Scn4a (NM_133199) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	mH2; Nav1.4; SkM1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR216626 representing NM_133199 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCTCATCTCTGCCCACCCTGGTCCCCCCTGGTCCCCACTGCCTGCGCCCCTTCACCCCAGAGT CCCTGGCAGCCATAGAACAGCGGGCAATGGAGGAGGAAGCCCGGCTGCAGCGGAACAAGCAAATGGAGAT TGAAGAGCCTGAGCGGAAGCCACGCAGTGACCTGGAAGCTGGCAAGAACCTCCCACTCATCTATGGGGAC CCCCCACCTGAAGTCATTGGCGTCCCCCTGGAGGACCTGGACCCTTACTACAGTGACAAGAAGACCTTCA TTGTGCTCAACAAAGGAAAGGCCATCTTCCGATTCTCTGCCACCCCTGCCCTCTACATGCTGAGCCCCTT CAGCATCGTCCGGCGGGTGGCTATCAAGGTGCTCATCCACGCGCTGTTCAGCATGTTCATCATGATCACC ATCTTGACCAACTGCGTGTTCATGACCATGAGCAACCCGCCTTCTTGGTCCAAGGACGTGGAGTACACCT TCACAGGGATCTACACCTTTGAGTCCCTCATTAAGATGCTGGCCCGAGGCTTTTGCATTGATGACTTCAC ATTCCTCCGAGACCCCTGGAACTGGCTGGACTTCAGTGTCATCACAATGGCGTACGTGACAGAGTTTGTG GACTTGGGCAACATCTCAGCCCTGAGGACCTTCCGTGTGCTGCGGGCCCTGAAAACCATCACGGTCATCC CAGGGCTGAAGACGATCGTGGGAGCCCTGATCCAGTCTGTGAAAAAGCTCTCGGATGTGATGATCCTCAC TGTCTTCTGCCTGAGCGTCTTTGCCCTGGTGGGGCTGCAGCTTTTCATGGGAAACCTGCGTCAGAAGTGC GTGCGTTGGCCCCCGCCCATGAATGACACCAACACCACGTGGTATGGTAATGATACGTGGTACGGCAATG ACACCTGGTACGGCAATGACACCTGGTATGGCAATGACACTTGGAACAGCCAGGAGAGCTGGGTCAGCAA CTCTACCTTTGACTGGGAGGCCTACATCAATGACGAAGGGAACTTCTATTTCTTGGAGGGCTCCAATGAT GCCCTGCTCTGTGGGAATAGCAGTGATGCCGGGCACTGCCCTGAGGGCTACGAATGCATGAAGGCTGGGA GGAACCCCAACTATGGCTACACCAGCTACGACACCTTCAGCTGGGCCTTCCTGGCTCTCTTCCGTCTCAT GACGCAGGACTACTGGGAGAACCTTTTCCAGCTGACCCTTCGAGCCGCTGGCAAGACCTACATGATCTTC TTTGTGGTCATCATCTTCCTGGGTTCCTTCTACCTCATCAATCTGATCCTGGCGGTGGTGGCCATGGCAT ATGCTGAGCAGAATGAGGCTACCCTGGCCGAAGACCAGGAGAAAGAGGAGGAGTTCCAACAGATGCTTGA AAAATTCAAAAAACATCAGGAGGAACTGGAAAAGGCGAAGGCTGCCCAGGCTCTGGAAGGTGGAGAGGAG GCAGATGGGGACCCAACCCACAGCAAAGACTGCAATGGTAGCCTGGATACATCCGGGGAGAAGGGGCCCC CAAGGCCAAGCTGCAGCGCAGAGAGTGCCATCTCAGATGCTATGGAGGAGCTGGAAGAGGCCCATCAGAA GTGCCCCCCATGGTGGTACAAGTGTGCACACAAAGTGCTCATCTGGAACTGCTGTGCCCCGTGGGTGAAG TTCAAGCATATTATCCTCCTGATTGTCATGGACCCCTTTGTGGACCTGGGCATCACCATCTGCATCGTGC TCAACACCCTCTTCATGGCCATGGAGCACTACCCCATGACTGAGCACTTTGACAACGTGCTCTCTGTGGG CAACTTGGTCTTCACGGGCATCTTCACTGCAGAGATGGTGCTGAAGTTGATTGCTATGGACCCCTACGAA