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Vps51 (NM_001081041) Mouse Tagged ORF Clone
CAT＃: MR216602
- TrueORF®
Vps51 (Myc-DDK-tagged) - Mouse RIKEN cDNA 1110014N23 gene (1110014N23Rik)
ORF Plasmid: tGFP
"NM_001081041" in other vectors (4)
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CNY 5,728.00
CNY 6,840.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110014N23Rik; 3110057M17Rik; AI837850; Ffr |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR216602 representing NM_001081041
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCTGCGGCAGCTGTGGGGCCTGGCCTAGGGTCCGGGCCTGGAGACTCCCCAGAAGGTCCCGAGG CGGACGCTCCGGAGCGTCGGCGGAAGGCTCACGGGATGCTGAAGCTTTACTACGGCCTCTCGGAGGGGGA GGTGGCGGGGCATCCCGCAGGGCCTGACCCTCTGGACCCAACCGATCTCAATGGGGCGCACTTCGACCCG GAAGTTTATCTTGACAAGCTGCGTAGAGAATGTCCTCTGGCCCAGCTGATGGACAGTGAGACGGACATGG TACGGCAGATCCGGGCTCTAGACAGCGACATGCAGACCCTGGTCTATGAGAACTACAACAAGTTCATCTC AGCTACAGACACCATTCGAAAGATGAAGAATGACTTCCGGAAGATGGAAGATGAGATGGACCGGCTGGCC ACCAACATGGCAGTGATCACCAACTTCAGCGCACGGATCAGCGCCACGCTACAGGACCGACACGAGCGCA TCACCAAGCTAGCAGGGGTACATGCACTACTGCGGAAGCTGCAGTTCCTTTTTGAGCTGCCTTCGCGCCT CACCAAGTGTGTGGAGCTGGGTGCCTATGGGCAGGCAGTGCGCTATCAGGGTCGTGCCAGGGCCGTGCTG CAGCAGTACCAGCACCTGCCCTCCTTCCGTGCCATACAGGATGACTGCCAGGTCATCACAGCTCGCTTGG CCCAGCAGCTGCGGCAGCGCTTCAGGGAAGGCTGCTCAGGTGCCCCGGAGCAGGCCGAGTGTGTGGAGCT GCTGCTGGCCCTGGGCGAGCCAGCTGAGGAGCTGTGCGAGGAGTTCCTGGCGCATGCGCGGGGACGGCTG GAGGAAGAACTGAGCAGCCTGGAGGCCGAACTGGGGCCCTCCCCTCCAGCACCTGATGTGTTAGAGTTCA CCGACCGTGGTGGCAATGGTTTTGTAGGTGGCCTCTGCCAGGTGGCAGCAGCCTACCAAGAGCTGTTTGC TGCTCAGGGCCCTGCTGGTGCGGAGAAGCTGGCAGCCTTTGCGCAGGAGCTGGGCGGCCGCTACTTTGCA CTGGTTGAGCGGCGGCTGGCACAGGAGCAGGGTGGTAGTGACAACTCACTGCTGGTGCGGGCACTAGATC GCTTCCACCGGCGCCTGCGAGCACCTGGGGCCCTGCTGGCCGCTGCTGGGCTTTCAGAGTCAGCCACAGA GATTGTGGAGCGAGTGGCCCGGGAGCGCCTGAGCCACCATCTGCAGGGTCTGAAGGCTGCCTTCCTGAGC TCCCTGACGGATGTTCGCCAGGCCCTGGCAGCACCTCGCCTAGCGGGGAAGGAGGGCCCCAGCCTGGCTG AGTTGCTGGCCAATGTGGCCAGCTCCATCCTAAGCCACATCAAGACGTCACTGGCTTCTGTGCATCTCTT TACCGCCAAGGAGGTGTCCTTCTCCAATAAGCCCTACTTCAGGGGCGAGTTCTGCAGCCAGGGAGTTCGT GAGGGCCTCATCGTGGGTTTCATCCGCTCCATGTGCCAGACAGCCCAGAGCTTCTGTGACAGCCCTGGAG AAAAGGGAGGTGCCACGCCGCCTGCCCTGCTCCTGCTGCTCTCTCGCCTCTGCCTGGATTATGAGACAGC CACCATCTCCTACATCCTCACACTCACTGATGAACAGTTTCTGGTGCAGGACCAGTCTCCCGTTACACCC GTGAGCACACTGTGTGCAGAGGCCAGAGAGACAGCACGGCGGCTGCTGACTCACTATGTGAAGGTGCAAG GCCTGGTCATATCCCAGATGCTACGTAAGAGTGTGGAGACCCGGGACTGGCTCAGTACTCTGGAGCCTAG GAACGTGCGTGCTGTCATGAAGCGTGTGGTGGAGGACACTACAGCTATTGATGTCCAGGTGGGGCTCCTG