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Endov (NM_001164636) Mouse Tagged ORF Clone
CAT＃: MR217510
- TrueORF®
Endov (Myc-DDK-tagged) - Mouse RIKEN cDNA A730011L01 gene (A730011L01Rik), transcript variant 1
ORF Plasmid: tGFP
"NM_001164636" in other vectors (4)
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CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A730011L01Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR217510 representing NM_001164636
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCACACGGCCGCTGAGCGGCCGCCAGAGGAAACCCTGTCGCTGTGGAAAGGGGAACAAGCCCGGC TGAAAGCGCGCGTGGTGGATCGGGACACGGAGGCCTGGCAGCGGGACCCCAGCTTCTCGGGCCTGCAGAA GGTCGGGGGCGTGGATGTGTCCTTCGTGAAAGGGGACAGTGTCCGGGCCTGCGCTTCCCTGGTAGTGCTG AGCTACCCCGAGCTCAAGGTGGTGTATGAGGACAGCCGCATGGTTGGCCTGAAGGCCCCCTATGTGTCAG GCTTCCTGGCCTTCCGAGAGGTCCCTTTCCTGGTGGAGTTGGTACAGCGGCTGCAAGAGAAGGAACCAGA TCTCATGCCCCAGGTCGTTCTTGTGGATGGAAACGGGGTGCTTCACCAACGAGGCTTCGGGGTGGCCTGC CACCTTGGTGTCCTTACAGAGCTGCCATGCATCGGGGTGGCCAAGAAGCTCCTGCAGGTGGATGGACTGG AGAACAATGCTCTGCACAAGGAGAAGATTGTGCTCCTGCAGGCCGGAGGAGACACATTTCCTCTGATAGG CAGCTCTGGGACTGTCCTGGGAATGGCCCTGAGGAGCCATGACCACAGCACCAAGCCCCTCTATGTCTCT GTGGGCCACAGAATAAGCCTGGAGGTCGCTGTGCGCCTCACCCACCACTGCTGTAGGTTCCGGATCCCAG AACCTATACGCCAGGCTGACATCCGCTCTCGAGAGTACATCCGAAGGACTCTAGGGCAGCTTGGGGTGGC TCCTGCACAGAGAAAGGACAGGAGCCAGAAAGAGCAGAGGCCAAATGCATGCCCCCAAGGAGGCCCAGGA GCACTTGCAGATCAAGGCAGGCCTCCTGAATGCGACGGCAGAGACTCCAGCTCAGACCGGAAAGCCCCCG AGCCAGGCTTCCAGGAGCAGAAGGACCAGCAGTTGGAGGGAACCGGGCATCAGGAAGACTCGGACCTCTG GCCTCCTTCTCCAGCCTGGGTACAGTCACCACCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217510 representing NM_001164636
Red=Cloning site Green=Tags(s) MAHTAAERPPEETLSLWKGEQARLKARVVDRDTEAWQRDPSFSGLQKVGGVDVSFVKGDSVRACASLVVL SYPELKVVYEDSRMVGLKAPYVSGFLAFREVPFLVELVQRLQEKEPDLMPQVVLVDGNGVLHQRGFGVAC HLGVLTELPCIGVAKKLLQVDGLENNALHKEKIVLLQAGGDTFPLIGSSGTVLGMALRSHDHSTKPLYVS VGHRISLEVAVRLTHHCCRFRIPEPIRQADIRSREYIRRTLGQLGVAPAQRKDRSQKEQRPNACPQGGPG ALADQGRPPECDGRDSSSDRKAPEPGFQEQKDQQLEGTGHQEDSDLWPPSPAWVQSPP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001164636 |
| ORF Size | 1014 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001164636.1, NP_001158108.1 |
| RefSeq Size | 5112 bp |
| RefSeq ORF | 1017 bp |
| Locus ID | 338371 |
| UniProt ID | Q8C9A2 |
| MW | 37.6 kDa |
| Gene Summary | Endoribonuclease that specifically cleaves inosine-containing RNAs: cleaves RNA at the second phosphodiester bond 3' to inosine. Has strong preference for single-stranded RNAs (ssRNAs) toward double-stranded RNAs (dsRNAs). Cleaves mRNAs and tRNAs containing inosine. Also able to cleave structure-specific dsRNA substrates containing the specific sites 5'-IIUI-3' and 5'-UIUU-3'. Inosine is present in a number of RNAs following editing; the function of inosine-specific endoribonuclease is still unclear: it could either play a regulatory role in edited RNAs, or be involved in antiviral response by removing the hyperedited long viral dsRNA genome that has undergone A-to-I editing. Binds branched DNA structures (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC214633 | Endov (untagged) - Mouse RIKEN cDNA A730011L01 gene (A730011L01Rik), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
| MG217510 | Endov (tGFP-tagged) - Mouse RIKEN cDNA A730011L01 gene (A730011L01Rik) transcript variant 1, (10ug) |
CNY 3,710.00 |
|
| MR217510L3 | Lenti ORF clone of Endov (Myc-DDK-tagged) - Mouse RIKEN cDNA A730011L01 gene (A730011L01Rik), transcript variant 1 |
CNY 5,230.00 |
|
| MR217510L4 | Lenti ORF clone of Endov (mGFP-tagged) - Mouse RIKEN cDNA A730011L01 gene (A730011L01Rik), transcript variant 1 |
CNY 5,230.00 |
