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Srgap2 (NM_001081011) Mouse Tagged ORF Clone
CAT＃: MR217088
- TrueORF®
Srgap2 (Myc-DDK-tagged) - Mouse SLIT-ROBO Rho GTPase activating protein 2 (Srgap2)
ORF Plasmid: tGFP
"NM_001081011" in other vectors (4)
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CNY 7,456.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 9930124L22Rik; AI448945; FBP2; Fnbp2; srGAP3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR217088 representing NM_001081011
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGTCTCCAGCCAAATTCAAAAAGGATAAGGAGATCATCGCAGAGTACGATACTCAGGTCAAAGAGA TTAGGGCTCAGCTCACAGAGCAGATGAAATGCCTAGATCAGCAATGTGAGCTCCGGGTGCAGCTGTTGCA GGACCTGCAGGACTTCTTCCGCAAGAAGGCTGAGATTGAAATGGACTACTCTCGAAACCTGGAGAAGCTA GCCGAGCGCTTTCTAGCCAAGACACGAAGCACCAAGGACCAGCAATTTAAGAAGGACCAGAATGTTCTTT CTCCAGTCAACTGCTGGAACCTCCTCTTAAACCAAGTGAAGCGGGAGAGCAGGGATCATACCACCCTGAG TGACATCTACCTGAATAATATAATTCCTCGATTTGTTCAAGTCAGCGAGGACTCAGGAAGACTCTTTAAA AAGAGTAAAGAAGTTGGCCAGCAACTCCAAGATGATTTGATGAAAGTCCTGAACGAGCTTTACTCGGTCA TGAAGACATATCACATGTACAATGCCGACAGCATCAGTGCTCAGAGCAAACTGAAGGAAGCAGAGAAACA AGAAGAGAAGCAAATTGGCAAATCAGTAAAGCAAGAGGACCGGCAGACCCCTCGCTCCCCTGACTCCACA GCCAATGTCCGCATTGAGGAGAAGCATGTCCGGAGGAGCTCAGTGAAGAAGATTGAGAAGATGAAGGAGA AGCGACAAGCCAAGTACACAGAAAATAAGCTGAAGGCCATTAAAGCCCGGAATGAGTATTTATTGGCTTT GGAGGCAACCAATGCATCTGTCTTCAAGTACTATATCCATGACCTGTCTGATATTATTGATCAATGTTGT GACCTAGGCTACCATGCTAGCCTGAACCGGGCTCTACGCACTTTCCTATCTGCTGAATTAAATCTGGAAC AGTCAAAACATGAAGGTCTGGATGCTATTGAAAATGCAGTAGAAAACCTAGATGCCACCAGTGACAAGCA ACGGCTCATGGAGATGTACAACAATGTTTTTTGCCCCCCTATGAAATTTGAATTCCAGCCCCACATGGGA GATATGGCCTCTCAACTCTGTGCCCAGCAGCCTGTCCAGAGTGAGCTGGTGCAGAGATGCCAACAGCTGC AGTCTCGCTTATCCACTTTGAAGATTGAGAATGAAGAGGTGAAAAAGACAATGGAGGCCACCCTGCAGAC CATTCAAGACATTGTGACTGTTGAGGATTTTGATGTATCTGACTGCTTCCAGTATAGCAATTCCATGGAG TCTGTCAAATCAACTGTCTCTGAAACATTCATGAGCAAGCCCAGCATTGCTAAGAGGAGAGCGAACCAGC AAGAGACGGAACAGTTTTATTTCACGAAAATGAAGGAGTACTTGGAGGGTAGGAACCTCATCACAAAGCT ACAAGCCAAGCATGACCTCCTGCAGAAAACCCTGGGAGAAAGTCAGCGGACAGACTGTAGTCTTGCCAGG CGAAGCTCAACTGTGAGGAAACAGGATTCCAGCCAAGCAATTCCTCTGGTGGTAGAAAGCTGCATCCGGT TTATTAGCAGACATGGCCTACAACATGAGGGAATTTTCCGGGTTTCTGGATCACAAGTAGAAGTGAACGA