Rnls (NM_001146342) Mouse Tagged ORF Clone

CAT#: MR216631

  • TrueORF®

Rnls (Myc-DDK-tagged) - Mouse renalase, FAD-dependent amine oxidase (Rnls), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_001146342" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,040.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rnls Antibody - middle region
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 6530404N21Rik; AI452315; AW060440; C10orf59
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR216631 representing NM_001146342
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATTACTGCCAGCAGTCCTCATAATCCCCGATGCACAGCTGACTTGGGAGCTCAGTACATCACCTGCT
CTCCTCATTATGTCAAAGAGCACCAAAATTTTTATGAGGAACTGTTAGCTCATGGAATTTTGAAGCCTCT
GACATCCCCCATTGAAGGAATGAAAGGGAAGGAAGGAGATTGCAACTTTGTGGCACCTCAAGGATTTTCT
TCAGTTATCAAGTACTACTTGAAAAAGTCAGGTGCAGAAGTCTCCCTCAAGCACTGTGTGACTCAGATCC
ACCTGAAAGATAACAAGTGGGAAGTCTCCACAGACACTGGCTCTGCTGAGCAGTTTGACCTTGTCATCCT
CACCATGCCAGCTCCTCAGATTCTGGAACTTCAAGGTGACATTGTGAACTTAATTAGTGAACGCCAGAGG
GAGCAACTGAAATCTGTGAGCTACTCCTCTCGCTATGCTCTGGGCCTCTTTTATGAAGTAGGCATGAAGA
TTGGTGTCCCTTGGTCCTGCCGCTACCTCAGCAGTCACCCCTGCATATGCTTCATCTCCATTGATAATAA
GAAGCGCAATATAGAGTCATCAGAATGTGGTCCATCCGTGGTGATCCAAACCACTGTCCCATTTGGAGTT
CAACACTTGGAGGCCAGTGAGGCGGATGTGCAGAAGTTAATGATCCAGCAATTGGAAACCATTCTGCCGG
GTTTGCCTCAGCCAGTTGCTACCATATGCCATAAATGGACATATTCACAGGTTACAAGCTCAGTTTCCGA
CAGACCTGGTCAGATGACTCTTCATCTCAAGCCTTTCCTGGTGTGCGGAGGGGATGGATTTACTCACTCC
AACTTCAATGGCTGCATCTCCTCTGCCCTGAGTGTCATGAAAGTTTTAAAGCGTTATATT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR216631 representing NM_001146342
Red=Cloning site Green=Tags(s)

MITASSPHNPRCTADLGAQYITCSPHYVKEHQNFYEELLAHGILKPLTSPIEGMKGKEGDCNFVAPQGFS
SVIKYYLKKSGAEVSLKHCVTQIHLKDNKWEVSTDTGSAEQFDLVILTMPAPQILELQGDIVNLISERQR
EQLKSVSYSSRYALGLFYEVGMKIGVPWSCRYLSSHPCICFISIDNKKRNIESSECGPSVVIQTTVPFGV
QHLEASEADVQKLMIQQLETILPGLPQPVATICHKWTYSQVTSSVSDRPGQMTLHLKPFLVCGGDGFTHS
NFNGCISSALSVMKVLKRYI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001146342
ORF Size 900 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq Size 1383 bp
RefSeq ORF 549 bp
Locus ID 67795
UniProt ID A7RDN6
MW 20.7 kDa
Gene Summary Catalyzes the oxidation of the less abundant 1,2-dihydro-beta-NAD(P) and 1,6-dihydro-beta-NAD(P) to form beta-NAD(P)(+). The enzyme hormone is secreted by the kidney, and circulates in blood and modulates cardiac function and systemic blood pressure. Lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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