TATGAAGAGGGTGTCCGGAAGGCCCAGAGCAGTGACTCAAGCAAAAGGACTTTCTCTGTGTACAGCAGCT CCCGGCAGCAGGGCCGCTATGCACCCAGCTACACACCCAGTGCTCCCATGGACACAAACCTCCTGAGCAA TATTCAGAAGCTGTTCTCTGAACGGATCGATGTGTTCAGCCCCGTGGAATTCAACAAGGTGTCGGTGCTG ACTGGCATCATTAAGATCAGCTTGAAGACACTACTGGAGTGTGTGCGCCTACGCACCTTTGGGCGCTTTG GGCTGCAGCAGGTGCAGGTGGACTGCCACTTCCTGCAGCTTTACTTGTGGCGCTTTGTGGCTGATGAGGA GCTAGTGCACCTGCTGCTGGACGAAGTAGTAGCCTCAGCTGCTCTGCGCTGTCCCGACCCAGTGCCCATG GAACCTAGTGTTGTCGAGGTCATCTGCGAGCGCGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216602 representing NM_001081041
Red=Cloning site Green=Tags(s) MAAAAAVGPGLGSGPGDSPEGPEADAPERRRKAHGMLKLYYGLSEGEVAGHPAGPDPLDPTDLNGAHFDP EVYLDKLRRECPLAQLMDSETDMVRQIRALDSDMQTLVYENYNKFISATDTIRKMKNDFRKMEDEMDRLA TNMAVITNFSARISATLQDRHERITKLAGVHALLRKLQFLFELPSRLTKCVELGAYGQAVRYQGRARAVL QQYQHLPSFRAIQDDCQVITARLAQQLRQRFREGCSGAPEQAECVELLLALGEPAEELCEEFLAHARGRL EEELSSLEAELGPSPPAPDVLEFTDRGGNGFVGGLCQVAAAYQELFAAQGPAGAEKLAAFAQELGGRYFA LVERRLAQEQGGSDNSLLVRALDRFHRRLRAPGALLAAAGLSESATEIVERVARERLSHHLQGLKAAFLS SLTDVRQALAAPRLAGKEGPSLAELLANVASSILSHIKTSLASVHLFTAKEVSFSNKPYFRGEFCSQGVR EGLIVGFIRSMCQTAQSFCDSPGEKGGATPPALLLLLSRLCLDYETATISYILTLTDEQFLVQDQSPVTP VSTLCAEARETARRLLTHYVKVQGLVISQMLRKSVETRDWLSTLEPRNVRAVMKRVVEDTTAIDVQVGLL YEEGVRKAQSSDSSKRTFSVYSSSRQQGRYAPSYTPSAPMDTNLLSNIQKLFSERIDVFSPVEFNKVSVL TGIIKISLKTLLECVRLRTFGRFGLQQVQVDCHFLQLYLWRFVADEELVHLLLDEVVASAALRCPDPVPM EPSVVEVICERG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001081041 |
| ORF Size | 2346 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001081041.1, NP_001074510.1 |
| RefSeq Size | 2631 bp |
| RefSeq ORF | 2349 bp |
| Locus ID | 68505 |
| UniProt ID | Q3UVL4 |
| MW | 86.2 kDa |
| Gene Summary | Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of protein retrieval from endosomes to the TGN, acid hydrolase sorting, lysosome function, endosomal cholesterol traffic and autophagy. VPS51 participates in retrograde transport of acid hydrolase receptors, likely by promoting tethering and SNARE-dependent fusion of endosome-derived carriers to the TGN. Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221574 | Vps51 (untagged) - Mouse RIKEN cDNA 1110014N23 gene (1110014N23Rik), (10ug) |
CNY 10,070.00 |
|
| MG216602 | Vps51 (tGFP-tagged) - Mouse RIKEN cDNA 1110014N23 gene (1110014N23Rik), (10ug) |
CNY 7,510.00 |
|
| MR216602L3 | Lenti ORF clone of Vps51 (Myc-DDK-tagged) - Mouse RIKEN cDNA 1110014N23 gene (1110014N23Rik) |
CNY 8,740.00 |
|
| MR216602L4 | Lenti ORF clone of Vps51 (mGFP-tagged) - Mouse RIKEN cDNA 1110014N23 gene (1110014N23Rik) |
CNY 8,740.00 |