CATAAAAAACGCCTTTGAGAGAGGAGAGGATCCCCTGGCTGGGGACCAGAATGACCATGACATGGACTCT ATAGCTGGTGTCCTCAAGCTTTACTTCCGGGGCCTGGAACACCCGCTCTTCCCTAAAGACATCTTCCATG ACTTGATTGCCTGTGTCACAATGGACAACCTGCAAGAGAGAGCTGTGCATATCCGGAAAGTCCTTCTGGT CCTGCCCAAGCCCACTCTGATTATCATGAGATATCTCTTTGCCTTCCTCAATCACTTATCACAGTTCAGT GAAGAGAACATGATGGACCCCTACAACCTTGCCATCTGCTTCGGGCCCTCACTGATGTCAGTGCCAGAGG GCCACGACCAGGTGTCCTGTCAAGCCCACGTGAATGAGCTGATCAAAACCATCATCATCCAACATGAGAA CATTTTCCCAAACCCCAGGGAGCTGGAGGGTCCCATCTACAGCAGAGGAGGAAGCATGGAGGATTACTGT GACAGCACTCATGGAGAGACTACCTCTGCTGAAGACTCCACCCAGGACGTTACAGCGGAGCACCACACAA GCGATGATGAATGTGAGCCCATAGAAGCCATTGCCAAGTTTGACTACGTAGGCCGGACAGCCCGAGAACT GTCTTTCAAGAAGGGAGCATCCCTGCTGCTCTACCAGCGAGCTTCTGATGACTGGTGGGAGGGCCGGCAC AACGGTATAGATGGACTCATCCCCCATCAGTACATCGTAGTCCAAGACACCGAGGACGGTGTCGTGGAGA GGTCCAGCCCCAAGTCTGAGATTGAGGTCATGTCTGAGCCACCTGAAGAAAAGGTGACAGCCAGAACGGG GGCCAGCTGTCCCAGTGGGGGTCATGTAGCTGATATTTATCTTGCAAACATCAACAAGCAAAGGAAGCGT CCAGAATCTGGGAGCATCAGAAAAGCATTTCGGAGTGACAGCCATGGGCTGGGCAGTTCTCTGACTGACT CCTCCTCCCTGGGGGTGGGGGCTAGCTGCCGTCCATCCTCCCAGCCCATCATGAGCCAGAATCTCCCCAA GGAAGGGCCAGATAAGTGTTCCATCAGCGGCCATGGCAGCCTCAACTCTATCAGCCGCCACTCATCCTTG AAGAACCGGATGGACAGTCCGCAGATCCGGAAGACCGCTACGGCAGGAAGGTCAAAAAGTTTCAATAACC ATCGGCCCATGGACCCTGAAGTCATTGCACAGGATATTGAAGCAACAATGAACTCTGCCCTGAATGAGCT TCAAGAGCTAGAGCGGCAGAGCAGTGCTAAGCACACACCTGATGTGGTTCTGGACACCTTGGAACCACTC AAGACCTCCCCGGTGGTAGCCCCCACATCTGAGCCCTCCAGCCCTCTGCACACCCAGCTCCTCAAGGACC CTGAGCCTGCCTTCCAGCGCAGCGCTAGTACTGCTGGGGACATTGCCTGCGCCTTCCGGCCTGTAAAGTC TGTCAAGATGGCTGCTCCAGTCAAACCACCAGCCACACGGCCCAAGCCAACTGTCTTCCCCAAAACAAAC GCCACTAGCCCTGGTGTCAATTCATCTGCTTCCCCACAGGCCACTGACAAGTCTTGTACTGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217088 representing NM_001081011
Red=Cloning site Green=Tags(s) MTSPAKFKKDKEIIAEYDTQVKEIRAQLTEQMKCLDQQCELRVQLLQDLQDFFRKKAEIEMDYSRNLEKL AERFLAKTRSTKDQQFKKDQNVLSPVNCWNLLLNQVKRESRDHTTLSDIYLNNIIPRFVQVSEDSGRLFK KSKEVGQQLQDDLMKVLNELYSVMKTYHMYNADSISAQSKLKEAEKQEEKQIGKSVKQEDRQTPRSPDST ANVRIEEKHVRRSSVKKIEKMKEKRQAKYTENKLKAIKARNEYLLALEATNASVFKYYIHDLSDIIDQCC DLGYHASLNRALRTFLSAELNLEQSKHEGLDAIENAVENLDATSDKQRLMEMYNNVFCPPMKFEFQPHMG DMASQLCAQQPVQSELVQRCQQLQSRLSTLKIENEEVKKTMEATLQTIQDIVTVEDFDVSDCFQYSNSME SVKSTVSETFMSKPSIAKRRANQQETEQFYFTKMKEYLEGRNLITKLQAKHDLLQKTLGESQRTDCSLAR