TATTTCCAACAGGGCTGGAACATCTTCGACAGCTTCATCGTCACCCTCAGCCTGGTGGAGCTGGGCCTGG CCAACGTGCAGGGCCTATCAGTGCTCCGTTCTTTCCGTCTGCTGCGTGTCTTCAAGCTGGCCAAGTCATG GCCAACACTCAACATGCTCATCAAAATCATTGGCAACTCAGTGGGTGCGCTGGGCAACCTGACCCTGGTG TTGGCCATCATCGTCTTCATCTTCGCCGTGGTGGGCATGCAGCTGTTTGGCAAGAGCTATAAGGAATGCG TGTGCAAGATTGCCTCAGACTGCAGCCTGCCCCGCTGGCACATGCACGATTTCTTCCACTCCTTCCTCAT CGTCTTCCGCATCCTCTGTGGGGAGTGGATCGAGACCATGTGGGACTGCATGGAAGTGGCCGGCCAGGCC ATGTGCCTCACCGTCTTCCTCATGGTCATGGTCATTGGCAACCTGGTGGTCCTGAATCTGTTCCTGGCTC TCCTGTTGAGTTCCTTCAGCGCTGACAGTCTGGCAGCGTCAGATGAAGATGGTGAGATGAACAACCTACA GATCGCCATCGGGCGTATCAAGTGGGGCATCGCCTTTGCCAAAACCTTCCTCTTGGGGCTGTTGCATGGC AAGATCCTGAGCCTCAAAGACATAATGCTCAGTCTCGGGGAGCCTGGGGGTGCTGGGGAAAACGGGGAGA GCCCTCCCGAGGATGAGAAGAAGGAACCACCTCCGGAAGATGGGAACAAGGAGCTGAAAGACAATCATAT CCTGAACCACGTGGGCCTGACTGATGGACCCCGCTCCAGCATCGAGATGGACCACCTTAACTTCATCAAC AACCCCTACCTCACTATCCATGTGCCCATTGCCTCCGAGGAGTCCGACTTGGAAATGCCCACAGAGGAGG AGACTGACACCTTCTCGGAGCCTGAGGACATCAAGAAGCCACTACAGCCTCTGTACGACGGGAACTCCTC CGTCTGCAGCACAGCTGACTACAAGCCCCCCGAAGAGGACCCTGAGGAGCAGGCTGAGGAGAACCCTGAG GGGGAGCTACCTGAGGAGTGTTTCACAGAGGCCTGTGTGAAGCGCTGCCCCTGCCTCTATGTGGACATCT CCCAGGGCCGCGGGAAAATGTGGTGGACACTCCGCAGGGCCTGCTTCAAGATTGTTGAGCACAACTGGTT TGAGACCTTCATCGTCTTCATGATCTTGCTCAGCAGCGGGGCCCTGGCCTTCGAGGACATCTACATTGAA CAGCGGCGAGTCATCCAAACCATCCTGGAATATGCTGACAAGGTCTTCACCTACATCTTCATCCTGGAGA TGCTGCTAAAGTGGGTGGCCTACGGCTTCAAGGTGTACTTCACCAACGCCTGGTGCTGGCTCGACTTCCT CATTGTGGATGTCTCCATCATCAGTCTGGTGGCTAACTGGCTGGGCTACTCTGAGCTGGGGCCCATCAAA TCCCTGCGCACGCTCCGGGCCCTGCGACCTCTGAGGGCACTGTCCCGATTTGAAGGCATGAGGGTGGTGG TGAACGCCCTTCTGGGAGCCATCCCTTCCATCATGAACGTGCTCCTTGTCTGCCTCATCTTCTGGCTCAT CTTCAGCATCATGGGGGTCAACTTGTTTGCTGGAAAGTTCTACTACTGCATCAACACAACCACCTCCGAG AGATTCGACATCTCTGTGGTCAACAACAAGTCCGAGTGTGAGAGCCTCATGTACACCGGCCAGGTCCGCT GGATGAACGTCAAGGTCAACTATGACAACGTGGGTCTCGGCTACCTCTCTCTTCTTCAGGTGGCCACTTT CAAGGGTTGGATGGACATCATGTATGCAGCTGTGGACTCCCGGGAGAAAGAGGAGCAGCCAGACTACGAG GTGAACCTCTACATGTACCTTTACTTCGTCATCTTTATCATCTTCGGCTCCTTCTTCACCCTCAATCTCT TCATTGGCGTCATCATTGACAACTTCAACCAACAGAAGAAGAAGTTTGGAGGGAAAGACATCTTCATGAC AGAGGAACAGAAGAAATACTACAATGCGATGAAGAAGCTTGGCTCCAAGAAGCCCCAGAAGCCAATCCCC CGGCCTCAGAACAAGATCCAAGGCATGGTGTACGACTTCGTGACAAAGCAGGTGTTTGACATCTCTATCA