Plasmid: tGFP
"NM_175465" in other vectors (4)
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CNY 5,096.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1500031J16Rik; A930010O20; Solo/Trio8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR217700 representing NM_175465
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCCTCGGTGATATTGCCCATTCTGAAGAAGAAGCTGGCCTTCCTTTCAGGAGGAAAGGACAGAC GCAGTGGACTCATCTTGACAATTCCGCTGTGCCTTGAGCAGACAAGTATGGATGAGCTGAGTGTCACTTT GGATTATCTACTCAGCATTCCAAGTGAGAAATGTAAGGCCAGAGGATTCACTGTGATCGTGGATGGCAGG AAGTCTCAGTGGAATGTGGTGAAGACAGTTGTCTTAATGCTGCAGAATGTTGTTCCAGCCGAGGTATCCC TTGTTTGTGTAGTGAAGCCAGATGAATTCTGGGATAAGAAAGTAACACACTTTTGTTTTTGGAAAGAAAA GGACAGACTTGGCTTTGAGGTTATTTTAGTGTCCGCCAATAAGTTGACGCGTTACATAGAGCCATGCCAG TTGACAGAAGACTTTGGTGGGAGTCTCACCTATGACCACATGGACTGGCTGAATAAGAGGCTGGTCTTTG AGAAGTTTACAAAGGAGTCTACATCATTGCTAGATGAACTTGCTCTGATTAACAATGGAAGTGATAAGGG GAATGAGCAGGAGAAAGAAAGGTCTGTGGATTTAAACTTTCTTCCATCCGTTGATCCTGAGACGGTTCTT CAGACAGGGCATGAGTTGTTATCTGAATTACAGCAGCGTCGATTTAATGGCTCCGACGGAGGGGTCTCGT GGTCTCCTATGGATGACGAGCTGCTGGCACAGCCGCAGGTTATGAAGTTGCTGGACTCACTCCGGGAGCA GTACACGCGCTACCAGGAAGTCTGCAGGCAGCGCAGTAAGCGCACACAGCTGGAGGAGATCCAGCAGAAG GTGATGCAGGTTGTGAACTGGCTTGAAGGGCCTGGATCTGAGCAACTGAGAGCACAGTGGGGCATTGGTG ACTCGATCAGGGCCTCCCAGGCCCTGCAACAGAAGCATGAGGAGATCGAGAGCCAACACAGCGAGTGGTT CGCAGTATACGTGGAGCTTAACCAGCAGATTGCAGCACTCTTAAACGCTGGAGACGAGGAAGATCTTGTG GAGTTGAAGTCTCTACAGCAACAGCTTAGCGACGTTTGCTATCGACAGGCCAGTCAGCTAGAATTTAGGC AAAACCTCCTGCAGGCAGCTTTGGAATTTCATGGTGTGGCCCAAGATTTGTCTCAGCAGTTGGACGGCTT ATTAGGGATGTTGTGCGTAGATGTAGCACCAGCTGATGGAGCATCGATTCAGCAAACTTTAAAACTGCTT GAAGAGAAGCTGAAAAGTGTTGATGTAGGATTGCAAGGCTTGCGAGAAAAAGGTCAAGGTCTCCTGGATC AGATCTCCAACCAGGCTTCCTGGGCCTATGGCAAGGATGTAACTATTGAAAATAAAGAAAACGTGGACCA CATACAAGGAGTGATGGAAGACATGCAACTTAGGAAACAAAGATGTGAGGACATGGTAGATGTGCGGAGG CTGAAGATGCTTCAGATGGTGCAGTTGTTTAAGTGTGAAGAGGATGCTTCACAGGCAGTAGAATGGCTGA GTGAACTTCTGGATGCCCTGCTGAAGACCCATATCAGGCTGGGTGACGATGCTCAGGAAACAAAGGTTTT ACTGGAAAAACACAGAAAATTTGTCGATGTTGCCCAGAGTACTTACGACTATGGCAGACAGCTGCTGCAG GCCACAGTTGTACTGTGCCAGTCTCTGCGCTGCACTTCCCGGTCCTCAGGGGACACACTTCCTCGACTGA ACAGAGTGTGGAAGCAGTTTACAGTCGCATCTGAAGAGAGAGTGCACAGGTTGGAGATGGCTATTGCCTT TCACTCCAATGCCGAAAAGATTTTGCAAGACTGCCCAGAAGAGCCTGAAGCAATGAATGATGAGGAACAG TTTGAGGAAATTGAAGCAATTGGAAAATCACTTCTGGATAGACTCACTATCCCCGTGGTTTACCCCGACG GAACTGAACAATATTTTGGAAGTCCAAGTGACATGGCTTCTACTGCAGAGCACATCAGGGACAGAATGAA ACTGGTCAGTCTCAAAAGGCAGCAGCTGAGACATCCCGAATTGGTGACCACGGAGAGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR217700 representing NM_175465
