Shisa3 (NM_001033415) Mouse Tagged ORF Clone
CAT#: MR216748
- TrueORF®
Shisa3 (Myc-DDK-tagged) - Mouse shisa homolog 3 (Xenopus laevis) (Shisa3)
ORF Plasmid: tGFP
"NM_001033415" in other vectors (4)
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CNY 2,400.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | D830007B15Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR216748 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGCGCTGCTGGCGTTCTGTCTGCTCGTGGGCTTGCTACGTTGGGGCCCGGCGGGCGCGCAGCAGC CTGGGGAATACTGCCACGGCTGGGTGGACGCGCAGGGCAACTATCACGAGGGGTTCCAGTGCCCCGAGGA CTTCGACACGCAGGACGCCACCATCTGCTGCGGCTCGTGTGCGCTGCGCTACTGCTGCGCGGCAGCCGAC GCCAGGTTGGAGCAGGGGGGCTGCACCAACGACCGCGGAGAGCTGGAGCATCCTGGCATCACCGCGCAAC CTGTCTATGTCCCCTTCCTGATCGTCGGCTCCATCTTCATCGCCTTCATCATTTTGGGCTCTTTAGTGGC TATCTATTGTTGCACCTGTTTGAGACCCAAGGAGCCCTCTCAGCAGCCCATCCGCTTCTCGCTCCGAAGC TATCAGACAGAGACCTTGCCCATGATCCTCACCTCCACCAGCCTCAGAGCAGCTTCCAGGCAGTCCAGCA CCGCTACCAGCTCCAGCTCCACGGGAGGATCTGTGCGGAGGTTCTCCTTTGCCAGGGCTGAGCCAAGCTG CCTGGTGCCCTCATCACCCCCACCTTACACCACTGGTCACACCATACACCTAACCCAACCGTCCGGTTTC CTGGTATCACCCCAGTATTTTGCATACCCTCTCCAGCAGGAGCCCCCATTACCTGGGAAGAGCTGTCCAG ATTTCAGTTCCAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216748 protein sequence
Red=Cloning site Green=Tags(s) MGALLAFCLLVGLLRWGPAGAQQPGEYCHGWVDAQGNYHEGFQCPEDFDTQDATICCGSCALRYCCAAAD ARLEQGGCTNDRGELEHPGITAQPVYVPFLIVGSIFIAFIILGSLVAIYCCTCLRPKEPSQQPIRFSLRS YQTETLPMILTSTSLRAASRQSSTATSSSSTGGSVRRFSFARAEPSCLVPSSPPPYTTGHTIHLTQPSGF LVSPQYFAYPLQQEPPLPGKSCPDFSSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001033415 |
ORF Size | 717 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001033415.3, NP_001028587.1 |
RefSeq Size | 3754 bp |
RefSeq ORF | 717 bp |
Locus ID | 330096 |
UniProt ID | Q3UPR0 |
MW | 25.6 kDa |
Gene Summary | Plays an essential role in the maturation of presomitic mesoderm cells by individual attenuation of both FGF and WNT signaling.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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The Shisa3 knockout mouse exhibits normal bone phenotype
,Murakami, K;Zhifeng, H;Suzuki, T;Kobayashi, Y;Nakamura, Y;,
J. Bone Miner. Metab.
,PubMed ID 31222549
[SHISA3]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC214574 | Shisa3 (untagged) - Mouse shisa homolog 3 (Xenopus laevis) (Shisa3), (10ug) |
CNY 3,990.00 |
|
MG216748 | Shisa3 (tGFP-tagged) - Mouse shisa homolog 3 (Xenopus laevis) (Shisa3), (10ug) |
CNY 2,850.00 |
|
MR216748L3 | Lenti ORF clone of Shisa3 (Myc-DDK-tagged) - Mouse shisa homolog 3 (Xenopus laevis) (Shisa3) |
CNY 4,750.00 |
|
MR216748L4 | Lenti ORF clone of Shisa3 (mGFP-tagged) - Mouse shisa homolog 3 (Xenopus laevis) (Shisa3) |
CNY 4,750.00 |