TGATCCTCATCTGCCTCAACATGGTGACGATGATGGTGGAGACGGATGACCAGAGCCAGCTCAAGGTGGA CATCCTGTATAACATCAACATGGTCTTCATTATCGTCTTCACAGGGGAGTGTGTGCTCAAGATGTTTGCC CTGCGCCATTACTACTTCACCATTGGCTGGAATATCTTCGACTTCGTGGTTGTCATCTTGTCCATTGTGG GTCTCGCACTCTCTGACTTGATACAGAAATACTTTGTGTCACCCACACTGTTCCGTGTGATCCGATTGGC TCGGATCGGGCGTGTCTTGCGTCTGATCCGTGGGGCCAAGGGCATCCGGACGCTGCTGTTCGCCCTGATG ATGTCCCTGCCCGCCCTCTTCAACATCGGCCTCCTTCTCTTTCTGGTCATGTTCATTTACTCCATCTTCG GCATGTCTAACTTCGCCTACGTCAAGAAAGAGTCGGGCATCGACGACATGTTCAACTTTGAGACCTTTGG CAACAGTATCATCTGCCTCTTCGAGATCACCACGTCAGCTGGCTGGGACGGGCTTCTGAACCCCATCCTC AACAGTGGGCCCCCAGACTGTGACCCGACATTGGAGAACCCAGGCACCAATATCAAGGGTGACTGCGGCA ACCCATCCATCGGCATCTGTTTCTTCTGCAGCTACATCATCATCTCCTTCCTCATCGTGGTCAACATGTA CATTGCCATCATCCTAGAGAATTTCAATGTGGCCACTGAAGAGAGCAGTGAGCCCCTCTGTGAGGATGAC TTCGAGATGTTCTATGAGACCTGGGAGAAGTTTGACCCCGATGCAACTCAGTTCATCGACTACAGCCGCC TTTCTGACTTTGTGGACACCCTGCAGGAGCCCTTGAAAATTGCCAAGCCCAATAAGATCAAGCTTATCAC ATTAGACCTGCCCATGGTACCCGGGGACAAGATCCACTGCCTGGACATCCTCTTTGCCCTGACCAAAGAG GTACTGGGTGACTCTGGGGAAATGGATGCTCTCAAACAGACCATGGAGGAGAAGTTCATGGCAGCTAACC CCTCCAAGGTCTCCTATGAGCCCATCACCACCACCCTCAAGAGGAAGCAGGAGGAGGTGTGTGCTATCAA AATCCAGAGGGCTTACCGCCGCCATCTGCTGCAGCGCTCTGTGAAGCAGGCCTCTTACATGTACCGCCAC AGCCAGGAAGGCAATGGCGATGGGGCCCCTGAGAAGGAGGGATTGCTTGCCAACACCATGAACAAGATGT ATGGCTCTGAGAAAGAGGACAACGGTGTGCAGAGCCAGGGGGAGAAAGAGAAAGACTCAACAGAGGATGC TGGACCCACCACGGAGGTCACAGCCCCCAGCAGCTCGGACACTGCCCTGACTCCTCCTCCTCCTTCTCCT CCTCCACCATCATCACCACCACAAGGGCAGACAGTGCGCCCAGGGGTCAAAGAGTCTCTTGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR216626 representing NM_133199 Red=Cloning site Green=Tags(s) MASSSLPTLVPPGPHCLRPFTPESLAAIEQRAMEEEARLQRNKQMEIEEPERKPRSDLEAGKNLPLIYGD PPPEVIGVPLEDLDPYYSDKKTFIVLNKGKAIFRFSATPALYMLSPFSIVRRVAIKVLIHALFSMFIMIT ILTNCVFMTMSNPPSWSKDVEYTFTGIYTFESLIKMLARGFCIDDFTFLRDPWNWLDFSVITMAYVTEFV DLGNISALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALVGLQLFMGNLRQKC VRWPPPMNDTNTTWYGNDTWYGNDTWYGNDTWYGNDTWNSQESWVSNSTFDWEAYINDEGNFYFLEGSND ALLCGNSSDAGHCPEGYECMKAGRNPNYGYTSYDTFSWAFLALFRLMTQDYWENLFQLTLRAAGKTYMIF FVVIIFLGSFYLINLILAVVAMAYAEQNEATLAEDQEKEEEFQQMLEKFKKHQEELEKAKAAQALEGGEE ADGDPTHSKDCNGSLDTSGEKGPPRPSCSAESAISDAMEELEEAHQKCPPWWYKCAHKVLIWNCCAPWVK