Red=Cloning site Green=Tags(s) MEASVILPILKKKLAFLSGGKDRRSGLILTIPLCLEQTSMDELSVTLDYLLSIPSEKCKARGFTVIVDGR KSQWNVVKTVVLMLQNVVPAEVSLVCVVKPDEFWDKKVTHFCFWKEKDRLGFEVILVSANKLTRYIEPCQ LTEDFGGSLTYDHMDWLNKRLVFEKFTKESTSLLDELALINNGSDKGNEQEKERSVDLNFLPSVDPETVL QTGHELLSELQQRRFNGSDGGVSWSPMDDELLAQPQVMKLLDSLREQYTRYQEVCRQRSKRTQLEEIQQK VMQVVNWLEGPGSEQLRAQWGIGDSIRASQALQQKHEEIESQHSEWFAVYVELNQQIAALLNAGDEEDLV ELKSLQQQLSDVCYRQASQLEFRQNLLQAALEFHGVAQDLSQQLDGLLGMLCVDVAPADGASIQQTLKLL EEKLKSVDVGLQGLREKGQGLLDQISNQASWAYGKDVTIENKENVDHIQGVMEDMQLRKQRCEDMVDVRR LKMLQMVQLFKCEEDASQAVEWLSELLDALLKTHIRLGDDAQETKVLLEKHRKFVDVAQSTYDYGRQLLQ ATVVLCQSLRCTSRSSGDTLPRLNRVWKQFTVASEERVHRLEMAIAFHSNAEKILQDCPEEPEAMNDEEQ FEEIEAIGKSLLDRLTIPVVYPDGTEQYFGSPSDMASTAEHIRDRMKLVSLKRQQLRHPELVTTES SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175465 |
| ORF Size | 2088 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175465.6, NP_780674.1 |
| RefSeq Size | 8951 bp |
| RefSeq ORF | 2091 bp |
| Locus ID | 228071 |
| UniProt ID | Q80UK0 |
| MW | 79.4 kDa |
| Gene Summary | May act as the primary docking protein directing membrane turnover and assembly of the transient receptor potential channels TRPC4 and TRPC5. Binds phospholipids such as phosphatidylinositol monophosphates, phosphatidylinositol diphosphates (PIP2s) and phosphatidic acid, but not less polar lipids including phosphatidylcholine, phosphatidylserine, and phosphatidylinositol. The binding to PIP2s is calcium dependent. Might be involved in the plasma membrane localization of CTNNB1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220622 | Sestd1 (untagged) - Mouse SEC14 and spectrin domains 1 (Sestd1), (10ug) |
CNY 9,030.00 |
|
| MG217700 | Sestd1 (tGFP-tagged) - Mouse SEC14 and spectrin domains 1 (Sestd1), (10ug) |
CNY 6,370.00 |
|
| MR217700L3 | Lenti ORF clone of Sestd1 (Myc-DDK-tagged) - Mouse SEC14 and spectrin domains 1 (Sestd1) |
CNY 7,700.00 |
|
| MR217700L4 | Lenti ORF clone of Sestd1 (mGFP-tagged) - Mouse SEC14 and spectrin domains 1 (Sestd1) |
CNY 7,700.00 |
