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Akap4 (NM_009651) Mouse Tagged ORF Clone
CAT＃: MR216759
- TrueORF®
Akap4 (Myc-DDK-tagged) - Mouse A kinase (PRKA) anchor protein 4 (Akap4), transcript variant 1
ORF Plasmid: tGFP
"NM_009651" in other vectors (4)
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CNY 6,216.00
CNY 7,410.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AKAP-4; AKAP82; Fsc1; mAKAP82; p82; PRKA4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR216759 representing NM_009651
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTGCCTACTGTGGTACTACAACGATGTCTGACGATATTGACTGGTTACACAGCCGCAGAGGCGTGT GCAAGGTAGATCTTTACAGTCCAAAAGGACAACAAGATCAGGACCGAAAAGTGATATGCTTTGTGGATGT GTCTACCTTGAATGTGGAAGATAAAGATTCCAAGGGTGCTGCTGGTTCCAGGTCAGAAGGCGAGTTAAAT CTGGAGACTCTGGAAGAAAAAGAGATTATCGTAATCAAGGACACTGAAAAACAAGACCAGTCTAAGACGG AGGGATCTGTGTGCCTTTTCAAACAAGCTCCCTCTGATCCTATAAGTGTCCTCAACTGGCTCCTCAATGA TCTCCAGAAGTATGCCTTGGGTTTCCAACATGCATTAAGCCCCTCAGCCTCTAGCTGTAAACATAAAGTG GGAGACCTAGAGGGTGACTATAGCAAAATACCATCTGAGAACTGCTACAGTGTGTACGCTGATCAAGTAA ACTTTGATTATTTGAACAAAGGACCTCAAAACCTTCGCCTAGAAATGGCGGCATCCAAAAACACCAACAA TAACCAAAGTCCCTCAAATCCTGCAACCAAATCTCCTAGCAATCAGAGGTCAGTTGCCACTCCTGAGGGA GAATGTTCTATGGACGACCTTTCCTTCTATGTCAACCGATTGTCATCTCTGGTAATCCAAATGGCCCGTA AGGAAATCAAGGACAAATTGGAAGGTGGAAGCAAATGTCTCCATCATTCCATGTATACATCAGGAGATAA AGGGAAAACCAGCCCCCGGAGTGCTGTCAGCAAAATTGCTTCGGAGATGGCCCATGAAGCTGTTGAACTG ACCTCATCAGAAATGCGTGGCAATGGAGAGGATTGCAGGGATGGCCGGAAAACCTTTTTGTATAGTGAAA TGTGTAACAAGAACAAGTGTGGCGAAAAGCAGCAGATGTGCCCAAAAGACAGCAAAGAATTTGCGGATTC CATCAGCAAGGGGCTTATGGTTTATGCAAATCAAGTAGCATCTGACATGATGGTCTCTGTTATGAAAACT TTGAAAGTACACAGCTGTGGGAAGCCAATTCCGGCTTGTGTGGTCCTGAAGAGGGTACTATTGAAGCACA CCAAGGAAATTGTATCTGATCTGATTGATTCATGTATGAAAAACTTGCATAACATAACAGGAGTCCTGAT GACAGACTCAGACTTCGTTTCTGCTGTCAAGAGGAATCTTTTCAATCATGGTAAACAAAATGCAGCGGAC ATCATGGAGGCCATGCTAAAGCGTCTGGTCAGTGCTCTTCTTGGTGAGAAGAAGGAGACTAAGTCTCAAA GTCTGGCCTATGCAACCTTAAAAGCTGGAACTAATGATCCGAAATGCAAGAATCAGAGCCTTGAGTTCTC AGCTATGAAAGCTGAAATGAAAGGAAAAGATAAATGCAAATCCAAAGCAGATCCATGCTGCAAATCGTTG ACAAGTGCTGAGAGAGTCAGCGAGCATATCCTCAAAGAGAGCCTTACTATGTGGAACAACCAGAAGCAAG GAAACCAAGGCAAAGTGACTAACAAAGTTTGCTGCACCAGTAAAGATGAGAAGAGAGAAAAGATCAGTCC TTCCACAGATTCACTGGCCAAGGATCTAATTGTCTCTGCCCTTATGCTCATTCAATACCATCTGACCCAA CAAGCCAAGGGCAAAGATCCATGTGAAGAGGAGTGCCCTGGTTCCTCCATGGGGTATATGTCCCAAAGTG CACAATACGAAAAATGTGGAGGTGGCCAAAGTTCTAAATCACTTTCAATGAAGCATTTTGAAACTCGTGG AGCTCCTGGACCATCTACATGTATGAAGGAAAATCAACTGGAGTCCCAGAAGATGGATATGTCAAACATG GTTCTGTCCCTGATTCAGAAACTCCTGAGTGAGAGCCCTTTCAGTTGCGATGAACTAACTGAAAGTGACA