FKHIILLIVMDPFVDLGITICIVLNTLFMAMEHYPMTEHFDNVLSVGNLVFTGIFTAEMVLKLIAMDPYE YFQQGWNIFDSFIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLV LAIIVFIFAVVGMQLFGKSYKECVCKIASDCSLPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQA MCLTVFLMVMVIGNLVVLNLFLALLLSSFSADSLAASDEDGEMNNLQIAIGRIKWGIAFAKTFLLGLLHG KILSLKDIMLSLGEPGGAGENGESPPEDEKKEPPPEDGNKELKDNHILNHVGLTDGPRSSIEMDHLNFIN NPYLTIHVPIASEESDLEMPTEEETDTFSEPEDIKKPLQPLYDGNSSVCSTADYKPPEEDPEEQAEENPE GELPEECFTEACVKRCPCLYVDISQGRGKMWWTLRRACFKIVEHNWFETFIVFMILLSSGALAFEDIYIE QRRVIQTILEYADKVFTYIFILEMLLKWVAYGFKVYFTNAWCWLDFLIVDVSIISLVANWLGYSELGPIK SLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCINTTTSE RFDISVVNNKSECESLMYTGQVRWMNVKVNYDNVGLGYLSLLQVATFKGWMDIMYAAVDSREKEEQPDYE VNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGKDIFMTEEQKKYYNAMKKLGSKKPQKPIP RPQNKIQGMVYDFVTKQVFDISIMILICLNMVTMMVETDDQSQLKVDILYNINMVFIIVFTGECVLKMFA LRHYYFTIGWNIFDFVVVILSIVGLALSDLIQKYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALM MSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDMFNFETFGNSIICLFEITTSAGWDGLLNPIL NSGPPDCDPTLENPGTNIKGDCGNPSIGICFFCSYIIISFLIVVNMYIAIILENFNVATEESSEPLCEDD FEMFYETWEKFDPDATQFIDYSRLSDFVDTLQEPLKIAKPNKIKLITLDLPMVPGDKIHCLDILFALTKE VLGDSGEMDALKQTMEEKFMAANPSKVSYEPITTTLKRKQEEVCAIKIQRAYRRHLLQRSVKQASYMYRH SQEGNGDGAPEKEGLLANTMNKMYGSEKEDNGVQSQGEKEKDSTEDAGPTTEVTAPSSSDTALTPPPPSP PPPSSPPQGQTVRPGVKESLV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_133199
ORF Size	5523 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_133199.2, NP_573462.2
RefSeq Size	6598 bp
RefSeq ORF	5526 bp
Locus ID	110880
UniProt ID	Q9ER60
MW	209.2 kDa
Gene Summary	Pore-forming subunit of a voltage-gated sodium channel complex through which Na(+) ions pass in accordance with their electrochemical gradient. Alternates between resting, activated and inactivated states (PubMed:11834499). Required for normal muscle fiber excitability, normal muscle contraction and relaxation cycles, and constant muscle strength in the presence of fluctuating K(+) levels (PubMed:18317596, PubMed:21881211).[UniProtKB/Swiss-Prot Function]

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