RSSTVRKQDSSQAIPLVVESCIRFISRHGLQHEGIFRVSGSQVEVNDIKNAFERGEDPLAGDQNDHDMDS IAGVLKLYFRGLEHPLFPKDIFHDLIACVTMDNLQERAVHIRKVLLVLPKPTLIIMRYLFAFLNHLSQFS EENMMDPYNLAICFGPSLMSVPEGHDQVSCQAHVNELIKTIIIQHENIFPNPRELEGPIYSRGGSMEDYC DSTHGETTSAEDSTQDVTAEHHTSDDECEPIEAIAKFDYVGRTARELSFKKGASLLLYQRASDDWWEGRH NGIDGLIPHQYIVVQDTEDGVVERSSPKSEIEVMSEPPEEKVTARTGASCPSGGHVADIYLANINKQRKR PESGSIRKAFRSDSHGLGSSLTDSSSLGVGASCRPSSQPIMSQNLPKEGPDKCSISGHGSLNSISRHSSL KNRMDSPQIRKTATAGRSKSFNNHRPMDPEVIAQDIEATMNSALNELQELERQSSAKHTPDVVLDTLEPL KTSPVVAPTSEPSSPLHTQLLKDPEPAFQRSASTAGDIACAFRPVKSVKMAAPVKPPATRPKPTVFPKTN ATSPGVNSSASPQATDKSCTV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001081011 |
| ORF Size | 3213 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001081011.2, NP_001074480.2 |
| RefSeq Size | 8040 bp |
| RefSeq ORF | 3216 bp |
| Locus ID | 14270 |
| UniProt ID | Q91Z67 |
| MW | 120.8 kDa |
| Gene Summary | RAC1 GTPase activating protein (GAP) that binds and deforms membranes, and regulates actin dynamics to regulate cell migration and differentiation. Plays an important role in different aspects of neuronal morphogenesis and migration mainly during development of the cerebral cortex. This includes the biogenesis of neurites, where it is required for both axons and dendrites outgrowth, and the maturation of the dendritic spines. Also stimulates the branching of the leading process and negatively regulates neuron radial migration in the cerebral cortex. May play a role for cognition, learning and memory. In non-neuronal cells, it may also play a role in cell migration by regulating the formation of lamellipodia and filopodia.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223445 | Srgap2 (untagged) - Mouse SLIT-ROBO Rho GTPase activating protein 2 (Srgap2), (10ug) |
CNY 13,780.00 |
|
| MG217088 | Srgap2 (tGFP-tagged) - Mouse SLIT-ROBO Rho GTPase activating protein 2 (Srgap2), (10ug) |
CNY 9,220.00 |
|
| MR217088L3 | Lenti ORF clone of Srgap2 (Myc-DDK-tagged) - Mouse SLIT-ROBO Rho GTPase activating protein 2 (Srgap2) |
CNY 10,260.00 |
|
| MR217088L4 | Lenti ORF clone of Srgap2 (mGFP-tagged) - Mouse SLIT-ROBO Rho GTPase activating protein 2 (Srgap2) |
CNY 10,260.00 |