ATAAGCGTTGTTGTGATCCTAGATCAAGCAAAGCAGCTCCCATGGCCAAGAGACCTGAAGAGCAATGCCA AGACAATGCAGAACTAGACTTCATCAGTGGGATGAAGCAAATGAACCGCCAGTTTATAGATCAGCTGGTA GAATCCGTGATGAAACTCTGCCTGATCATGGCTAAGTACAGCAACAATGGAGCAGCCCTGGCTGAGCTGG AAGAACAAGCAGCCCTGGTAGGCAGTGGCTCCAGATGTGGCCGTGATGCTATGATGTCACAAAATTATTC TGAAACTCCTGGCCCTGAAGTTATTGTCAACAATCAGTGCTCTACAACTAACTTGCAGAAGCAGCTGCAG GCTGTCCTGCAATGGATTGCAGCCTCTCAATTCAATGTGCCCATGCTCTACTTCATGGGAGACGATGATG GACAACTAGAGAAGCTCCCTGAAGTTTCAGCTAAGGCTGCAGAGAAGGGGTACAGTGTAGGAGATCTTCT TCAGGAGGTCATGAAGTTTGCCAAAGAACGACAACTGGATGAAGCCGTGGGCAACATGGCTAGAAAGCAG CTGCTAGACTGGCTTCTCGCTAACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216759 representing NM_009651
Red=Cloning site Green=Tags(s) MIAYCGTTTMSDDIDWLHSRRGVCKVDLYSPKGQQDQDRKVICFVDVSTLNVEDKDSKGAAGSRSEGELN LETLEEKEIIVIKDTEKQDQSKTEGSVCLFKQAPSDPISVLNWLLNDLQKYALGFQHALSPSASSCKHKV GDLEGDYSKIPSENCYSVYADQVNFDYLNKGPQNLRLEMAASKNTNNNQSPSNPATKSPSNQRSVATPEG ECSMDDLSFYVNRLSSLVIQMARKEIKDKLEGGSKCLHHSMYTSGDKGKTSPRSAVSKIASEMAHEAVEL TSSEMRGNGEDCRDGRKTFLYSEMCNKNKCGEKQQMCPKDSKEFADSISKGLMVYANQVASDMMVSVMKT LKVHSCGKPIPACVVLKRVLLKHTKEIVSDLIDSCMKNLHNITGVLMTDSDFVSAVKRNLFNHGKQNAAD IMEAMLKRLVSALLGEKKETKSQSLAYATLKAGTNDPKCKNQSLEFSAMKAEMKGKDKCKSKADPCCKSL TSAERVSEHILKESLTMWNNQKQGNQGKVTNKVCCTSKDEKREKISPSTDSLAKDLIVSALMLIQYHLTQ QAKGKDPCEEECPGSSMGYMSQSAQYEKCGGGQSSKSLSMKHFETRGAPGPSTCMKENQLESQKMDMSNM VLSLIQKLLSESPFSCDELTESDNKRCCDPRSSKAAPMAKRPEEQCQDNAELDFISGMKQMNRQFIDQLV ESVMKLCLIMAKYSNNGAALAELEEQAALVGSGSRCGRDAMMSQNYSETPGPEVIVNNQCSTTNLQKQLQ AVLQWIAASQFNVPMLYFMGDDDGQLEKLPEVSAKAAEKGYSVGDLLQEVMKFAKERQLDEAVGNMARKQ LLDWLLANL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009651 |
| ORF Size | 2547 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009651.4, NP_033781.2 |
| RefSeq Size | 2855 bp |
| RefSeq ORF | 2550 bp |
| Locus ID | 11643 |
| UniProt ID | Q60662 |
| MW | 93.8 kDa |
| Gene Summary | Major structural component of sperm fibrous sheath. Plays a role in sperm motility (PubMed:12167408).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222038 | Akap4 (untagged) - Mouse A kinase (PRKA) anchor protein 4 (Akap4), transcript variant 1, (10ug) |
CNY 10,830.00 |
|
| MG216759 | Akap4 (tGFP-tagged) - Mouse A kinase (PRKA) anchor protein 4 (Akap4) transcript variant 1, (10ug) |
CNY 8,170.00 |
|
| MR216759L3 | Lenti ORF clone of Akap4 (Myc-DDK-tagged) - Mouse A kinase (PRKA) anchor protein 4 (Akap4), transcript variant 1 |
CNY 9,310.00 |
|
| MR216759L4 | Lenti ORF clone of Akap4 (mGFP-tagged) - Mouse A kinase (PRKA) anchor protein 4 (Akap4), transcript variant 1 |
CNY 9,310.00 |
